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Summary of Contents for DMT 360CW
- Page 1 USER MANUAL, VOL.2.0 W I R E MYOG RA PH SYSTEM - 3 60 CW & 320A...
- Page 2 WIRE MYOGRAPH SYSTEMS 360CW AND 320A USER MANUAL User Manual Version 1.0...
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Page 3: Trademarks
New information may be supplied separately. This documentation is provided with the Wire Myograph system No part of this document may be reproduced by any means without the prior written permission of DMT A/S. Copyright © Danish Myo Technology A/S... -
Page 4: Table Of Contents
Chapter 1 - System overview ........................................6 1.1 Wire Interface - front and rear ......................................6 1.2 Wire Myographs ............................................7 1.2.1 Confocal Wire Myograph - 360CW .....................................7 1.2.2 Single Wire Myograph - 320A ......................................8 Chapter 2 - Setting up ..........................................9 2.1 Setting up the complete Wire Myograph System ...............................9 2.2 The first force calibration ........................................ -
Page 5: Safety
EN 61010-2-030:2010 General warnings regarding EMC: Do not use this device in close proximity to sources of strong electromagnetic radiation (e.g. unshielded intentional RF sources), as these may interfere with the proper operation. WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL... - Page 6 Please take a few minutes to carefully inspect your new Wire Myograph System for damage which may have occurred during handling and shipping. If you suspect any kind of damage, please contact DMT immediately and the matter will be pursued as quickly as possible.
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Page 7: Chapter 1 - System Overview
(optional) Data Acquisition ON/OFF switch 24V DC port for power USB connection port Connection port Connection port for for computer for pH meter Wire Myograph (optional) Figure 1.2 Wire Interface - rear WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL... -
Page 8: Wire Myographs
Wire jaw connected to Wire jaw connected to force transducer horizontal micrometer Force transducer pin Window in the base of the chamber for imaging Figure 1.3 Confocal Wire Myograph - 360CW with close-up of the chamber CHAPTER 1... -
Page 9: Single Wire Myograph - 320A
Wire jaw connected to Wire jaw connected to force transducer micrometer Window at the bottom of the chamber for imaging (under the wire jaws) Figure 1.1 Single Wire Myograph with close-up of chamber WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL... -
Page 10: Chapter 2 - Setting Up
CHAPTER 2 - SETTING UP 2.1 Setting up the complete Wire Myograph System Wire Interface rear panel pH Meter Power supply Wire Myograph Data Acquisition System via BNC cables or USB Oxygen/ Electronic vacuum valve Carbogen/ Compressed air Gas supply manifold Vacuum Pump Suction Bottle Figure 2.1 Example of a complete Wire Myograph System - 320A... -
Page 11: The First Force Calibration
5. Suction connection: Connect the suction pipe on the Confocal Wire Myograph 360CW chamber cover (see figure 2.3) to a vacuum pump via a suction bottle and the electronic vacuum valve (optional). For the Wire Myograph 320A connect the suction pipe on the Wire Myograph side, see figure 2.4. -
Page 12: Chapter 3 - The Wire Interface Menus
The login screen appears after a few seconds if it is a new unregistered Wire Interface. If already registered the Main Menu display will appear. Before the Wire Interface can be used you need a login code. Go to the DMT website www.dmt.dk... -
Page 13: Main Menu
Heat Menu Timer/Alarm Menu Setting Menu 3.4 Zero Menu Is used to zero the output from the force transducer. Pressing ZERO will reset the baseline of the chart traces without affecting the calibrations. WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL... -
Page 14: Heat Menu
3.5 Heat Menu The heating temperatures are controlled from this menu. To turn the heat on, or to change the temperature for the system, press HEAT in the Main Menu. The Heat Menu will be displayed allowing the user to change the system temperature, as well as turn the heat on or off. -
Page 15: Settings Menu
• Place the calibration bridge and T-balance on the Wire Myograph (see figure 3.1 for 360CW or 3.2 for 320A) allowing it to be warmed up together with the Wire Myograph. Turn on the heating in the Heat sub-menu to the appropriate temperature used during your experiments •... - Page 16 Figure 3.1 Force calibration set-up - showing placement of the calibration bridge and T-balance (with weight in place) for Wire Myograph - 360CW The 2 g weight is placed on the pan Figure 3.2 Force calibration set-up - showing placement of the calibration bridge and T-balance...
- Page 17 37°C with the chamber cover in place. Once the chamber are heated and have reached the target temperature, press NEXT STEP. Figure 3.3 Illustration of how to fit the T-balance pin between the wire and the gap in the jaw support WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL...
- Page 18 THAN 2000 OR GREATER THAN 4500 BUT STILL A FOUR DIGIT NUM- BER, THE FORCE TRANSDUCER IS BROKEN BUT SHOULD BE ABLE TO BE REPAIRED BY A TEMPERATURE COMPENSATION DONE BY DMT. IF THE MESSAGE OFF IS DISPLAYED ON THE MAIN PAGE OF THE INTER- FACE,...
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Page 19: Ph Calibration Procedure
Step 3 - Place the pH electrode and temperature probe in the low buffer solution (here pH 4) and turn on stirring. When the relative pH output in the blue line is stable, press NEXT STEP. WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL... -
Page 20: Select Analog Output (Optional)
Step 4 - The calibration is now finished. The readings in the two bottom lines are the actual pH and temperature readings. 3.7.3 Select Analog Output (optional) The Analog Output Menu determines what will be sent to the BNC analogue output (REC 1, REC 2 REC 3, REC 4 at the back of the Wire Interface). -
Page 21: Interface Settings
Entering the proper five digit login code however will allow the trained technician access to diagnostics panels that will provide information during a malfunction, or mechanisms to change other settings controlled by the onboard computer. WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL... -
Page 22: Appendix 1 - System Specifications
Environmental humidity: 20% to 80% RH, none condensing. Environmental operating temperature: 15 to 40°C. Environmental storage temperature: 4 to 70°C. Technical specifications - Confocal Wire Myograph - 360CW Vessel size: >60 μm Vessel alignment: Manually X, Y & Z settings Chamber:... - Page 23 Optional accessories Enable pH-meter on the Wire Interface - range: pH 0 - 14 - temp. correction: 0 - 50°C Electronic valve: 100 to 240 VAC (auto) 50/60 Hz via external power supply WIRE MYOGRAPH SYSTEM - 360CW/320A - USER MANUAL...
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Page 24: Notes
NOTES NOTES...
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