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How to use this manual Use this manual to get acquainted with your centri- fuge and its accessories. This manual helps you to avoid inappropriate han- Overleaf you will find a graphic dling. Make sure to keep it close to the centrifuge. representation of the control panel with a survey of the most important A manual that is not kept handy cannot provide...
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(only for instruments with refrigeration unit) acceleration deceleration temperature profiles profiles run time Quick run speed/RCF program selection open lid stop start rpm/RCF pre-temperature-regulation Bucket selection switch key "set" keys "set" keys display Before switching on the centrifuge please read this manual The mains switch is located on the right-hand side panel.
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® Control panel of the Multifuge 1 S / 1 S-R Keys start : normal start of the centrifuge Display panels stop : manual stop of a run Program selection open lid: open lid key 1 - 4 : store or recall programs. (possible only with the instrument switched on) acceleration profiles (1= slowest ..
Contents Contents For your safety..........3 Operation ..........37 Proper use..............3 Switching on the centrifuge ........37 Improper use ............3 Actuating the lid............37 Centrifuging hazardous materials ......3 Opening the lid ........... 37 Handling the centrifuge ..........4 Closing the lid .............
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Audible alarm ............52 Turning off the centrifuge ........52 Maintenance and care ......53 Maintenance to be performed by the customer ..53 Cleaning.............. 53 Disinfection ............54 Decontamination..........56 Autoclaving ............56 The KENDRO service offer ........57 Warranty conditions..........57...
For your safety For your safety Proper use Heraeus centrifuges are manufactured according to The centrifuge is designed to separate liquid- current technical standards and regulations. Nonethe- suspended materials having different densities and less, centrifuges may pose danger to individuals and particle size, respectively (maximum sample density is surrounding if 1.2 g/cm³...
• Changes in mechanical or electrical components of the centrifuge may only be carried out by individuals Handling the centrifuge authorized by Kendro Laboratory Products. • Use only original accessories for the centrifuge. The only exceptions are common glass or plastic centri- fuge tubes if they are approved for the rotor speed and RCF values.
For your safety Conformity to current standards Safety instructions in this manual Heraeus centrifuges are manufactured and tested This symbol denotes potential hazards to according to the following standards and regulations: persons. - For all voltages This symbol denotes potential damage to •...
® Description of the Multifuge ® The Multifuge 1 S / 1 S-R Description ® ® The figure below shows a Multifuge 1 S-R with an The Multifuge 1 S-R is a general-purpose tabletop centrifuge for biotechnological and pharmaceutical opened lid and a swinging bucket rotor installed. research that moves high capacity centrifugation onto the fast track.
® Description of the Multifuge Safety systems Parts supplied ® The Multifuge is equipped with a number of safety Accessories supplied with the centrifuge are: systems: − power cord • Housing and lid are constructed of 6 mm steel. • Lid with window −...
® Description of the Multifuge Function and features Basic unit/ function Description / feature Cabinet / frame Galvanized steel Chamber Stainless steel * (Multifuge 1S: coated steel) Drive Brushless induction drive Key pad and display Key pad and display elements covered by an easy-care continuous surface Control Microprocessor driven by "EASYset"...
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® Description of the Multifuge The EASYset user interface Function Feature Program memory keys Freely programmable Acceleration / deceleration profile 1 = slowest, ... 9 = fastest acceleration / deceleration curve Setting speed by rpm Adjustable from 300 rpm to 15 000 rpm, in 10 rpm increments RCF selection Upon actuation of RCF switch , the RCF value can then be entered Time selection...
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® Description of the Multifuge Function Feature Lid opening Automatic unlocking via ”open lid" key ( (unlocking in case of power failure: see chapter ”Troubleshooting") Start Start key ( Stop Stop key ( "Quick Run" mode Pressing the "quick run" key ( ) activates maximum acceleration up to the maximum permissible speed of rotor;...
Before use Before use The centrifuge can be damaged by Centrifuge transport and installation jolting during the transport! After opening the box remove the protective materials. Transport the centrifuge only in the upright position using proper con- tainment and secure it properly. When transporting centrifuge,...
Before use Main connection • The centrifuge must be protected from heat and direct sunlight. Connect the centrifuge only to a grounded main power • The location must be well ventilated at all times. supply. Make sure that the power cord is compatible with the valid safety regulations and that your main voltage and frequency correspond to the specifications UV rays reduce the durability of...
Use caution when touching rotors as For more information you can visit our web site at they may be hot after long runs at http://www.Kendro.com high speed.
Rotors and accessories ® Rotors for the Multifuge ® Table 1: Rotors for Differences of 120V instruments are shown in parentheses Multifuge Rotor designation Swinging Bucket Rotor TTH 400 75002000 With bucket Rectangular bucket Round bucket 150 ml 400 ml Order no.
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Rotors and accessories ® Table 1: Rotors for Differences of 120V instruments are shown in parentheses Multifuge Rotor designation Swinging Bucket Rotor TTH 400 75002000 With bucket Multiple carriers Multiple carriers 14 x 15 ml 5 x 50 ml + 2 x 15 ml Order no.
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Rotors and accessories ® Table 1: Rotors for Multifuge Differences of 120V instruments are shown in parentheses ® Rotor designation BIOshield 600 Rotor Micro plate carrier 4 x 150 ml MP 3300 Order no. 75002005 75002010 Maximum permissible load [ g ] 4 x 540 2 x 600 Maximum speed n...
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Rotors and accessories ® Table 1: Rotors for Multifuge Differences of 120V instruments are shown in parentheses ® Fixed-Angle Rotor FA12.94 Highconic Micro Liter Rotor Rotor designation 6 x 94 ml / 12 x 16 ml 48 x 2 ml 75002006 75003348 Order no.
Rotors and accessories ® Rotors for the Multifuge 1 S-R ® Table 2: Rotors for Differences of 120V instruments are shown in parentheses Multifuge 1 S-R Rotor designation Swinging Bucket Rotor TTH 400 75002000 With bucket Rectangular bucket Round bucket 150 ml 400 ml Order no.
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Rotors and accessories ® Table 2: Rotors for Differences of 120V instruments are shown in parentheses Multifuge 1 S-R Rotor designation Swinging Bucket Rotor TTH 400 75002000 With bucket Multiple carriers Multiple carriers 14 x 15 ml 5 x 50 ml + 2 x 15 ml Order no.
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Rotors and accessories ® Table 2: Rotors for Multifuge 1 S-R Differences of 120V instruments are shown in parentheses ® Rotor designation BIOshield 600 Rotor Micro plate carrier 4 x 150 ml MP 3300 Order no. 75002005 75002010 Maximum permissible load [ g ] 4 x 540 2 x 600 Maximum speed n...
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Rotors and accessories ® Table 2: Rotors for Multifuge 1 S-R Differences of 120V instruments are shown in parentheses ® Fixed-Angle Rotor FA12.94 Highconic Micro Liter Rotor Rotor designation 6 x 94 ml / 12 x 16 ml 48 x 2.0 ml 75002006 75003348 Order no.
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Rotors and accessories Adapter Table 3: Adapter (1) * Max. tube length with aerosol-tight cap Max. tube Tubes Color Order no. Adapter and accessories for dimensions diameter rectangular buckets 7500 2001 x length/ * [ mm ] rotor [mm] ® Centri-Lab Adapter type A plus 14 x 7 ml blood sampling...
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Rotors and accessories Table 3: Adapter (2) * Max. tube length with aerosol-tight cap Max. tube Adapter and accessories for Tubes Color No. of order dimensions diameter x length / * round buckets 7500 2002 [mm] rotor [mm] ® Centri-Lab Adapter type E 34 x 1.5 / 2 ml micro liter tube 10.5 x 46...
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Rotors and accessories Table 3: Adapter (3) * max. tube length with aerosol-tight cap Max. tube Adapter and accessories for Tubes Color No. of order dimensions diameter ® x length / * BIOshield 600 Rotor 7500 2005 [ mm ] rotor [mm] ®...
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Rotors and accessories Table 3: Adapter (4) Max. tube Number per Number Color No. of Adapter and accessories for dimensions adapter order Fixed-Angle Rotor FA12.94 x length rotor 7500 2006 [mm] Adapter for 94 ml cavity 1.5 ml micro tube 11 x 58 nature 7600 2905...
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Rotors and accessories Tabelle 3: Adapter (5) Accessories for No. of Micro plate carrier MP 3300 order 7500 2010 Aerosol-tight cap 2 pieces, incl. seals and lubricants 7500 2011 Adapter for micro plate carrier 7500 2013 Spare seal 2 pieces, incl. lubricants 7500 2012 Table 3: Adapter (6) Max.
Rotors and accessories Handling the rotor Swinging Bucket Rotor TTH 400 ® BIOshield 600 Rotor Micro plate carrier All positions must always be loaded with identical carrier buckets! The various swinging buckets are split up into weight categories. These can be identified through the letters suffixing the order number on the bucket.
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Rotors and accessories ® BIOshield Rotor 7500 2005 Do not run the rotor without the rotor Always maintain the rotor in the recom- cover installed. mended manner. The rotor and accessories must be cleaned and inspected regularly: do not use when ®...
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Rotors and accessories Micro plate carrier MP 3300 7500 2010 Remove the appropriate plate holder from the carrier body for loading and unloading of The operation is permissible only micro plates in the Micro plate carrier. with the associated carriers! (Label "75002010") Some commercially available micro- plates are not rated for the maximum...
Rotors and accessories Aerosol-tight operation Use the special lubricant 7600 3500 only to grease the seals! Aerosol-tight rotors and tubes are only to be opened in an approved safety work bench when centrifuging danger- Spare parts are delivered with the rotor or may be or- ous samples! dered separately.
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Rotors and accessories Closing the rectangular bucket 75002001 Closing the Micro test plates rotor MP3300 aerosol-tightly 75002010 aerosol-tightly After putting on the cap both levers are pressed down until they noticeably click in. Please flap both lock levers upward. The cap can now be easily put on the bucket.
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Rotors and accessories Closing the round bucket 75002002 Closing Fixed-Angle Rotor aerosol-tightly aerosol-tightly The hexagon wrench should be used as a support tool to fasten and loosen the lid of the fixed angle rotor in After greasing the seal, turn the lid until it sits lightly on order to achieve secure closing (insert the hexagon the bucket.
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Rotors and accessories Rotor Vessel type / maximum filling volume Micro 1,5 ml Micro 2,0 ml Micro Liter Rotor 48 x 2.0 ml 75003348 1,0 ml 1,5 ml Fixed-Angle Rotor FA12.94 Falcon 50 ml Falcon 15 ml 49 ml 14 ml 75002006 others: - 2/3 nominal volume...
Rotors and accessories • Shaking the bucket releases the carbon dioxide of Checking of aerosol-tight bio-containment the water, and an excessive pressure is built up. The checking of the rotor type and bucket was done • Leaks are recognized by humidity release and according to the dynamic microbiological test proce- audible disinflation of gas mix.
Operation Operation Switching on the centrifuge Locate the main power switch on the right-hand side of the front panel, and set it to the ON (I) position. For a Actuating the lid couple of seconds the following reading appears in the control panel: Opening the lid Press the "open lid"...
Operation 4. The rotor must glide freely down the collet chuck Installing the rotor until it hits the lower stop. 5. If you have positioned the rotor correctly, you can Improper or improperly combined tighten the collet chuck easily using the supplied accessories may cause severe dam- hex wrench.
Operation If you wish to centrifuge samples that, together with the Loading the rotor adapters, exceed the maximum permissible load, you Maximum loading must either reduce the sample volume or calculate the permissible speed n according to the following for- perm Overloading can result in destruction mula:...
Operation Filling the centrifuge tubes Maximum permissible load difference The smaller the imbalance of the centrifuge, the better the separation effect, because as Check carefully whether your tubes imbalance is minimized, so is the resulting are approved for the respective RCF value.
Operation Inserting the centrifuge tubes Fixed-angle rotors: The rotor must be loaded symmetri- cally. Failure to do so can cause rotor imbalance, which may lead to noisy operation, affect separation quality, or result in imbalance detection shut- down, as well as introduce significant detrimental wear to the motor and drive system.
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Operation Swinging bucket rotors: Improper loading These examples are to be applied to the other rotors in an analogous manner! Proper loading...
Operation Bucket selection for swinging bucket Entering parameters rotors Deceleration curves When running a swinging bucket rotor, the automatic ® The Multifuge offers 9 acceleration and deceleration rotor identification feature will recognize the rotor body. profiles for optimal centrifuging of samples and gradi- Depending on the bucket or carrier installed on the ents.
Operation number of seconds, then changes to a permanent Selecting speed display. The speed is now stored. The centrifuge speed can be set to a minimum of 300 rpm and to a maximum of 15 000 rpm (depending on For faster operation, you may shift the flash- the rotor).
Operation More about the RCF value Selecting run time The relative centrifugal force (RCF) is given in multi- There are two time modes: standard and extended. ples of the earth gravity g. It is a dimensionless number In the standard time mode you can select a run time that allows comparing the efficiency of separation or between 1 min and 9 h 59 min or continuous operation sedimentation of diverse instruments since it is inde-...
Operation If you keep the selected key pressed, the dis- Extended time mode play changes slowly at first and at an acceler- You have the option of switching to the extended time ated pace after a few seconds. mode. To switch it on or off press the program selec- Release the key as soon as you have reached tion key the desired value, and adjust if necessary by...
Operation Pretemp function Selecting the temperature The Pretemp function permits easy and quick pre- You can select the temperature in the range of -9 °C to temperature-regulation of the empty rotor. +40 °C. Upon calling this function by pressing the key , all To adjust the temperature, proceed as follows: you have to do is enter the desired temperature.
Operation Starting the centrifuge Imbalance display Once the rotor is properly installed, the main switch is If rotor imbalance is detected, shortly after the rotor turned on and the lid is closed, you can start the centri- reaches 300 rpm, the message "bAL" will appear in the fuge.
Operation Stopping the centrifuge Temperature control during standby Stopping with preset run time Temperature control becomes active once the rotor has been identified. This is the case after a centrifuga- Normally the run time has been selected, and all you tion run exceeding 300 rpm.
Operation Centrifuging with a program Working with programs After closing the centrifuge lid, call the desired program The 4 program selection keys offer the option of storing memory number using the program selection key and and recalling the individual centrifugation processes. press the start key If the rotor is started with a program the speed or RCF set point value which exceeds the permissible value of...
Operation ”Quick Run” Removing the rotor ® For short-time operation the Multifuge is equipped 1. Open the centrifuge lid. with a "quick run" function 2. Remove the rotor cover (on applicable rotors). Short-time centrifugation is started by pressing the 3. Unscrew the clamping sleeve counterclockwise "quick run"...
Operation Audible alarm Turning off the centrifuge Accompanying all error messages, a warning signal is The centrifuge is turned off by switching the main given out which can be silenced upon pressing any switch into the "0"position. key. The main power switch should be turned off You have the option of signaling the end of a run after a complete centrifugation run.
Clean them with mild agents of pH values ranging from 6 to 8. If you intend to use cleaning agents or disinfection procedures not rec- For other cleaning agents please consult KENDRO ommended by the manufacturer, you Services! have to ensure that the foreseen pro-...
Maintenance and care Disinfection If a centrifuge tube containing infectious material leaks During cleaning, liquids and espe- during a run, you have to disinfect the centrifuge im- cially organic solvents should not mediately. come into contact with the drive shaft and the ball bearing. Organic solvents may decompose Infectious material could enter the the lubricant of the motor bearing.
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7. Dispose of the disinfectant according to valid regu- lations. 8. Aluminum rotors have to be treated with anticorro- For other disinfectants please consult KENDRO sive protective oil subsequently. Services! • You may disinfect the rotor and the accessories as Disinfection with bleaching lye described in the following section.
Maintenance and care Decontamination For general radioactive decontamination, use a solu- Chemical additives to the steam are not tion of equal parts of 70% ethanol, 10% SDS and wa- permitted. ter. Follow this with ethanol rinses, then de-ionized water rinses, and dry with a soft absorbent cloth. Dis- pose of all washing solutions in appropriate radioactive Never exceed the maximum permis- waste containers!
• the rotor fastening and the drive shaft thorized by KENDRO Defective material is exchanged. • the required maintenance and care procedures are KENDRO offers inspection and service contracts cov- carried out regularly. ering it. Inspection costs are charged as flat-rate con- tracts.
Troubleshooting Troubleshooting Proceed as follows: 1. Make sure that the rotor is at a complete stop Emergency lid release (observe through window in the cover). In case of a power failure the lid cannot be opened normally using the electrical lid unlocking mechanism During a power outage, it is impossible To permit unloading in this case, the centrifuge is to lock the lid once the emergency lid...
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Troubleshooting 4. Afterwards, push the cord back into the instrument and reinsert the plastic plug. Once the power is restored, you can connect the in- strument to the main supply and turn it on. Following the self test of the centrifuge, the lid may be closed and locked with the motor.
1. Is the mains plug turned on? deceleration. The lid 2. Check the main connection. cannot be opened. 3. If the main connection is ok, contact the nearest KENDRO service station. Displays fail briefly. The drive stops suddenly. Main connection was briefly interrupted The rotor stops without 1.
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3. Check whether a broken tube or damage to the rotor was responsible for imbalance switch actuation. 4. Check that the trunnions of the swinging bucket rotor have been properly lubricated. If you cannot locate and solve the problem yourself, contact the KENDRO service station.
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Troubleshooting Error Symptom Possible causes and corrective measures Message "rotor" appears Rotor decelerates with Set speed exceeds permissible maximum speed for the in display. delayed deceleration. rotor. (The same holds for RCF setting) A) For about 15 sec. the display shows alternately "rotor" and the maximum permissible speed or RCF for the installed, after the rotor identification.
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Rotor stops with Temperature measurement error. deceleration to standstill. Switch the instrument off and on again. If the problem Instrument cannot be persists, contact a KENDRO service station. E-16 operated. E-17 Rotor stops with Maximum speed for the rotor identification exceeded.
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Troubleshooting Error Symptom Possible causes and corrective measures E-19 Instrument stops with No rotor present or rotor identification impossible. deceleration to standstill A) Check if a certified rotor is inserted. after short starting-up. B) Please pay attention to the legibility of the inscription of the swinging bucket rotor cross when it is installed.
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Rotor stops without Error in speed measurement deceleration to standstill. Switch the instrument off and on again. If the error persists, Instrument cannot be contact the KENDRO service station E-23 operated. E-24 Instrument cannot be Wrong status information from lid lock.
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Motor or rotor blocked. 1. Switch instrument off and on again using the main switch. 2. Open the lid. 3. Check whether the rotor can turn freely. If you cannot clear the malfunction, contact the KENDRO service station. E-30 Rotor stops without Control voltage breaks down.
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Check and clean ventilation slots if necessary. After about 60 min. you can restart the instrument. Observe the max. permissible environmental temperature! If the error persists, contact the KENDRO service station. E-34 Rotor stops without Overvoltage in the intermediate circuit.
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Instrument cannot be Significant imbalance caused by missing bucket operated. Tubes or adapter do not fit (rotor is rubbing the chamber or the cover) Micro plates carriers on the motor cover If the error persists, contact the KENDRO service station.
Troubleshooting Subsequently, the following readings will be dis- Contacting Kendro Service played for 5 seconds each: Should you require help from our service station, (Numbers are examples) please indicate the catalog and serial number of your instrument. You will find the pertinent information at the Software version keyboard specifications, near the socket for the main plug.
Technical Data Technical Data Features Specification - indoor use Ambient conditions - maximum elevation 2000 m (6562 ft) above sea level - max. relative humidity 80 % up to 31°C (88°F), linearly decreasing down to 50 % relative humidity at 40°C (104°F). Ambient temperature allowed +2 °C to +40 °C (36°F to 104°F)
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Technical Data Features Specification ® Weight without rotor Multifuge 92 kg ( 203 lb ) ® Multifuge 1 S-R 118 kg ( 259 lb ) Testing standards - all devices manufactured and examined in agreement with: IEC 61010-1:1990 + amendment 1:1992 + amendment 2:1995 IEC 61010-2-020:1993 + amendment 1:1996 - Pollution degree 2, - Overvoltage category II - for 120 V only...
Technical Data Electrical connections / fuses Frequency Nominal Power Fuse protection of Fuse Order no. Voltage current consumption instrument protection of building safety fuse * thermal excess current release ® Multifuge 12 A 230 V 50/60 Hz 9.1 A 1300 W 16 AT 7500 4311 * 14 A...
Appendix Acceleration and deceleration profiles On the following pages you find acceleration and deceleration profiles for each rotor type respectively.
Index acceleration and deceleration curves · 75 centrifuge exceptionally noisy · 62 acceleration profiles · 7 Centrifuge transport and installation · 13 Acceleration/decelaration profile · 10 Centrifuging with a program · 50 actual values · 44 changing settings adapter · 24 during run ·...
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icons maintenance · 53 for denoting dangers and potential damage · 5 manual lid unlocking · 59 imbalance · 40, 41 manual stopping · 49 imbalance · 48 max. tube dimensions · 25 imbalance detection · 8 maximum filling volume · 35 indoor use ·...
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RCF / speed display · 43 RCF display and rotor identification · 44 pathogenic microorganisms RCF selection · 10 protection against · 4 RCF setpoint · 44 Plastic sample tubes RCF value · 7, 45 Pretemp function · 47 relative centrifugal force · 45 pretemperature-regulations ·...
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Safety instructions · 5 speed of centrifugation safety measures · 3 for calculation of RCF value · 45 safety standards · 5 Speed selection, · 10 safety zone · 3, 13 speed setting · 44 sample density Starting the centrifuge · 48 maximum ·...
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time mode · 46 maximum filling · 35 tube breakage with infectious material · 54 Turning the centrifuge off · 52 warning signal · 52 warranty conditions · 57 wear · 4 volume...
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Kendro Laboratory Products International Sales · Asheville, NC · USA · Tel. +1 828-658 2711 · Fax +1 828-645 7581 · info@kendro.spx.com North America Kendro Laboratory Products International Sales · Asheville, NC · USA · Tel. +1 828-658 2711 · Fax +1 828-645 3368 · info@kendro.spx.com Sorvall and Heraeus products ·...
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