Leica Aperio GT 450 DX Quick Reference Manual
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Leica Aperio GT 450 DX Quick Reference Manual

Leica Aperio GT 450 DX Quick Reference Manual

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Aperio GT 450 DX
Quick Reference Guide
MAN-0477, Revision C | March 2025

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Summary of Contents for Leica Aperio GT 450 DX

  • Page 1 Aperio GT 450 DX Quick Reference Guide MAN-0477, Revision C | March 2025...
  • Page 2 Aperio GT 450 DX Quick Reference Guide This manual applies to the Aperio GT 450 DX Controller, Aperio GT 450 DX Console, and Aperio GT 450 DX SAM DX versions 1.1 and later. Copyright Notice Ì Copyright © 2022-2025 Leica Biosystems Imaging, Inc. All Rights Reserved. LEICA and the Leica logo are registered trademarks of Leica Microsystems IR GmbH.

  • Page 3: Table Of Contents

    Aperio GT 450 DX Quick Reference Guide Contents 1. INTRODUCTION ........5 Load Racks in the Carousel ......14 Unload Racks from the Carousel Help Videos ............ 5 ......15 Priority Scanning .......... 16 User Guides ............ 5 Scan Status ..........17 Scanner Overview ...........
  • Page 4 Aperio GT 450 DX Quick Reference Guide Shut Down the Scanner ........ 24 5. TROUBLESHOOTING ......25 Additional Resources ........25 Serial Number and Firmware Version ..25 Open and Close the Scanner Cover ....26 Return Pusher to Safe Position ....27 Safe Restart After an Error ......

  • Page 5: Introduction

    Aperio GT 450 DX Quick Reference Guide Introduction This guide provides basic steps for using the Aperio GT 450 DX digital slide scanner. See below for additional resources. Please watch the videos (accessible from the touchscreen interface) before working with the scanner.

  • Page 6: Scanner Overview

    Aperio GT 450 DX Quick Reference Guide Scanner Overview Front Back Fan fi lter Touchscreen interface Carousel Status lights (6) On/off switch Rack loading area (front 6) Power / network connections 6 | Introduction...

  • Page 7: Touchscreen Interface Overview

    Aperio GT 450 DX Quick Reference Guide Touchscreen Interface Overview The example below shows the Home screen. Help videos and guides Log in to the scanner Maintenance area Rack view Scanner statistics Slide view Home screen Rack order (carousel view)

  • Page 8: Scanning Workflow

    Aperio GT 450 DX Quick Reference Guide Scanning Workflow This workflow shows the scanning process for one rack of slides. For step-by-step instructions, go to “Scan Slides” on page 13. Slides loaded Rack rotated Scanning starts Scan and Image QC complete...

  • Page 9: Carousel Rotation

    Aperio GT 450 DX Quick Reference Guide Carousel Rotation The carousel automatically rotates after you load racks: Safety light curtain An invisible safety light 10-second timer before 1. Racks rotated to scan curtain detects objects Racks loaded carousel rotates: area in order loaded...

  • Page 10: Key Concepts

    Image QC were successful for all slides in the rack. Slide calibration Each slide is calibrated when it’s scanned. Continuous The Aperio GT 450 DX is a continuous loading scanner. You can continue to load new racks and loading unload completed racks without interruption. Automatic The carousel rotates the racks automatically.

  • Page 11: Icon Glossary

    Aperio GT 450 DX Quick Reference Guide Icon Glossary The following icons appear on the touchscreen interface. Scan completed – In Rack View, indicates the Carousel – Carousel view (Home screen). slide scanned successfully, passed image QC, and Stats – Scanner statistics. (See page 18.) that the image was saved to the specified image location.

  • Page 12: Slide Preparation Checklists

    Aperio GT 450 DX Quick Reference Guide Slide Preparation Checklists Slides Slide Labels Slides stained according to manufacturer’s instructions. … Only one label per slide. … Slides are clean. Wipe with clean lint-free cloth (no chemical … Labels do not extend past edges of the slide.

  • Page 13: Scan Slides

    Aperio GT 450 DX Quick Reference Guide Scan Slides This section contains instructions for scanning slides. Push the slides into the rack until they touch the back Load Slides in the Rack of the rack. Make sure the Leica logo and “SIDE UP”...

  • Page 14: Load Racks In The Carousel

    Aperio GT 450 DX Quick Reference Guide Load Racks in the Carousel Slides are scanned in the order loaded. Place the loaded rack into an open slot in the carousel in Continue loading racks as needed. the orientation shown on page 13.

  • Page 15: Unload Racks From The Carousel

    Aperio GT 450 DX Quick Reference Guide Unload Racks from the Carousel Remove the rack from the rack slot. Tap Rotate to rotate the rack to the loading area. To unload a rack that is not in the loading area: Tap the rack position.

  • Page 16: Priority Scanning

    Aperio GT 450 DX Quick Reference Guide Priority Scanning Use Priority to move one or more racks to the front of the Tap Priority. scan queue. You can apply the Priority option on up to three racks at a time.

  • Page 17: Scan Status

    Aperio GT 450 DX Quick Reference Guide Scan Status CAUTION: If you must remove a rack before all The rack statuses are: slides in the rack are scanned, note the rack and Empty Rack is empty and available for use.

  • Page 18: Scan Statistics

    Aperio GT 450 DX Quick Reference Guide Scan Statistics To view scan statistics, tap Stats. To change the time frame of the statistics, tap the buttons above the graph. 18 | Scan Slides...

  • Page 19: Rack View

    Aperio GT 450 DX Quick Reference Guide Rack View Tap the Rack View icon to show the scan status for each slide in the rack. Slide status colors correspond to the Legend: The slide is scanning The slide is waiting to scan The slide slot is empty.

  • Page 20: Slide View

    Aperio GT 450 DX Quick Reference Guide Slide View Rack Order Select the rack by tapping it on the touchscreen. Tap the Rack Order icon to show the scan order of the racks. Tap the Slide View icon to individual images for the selected rack.

  • Page 21: Rack Warnings

    Aperio GT 450 DX Quick Reference Guide Rack Warnings An orange rack position indicates a problem with the rack, or Slide issues: one or more slides in the rack. Orange warning Tap to select the rack position with the warning.

  • Page 22: Scan Entire Slide For Whole Rack

    Aperio GT 450 DX Quick Reference Guide Scan Entire Slide for Whole Rack The Scan Entire Slide option is selected, and the Settings icon appears on the rack position. This feature enables you to scan the full slide area for a rack of slides.

  • Page 23: Maintenance

    Aperio GT 450 DX Quick Reference Guide Maintenance Maintenance Checklists To maximize scanner performance and image quality, follow this recommended maintenance schedule. Daily (recommended) Six Month Yearly Restart the scanner. (See Clean the objective and Koehler Leica Biosystems Technical …...

  • Page 24: Restart The Scanner

    Aperio GT 450 DX Quick Reference Guide Restart the Scanner Shut Down the Scanner Restart the scanner once daily for maintenance. Use this procedure to safely turn off the scanner. From the touchscreen interface, tap Maintenance. CAUTION: Restarting the scanner with a slide on the stage may damage the slide.

  • Page 25: Troubleshooting

    Maintenance, then tap Maintenance Videos. Serial Number and Firmware Version You need the serial number and current software version if you call Leica Biosystems Technical Support. Tap Maintenance on the touchscreen to view the serial number, firmware version, and other system information.

  • Page 26: Open And Close The Scanner Cover

    Aperio GT 450 DX Quick Reference Guide Open and Close the Scanner Cover You need to open the cover to perform certain maintenance and troubleshooting tasks. For detailed instructions on how to open and close the scanner, see the user’s guide for the scanner.

  • Page 27: Return Pusher To Safe Position

    Aperio GT 450 DX Quick Reference Guide Return Pusher to Safe Position Follow these steps when the troubleshooting procedure asks you to return the pusher to the safe position. Align the pusher teeth with the slide stage groves: Slide the stage to the rear of the scanner: Hold the pusher in one of the areas circled in blue, Slide the pusher to the front of the scanner as shown.

  • Page 28: Safe Restart After An Error

    Aperio GT 450 DX Quick Reference Guide Safe Restart After an Error Some procedures ask you to restart the scanner. Restarting the scanner initializes the controller and puts the stage and autoloader in their home positions. Before restarting the scanner, you need to ensure there is no slide on the stage.

  • Page 29: Errors And Solutions

    Error Code 1000 Internal Error Open the cover and access the interior (see page 26). Take photos of any obstructions for Leica Biosystems Technical Services. Check if there is a slide on the stage or partially on the stage. CAUTION: Restarting the...
  • Page 30 Aperio GT 450 DX Quick Reference Guide Error Code 1001 Scanner Cannot Initialize Open the cover and access the interior (see page 26). Check if there is a slide on the stage or partially on the stage. CAUTION: Restarting the...
  • Page 31 Check the rack loading area and pinch points for any unexpected object: unexpected object: Ensure Leica logo faces up and out of the racks, and that If there are no obstructions and carousel still does not racks are inserted fully in the carousel.
  • Page 32 33 and follow the steps for the specific slide error. Technical Services. If the rack is supported and undamaged, reinsert it into the carousel for scanning. If the scanner still cannot process the rack, contact Leica Biosystems Technical Services. 32 | Troubleshooting...
  • Page 33 Aperio GT 450 DX Quick Reference Guide Slide Error Messages for Error Code 1006 After identifying which slide has the error, follow the appropriate steps below. You can temporarily hide some error messages by tapping the x in the top-right corner.
  • Page 34 Aperio GT 450 DX Quick Reference Guide Error Message What to do No Macro Focus Check the slides and rack are inserted correctly. See page 13. Check that the slide tray is clean. Check the stain quality. Check that the slide and coverslip meet the requirements described in the scanner Specifications document.
  • Page 35 Aperio GT 450 DX Quick Reference Guide Error Message What to do Image Transfer Error message appears on some slides: Error message appears on all slides: Error - Pending The system often resolves the issue without The Lab Administrator should: Retry intervention.
  • Page 36 Aperio GT 450 DX Quick Reference Guide Error Message What to do Aborted Error message appears on some slides: Error message appears on all slides: Check for damage or common slide Remove any completed slide racks from the preparation issues. (See page 12.) carousel.
  • Page 37 Aperio GT 450 DX Quick Reference Guide Error Code 1007 Internal storage full. Cannot send images to DICOM converter The Lab Administrator should perform these steps. Ensure the LAN cables are connected at the scanner LAN port and at the SAM server.
  • Page 38 Check for obstruction near the slide stage, rack, and pusher. If possible, carefully remove the slide that is causing the obstruction, and continue to step 8. If you cannot remove the obstruction or if the issue persists, call Leica Biosystems Technical Services. 38 | Troubleshooting...
  • Page 39 Aperio GT 450 DX Quick Reference Guide WARNING: Do not attempt to recover broken slides. Call Leica Biosystems Technical Services. Check the slide for preparation issues, such as over-hanging coverslips and label issues. If there are no slide preparation issues, re-insert the slide in an available rack for scanning.
  • Page 40 Slide handling error at rack gripper, lift, or carousel Open the scanner cover to access the interior. See page 26. Take photos of the obstruction for Leica Biosystems Technical Services. Check if there is a slide on the stage or partially on the stage.
  • Page 41 Aperio GT 450 DX Quick Reference Guide Error Code 9000 Scanner cover is open. Slide the cover to the closed position, making sure the cover aligns with rear latches and clicks closed: 41 | Troubleshooting...

  • Page 42: Broken Slides Inside The Scanner

    Broken Slides Inside the Scanner WARNING: Do not attempt to recover broken slides. Call Leica Biosystems Technical Services. Take photos of the location of the damage. Leica Biosystems Technical Services may request the photos when providing assistance. 42 | Troubleshooting...

  • Page 43: Index

    Aperio GT 450 DX Quick Reference Guide Index load slides 13 Auto-Image QC 10 error codes and resolutions 29 error, rack 17 macro focus error 34 macro image 20 barcode error 33 magnification 10 barcodes 12 firmware version, locate 25...
  • Page 44 Aperio GT 450 DX Quick Reference Guide preparation, slide 12 slide view 20 priority scan 16 software version, locate 25 scan entire slide single slide 20 pusher, position 27 statistics 18 whole rack 22 status scan error 17 legend 17...
  • Page 45 Aperio GT 450 DX Quick Reference Guide warnings, rack 21 workflow, scan 8 Index | 45...
  • Page 46 Aperio GT 450 DX Quick Reference Guide 46 | Index...

  • Page 47: Revision Record

    Ì Serious Incidents Reporting - Any serious incident that has occurred in relation to the Aperio GT 450 DX shall be reported to the manufacturer and the competent authority of the member state in which the user and/or the patient is established.
  • Page 48 For Aperio recommendations on protecting your workstations and servers, see the document Aperio GT 450 DX IT Manager and Lab Administrator Guide. If a suspected Aperio GT 450 DX cybersecurity vulnerability or incident is detected, contact Leica Biosystems Technical Services for assistance.
  • Page 50 LeicaBiosystems.com/Aperio Copyright © 2022-2025 Leica Biosystems Imaging, Inc. All Rights Reserved. LEICA and the Leica logo are registered trademarks of Leica Microsystems IR GmbH. Printed in the U.S. MAN-0477 Revision C 03/2025...

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