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Summary of Contents for DMT 620M
- Page 1 MYOGRAPH SYSTEM - 620M USER MANUAL VOL. 3.1 Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
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Page 2: Trademarks
Changes may have been made to the software and hardware it describes since then. New information may be supplied separately. This documentation is provided with the DMT Multi Wire Myograph System – Model 620M All rights reserved. No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A/S. -
Page 3: Table Of Contents
4.1 Changing and adjusting the mounting supports 4.2 Calibration of the force transducer 4.3 Checking the force transducer 4.4 Force Transducer Replacement 4.5 Myograph Maintenance APPENDIX 1 - SYSTEM SPECIFICATIONS Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk... -
Page 4: Introduction
Confocal Myograph 120CW. In 2000, J. P. Trading changed its company structure and became known as DMT - Danish Myo Technology A/S. Today, DMT is one of the world’s leading designers and manufacturers of wire myographs, pressure myographs, culture myographs and organ/tissue baths. Driven by our global customer base, our singular goal is to develop and manufacture first-class research equipment within the fields of physiology and pharmacology. -
Page 5: Safety
SAFETY The 620M Multi Wire Myograph System has been designed for use only in teaching and research applications. It is not intended for clinical or critical life-care use and should never be used for these purposes, or for the prevention, diagnosis, curing, treatment, or alleviation of disease, injury, or handicap. -
Page 6: Emc/Emi
Connect the equipment into an outlet on a circuit different to that which the receiver is connected to. • Consult the dealer or an experienced radio/TV technician for help. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk... - Page 7 Do not use this device in close proximity to sources of strong electromagnetic radiation (e.g. unshielded intentional RF sources), as these may interfere with the proper operation. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
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Page 8: Unpacking The Myograph System
UNPACKING THE MYOGRAPH SYSTEM Take a few minutes to carefully inspect your new Multi Wire Myographs System - 620M for damage which may have occurred during handling and shipping. If you suspect any kind of damage, please contact DMT immediately and the matter will be pursued soon as possible. If the packing material appears damaged, please retain it until a possible claim has been settled. -
Page 9: Chapter 1 - System Overview
CHAPTER 1 - SYSTEM OVERVIEW INTERFACE FRONT PANEL Myo-Interface display Heat indicator Power indicator Valve buttons Figure 1.1 Interface front panel Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk... -
Page 10: Interface Rear Panel
USB output ON/OFF switch (digital signal) Grounding connected to 4 Recorder outputs analog Data Acquisition (analogue signal) Power connector Vacuum input Figure 1.2 Interface Rear Panel Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk... -
Page 11: Multi Wire Myograph Unit
Myograph jaw connected to force transducer Supports Figure 1.4 Close up of a myograph jaws ‘ Figure 1.6 Mounting pins for larger vessels Figure 1.5 Mounting jaws for small vessels Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk... -
Page 12: Chapter 2 - Setting Up
CHAPTER 2 - SETTING UP 2.1 THE COMPLETE MYOGRAPH 620M SYSTEM Digital data acquisition (DMT device enabler) Figure 2.1 The complete Multi Myograph System – Model 620M Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk... -
Page 13: Setting Up Step-By-Step
2.1. 1. Interface – PC Connection: Data acquisition is possible either by connecting the 620M Interface directly to a PC with Labchart Pro and the DMT Device enabler installed or through the BNC connectors and BNC cables from the 620M interface e.g. - Page 14 SLIDES – THE BROWN GREASE) AND TURNED AT REGULAR INTERVALS TO PREVENT THEM FROM STICKING OR PERMANENTLY FREEZING. Suction Temperature probe Funnel For drug application Figure 2.1 Suction connection Figure 2.3 Chamber cover Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 15 2.3 THE FIRST FORCE TRANSDUCER CALIBRATION Prior to the shipment of the Multi Wire Myograph 620M System, has gone through two days of continuous testing, including a final force transducer calibration. However, DMT recommends that a new force transducer calibration is performed before using the myograph system for the first time. The force transducer calibration procedure is described in detail in the FORCE CALIBRATION sub-menu under SETTINGS, as explained in Chapter 3.
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Page 16: Chapter 3 - The Interface Menus
CHAPTER 3 - THE INTERFACE MENUS Chapter 3 is a complete manual for the 620M Interface. The chapter contains a detailed description of how to navigate the touch-screen menus and how to use the special features of the 620M myograph. - Page 17 Force chamber 1: -2.3 mN Force chamber 2: -0.2 mN Force chamber 3: +1.0 mN Force chamber 4: -0.7 mN Probe temperature: 37.0 °C ZERO HEAT SETTINGS Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 18 FORCE REC. MEASUREMENT CALIBRATION CALIBRATION OUTPUT RANGE sub-menus include: INTERFACE SETTINGS 1. FORCE CALIBRATION 4. MEASUREMENT RANGE 2. pH CALIBRATION 5. INTERFACE SETTINGS 3. FORCE REC. OUTPUT Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 19 Press When ready go to next step. “NEXT STEP” when the calibration kit has been properly BACK NEXT STEP placed. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 20 4-digit number. Wait at least 30 to 40 seconds for the BACK NEXT STEP applied force to stabilize. When the 4-digit number has stabilized, then pressing “NEXT STEP”. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 21 BACK NEXT STEP 2. pH CALIBRATION MENU (OPTIONAL) SETTINGS The pH Module in the 620M is locked by a code. If the screen shown here below turn up a code is needed from DMT to FORCE FORCE REC. MEASUREMENT...
- Page 22 REC. OUT is 20 mN, meaning that if the force of the mounted Force4 Rec. Range. 1200 mN SELECT vessel exceeds 20 mN, the force recorded in the data ENTER acquisition software will Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 23 SELECT ENTER NOTE: FOR USERS USING THE ANALOG BNC OUTPUTS OF THE 620M INTERFACE FOR DATA ACQUISITION ON A POWERLAB OR SIMILAR SYSTEM: ANYTIME THE FORCE REC OUTPUT IS CHANGED, A NEW 2-POINT CALIBRATION MUST BE PERFORMED IN E.G. LABCHART TO ENTER THE NEW VOLTAGE VALUES INTO THE DATA ACQUISITION SYSTEM BEING USED.
- Page 24 NOTE: FOR DMT DEVICE ENABLER USERS LABCHART HAS TO BE RESTARTED AFTER CHANGING THE MEASUREMENT RANGE VALUES OTHERWISE THE OLD SETTING WILL PERSIST IN LABCHART AND THE FORCE READINGS WILL NOT BE CORRECT. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 25 1second, but 1second is usually not enough time to Chamber 3: 6 Sec. SELECT completely empty a chamber with even as small a volume of Chamber 4: 5 Sec. SELECT 5 ml. ENTER Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 26 Temperature comp.: MANUAL SELECT calibration buffers is measured with a thermometer and Manual temp. Value: 20 °C SELECT entered manually in the Manual Temp. value line. ENTER Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 27 SELECT when it is saved in the system. ENTER NOTE: FOR DMT DEVICE ENABLER USERS (NO POWERLAB) IT IS VERY IMPORTANT TO RESTART LABCHART PRO AFTER CHANGING THE FORCE UNITS OTHERWISE THE FORCE UNITS WILL NOT BE TRANSFERRED TO LABCHART.
- Page 28 It is important to make a new weight calibration and make sure that the Measurement Range is correct. If the DMT device enabler is not used the then also check the Force Rec Output values. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
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Page 29: Chapter 4 - The Multi Wire Myograph Unit
USING SOME KIND OF PERMANENT MARKER. STORE THE SUPPORTS IN THE PROVIDED PLASTIC CASE. NUMBERING THE SUPPORTS WILL SAVE TIME WHEN THE SUPPORTS ARE CHANGED AGAIN, LIMITING THE AMOUNT OF ADJUSTMENTS NEEDED AFTER EACH CHANGE. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk... - Page 30 4. Loosen screw D to move the jaw to the right or left on the transducer side. Figure 4.1 Myograph unit – screws for changing supports and coarse adjustment of the jaws Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 31 Jaws from side view Figure 4.3 Illustrations of properly aligned jaws (depicted on the far left) and incorrectly aligned jaws (depicted in the middle and far right). Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 32 Pins from side view Figure 4.5 Illustrations of properly aligned pins (depicted on the far left) and incorrectly aligned pins (depicted in the middle and far right). Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
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Page 33: Calibration Of The Force Transducer
4.2 CALIBRATION OF THE FORCE TRANSDUCER As a part of the general maintenance of the myograph, DMT recommends that the myograph is weight - calibrated at least once a month. The myograph should also be weight-calibrated every time the interface has been moved. - Page 34 CALIBRATION sub-menu is initiated, which is described in detail in Chapter 3. Figure 4.7 – Illustration of the proper placement for the balance transducer arm calibration Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
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Page 35: Checking The Force Transducer
AND the transducer cannot be recalibrated, the force transducer is broken and needs to be replaced. If any other problems related to the force transducer are encountered, please contact DMT for adv ice or further instructions. -
Page 36: Force Transducer Replacement
Figure 4.10 The 2 screws that secure the transducer house to the chamber Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk... -
Page 37: Myograph Maintenance
DESCRIBED IN CHAPTER 3 AND 4.2. 4.5 MYOGRAPH MAINTENANCE The Multi Wire Myograph System Model 620M is a very delicate and sophisticated piece of research equipment. DMT recommends that the following sections are read carefully and that the instructions are followed at all times. - Page 38 Figure 4.5 The areas where linear slide grease may be applied for smooth micropositioner movement Cleaning the myograph DMT strongly recommends that the myograph chambers and surrounding areas are cleaned after each experiment WARNING: GREASE FOR LINEAR SLIDES IS TOXIC FOR THE MOUNTED TISSUE. AVOID ANY CONTACT WITH THE MOUNTED TISSUE.
- Page 39 9. Wash the myograph chamber and supports several times with double distilled water. 10. Incubate the myograph chamber with 96% ethanol for 10 minutes while continuing the mechanical cleaning with a cotton-tip applicator. Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
- Page 40 CLEANING OF ALL SUPPORT SURFACES. NEVER SOAK THE SUPPORTS IN ANYTHING STRONGER THAN 8% ACETIC ACID FOR EXTENDED PERIODS OF TIME (I.E. SEVERAL HOURS OR OVERNIGHT) Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
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Page 41: Appendix 1 - System Specifications
±0.2 °C Heating TRANSDUCER: Output reading mN or g Range ±200/±400/±800/±1600mN Resolution 0.01mN Force calibration OUTPUT: Data communication USB 2.0 Analogue output channels Analogue output range ±2.5V Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk... - Page 42 Danish Myo Technology A/S | Tel.: +45 87411100 | Email: support@dmt.dk | Web: www.dmt.dk...
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