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Kendro Sorvall Discovery 100 SE Operating Instructions Manual

Ultracentrifuge
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SORVALL
Discovery 100 SE
Discovery 90 SE
PN 45912-2
OPERATING
INSTRUCTIONS
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Summary of Contents for Kendro Sorvall Discovery 100 SE

  • Page 1 SORVALL ® Discovery 100 SE ™ Discovery 90 SE ™ OPERATING INSTRUCTIONS PN 45912-2...
  • Page 2 OPERATING INSTRUCTIONS SORVALL ® Discovery 100 SE ™ Discovery ™ 90 SE Ultracentrifuge Kendro Laboratory Products Newtown, Connecticut U.S.A. PN 45912-2 Issued June 2003...

  • Page 3: Safety Definitions

    Because failure to follow the recommendations set forth in this manual could produce personal injury or property damage, always follow the recommendations set forth herein. Kendro does not guarantee results and assumes no obligation for the performance of centrifuges or other products that are not used in accordance with the instructions provided.

  • Page 4: Important Safety Reminder

    Important Safety Reminder Certain potentially dangerous conditions are inherent to the use of all centrifuges. To ensure safe operation of this centrifuge, anyone using it should be aware of all safe practices and take all precautions described below and throughout these operating instructions. DANGER : •...
  • Page 5 • Supply power must be checked before the centrifuge is connected to power because the centrifuge can be damaged if connected to the wrong voltage. Kendro is not responsible for incorrect installation and warranty is void if an initial installation or electrical modification of the centrifuge is not performed by Kendro or a Kendro representative.
  • Page 6 Important Safety Reminder (continued) CAUTION : • Do not place fluids inside, on top of, or close to the centrifuge --- spillage can result in electrical or mechanical failure. • Always operate and maintain the centrifuge and all rotors as instructed in this manual and in the rotor manual(s).

  • Page 7: Safety Reminder

    Safety Reminder...

  • Page 8: Table Of Contents

    Table of contents 1. Description 1 --- 1 • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 1-1 General discriptions 1 --- 1 •...
  • Page 9 2-8 Features of the menu screen 2 --- 77 • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 2-8-1 Centrifuge scheduler 2 --- 77 •...

  • Page 11: Description

    1-1 General description 1. Description 1-1 General description The Discovery 100 SE and Discovery 90 SE Ultracentrifuges are designed and manufactured based on our long experience in the development of centrifuges; they are easy to use and highly reliable. Features include the following: Maximum speed of 100,000 rpm (802,006 x g) Control Panel is simple with easy key operation and easy-to-see liquid crystal screen.

  • Page 12: External View Of Ultracentrifuge

    1-1 General description 1-1-1 External view of ultracentrifuge The Discovery Series ultracentrifuges have the same external view, except for the model name printed on the front cover. The following is the external view of the Discovery 100 SE and the Discovery SE ultracentrifuges.

  • Page 13: Design

    1-2 Design 1-2 Design 1-2-1 Control panel The control panel for the Discovery 100 SE and Discovery 90 SE consists of a display panel and a keyboard. The display panel incorporates an easy-to-read liquid crystal display. The display panel (field display) displays running conditions and running status (this screen is called the Run Screen), along with features such as Programmed Run, Rotors List, and User Customizations Screens.
  • Page 14 1-2 Design [Functions of the display panel-keyed by item no. to fig. 1-2-1] Functions and actions Name and symbol Field display Displays various fields. The SPEED, TIME, and TEMP fields give the current status indicator in the top row and the setting indicator in the bottom row. (For setting, see Section 2-2-1.) SPEED •...
  • Page 15 1-2 Design [Functions of keyboard-keyed by item no. to fig. 1-2-2] Name and symbol Functions and actions START key Starts rotor rotation. If VACUUM is off, this key activates the vacuum pump and starts temperature control. STOP key Stops rotor rotation. VACUUM key Starts up the vacuum pump and activates air vent (As soon as vacuum pump is on, temperature control starts.) Air vent for vacuum chamber after a run cannot...

  • Page 16: Rotor Chamber

    1-2 Design 1-2-2 Rotor chamber The structure of the rotor chamber (vacuum chamber) is shown in Fig. 1-2-3. Handle Drive spindle Chamber door Chamber door seal Temperature sensor (measuring the Speed sensor temperature of the rotor) Overspeed detector Door lock system (detecting any instance exceeding the maximum allowable speed of the rotor)

  • Page 17: Safety Devices

    1-2 Design 1-2-3 Safety devices (1) Containment Should a rotor failure occur, the guard ring will contain the contents of the rotor inside the centrifuge (Fig. 1-2-3). (2) Imbalance detector If during operation the vibration of the rotor becomes excessive due to serious imbalance or improper bucket setting, the imbalance detector detects the situation and decelerates the rotor immediately.

  • Page 18: Rotor Adapter

    1-2 Design 1-2-4 Rotor adapter Overspeed Decal The overspeed decal located on the rotor base has alternating black and white bands. The number of bands corresponds to the maximum permitted speed of the rotor. (See Fig. 1-2-4.) Overspeed decal Standard rotor with overspeed decal Fig.
  • Page 19 1-2 Design To protect the overspeed decal, be sure to store the rotor on the rotor stand provided. (See Fig. 1-2-5.) Fig. 1-2-5 Rotor stand 1 --- 9...
  • Page 21 IMPORTANT Before operating the centrifuge , read and observe all safety information on the Important Safety Reminder pages in the front of this manual.
  • Page 22 IMPORTANT Before operating the centrifuge , read and observe all safety information on the Important Safety Reminder pages in the front of this manual.

  • Page 23: Operation

    2. Operation 2. Operation The Discovery 100 SE and Discovery 90 SE Ultacentrifuges are capable of operation in more than one mode to meet a wide range of applications. The outline of each available mode is given below: Outline Reference Section 2-3-1 “Normal operation”...
  • Page 24 2. Operation Outline Reference Calculates and displays the running time from the Section 2-5-5 “ setting”. set values of speed and setting Time Zonal operation is a mode of operation using a Section 2-3-2 “Zonal operation”. zonal rotor. Zonal operation Zonal speed Time 2 --- 2...

  • Page 25: Run Preparation

    2-1 Run preparation 2-1 Run preparation WARNING (1) Never use any materials capable of producing flammable or explosive vapors, or extreme exothermic reactions. (2) When using radioactive, toxic, or pathogenic materials, be aware of all characteristics of the materials and hazards associated with them in the event leakage occurs during centrifugation.

  • Page 26: Preparing Tubes/Bottles And Rotor

    2-1 Run preparation 2-1-2 Preparing tubes/bottles and rotor The Discovery 100 SE and Discovery 90 SE ultracentrifuges allow you to balance, by eye, tubes or bottles containing a sample solution and then centrifuge them. Make sure that the difference between meniscus levels of sample solution in tubes or bottles in within 5 mm (0.197 in) (See fig.

  • Page 27: Basic Operation

    (1) Do not operate the keyboard with a ball-point pen or a sharp object. (2) Do not continue to operate the centrifuge if abnormal sounds occur during operation. Immediately discontinue use of the centrifuge and contact Kendro Service. 2-2-1 Setting run conditions This section will first describe the screen for basic operation (the Run Screen) and the cursor keys.
  • Page 28 2-2 Basic operation [Cursor key] Pressing the cursor key will highlight the field where changes can be made. (This blinking/highlighted object is referred to as the cursor in this manual.) The screen setting field is in either of the following states depending on whether the cursor is there. (1) Determined input state: This is a normal state and the cursor does not appear.
  • Page 29 2-2 Basic operation (1) If you enter the wrong value, press CE key to return to the input wait state. NOTE If you have pressed ENTER key, press a cursor key, enter the device into an input wait state, then enter the correct value. (2) When setting two or more run conditions, you do not have to press ENTER key after each setting.
  • Page 30 2-2 Basic operation • How to set speed, running time, temperature, and other parameters Here are some examples and descriptions: Running time Setting item (SPEED) (TIME) Typical setting 100,000 rpm 2 hours 30 minutes Press cursor keys to enter the system into The system enters an input 2 hours 30 minutes an input wait state.
  • Page 31 2-2 Basic operation Temperature Acceleration Deceleration (TEMP) (ACCEL) (DECEL) 4.5 °C Enters an input wait state. Enters an input wait state. Enters an input wait state. The cursor blinks at one place. When one decimal place is not For free coast, press HOLD/FREE. required, you do not need to enter Display: F .

  • Page 32: Setting User Id Code

    2-2 Basic operation 2-2-2 Setting user ID code The USER ID CODE is a number to identify each user and can be set in up to 4 digits. When the user ID CODE is entered, the user record will be stored in the memory of the centrifuge and can be printed if a printout operation is done (optional).

  • Page 33: Using Rotor Life Management

    2-2 Basic operation 2-2-3 Using Rotor Life Management The rotor type and serial number may be set in rotor management to allow for rotor logging within the centrifuge. Whether the rotor type and serial number for a particular rotor have been set or not, it does not affect the normal operation of the ultracentrifuge.
  • Page 34 2-2 Basic operation Step Key operation Screen display and considerations Select “Running”. • The display switches back to the Run Screen and “T-8100-1234” is ENTER displayed in the message field. 2 --- 12...

  • Page 35: Basic Operating Procedure

    2-3 Basic operating procedure 2-3 Basic operating procedure There are two basic modes of operation, normal and zonal. The procedures for these two modes are shown in Fig. 2-3-1. Move the cursor to ZONAL in the Run Screen. START Zonal run Press 3 ENTER .
  • Page 36 IMPORTANT Before operating the centrifuge , read and observe all safety information on the Important Safety Reminder pages in the front of this manual.
  • Page 37 IMPORTANT Before operating the centrifuge , read and observe all safety information on the Important Safety Reminder pages in the front of this manual.

  • Page 38: Normal Operation

    2-3 Basic operating procedure 2-3-1 Normal operation Given below is a description of the operational procedure for a normal run. NOTE Before starting up this machine, carefully read the operation manual for your rotor and make sure that you Time have selected the correct type of tubes and entered the Fig.
  • Page 39 2-3 Basic operating procedure Step Operation and keystrokes Unit operation and considerations The specified centrifugation time >> The rotor decelerates and stops. elapses (time-out). Or press STOP key. STOP The rotor brakes or coasts to a stop. >> Beeps to indicate that the rotor has stopped. Press VACUUM key.

  • Page 40: Zonal Operation

    2-3 Basic operating procedure 2-3-2 Zonal operation Zonal operation is a mode of operation using a zonal rotor or continuous flow rotor for density gradient centrifugation with large amounts of sample or continuous sedimentation of particles from a large volume sample.
  • Page 41 2-3 Basic operating procedure Step Operation and keystrokes Unit operation and consideration • The characters On the Run Screen, move the cursor ZONAL are displayed in to ZONAL and enter the following by the message display field operating numeric keys to set the on the screen and the zonal operation mode.
  • Page 42 2-3 Basic operating procedure Step Operation and keystrokes Unit operation and consideration If you need to stop the run before the >> Rotor is decelerated to the zonal speed and then the buzzer sounds. set time elapses, press the STOP key. STOP Press the VACUUM key.

  • Page 43: Acceleration And Deceleration Rates

    2-4 Acceleration and deceleration rates 2-4 Acceleration and deceleration rates In order to meet various experimental protocols, the acceleration and deceleration rates can be adjusted between 0 to 500 rpm. The figure and table below show the relationship between ACCEL/DECEL code numbers selected and resulting approximate acceleration/deceleration times.
  • Page 44 2-4 Acceleration and deceleration rates Allowable maximum speed 500rpm 15 minutes 2 minutes 15 minutes 2 minutes Fig. 2-4-1 Operation with setting ACCEL = 0 and DECEL = 0 2 --- 21...

  • Page 45: How To Use The Function Field

    2-5 How to use the FUNCTION field 2-5 How to use the FUNCTION field This ultracentrifuge incorporates a number of features. These features are displayed and specified in the FUNCTION field. The FUNCTION field is extended as shown in the following figure. PROG •...

  • Page 46: Programmed Operation

    2-5-1 Programmed operation 2-5-1 Programmed operation This ultracentrifuge has a programmed operation feature that stores run conditions. Storing run conditions which you often use allows you to recall those conditions as often as you wish, thus saving time in setting. (The saved run conditions will remain in effect even after the power switch is turned off.) This centrifuge incorporates the program areas indicated below.
  • Page 47 2-5-1 Programmed operation (Basic operation of the programmed operation feature) Use cursor keys PROG • ENTER key select PROG RTC and • press ENTER key. Use cursor keys PROG ENTER key select PROG and If you know which memory press ENTER key. number stores the run condition.
  • Page 48 2-5-1 Programmed operation Continued Running Creating or Changing Deleting NOTE You cannot create, change, or delete a program while in running. Perform these operations while not in running. However, you can search the memory screen every time. 2 --- 25...
  • Page 49 2-5-1 Programmed operation 1. Programming procedure for run conditions (creating or changing) Shown below is the procedure for storing (creating) or changing an run condition. Step Key operation Screen display and considerations While in the Run Screen, use the cursor keys to move the cursor to PROG RTC and press ENTER •...
  • Page 50 2-5-1 Programmed operation Step Key operation Screen display and considerations • The message field in the Select “Creating or Changing.” bottom row switches to ENTER what is shown in the figure to the left. Enter a desired condition. • The display switches to For example, select "SPEED".
  • Page 51 2-5-1 Programmed operation Step Key operation Screen display and considerations (3) Select fixed angle rotor T-880. • The specifications of the T-880 are displayed. ENTER (4) Select "Yes". • The display switches to To select a desired rotor from the the Rotor Management "Rotor Management": (1') Select "Rotor Management".
  • Page 52 2-5-1 Programmed operation Step Key operation Screen display and considerations Enter values into SPEED (RCFmax or • When you finish entering RCFavg or ), TIME, TEMP, A run conditions for step 1, (ACCEL), and D (DECEL). step 2 enters into an input wait state.
  • Page 53 2-5-1 Programmed operation Step Key operation Screen display and considerations (3) Select “Inputting User Name”. (3) The display switches to the ID CODE input field. ENTER (4) Enter 4 digits of the ID CODE that (4) The display switches to the User Name check field. is already registered.
  • Page 54 2-5-1 Programmed operation Step Key operation Screen display and considerations (5) After entering the characters for (5) The display switches to the Remark check field. Remark, move the cursor to SET and press the ENTER key to register the character string. (6) Check the contents of Remark and select “Yes”.
  • Page 55 2-5-1 Programmed operation Step Key operation Screen display and considerations >> The fifth push on ESC key switches you back to the Run Screen (FUNCTION field). (1) If you make and store changes in a memory area that already stores run conditions, the NOTE previous conditions are replaced by the new conditions.
  • Page 56 2-5-1 Programmed operation 2. How to perform a programmed operation Shown below is how to perform a “programmed operation”, that is, how to call a stored set of run conditions and run this centrifuge accordingly. (a) If you know which memory unit number you need Step Key operation Screen display and considerations...
  • Page 57 2-5-1 Programmed operation Step Key operation Screen display and considerations Run the centrifuge under normal >> Run this machine according to 2-3-1 “Normal operation”. operation without making changes to >> If you make changes to the run conditions (such as SPEED and the run conditions.
  • Page 58 2-5-1 Programmed operation Step Key operation Screen display and considerations Use numeric keys to enter the memory • The image of the number where you have stored data. specified memory number appears. To call memory number 3, press ENTER >> If the contents of the memory number you have just called is not what you want, press ESC to switch back to the Program Map screen and then call another memory unit number.
  • Page 59 2-5-1 Programmed operation Step Key operation Screen display and considerations Start running the centrifuge under >> Run this machine according to 2-3-1 “Normal operation”. normal operation with no changes >> If you make changes to the run conditions (such as SPEED and made to the run conditions.

  • Page 60: Step-Mode Operation

    2-5-2 Step-mode operation 2-5-2 Step-mode operation This ultracentrifuge incorporates a step-mode operation feature, which stores two or more run conditions in one program memory area and switches between different values of speed, running time, temperature, and other parameters while in operation. This centrifuge can store up to nine steps. This section explains how to make settings by showing some examples.
  • Page 61 2-5-2 Step-mode operation Step Key operation Screen display and considerations While in the Run Screen, use the cursor keys to move the cursor to PROG RTC and press ENTER key. • Then move the cursor to PROG and press ENTER key. •...
  • Page 62 2-5-2 Step-mode operation Step Key operation Screen display and considerations • The message field in the Select “Creating or Changing.” bottom row switches to what you see on your ENTER left-hand side. • The display switches to Enter a desired condition. the Choose Rotor To select SPEED, press screen.
  • Page 63 2-5-2 Step-mode operation Step Key operation Screen display and considerations Lastly, specify run conditions for step • The entry of run conditions for step 3 is complete and step 4 is ready for into. (Downward cursor key) • The message field in the Press the downward cursor key >>...
  • Page 64 2-5-2 Step-mode operation Step Key operation Screen display and considerations Add Remark to the run conditions in >> When you press ESC key, the bottom row message is displayed. the memory. (1) A selection field to input or delete Remark appears. (1) Press the ESC key.
  • Page 65 2-5-2 Step-mode operation Step Key operation Screen display and considerations Press ESC key when the storing is over. Select “Running”. ENTER To run the centrifuge, select Yes. >> If you select “Yes”: ENTER • The display switches back to the Run Screen. •...
  • Page 66 2-5-2 Step-mode operation NOTE (1) Step display The PROGRAM field displays steps as follows: STEP : 1 --- 3 In the Run Screen, the run Final step conditions of this step are displayed (step 1 in this case). When the system finishes separating step 1 and moves to step 2, STEP : 2 --- 3 When the system moves from step 2 to the final step 3, STEP : 3 --- 3...
  • Page 67 2-5-2 Step-mode operation (2) Deleting a program This section explains how to delete a set of run conditions stored in program memory. To delete a memory number, delete all steps in that memory number. NOTE While the instrument is running, you cannot delete a program memory number. Step Key operation Screen display and considerations...
  • Page 68 2-5-2 Step-mode operation Step Key operation Screen display and considerations To delete this memory number, select >> If you select “Yes” Yes. • The system deletes all run conditions in ENTER memory number 3. (While in step mode, all ENTER steps get deleted.) >>...

  • Page 69: Rtc (Real-Time Control) Feature

    2-5-3 RTC (real-time control) feature 2-5-3 RTC (real-time control) feature The Discovery 100 SE and Discovery 90 SE ultracentrifuges contain an internal clock, allowing you to run the machine at a specified start or finish time for centrifugation. This feature for running the machine at a specified time is called the RTC (real-time control) feature.
  • Page 70 2-5-3 RTC (real-time control) feature 1. How to perform an RTC run Step Key operation Screen display and considerations • Blink the PROG • Set operational conditions while in the in the FUNCTION field. Run Screen. Use cursor keys to enter the SPEED part into an input wait, then press •...
  • Page 71 2-5-3 RTC (real-time control) feature Step Key operation Screen display and considerations Use cursor keys and numeric keys to enter month, day, hours, and minutes. Then, press ENTER key. >> To set the hours, use a number between 0 and 23 (in the 24-hour system).
  • Page 72 2-5-3 RTC (real-time control) feature NOTE 1. For an RTC run, the run mode indicator on the display panel displays the following: ACCEL DECEL START key ON Time STOP DELAY STOP Automatic start Fig. 2-5-6 Run mode display (RTC) 2. You cannot make an RTC setting in any of the following cases: (1) When the Run Screen is set to HOLD (continuous run) Set the run time (centrifugation time) not to HOLD but to a numerical value.
  • Page 73 2-5-3 RTC (real-time control) feature 2. Making changes to the RTC settings This section shows how to cancel or make changes to the RTC settings you have made. If you have already started an RTC run, press STOP to abort the RTC first. Press STOP to cancel the RTC run.
  • Page 74 2-5-3 RTC (real-time control) feature Step Key operation Screen display and considerations • The screen switches to a Making a change to date and time screen where you can settings enter a stop time (or start (1) Select “Setting stop time” or time).
  • Page 75 2-5-3 RTC (real-time control) feature Step Key operation Screen display and considerations Press cursor keys to blink RTC and then press ENTER key. ENTER • The message indicator in [Before starting the RTC run] the bottom row changes. (1) Select “Deleting the set RTC”. ENTER (2) Select “Yes”.
  • Page 76 2-5-3 RTC (real-time control) feature Step Key operation Screen display and considerations ii) Select "Cancel RTC-Start run". ENTER iii) Select "Continue RTC". • The display switches (3) Press ESC after process (2') i) or ii). back to the Run Screen. >>...

  • Page 77: Displaying And Setting Rcf

    2-5-4 Displaying and setting RCF 2-5-4 Displaying and setting RCF This ultracentrifuge stores the maximum and average radii of each rotor in internal memory. Setting a speed causes this centrifuge to automatically calculate and display the RCF (relative centrifugal force) value, while setting an RCF value causes the centrifuge to automatically calculate and display the speed.
  • Page 78 2-5-4 Displaying and setting RCF • When the rotor to be used is not identified (when Rotor type is displayed in the message display field) Step Key operation Screen display and considerations Set the speed on the Run Screen. >> See “2-2-1 Setting run conditions”. (1) Use cursor keys to move the cursor to RCF and press the...
  • Page 79 2-5-4 Displaying and setting RCF Step Key operation Screen display and considerations • The display switches to Select a desired rotor. the Used Rotor List To select a desired rotor from the screen. "Used Rotor List": • You can search for the rotor number you need in ENTER the order starting with the...
  • Page 80 2-5-4 Displaying and setting RCF Step Key operation Screen display and considerations (4) After checking the specifications of >> When you select “Yes” the rotor, select “Yes”. • The display switches back to the Run Screen ENTER and the selected rotor type is displayed in the message display field of the bottom row.
  • Page 81 2-5-4 Displaying and setting RCF 2. How to set an RCF value Enter a rotor number and an RCF value, and the machine will calculate, set, and display the speed. Shown below is the procedure. Step Key operation Screen display and considerations While in the Run Screen, set a speed.
  • Page 82 2-5-4 Displaying and setting RCF Step Key operation Screen display and considerations • The display switches to Select a desired rotor. the Used Rotor List To select a desired rotor from the screen. "Used Rotor List": • You can search for the (1) Enter No.
  • Page 83 2-5-4 Displaying and setting RCF Step Key operation Screen display and considerations (4') After checking the specifications of >> When you select “Yes” the rotor, select “Yes”. • The display switches back to the Run Screen ENTER and the selected rotor type is displayed in the message display field of the bottom row.
  • Page 84 2-5-4 Displaying and setting RCF Step Key operation Screen display and considerations • The value you have just Use cursor keys to move the cursor to entered appears in the the RCF setting field, and enter a RCF setting field. desired centrifugal force.

  • Page 85: Setting

    2-5-5 Setting 2-5-5 Setting This machine is provided with a function to perform an run. To perform an run, set the value instead of the run time. Time Step Key operation Screen display and considerations Set the Speed in the Run Screen. >>...
  • Page 86 2-5-5 Setting Step Key operation Screen display and considerations When you press START key, the • Arithmetic and integral machine starts to perform an operations are performed run. in the display field. display field NOTE When the display field reaches the set value of , the machine decelerates and stops.

  • Page 87: Defrost Function

    2-5-6 Defrost function 2-5-6 Defrost function If the chamber door is left open between low temperature runs, moisture from the ambient air humidity can condense (and even freeze) on the cold evaporator chamber walls. The potential for condensation increases as the ambient air temperature and humidity increases. If a run is performed with a significant amount of moisture in the evaporator chamber, the length of time required to reach a high vacuum will be increased.

  • Page 88: Setting And Resetting Zonal Operation

    2-5-7 Setting and resetting zonal operation 2-5-7 Setting and resetting zonal operation Selection between normal operation and zonal operation is performed by special operations using the FUNCTION field on the Run Screen. 1. Setting zonal operation Step Key operation Screen display and considerations On the Run Screen, use cursor keys to move the cursor to ZONAL in the FUNCTION field, and press...

  • Page 89: Rotor Management

    The warranted life of a Kendro Ultracentrifuge rotor is defined by a maximum number of runs. Other manufacturers use a combination of either the maximum number of runs or a maximum number of hours to limit the warranted life of the rotor.

  • Page 90: Automatic Rotor Deration

    2-6-2 Automatic rotor deration 2-6-3 Automatic rotor exclusion 2-6-2 Automatic rotor deration You can register a rotor in the ultracentrifuge before use. After the rotor has been registered, you can utilize the rotor management feature of the ultracentrifuge each time a run is made using the rotor. When the registered rotor comes close to the end of its life, the ultracentrifuge indicates it by displaying a message on the screen.

  • Page 91: Registering A Rotor

    2-6-4 Registering a rotor 2-6-4 Registering a rotor A standard rotor can be registered when you use the rotor management feature of the ultracentrifuge. If the rotor has not been registered, the feature does not operate for the rotor. To register a rotor, use the procedure described below.
  • Page 92 2-6-4 Registering a rotor Step Key operation Screen display and considerations Select “Creating or Changing”. • The display switches to the Rotor List screen. ENTER Select “Fixed Angle Rotor” in the Rotor • The display switches to List screen. the Fixed Angle Rotor List screen ENTER Select “T-890”...
  • Page 93 2-6-4 Registering a rotor Step Key operation Screen display and considerations Enter the total number of runs in the • The cursor moves to the Runs field. Hours field. You can (Do not press the ENTER key.) enter the number of hours.

  • Page 94: Deleting A Registered Rotor

    2-6-5 Deleting a registered rotor 2-6-5 Deleting a registered rotor If a registered rotor becomes unnecessary, you can delete it from ultracentrifuge memory. NOTE Once a registered rotor is deleted, all of the life management data for the rotor will be lost. As an example, suppose that you need to delete the T-890 fixed angle rotor registered in the previous section.

  • Page 95: Updating The Existing Data For A Rotor

    2-6-6 Updating the existing data for a rotor 2-6-6 Updating the existing data for a rotor When a rotor is registered in the Discovery 100 SE and the Discovery 90 SE ultracentrifuges without a rotor management function, both Total number of runs and Total number of hours can be updated. The updated value is accepted only when it is greater than the registered value.
  • Page 96 2-6-6 Updating the existing data for a rotor Step Key operation Screen display and considerations Enter the updated total number of runs and the total number of hours by using numeric keys. Total number of runs 500.0 505.0 Total number of hours 1200.4 1210.4 ENTER...

  • Page 97: When Power Failure Occurs

    2-7 When power failure occurs 2-7 When power failure occurs DANGER When removing the cover, table, etc. for maintenance, be sure to turn off the POWER switch of the centrifuge and wait for at least three minutes to avoid the possibility of an electrical shock.
  • Page 98 2-7 When power failure occurs (2) Turn off the POWER switch and power circuit breaker for the ultracentrifuge. (3) Remove the four M5 hexagon head bolts fixing the front cover from both sides and pull down the front cover by pulling its lower side forward. Then, remove the front cover. Top Deck Cabinet cover M5 hexagon head bolt...
  • Page 99 2-7 When power failure occurs Handle Vacuum release screw Power breaker Vacuum chamber Door unlock wire Fig. 2-7-2. Ultracentrifuge with the front cover removed M5 hexagon head bolt for attaching the front cover (4 pcs.) Fig. 2-7-3. Installing the front cover (7) Turn on the power breaker.

  • Page 100: Features Of The Menu Screen

    2-8 Features of the menu screen 2-8 Features of the menu screen Press MENU key on the key board, and the Menu Screen will appear. This feature is designed to allow you to use the Discovery 100 SE and Discovery 90SE with additional options including: (1) Centrifuge scheduler...
  • Page 101 2-8-1 Centrifuge scheduler Step Key operation Screen display and considerations Select “Reserve”. • The current date/time is displayed at the ENTER reservation time of the specified reservation No.3. Use cursor keys to move the cursor to the position at which you want to change the date/time of reservation, and enter a number by using numeric keys.
  • Page 102 2-8-1 Centrifuge scheduler Step Key operation Screen display and considerations Use cursor keys to move the cursor to • The ID Code input field is reservation No. you want to delete. displayed in the message (In this example, No. 3 is selected.) field in the bottom row.

  • Page 103: User List

    2-8-2 User List 2-8-2 User List The Centrifuge User List screen is displayed. This screen allows you to register, change, and delete any ID code and user name. • ID code and user name Password comprised of a 4-digit number. This number is not ID Code displayed when the centrifuge is operated.
  • Page 104 2-8-2 User List Step Key operation Screen display and considerations Select “Creating”. • The User List screen switches to the User ENTER Name screen. Use cursor keys to highlight a • Screen for entry of character and press the ENTER key to characters select it.

  • Page 105: Change Of User Name

    2-8-2 User List 2. Change of User Name NOTE Changing a User Name is limited to the person (with the same ID Code) who registered it and wants to change it. Step Key operation Screen display and considerations On the Menu Screen, select “User •...

  • Page 106: Deleting A User Name Setting

    2-8-2 User List Step Key operation Screen display and considerations After entering characters, use cursor • The cursor is positioned keys to move the cursor to SET and at SET . press the ENTER key to register the character string. •...

  • Page 107: Alarm Information

    2-8-3 Alarm information Step Key operation Screen display and considerations Select “Deleting”. • The ID code input field is displayed. ENTER Enter the ID Code used for registering • The confirmation No.9 by using numeric keys. message is displayed. Select “Yes”. •...

  • Page 108: Rotor Catalog

    2-8-4 Rotor Catalog 2-8-4 Rotor Catalog You can refer to rotor types and rotor specifications that are available. Step Key operation Screen display and considerations • The display switches to On the Menu Screen, select “Rotor the Rotor List screen. Catalog”.

  • Page 109: User Customizations

    2-8-5 User Customizations 2-8-5 User Customizations You can specify the language in which displays are given, set the current time, adjust screen contrast, and make other settings, all of which enable you to easily run the centrifuge. 1. Instrument identification number This function is intended to enter a centrifuge identification number (ID number).
  • Page 110 2-8-5 User Customizations (3) Changing date and time Use this option to precisely set the current time setting of the internal clock. Set an exact date and time for RTC operation. Use cursor keys and numeric keys and then press ENTER to make a setting.
  • Page 111 2-8-5 User Customizations (3) Start mode select This function allows you to select one of the following ACCEL DECEL values as the degree of vacuum in the process from 4,000 the vacuum wait to acceleration to the set speed: STOP WAIT STOP Medium vacuum: Approx.

  • Page 112: Print Utilities

    2-9 Print utilities (Optional) 2-9 Print utilities (Optional) This submenu option is used to print the various information maintained in the ultracentrifuge on the optional printer. The information includes run records, rotor usages, memory contents, etc. When you select “5. PRINT UTILITIES” from the “user customization routines” submenu, this submenu option starts working and displays the PRINT UTILITIES screen.
  • Page 113 2-9-1 Operating procedure Item Operating procedure Set run parameters (1) Select the corresponding item number on the Print Utilities Menu screen. Run result Set run parameters and result Print program (1) On the Print Utilities Menu screen, select “7. Print Program”. •...

  • Page 114: Contents Of Print Utilities

    2-9-2 Contents of print utilities 2-9-2 Contents of print utilities Item Example printout CENTRIFUGE RUN RECORDS >> Ultracentrifuge run records for a maximum of 40 runs can be printed out at a time. >> Once the ultracentrifuge run records are printed out, they are gone.
  • Page 115 2-9-2 Contents of print utilities Item Example printout SET RUN PARAMETERS PRINTOUT >> The currently set run parameters are printed out. RUN RESULT PRINTOUT >> The result of the last run made is printed out. Same as the examples of Nos. 4 and 5. RUN CONDITION/LOG OUTPUT >>...

  • Page 116: Automatic Run Result Printing

    2-9-3 Automatic run result printing Item Example printout USER LOG OUTPUT >> The centrifuge user's run log is printed out up to 20 runs. >> Once the user log is output, the previous run record is deleted and cannot be printed out once again. MACHINE MANAGEMENT OUTPUT >>...

  • Page 117: Printer Fault Handling

    2-9-4 Printer fault handling 2) Automatic printing of run records of rotor When the number of runs using a particular rotor with the ultracentrifuge has reached 20, the retained run records for the rotor will be automatically printed out. 3) Printout after each run When the PRINTOUT AFTER EACH RUN option is toggled on, the result of a run is automatically printed out upon completion of that run.

  • Page 118: Lockout (Optional)

    2-10 Lockout (Optional) 2-10 Lockout (Optional) NOTE When installing the Lockout Procedure for the first time, all memory is cleared. If obtaining Lockout Option after centrifuge has been installed, it will be necessary for service to record and then re-enter pertinent memory information such as total revolutions, total running count, and total running time.
  • Page 119 2-10 Lockout (Optional) 8. Press ENTER (Yes, register this User Name). 9. The number 1 is highlighted, press ENTER. 10. Input ID Code (4 digits), then press ENTER. 11. Input Password, then press ENTER. 12. Repeat step 6 thru 10 to input additional users. 13.

  • Page 120: Maintenance

    Service Decontamination Policy in this section. (3) If the instrument, rotor and/or accessories require servicing by Kendro either at the customer's site or at Kendro's facilities, disinfect and decontaminate it following the Service Decontamination Policy in this section.

  • Page 121: Rotor Chamber

    3-1 Rotor chamber 3-2 Drive spindle 3-3 Cabinet 3-1 Rotor chamber To maintain the rotor chamber, follow the instructions given below: (1) When the ultracentrifuge is not in use, keep the rotor chamber ventilated. (2) If the bowl is moist, wipe it with a clean, dry cloth or sponge. (3) If the rotor chamber is dirty, wipe it with a clean cloth or sponge dampened with a diluted solution of mild, non-alkaline detergent.

  • Page 122: Replacement Parts

    1. Clean the centrifuge to be serviced of all encrusted material and decontaminate (see Maintenance Section of centrifuge) it prior to servicing by the Kendro representative or returning it to the Kendro facility. There must be no radioactivity detectable by survey equipment.
  • Page 123 If the centrifuge to be serviced does not have a Decontamination Information Certificate attached and, in Kendro's opinion presents a potential radioactive or biological hazard, the Kendro representative will not service the equipment until proper decontamination and certification is complete. If Kendro receives a centrifuge at its Service facilities which, in its opinion, is a radioactive or biological hazard, the sender will be contacted for instructions as to disposition of the equipment.

  • Page 124: Troubleshooting

    Make sure to notify the service representative of the use of such materials then. CAUTION Do not operate or run the instrument in any way other than specified in this manual. If you encounter any problem with the instrument, call an authorized Kendro Field Service Representative. 4 --- 1...

  • Page 125: Alarm Indicators

    4-1 Alarm indicators This ultracentrifuge incorporates a “self-diagnosis feature” that diagnoses the cause of any problem which may occur when you start the centrifuge or while in operation. 4-1 Alarm indicators If any trouble occurs, this machine gives a buzzer sound and display an alarm message in the message field of the Run Screen to warn of the trouble.
  • Page 126 If an alarm message appears, remove the cause of the problem as described below and press CE key. You will then be able to resume your centrifugation. If the alarm message persists even after you have done what is specified below, contact a Kendro service representative.

  • Page 127: Diagnosed Problems - Requiring Maintenance

    4-3 Non-diagnosed problems 4-2 Diagnosed problems - requiring maintenance When a problem except those listed in Section 4-1 is reported on the message display at the bottom of the screen, call your authorized Kendro representative and communicate the displayed problem message. NOTE When the "No.

  • Page 128: Preinstallation

    The information on these two pages describes the location requirements and the electrical services that you must make provision for prior to installation of the centrifuge by an authorized Kendro Field Service Engineer. The requirements may be modified, if necessary, to comply with state or local ordinances.

  • Page 129: Electrical Requirements

    5. Installation 5-2 Electrical Requirements The centrifuge must be connected to the power source that is specified on the nameplate on the left side of the instrument panel. The centrifuge can be wired for split two-phase or split three-phase application and international color coding of wires used, as appropriate.

  • Page 130: Moving The Ultracentrifuge

    5. Installation Frame Caster Leveling screw Fig. 5-2 Leveling Adjustment (3) Place the level (part no. 45216) across the top of the drive shaft in the rotor chamber (see Fig. 5- 3). Turn the four leveling screws until the bubble in the level indicates the instrument is level. (4) When the instrument is level, check that the four leveling screws are secured to the floor.

  • Page 132: Specifications

    6. Specifications 6. Specifications Model Discovery 100 SE Discovery 90 SE Maximum speed 100,000 rpm 90,000 rpm Maximum centrifugal 802,006 x g 692,149 x g force Speed control 10 rpm (1,000 rpm to maximum speed) accuracy Time for acceleration About 5 minutes from 0 to 100,000 rpm or vice versa and deceleration Rotor temperature 0.5 ˚C (set temperature is from 0 ˚C to 40 ˚C)
  • Page 133 6. Specifications The Discovery 100 SE and Discovery 90 SE ultracentrifuges satisfy CE marking requirements. The CE marking is an international symbol which shows that the product conforms to EC directives. 6 --- 2...

  • Page 134: Supply List

    7. Supply list 7. Supply list The items listed below are those supplied with the ultracentrifuge. Item name Part no. Q'ty Item drawing Remarks Instruction manual 45912 Vacuum grease 65937 Supplied in 1-liter Vacuum pump oil 45128 NEO VAC containers (MR100) M5 screw Hex.

  • Page 136: Warranty

    8. Warranty 8. Warranty KENDRO LABORATORY PRODUCTS, L.P. MAKES NO WARRANTY OF ANY KIND, EXPRESSED OR IMPLIED, INCLUDING THAT OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE EXCEPT AS STATED IN THIS WARRANTY POLICY STATEMENT. Subject to the exceptions and upon the conditions specified in this...
  • Page 137 Kendro, which are inconsistent or in conflict with the terms of this warranty (including but not limited to the limitations of the liability of Kendro as set forth above), shall not be binding upon Kendro unless reduced to writing and approved by Kendro.
  • Page 138 1 (203) 270-2115 Tel.: 1 (203) 270-2080 1 (203) 270-2110 Fax: 1 (203) 270-2210 e-mail: info@kendro.com 1 (203) 270-2166 e-mail: info@kendro.com Or contact a local representative for SORVALL ® brand products. Visit our web site at http://www.kendro.com or http://www.kendro.de Rev. 01/03...

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