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MYOGRAPH SYSTEM - 114PB
USER MANUAL VOL. 1.7
Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk

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Summary of Contents for DMT MYOGRAPH SYSTEM 114PB

  • Page 1 MYOGRAPH SYSTEM - 114PB USER MANUAL VOL. 1.7 Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 2: Trademarks

    Every attempt ensures accurate information, misprints, and construction- and specification changes can occur. Danish Myo Technology A/S reserves the right to alter/change content as required and without any notice. Copyright © Danish Myo Technology A/S Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 3: Table Of Contents

    3.3 MAIN MENU 3.4 PRESSURE MENU 3.5 HEAT MENU 3.6 TIMER AND BUZZER MENU 3.7 SETTING MENU APPENDIX 1 - SYSTEM SPECIFICATIONS PRESSURE MYOGRAPH SYSTEM - 114PB Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 4: Safety

    Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 5: Ec Declaration Of Conformity

    Do not use this device in close proximity to sources of strong electromagnetic radiation (e.g., unshielded intentional RF sources), as these may interfere with the proper operation. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 6: Unpacking The Pressure Myograph System

    We recommend that you store the packing material for any possible future transport of the Pressure Myograph System. In case of shipping and the original packing material is unavailable, please contact the DMT Sales Department for advice and packing instructions. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 7: Chapter 1 - System Overview

    CHAPTER 1 - SYSTEM OVERVIEW 1.1 INTERFACE Touch display Power indicator 1x500ml Schott Duran bottles Figure 1.1 Pressure Interface - front Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 8 Inlet for oxygen/garbogen Myograph chamber to pressure bottles (max 1bar) ON/OFF switch Analogue recorder output - connection to external data acquisition Figure 1.2 Pressure Interface - rear Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 9: Pressure Myograph Chamber

    The position of the mounting steel pin Figure 1.3 Pressure Myograph – 114PB chamber and a close-up detail of the chamber Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 10 A camera mount is needed for the DMT USB camera. Contact the DMT Sales Department for further product information and price on any optional equipment, including microscopes. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 11: Chapter 2 - System Setup

    BETWEEN 0.5BAR and 1.0 BAR. 3. Microscope/camera to computer connection: A. DMT Inverted Microscope: Inside the DMT Microscope, there is a USB camera wired to a USB cable and one USB cable to power the microscope light. Connect the two USB cables from the DMT Microscope to the computer USB ports.
  • Page 12: External Pressure Myograph Connections

    Gas supply to the chamber coverlid (Optional): Connect the Oxygen/Carbogen supply (Tank/Cylinder) to the DMT Gas Supply Manifold. One of the lines from the Manifold is connected to the 114PB Coverlid (figure 2.1 and figure 2.2). Regulate the gas supply to the chamber using the screw on the Gas Supply Manifold or a similar gas regulator.
  • Page 13 (a 2mm tube is not included with the Pressure Myograph System). The Vacuum Package (#101018/#101052) contains the electronic valve, vacuum waste bottle, vacuum pump, and tubing and fittings. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 14: The First Pressure Calibration

    DMT recommends that new pressure calibrations b e performed before the first use. The pressure calibration procedures are described in detail in chapter 3.7.1 Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 15: Chapter 3 - Pressure Interface Menus

    The selected line will turn black. ENTER Pressing the white X in the red box will exit the menu and take you automatically to the Main Menu. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 16: Power-Up Screen

    See the next page for a description of the Main Menu. Four sub-menus are accessible from the Main Menu screen: Pressure Menu, Heat Menu, Timer Buzzer Menu, and Setting Menu. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 17 This icon indicates the status of the chamber heat. If the icon is grey, the heat is OFF. If the icon is green, the heat is ON (active). Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 18: Pressure Menu

    To turn the pressure regulator on, press ON outside the line for Pressure. When activated, it turns green. In Main Menu, the pressure icon also turns green when pressure regulation is on. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 19: Heat Menu

    The system will heat to the desired temperature Temperature setpoint. 37.0 °C setpoint. Probe temperature. 36.6 °C In Main Menu the thermometer icon turns green when HEAT: the heat is on. DEFAULT ENTER Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 20: Timer And Buzzer Menu

    To activate the Buzzer, press ON, and it will turn green. In SET TIMER Main Menu, the bell icon also turns green. Set Timer: 00 : 00 : 00 SELECT Timer: Buzzer: CLR TIMER ENTER Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 21: Setting Menu

    114PB Pressure Myograph System. The calibration procedure is listed in 5 individual steps and needs to be performed one at a time to calibrate the system. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 22 5. Close the P1 3-way valve as shown in figure 3.2. The inlet to P1 now comes from the pressure manometer, and the Pressure Myograph system is now ready for a calibration procedure. Calibration tube Connect the Calibration tube here Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 23 Pressure Myograph using the Big Ben Pressure Manometer. When the blue line of relative values stabilizes, go to NEXT STEP. The relative values should be between 3300 to 3900 Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 24 The values must be 200.0 +/-1.0mmHg. Carefully remove the calibration shunt tube and reconnect the tubing to the glass cannulas. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 25 Pressing ENTER will save the values for future experiments. 3.7.2 PH CALIBRATION PROCEDURE (OPTIONAL) The pH Module in the 114PB is locked by a code. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 26 See the sub-menu pH Set- up (3.6.5.2 pH Set-up Menu). pH Setup menu The temperature is an important parameter in calibration formula obtained automatically if AUTO is selected in the Temperature compensation function. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 27 (here pH 7) and turn on the stirring of the high buffer solution. When the relative pH output in the blue line is stable, go to NEXT STEP. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 28 The analog output works in a range of -2,5V to +2,5V. Use SELECT to select the channel number to be Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 29 ENTER access to diagnostics panels that provide information during a malfunction or mechanisms to change other settings controlled by the onboard computer. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 30: Appendix 1 - System Specifications

    ±0.5mmHg Resolution 0.1mmHg RESERVOIR: Heated Capacity 250ml Pressure circuit Closed Air inlet 1bar (max) OUTPUT: USB 2.0 Data communication Analog output channels Analog output range ±2.5V Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
  • Page 31 Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...