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Related Manuals for KERN OBN 132
Summary of Contents for KERN OBN 132
- Page 1 KERN & Sohn GmbH Ziegelei 1 Tel: +49-[0]7433- 9933-0 D-72336 Balingen Fax: +49-[0]7433-9933-149 E-mail: info@kern-sohn.com Internet: www.kern-sohn.com User instructions Transmitted light laboratory microscope KERN OBN-13 OBN 132, OBN 135 Version 1.1 2025-02 OBN-13-BA-e-2511...
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Page 2: Table Of Contents
Assembly ..................16 Microscope head ........................17 Objectives ..........................17 Eyepieces ..........................17 Colour filter ( OBN 132 ) ......................17 Condenser ..........................17 Operation and function of the microscope ................18 (Pre-) focusing ........................19 Adjusting the interpupillary distance ................... 20 Dioptre adjusment ........................ - Page 3 11.5 Fluorescence reflected light unit ..................32 Troubleshooting ................. 43 Service ..................45 Power supply................45 14.1 Mains connection ........................45 Maintenance, servicing and disposal ........46 15.1 Maintenance and cleaning ..................... 46 15.2 Waste disposal ........................47 Further information ..............47 OBN-13-BA-e-2511...
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Page 4: Technical Data
1 Technical data Kern model OBN 132 OBN 135 Item number/type OBN 132 TOBN 135-A Dimensions (WxDxH) 306x200x395 mm Tubus Art Trinocular Optical system Infinity Revolving nosepiece screw-in positions Lens quality Infinity Plan Standard objectives 100x Eyepiece field width Illuminance... -
Page 5: Declaration Of Conformity
2 Declaration of conformity The current EC/EU Declaration of Conformity can be found online at: https://www.kern-sohn.com/shop/de/DOWNLOADS/ OBN-13-BA-e-2511... -
Page 6: Device Overview
3 Device overview Tube connectors with Eyepiece dioptre adjustment ring Camera adapter connection with adjusting screw Microscope head / tube Trinocular toggle rod Nosepiece Adjusting screw Objective Microscope head Object holder Specimen Setting wheel X - Y axis Condenser Object Aperture ON/OFF switch adjustment... - Page 7 Rear view OBN 132/ OBN 135 Carrying handle Coarse Fine adjustment knob Main switch Power Fuse connection Adjustment ring Torque Condenser Focus wheel OBN-13-BA-e-2511...
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Page 8: Before Use
4 Before use 4.1 General information The packaging must be opened carefully to prevent the accessories inside from falling to the floor and breaking. In general, a microscope should always be handled with great care, as it is a sensitive precision instrument. -
Page 9: Intended Use
Symbols in warning notices: Icon Meaning Warning signs warn you of dangers that could lead to Warnings injury. The symbol indicates the type of danger. Indicates general hazards or a danger point Warning of electrical voltage Warning of explosive substances Warning of optical radiation Icon Meaning... -
Page 10: Notes On The Electrical System
• Improper set-up or electrical installation • Improper assembly or electrical installation 5.5 Notes on the electrical system Before connecting the device to the mains, you must ensure that you are using the correct input voltage. The information for selecting the correct mains cable can be found on the device, on the back of the product directly above the connection socket. -
Page 11: Storage And Transportation
5.6 Storage and transportation Avoid exposing the device to direct sunlight, high or low temperatures, shocks, dust and high humidity. The suitable temperature range is 0 - 40 °C and a relative humidity of 85% should not be exceeded. The appliance should always be placed on a firm, smooth and horizontal surface. When the microscope is not in use, it is best to cover it with the dust cover supplied. -
Page 12: Basic Warnings And Safety Instructions
6 Basic warnings and safety instructions 6.1 Observe the notes in the operating instructions Read the operating instructions carefully before commissioning/using the device, even if you already have experience with KERN devices. Always keep the instructions in the immediate vicinity of the appliance Staff training The appliance may only be used by persons who have read and understood the operating instructions, in particular the chapter on safety. - Page 13 ● Never look into the eyepieces when the beam path is open (using the control lever for illumination) and an empty filter position is selected on the FL module. There is an acute risk of blindness here. ⚠ WARNING Risk of injury due to electric shock! ●...
- Page 14 ⚠ WARNING Never touch the glass housing of the built-in HBO replacement lamp with bare hands. Contamination increases the risk of explosion during operation. The lamp must be cleaned if it becomes contaminated. ● An HBO lamp has a certain service life. The closer it is brought to its limit, the greater the risk of the lamp exploding and releasing toxic mercury vapor.
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Page 15: Unpacking And Commissioning
On receipt of the device, please check in advance whether there is any damage caused during transportation and whether the outer packaging, the housing, other parts or even the device itself are damaged. If any damage is apparent, please notify KERN & Sohn GmbH immediately. OBN-13-BA-e-2511... -
Page 16: Assembly
8 Assembly OBN-13-BA-e-2511... -
Page 17: Microscope Head
Care should always be taken to ensure that the lenses are not touched with bare fingers and that no dust enters the openings. 8.4 Colour filter ( OBN 132 ) A blue colour filter can simply be placed in the ring holder of the field lens. -
Page 18: Operation And Function Of The Microscope
8.6 Operation and function of the microscope When the microscope is ready for use after assembly, it must first be connected to the mains via the mains cable. Only insert the mains plug into a suitable socket. Ensure that the mains cable is laid correctly. The following sections describe all the important functions that are useful for operating the device. -
Page 19: Pre-) Focusing
8.7 (Pre-) focusing When you are observing an object, you must have the correct distance to the objective to achieve a sharp image. In order to find this distance at the beginning (without other default settings of the microscope) place the objective with the lowest magnification in the beam path, look through the right eyepiece with the right eye and turn it slowly using the coarse adjustment knob (see illustration). -
Page 20: Adjusting The Interpupillary Distance
8.8 Adjusting the interpupillary distance With binocular viewing, the interpupillary distance must be adjusted accurately for each user, in order to achieve a clear image of the object. While you are looking through the eyepieces, use your hands to hold the righthand and lefthand tube housing firmly. -
Page 21: Adjusting The Magnification
8.10 Adjusting the magnification After prefocussing has been carried out using the objective with the lowest magnification (see section 5.2), you can then adjust the overall magnification using the nosepiece, as necessary. By turning the nosepiece you can bring any one of the four other objectives into the beam path. -
Page 22: Adjusting The Koehler Illumination
If, as with the devices in the KERN OBN-13 series, the lighting can be set in accordance with Koehler, the result is homogenous illumination of the slide and avoidance of disruptive stray light. - Page 23 4. Use the centring screws of the condenser holder to bring the image from the field diaphragm into the centre of the field of view. 5. Open the field diaphragm until it just disappears from the field of view. If necessary, slightly re-center with the centering screws of the condenser carrier.
- Page 24 If another magnification is selected afterwards, then the Koehler illumination does not have to be reset from scratch, only the aperture diaphragm and field diaphragm need to be adjusted as required. As a result you can always check whether the condenser needs to be re-centred OBN-13-BA-e-2511...
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Page 25: Using The Eyecups
8.12 Using the eyecups The eye cups supplied with the microscope can basically be used at all times, as they screen out intrusive light, which is reflected from light sources from the environment onto the eyepiece and the result is a better image quality. But primarily, if eyepieces with a high eye point (particularly suitable for those who wear glasses) are used, then it may also be useful for users who don’t wear glasses, to fit the eye cups to the eyepieces. -
Page 26: Use Oil Immersion Lenses
8.13 Use oil immersion lenses The 100x objectives of the OBN-13 series are objectives which can be used with oil immersion (they are always marked with the word “OIL”). Using these generates a particularly high resolution for microscopic images. To use oil immersion correctly, please follow these steps. 1. -
Page 27: Changing The Bulb
In most cases, problems with the lighting would therefore be caused by defects in the electrical system. In such a case, our Technical Service can help. 10 Changing the fuse (OBN 132) The fuse housing is located at the rear of the microscope below the mains plug connection. -
Page 28: Use Of Optional Accessories
11 Use of optional accessories 11.1 Polarization unit The polarization unit consists of two parts: Polarizer and analyzer. Both parts consist of a round glass plate with an outer retaining ring. To attach the analyzer, a slider with holder is attached to the front of the microscope directly above the nosepiece (see illustration on page 16), where the analyzer can be easily inserted (see illustration on the left). -
Page 29: Camera Connection
11.2 Camera connection Due to the trinocular tube, which is a standard fitting for the whole OBN-13 series, it is possible to connect microscope cameras to the device, in order to digitally record images or sequences of images of an object being observed. After the plastic cover has been removed from the camera adapter connector on the top of the microscope head, then a suitable adapter must be fitted. -
Page 30: Phase Contrast Units
11.4 Phase contrast units Basically, there are two different ways to enable the use of phase contrast methods. A simple and a more extensive method. 1. Simple phase contrast unit This consists of a simple PH condenser, a PH objective with a specific magnification (10x, 20x, 40x or 100x), a PH slider, which is adapted to the lens being used, a centring telescope and two green filters. - Page 31 With the surface marked “TOP” facing up, the PH slider is pushed upwards into the insertion point on the PH condenser until it clicks into the first position. At this first position, the phase ring of the PH slider is in the beam path. If the aperture setting on the PH condenser is set to “PH”, then the phase-contrast application can begin.
- Page 32 The adjustment procedure is as follows. a. First adjust the microscope using bright field mode. b. Move the hub to the required PH position (e.g. “10x”). c. Open the aperture diaphragm on the condenser to its maximum (slider to the left).
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Page 33: Fluorescence Reflected Light Unit
Fluorescence reflected light unit There are samples, which can be excited by light beams and thereby show emissions, which have different wave lengths than the previous excitation beams. The wave length of the emission is always bigger than the wave length of the excitation (Stokes shift). - Page 34 Overview of the wavelengths for excitation and emission per excitation filter Blue line: Wavelength excitation Green line: wavelength emission OBN-13-BA-e-2511...
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- Page 37 Nomenclature (components) Protective plate HBO/LED lamp housing Cover for FL module Power connection Fixing Allen screw for microscope head Screw for attaching the protective plate Main body Connection point for microscope housing Connection point for microscope head Allen wrench Fixing screw for lamp housing OBN-13-BA-e-2511...
- Page 38 Nomenclature (control elements) Control wheel for FL module Condenser control Centring device for field diaphragm Screw for cover of lamp housing Control lever for field diaphragm Centring screw for lamp (vertical) Centring device for aperture diaphragm Centring screw for lamp (horizontal) Control lever for aperture diaphragm Control lever for illumination OBN-13-BA-e-2511...
- Page 39 Nomenclature (power supply unit for HBO lamp) Operating time Ampere meter Main switch Basic data Four different fluorescence incident light units are optionally available for the OBN-13 series microscopes, which have the following specifications. Article number Illumination Excitation Centering Input voltage Power filter...
- Page 40 Assembly The following steps must be carried out in order to correctly assemble the fluorescence incident light unit or mount it on the microscope housing. 1. If the microscope is already set up without the FL unit, the microscope head must first be removed.
- Page 41 Control elements for illumination The following control elements for the illumination play a role within the fluorescence microscopy: Field diaphragm, aperture diaphragm, condenser: Optimisation of the contrast and the light field. Control lever for illumination: When located at the medium position there is a filter, which softens the lighting, so that samples, which are not stable enough for the full illuminating power, can be observed as well.
- Page 42 Lamp centering (HBO) As the operating time of the HBO lamp progresses, it is possible that its holder will become deformed due to the strong heat development and thus move from its center. As this means that the field of view is no longer evenly illuminated, this situation must be corrected as follows if it occurs.
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Page 43: Troubleshooting
12 Troubleshooting Problem Possible causes Mains plug not plugged in correctly No power available at the socket Lamp does not burn Lamp defective Fuse defective Lamp burns out immediately The prescribed lamp or fuse is not used Aperture diaphragm and/or field diaphragm are not open wide enough The beam path selector slider is set to Field of vision is dark... - Page 44 Problem Possible causes Aperture diaphragm is not open wide enough Condenser is lowered too far The objective does not belong to this microscope Blurred details The front lens of the lens is dirty An immersion lens is used without Bad picture immersion oil Poor contrast The immersion oil contains air bubbles...
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Page 45: Service
If, despite studying these operating instructions, you still have questions about commissioning or operation, or if, contrary to expectations, a problem should occur, please contact your specialist dealer. The device may only be opened by trained service technicians authorized by KERN. 14 Power supply 14.1 Mains connection The microscope may only be connected to the mains if the information on the microscope ( sticker ) and the local mains voltage are identical. -
Page 46: Maintenance, Servicing And Disposal
• Special cleaner for optical lenses • Special optical cleaning cloths • Bellows • Brush If handled correctly and checked regularly, the microscope will function smoothly for many years. Should a repair nevertheless be necessary, please contact your KERN dealer or our Technical Service. OBN-13-BA-e-2511... -
Page 47: Waste Disposal
15.2 Waste disposal Old appliances and accessories must not be disposed of with household waste. The operator must dispose of the packaging and the device at the place of use in accordance with the applicable national or regional legislation The device consists of various components and materials, such as •...
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