Teledyne ACCQPrep HP150 Installation Manual
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Teledyne ACCQPrep HP150 Installation Manual

Teledyne ACCQPrep HP150 Installation Manual

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ACCQPrep
Installation Guide
1.1 Overview
1.2 Safety
Information contained herein is classified as EAR99 under the
U.S. Export Administration Regulations. Export, reexport or
diversion contrary to U.S. law is prohibited.
HP150
®

Section 1 Safety Warnings

This Installation Guide provides:
• Safety information
• Unpacking instructions
• Installation instructions, including connections with
Windows and iOS operating systems
• Certification and warranty information
Before installing, operating, or maintaining this equipment, it is
imperative that all hazards and preventive measures are fully
understood. While specific hazards may vary according to
location and application, heed the following general warnings:
Liquids associated with this instrument may be classified
as carcinogenic, biohazard, flammable, or radioactive.
Should these liquids be used, it is highly recommended that
this application be accomplished in an isolated
environment designed for these types of materials in
accordance with federal, state, and local regulatory laws,
and in compliance with your company's chemical/hygiene
plan in the event of a spill.
Avoid hazardous practices! If you use this instrument in
any way not specified in this manual, the protection
provided by the instrument may be impaired.
If you are using flammable solvents or chemicals with this
system, vapor concentration levels may exceed the
maximum exposure levels as recommended by OSHA
Guide 1910.1000. To reduce those levels to a safe exposure,
Teledyne ISCO recommends that you place the system in
a laboratory hood designed for the purpose of ventilation.
This hood should be constructed and operated in
accordance with federal state and local regulations. In the
event of a solvent or chemical spill, your organization
should have a plan to deal with these mishaps. In all cases,
use good laboratory practices and standard safety
procedures.
Rev. E, December 6, 2023
WARNING
WARNING
WARNING
69-5233-800
1-1

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Summary of Contents for Teledyne ACCQPrep HP150

  • Page 1: Section 1 Safety Warnings

    OSHA Guide 1910.1000. To reduce those levels to a safe exposure, Teledyne ISCO recommends that you place the system in a laboratory hood designed for the purpose of ventilation. This hood should be constructed and operated in accordance with federal state and local regulations.
  • Page 2 ® ACCQPrep HP150 Installation Guide Section 1 Safety Warnings 1.3 Hazard Severity Levels This manual applies three Hazard Severity Levels to the safety alerts. These are described in the sample alerts below. CAUTION Cautions identify a potential hazard which, if not avoided, may result in minor or moderate injury.
  • Page 3 Esta señal alerta sobre la presencia de alto voltaje en el interior del producto. 1.5 Safety Components The power cord is a safety disconnect for the ACCQPrep HP150 (Figure 1-1). To remove power from the ACCQPrep, remove the power cord by pulling it straight out from the power inlet connector.
  • Page 4 ® ACCQPrep HP150 Installation Guide Section 1 Safety Warnings EAR99 Technology Subject to Restrictions Contained on the Cover Page...

  • Page 5: Section 2 Installation

    ACCQPrep HP150 Section 2 Installation 2.1 ACCQPrep The following section will cover the installation and connection of the pump and will review the priming steps. Installation Note When the system ships, it will ship in two boxes to mini- mize the weight. One box will contain the unit, and the other box will have the pump and assorted accessories.
  • Page 6 ACCQPrep HP150 Section 2 Installation 2. Push the arm to the left or right to remove the spacer. 3. Store the bracket and spacer. These parts should be rein- stalled if the system must be shipped again. When you are ready to place the system, be sure it is situated in such a way that the power cord is accessible for disconnection in case of malfunction.
  • Page 7 ACCQPrep HP150 Section 2 Installation Figure 2-3 Pump compartment with electrical connection shown 4. When the pump assembly is most of the way in the pump compartment, connect the electrical connections on each side of the pump. First, connect the black connection on the left side.
  • Page 8 ACCQPrep HP150 Section 2 Installation 5. Once the electrical connections are complete, push the pump assembly the rest of the way into the pump compart- ment until the holes in the front of the pump assembly are aligned with the corresponding holes in the case bottom.
  • Page 9 ACCQPrep HP150 Section 2 Installation inlet solvent line to the left side 3. Connect the shorter FEP pump’s PEEK tee. Slide the spring down the tube and place the open side of the wrench (included) over the tub- ing and FEP fitting and make small turns as to not bend the tubing (Figure 2-8).
  • Page 10 Section 2 Installation 2.2.4 ACCQPrep Equipped with Optional ELSD Note Teledyne ISCO recommends >99% pure nitrogen from a source that can deliver 2.5 SLPM at 60 to 70 psi. Figure 2-9 Location of ELSD gas port (Nitrogen input line circled) The ACCQPrep ELSD system has several additional connections.
  • Page 11 ACCQPrep HP150 Section 2 Installation Figure 2-10 Back of ACCQprep with optional ELSD 2. ELSD operation requires a carrier gas. To connect the car- rier gas to the ACCQPrep: · Locate the 1/8 inch O.D. FEP tubing (PN:023-0503-02) from the accessory package.
  • Page 12 ACCQPrep HP150 Section 2 Installation P-Trap drain vent assembly drain line Figure 2-11 Keep liquid level lower than the top of the system Route the end of the P-trap drain tube to a suitable waste fluid collection container. Please be aware that as the tub- ing is lowered, several mL of isopropyl alcohol will run out of the tubing.
  • Page 13 Section 2 Installation CAUTION Discontinue use of the ACCQPrep if liquid is present at the pump drain. Contact Teledyne ISCO technical service for assistance with correcting the leak. The system will require priming with the desired solvents. To 2.2.5 Priming Steps prime the system: 1.
  • Page 14 ACCQPrep HP150 Section 2 Installation Note The system will prime both pumps simultaneously. There is no method to prime A and B individually. 2.3 Installing the Manual sample injection can be performed with the system even when equipped with an AutoInjector or AutoSampler.
  • Page 15 ACCQPrep HP150 Section 2 Installation To install the AutoSampler: 1. Carefully unpack the Z-Drive and remove the packing materials from the Y-axis arm (Figure 2-15). Figure 2-15 AutoSampler Module and Z-Drive 2. Find the Y-axis carriage on the arm of the AutoSampler Module.
  • Page 16 ACCQPrep HP150 Section 2 Installation Figure 2-17 Z-Drive secured to the Y-axis carriage 5. Rotate the Z-Drive rotor back and forth to ensure that the Z-Drive moved up and down freely (Figure 2-18). Note If the rotor does not move freely, check that the other cables are not interfering with the movement of the green probe cable.
  • Page 17 ACCQPrep HP150 Section 2 Installation Overflow drain Wash station Waste line Wash station supply line Figure 2-19 AutoSampler Module wash well with tubing attached (top tube is waste to port #2 while the bottom tube is supply port #3) 7. The wash well comes pre-plumbed to the peristaltic pump ports.
  • Page 18 ACCQPrep HP150 Section 2 Installation 10. Connect the black tube labeled "XX" from the tee on the back of the AutoSampler to the attached 4-port cross on back of the ACCQPrep. (Figure 2-21). Figure 2-21 AutoSampler waste connection point on...
  • Page 19 AutoSampler”. The length of this tubing is critical to con- trol the delay volume for fraction collection on the AutoSampler. Use the length of tubing provided with your AutoSampler or with the appropriate Teledyne ISCO tub- ing replacement kit. Auto...
  • Page 20 ACCQPrep HP150 Section 2 Installation Autosampler Module should already be pre-installed. If not, make the connection as shown in Figure 2-23. Note The power supply accompanying the AutoSampler will not be used; instead, the AutoSampler will receive its power supply through the ACCQPrep.
  • Page 21 ACCQPrep HP150 Section 2 Installation bracket. This ensures that the probe tubing will not be obstructed by the other end of the probe attachment (Figure 2-26). Probe tubing Figure 2-26 Probe tubing affixed to the side of the ACCQPrep 4. Install the included 18 mm test tube in the receptacle at the right rear corner of the AutoSampler platform just to the left of the wash well (Figure 2-27).
  • Page 22 ACCQPrep HP150 Section 2 Installation Figure 2-28 On switch for the AutoSampler 2. Turn O the power to the ACCQPrep. A red light on the AutoSampler will light up, the arm will move to the H position, and then the light will turn blue (Figure 2-29).

  • Page 23: Column Installation

    ACCQPrep HP150 Section 2 Installation 3. Ensure the wash solvent is pumped into the center portion of the wash station and, when full, the fluid drains into the overflow drain (Figure 2-31). Overflow drain Wash station Waste line Wash station...
  • Page 24 ACCQPrep HP150 Section 2 Installation Figure 2-32 Column Mounting Parts To install a column onto the ACCQPrep: 1. Select the proper sized column mount and simply slip the mount into one of the two column mounting tracks on the side of the ACCQPrep (Figure 2-33).
  • Page 25 2.5.2 Column Configuration methods for individual columns. This allows easy selection of default methods with a single button press. This process is described in the ACCQPrep HP150 Operation Guide, Section 2.4.1. The ACCQPrep’s efficiency can be enhanced by the addition of 2.5.3 Column Select Valve...

  • Page 26: Installation Troubleshooting

    ACCQPrep HP150 Section 2 Installation Note Mixed reverse phase and normal phase solvents may require manual flushing of the pumping system between separations. • If the CSV-4 was ordered with the instrument, it will arrive installed in the ACCQPrep. • If you decide to add this capability at a later time, this...
  • Page 27 ACCQPrep HP150 Section 2 Installation AutoSampler control not visible on the ACCQPrep software – The ACCQPrep only loads the AutoSampler control features if it can detect the presence of the AutoSampler. Check the AutoSampler pilot light (upper right corner of AutoSampler) to determine the next step.
  • Page 28 Installation Qualification For ACCQPrep HP150 The following tasks are to be performed upon installation of the Teledyne ISCO ACCQPrep HP150. The following procedures should be performed in accordance with ACCQPrep HP150 Installation Guide (#69-5233-800) Description of task Yes No Comment...
  • Page 29 Operation Qualification For ACCQPrep HP150 The following tasks are to be performed after the installation of the ACCQPrep HP150 and all accessories: Description of task Comment Does the system power up? Does the fraction arm go to the “left rear” position?
  • Page 30 ACCQPrep HP150 Section 2 Installation 2-26 EAR99 Technology Subject to Restrictions Contained on the Cover Page...

  • Page 31: Product Model

    Compliance Statements DECLARATION CONFORMITY We the manufacturer: Manufacturer’s Name: Teledyne ISCO Manufacturer’s Address: 4700 Superior Street, Lincoln, NE 68504 Declare, under our sole responsibility that the following equipment: Product Model: ACCQPrep HP 125 ACCQPrep HP 150 Object of Declaration: Preparative High Performance Liquid Chromatography (Preparative HPLC) System.
  • Page 32 4700 Superior Street, Lincoln, NE 68504 Declare, under our sole responsibility that the following equipment: Product Model: ACCQPrep HP125 ACCQPrep HP150 Object of Declaration: Preparative High Performance Liquid Chromatography (Preparative HPLC) System. Is designed and manufactured in compliance with the following applicable Directives and Standards:...
  • Page 33 4700 Superior Street, Lincoln, NE 68504 Declare, under our sole responsibility that the following equipment: Product Model: ACCQPrep HP125 ACCQPrep HP150 Object of Declaration: Preparative High Performance Liquid Chromatography (Preparative HPLC) System. Is designed and manufactured in compliance with the following applicable Regulations and Standards:...
  • Page 34 Name and amount of Hazardous Substances or Elements in the product Hazardous Substances or Elements Component Name (Pb) (Hg) (Cd) (Cr(VI)) (PBB) (PBDE) LCD Display Circuit boards Wiring Internal Cables Line Cord Stepper Motor Deuterium lamp Valve Body Name and amount of Hazardous Substances or Elements in the product O: Represent the concentration of the hazardous substance in this component’s any homogeneous pieces is lower than the ST/ standard limitation.

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