Page 2
How to use this manual Use this manual to get acquainted with your centri- fuge and its accessories. The manual helps you to avoid inappropriate han- Overleaf you will find a graphic dling. Make sure to keep it always close to the centri- representation of the control panel fuge.
Page 3
(only for instruments with refrigeration unit) deceleration temperature run time Quick run profiles speed/RCF open lid stop start rpm/RCF Bucket selection switch key Set value input display Before switching on the centrifuge please read this manual The mains switch is located on the right-hand side panel.
Page 4
Control panel of the Legend™ T / RT Keys start : normal start of the centrifuge Display panels stop : manual stop of a run Deceleration profiles (1= slowest ..3= fastest) open lid: open lid (possible only with the instrument permanent switched on) display:...
Contents Contents For your safety..........3 Operation ..........41 Proper use..............3 Switching on the centrifuge ........41 Improper use ............3 Actuating the lid............41 Centrifuging hazardous materials ......3 Opening the lid ........... 41 Handling the centrifuge ..........4 Closing the lid .............
Page 6
Deceleration profiles..........79 Maintenance and care ......57 Speed/RCF diagrams..........85 Maintenance to be performed by the customer ..57 Index ............91 Cleaning.............. 57 Disinfection ............58 Decontamination..........60 Autoclaving ............60 The KENDRO service offer ........61 Warranty conditions..........61...
For your safety For your safety Proper use ® The SORVALL centrifuges are manufactured accord- The centrifuge is designed to separate liquid- ing to current technical standards and regulations. suspended materials having different densities and Nonetheless, centrifuges may pose danger to individu- particle size, respectively (maximum sample density is als and surrounding if 1.2 g/cm³...
• Changes in mechanical or electrical components of protective tubes. the centrifuge may be carried out only by individuals authorized by Kendro Laboratory Products. Handling the centrifuge • Use only original accessories for the centrifuge. The only exception are common glass or plastic centri- fuge tubes, if they are approved for the rotor speed and RCF values.
For your safety Conformity to current standards Safety instructions in this manual ® SORVALL centrifuges are manufactured and tested This symbol denotes potential hazards to according to the following standards and regulations: persons. - for all voltages This symbol denotes potential damage to •...
Description of the Legend™ The Legend™ T / RT Description The figure below shows a Legend™ with the lid The Legend™ is a general-purpose tabletop centri- open and a swinging bucket rotor installed. fuge for biotechnological and pharmaceutic research that moves high capacity centrifugation onto the fast track.
Description of the Safety systems Parts supplied The Legend™ is equipped with a number of safety Accessories supplied with the centrifuge are: systems: − power cord • Housing and lid is constructed of 8 mm steel. • Lid with window −...
Description of the Legend™ Function and features Basic unit/ function Description / feature Cabinet / frame galvanized steel Chamber stainless steel Drive Brushless induction drive Key pad and display key pad and display elements covered by an easy-care continuous surface ®...
Page 14
Description of the ® The QUIKset user interface Function Feature Deceleration profile 1 = slowest, ... 3 = fastest deceleration curve Setting speed by rpm adjustable from 300 rpm to 15 000 rpm, in 10 rpm increments RCF selection upon activation of RCF switch , the RCF value can then be entered Time selection adjustable in minutes from 1 min to 9 h 59 min, or extended time mode from 1 min to 99 h,...
Page 15
Description of the Legend™ Function Feature Lid opening automatic unlocking via ”Open lid“ key ( (unlocking in case of power failure: see chapter ”Troubleshooting“) Start start key ( Stop stop key ( "Quick Run“ mode pressing the "Quick Run“ key ( ) activates maximum acceleration up to the maximum permissible speed of rotor;...
Before use Before use The centrifuge can be damaged by Centrifuge transport and installation jolting during the transport! After opening the box remove the protective materials. Transport the centrifuge only in the upright position using proper con- tainment and secure it properly. When transporting centrifuge,...
Before use Main connection • The centrifuge must be protected from heat and direct sunlight. Connect the centrifuge only to an grounded main • The location must be well ventilated at all times. power supply. Make sure that the power cord is com- patible with the safety regulations valid, and that your main voltage and frequency correspond to the specifi- UV rays reduce the durability of...
For more information you can visit our web site at http://www.Kendro.com Legend™ T (air cooled) During centrifugation, heat is generated by air friction between the rapidly spinning rotor and the air inside the rotor chamber.
Rotors and accessories Rotors for the Legend™ T Table 1: Rotors for Legend™ T 100V / 120V (differences of 230V instruments are shown in parentheses) Rotor designation Swinging Bucket Rotor 7500 6445 (7500 6434 with integrated locking mechanism) With bucket Round bucket Rectangular bucket Carrier for...
Page 21
Rotors and accessories Table 1: Rotors for Legend™ T (differences of 230V instruments are shown in parentheses) 100V / 120V Rotor designation Swinging Bucket Rotor 7500 6445 (7500 6434 with integrated locking mechanism) Tissue culture bucket Multiple carriers Multiple carriers With bucket 250 ml 25 x 5 / 7 ml...
Page 22
Rotors and accessories Table 1: Rotors for Legend™ T (differences of 230V instruments are shown in parentheses) 100V / 120V Rotor designation Swinging Bucket Rotor 7500 6445 (7500 6434 with integrated locking mechanism) With bucket Multiple carriers Multiple carriers 17 x 15 ml 7 x 50 ml Order no.
Page 23
Rotors and accessories Table 1: Rotors for Legend™ T 100V / 120V (differences of 230V instruments are shown in parentheses) ® ® Rotor designation BIOshield Rotor Highplate Rotor 4 x 250 ml 2 x 5 plates Order no. 7500 6435 7500 6444 Maximum permissible load [ g ] 4 x 600...
Page 24
Rotors and accessories Table 1: Rotors for Legend™ T 100V / 120V (differences of 230V instruments are shown in parentheses) ® Fixed-Angle Rotor Highconic Rotor Rotor designation 6 x 94 ml 6 x 50 ml 7500 3334 7500 3057 Order no. Maximum permissible load [ g ] 6 x 140 6 x 130...
Rotors and accessories Rotors for the Legend™ RT Table 2: Rotors for Legend™ RT (differences of 200V / 230V instruments are shown in parentheses) 120V Rotor designation Swinging Bucket Rotor 7500 6445 (7500 6434 with integrated locking mechanism) With bucket Round buckets Rectangular buckets Carrier for...
Page 26
Rotors and accessories Table 2: Rotors for Legend™ RT (differences of 200V / 230V instruments are shown in parentheses) 120V Rotor designation Swinging Buckets Rotor 7500 6445 (7500 6434 with integrated locking mechanism) With bucket Tissue culture bucket Multiple carriers Multiple carriers 250 ml 25 x 5 / 7 ml...
Page 27
Rotors and accessories Table 2: Rotors for Legend™ RT (differences of 200V / 230V instruments are shown in parentheses) 120V Rotor designation Swinging Buckets Rotor 7500 6445 (7500 6434 with integrated locking mechanism) With bucket Multiple carriers Multiple carriers 17 x 15 ml 7 x 50 ml Order no.
Page 28
Rotors and accessories Table 2: Rotors for Legend™ RT (differences of 200V / 230V instruments are shown in parentheses) 120V ® ® Rotor designation BIOshield Rotor Highplate Rotor 4 x 250 ml 2 x 5 plates Order no. 7500 6435 7500 6444 Maximum permissible load [ g ] 4 x 600...
Page 29
Rotors and accessories Table 2: Rotors for Legend™ RT (differences of 200V / 230V instruments are shown in parentheses) 120V ® Micro Liter Rotor Fixed-Angle Rotor Highconic Rotor Rotor designation 24 x 2.0 ml 6 x 94 ml 6 x 50 ml 7500 3332 7500 3334 7500 3057...
Page 30
Rotors and accessories Adapter Table 3: Adapter (1) * max. tube length with aerosol-tight cap max. tube Adapter and accessories for tubes color order no. dimensions diameter d x length / * round buckets 7500 6441 [ mm ] rotor [ mm ] ®...
Page 31
Rotors and accessories Table 3: Adapter (2) * max. tube length with aerosol-tight cap max. tube tubes color order no. Adapter and accessories for dimensions diameter rectangular buckets 7500 6446 d x length / * [ mm ] rotor [ mm ] ®...
Page 32
Rotors and accessories Table 3: Adapter (3) * max. tube length with aerosol-tight cap max. tube Adapter and accessories for tubes color order no. dimensions diameter ® d x length / * BIOshield Rotor 7500 6435 [ mm ] rotor [ mm ] ®...
Page 33
Rotors and accessories Table 3: Adapter (4) Adapter for max. tube dimensions tube capacity number color order no. Micro Liter Rotor 7500 3332 x length [ mm ] [ ml ] per set Reduction sleeve PCR 6.2 x 20 grey 7600 3750 Reduction sleeve 8 x 43.5...
Page 34
Rotors and accessories Table 3: Adapter (5) max. tube dimensions number per number color order no. Adapter for Fixed-Angle Rotor x length adapter 7500 3334 [ mm ] rotor 1.5 ml micro tubes 11 x 51 nature 7600 2905 3.5 ml 11 x 95 nature 7500 3091...
Rotors and accessories Handling the rotor On swinging bucket rotors, at regular inter- vals, apply a light coating of lubricant to the rotor body trunnion pins and to the corre- sponding mating surfaces on the buckets! Lubricant 7000 6692 is supplied with the centrifuge. Swinging Bucket Rotor 7500 6445 (7500 6434 with locking mechanism) The various swinging buckets are split up into weight...
Page 36
Rotors and accessories Tissue culture bucket 7500 6497 Deepwell plates can also be inserted in the carrier without using plate holder. Large volume tissue culture tubes of 175 to 250 ml capacity, available from various manufacturers, can be Insert the individual plate holders as shown in the illus- used directly.
Page 37
Rotors and accessories ® BIOshield Rotor 7500 6435 The bucket set is a permanent part of the rotor and must not be interchanged with any different rotor. Do not run the rotor without the rotor cover installed. The rotor cover is installed and removed by pushing down and turning the cover locking knob.
Page 38
Rotors and accessories ® The following examples are intended to illustrate the Highplate Rotor 7500 6444 different estimates of rotor life for various typical every- Do not run the rotor without the rotor cover installed. day use: Profile of use Max.
Page 39
Rotors and accessories ® Handling micro plates Highconic - Rotor 7500 3057 Remove the appropriate plate holder from the carrier body for loading and unloading ® micro plates in the Highplate Rotor 7500 6444. Before loading, ensure that the rubber bottom is placed in the cut-outs of the bottom of the plate holder.
Rotors and accessories Aerosol-tight operation Use the special lubricant 7600 3500 only to grease the seals ! Aerosol-tight rotors and tubes are only to be opened in an approved safety Spare parts are delivered with the rotor or may be or- work bench when centrifuging danger- dered separately.
Page 41
Rotors and accessories ® BIOshield -Rotor Closing the aerosol-tight bio-containment ® Highplate -Rotor After greasing the seal, turn the cover until it sits lightly on the bucket. The aerosol-tight bio-containment of air To achieve uniform seal, turn the cover clockwise by vessel rotors is only warrented in a 1½...
Page 42
Rotors and accessories ® Closing Highconic - Rotor aerosol-tightly The hexagon wrench of the pliers should be used as a support tool to fasten and loosen the lid of the fixed angle rotor in order to achieve secure closing (insert the hexagon through the hole in the screw cap).
Rotors and accessories • Shaking the bucket releases the carbon dioxide of Checking of aerosol-tight bio-containment the water, and an excessive pressure is built up. The checking of the rotor type and bucket was done • Leaks are recognized by humidity release and according to the dynamic microbiological test proce- audible disinflation of gas mix.
Operation Operation Switching on the centrifuge Locate the main power switch on the right-hand side of the front panel, and set it to the ON (I) position. For a Actuating the lid couple of seconds the following reading appears in the control panel: Opening the lid Press the "open lid"...
Operation 4. The rotor must glide freely down the collet chuck Installing the rotor until it hits the lower stop. 5. If you have positioned the rotor correctly, you can Improper or improperly combined tighten the collet chuck easily using the hexagon accessories may cause severe dam- wrench supplied.
Operation If you wish to centrifuge samples that together with the Loading the rotor adapters exceed the maximum permissible load, you Maximum loading must either reduce the sample volume or calculate the permissible speed n according to the following for- perm Overloading can result in destruction mula:...
Operation Maximum permissible load difference Filling the centrifuge tubes The smaller the imbalance of the centrifuge, the better the separation effect, because as Check carefully whether your tubes imbalance is minimized, so is the resultant are approved for the respective RCF vibration that could affect separation quality.
Operation Inserting the centrifuge tubes Fixed-angle rotors: The rotor must be loaded symmetri- cally. Failure to do so can cause rotor imbalance, which may lead to noisy operation, affect separation quality, or result in imbalance detection shut- down, as well as introduce significant detrimental wear to the motor and drive system.
Page 50
Operation Swinging bucket rotors: Improper loading These examples are to be applied to the other rotors in an analogous manner! Proper loading...
Operation Bucket selection for swinging bucket Entering parameters rotors Deceleration curves When running a swinging bucket rotor, the automatic The Legend™ offers 3 deceleration profiles for optimal rotor identification feature will recognize the rotor body centrifuging of samples and gradients. Please consult and not determine which bucket or carrier is installed.
Operation Selecting speed By pressing the adjusting knob once again the set value mode may be left before the The centrifuge speed can be set to a minimum of 300 end of the waiting period, enhancing the rpm and to a maximum of 15 000 rpm (depending on input the rotor).
Operation More about the RCF value Selecting run time The relative centrifugal force (RCF) is given in multi- There are two time modes: standard and extended. ples of the earth gravity g. It is a dimensionless number In the standard time mode you can select a run time that allows one to compare the efficiency of separation between 1 min and 9 h 59 min or continuous operation or sedimentation of diverse instruments, since it is...
Operation 3. After the required value has been achieved the Extended time mode decimal place is flashing several seconds and the As an option there is the possibility to change to the display changes to the continuous display. The run extended time mode.
Operation Selecting the temperature Starting the centrifuge You can select the temperature in the range of -9 °C to Once the rotor is properly installed, the main switch +40 °C. turned on and the lid is closed, you can start the centri- fuge.
Operation Imbalance display Stopping the centrifuge If rotor imbalance is detected, shortly after the rotor Stopping with preset run time reaches 300 rpm, the message "bAL " will appear in the Normally the run time has been selected, and all you speed display.
Operation Temperature control during standby ”Quick Run” Temperature control becomes active once the rotor For short-term operation the Legend™ is equipped has been identified. This is the case after a centrifuga- with a "Quick Run" function tion run exceeding 300 rpm. At standby the display Short-term centrifugation is started by pressing the reads "End".
Operation Removing the rotor Audible alarm 1. Open the centrifuge lid. Accompanying all error messages, a warning signal is given out which only is silenced upon pressing any key. 2. Remove the rotor cover (on applicable rotors). You have the option of signaling the end of a run 3.
Operation Turning the centrifuge off By switching the main switch into "0“ position the cen- trifuge is turned off. The main power switch should be turned off after a complete centrifugation run. Without motor deceleration, it takes much more time until the rotor comes to a halt.
Clean them with mild agents of pH values ranging from 6 to 8. If you intend to use cleaning agents or disinfection procedures not rec- For other cleaning agents please consult KENDRO ommended by the manufacturer, you Services! have to make sure by consulting the...
Maintenance and care Disinfection If a centrifuge tube containing infectious material leaks During cleaning liquids and espe- during a run, you have to disinfect the centrifuge im- cially organic solvents should not mediately. come into contact with the drive shaft and the ball bearing. Organic solvents may decompose Infectious material could enter the centri- the lubricant of the motor bearing.
Page 63
7. Dispose of the disinfectant according to valid regu- lations. 8. Aluminum rotors have to be treated with anticorro- For other disinfectants please consult KENDRO sive protective oil subsequently. Services! • You may disinfect the rotor and the accessories as Disinfection with bleaching lye described in the following section.
Maintenance and care Decontamination For general radioactive decontamination, use a solu- Chemical additives to the steam are not tion of equal parts of 70% ethanol, 10% SDS and wa- permitted. ter. Follow this with ethanol rinses, then de-ionized water rinses, and dry with a soft absorbent cloth. Dis- pose of all washing solutions in appropriate radioactive Never exceed the maximum permis- waste containers!
• the rotor fastening and the drive shaft thorized by KENDRO Defective material is exchanged. • the required maintenance and care procedures are KENDRO offers inspection and service contracts cov- carried out regularly. ering it. Inspection costs are charged as flat-rate con- tracts.
Troubleshooting Troubleshooting Proceed as follows: 1. Make sure that the rotor is at a stand still Emergency lid release (observe through window in the cover). In case of a power failure the lid could not be opened normally using the electrical lid unlocking mechanism During a power failure it is impossible To permit unloading in this case, the centrifuge is to lock the lid once the emergency lid...
Page 68
Troubleshooting 4. After finished, push the cord back into the instru- ment and reinserting the plastic plug. Once the power is restored, you can connect the in- strument to the main supply and turn it on. Following the self test of the centrifuge, the lid may be closed and locked with the motor.
1. Is the main plug connected to the main socket? deceleration. The lid 2. Check the main connection. cannot be opened. 3. If the main connection is OK, contact KENDRO service. Displays fail briefly. The drive stops suddenly. Brief interruption of main supply The rotor stops without 1.
Page 70
3. Check whether a broken tube or damage to the rotor was responsible for imbalance switch actuation. 4. Check that the trunnions of the swinging bucket rotor have been properly lubricated. If you cannot locate and solve the problem yourself, contact the KENDRO service.
Page 71
Troubleshooting Error Symptom Possible causes and corrective measures Message "rotor" appears Rotor decelerates with Set speed exceeds permissible maximum speed for the in display. delayed deceleration. rotor. (The same holds for RCF setting) A) For about 15 sec. the display shows alternately "rotor" and the maximum permissible speed or RCF for the installed, after the rotor identification.
Page 72
E-15 Rotor stops with Temperature measurement error. deceleration to standstill. Switch the instrument off and on again. If the problem Instrument cannot be persists, contact KENDRO service. E-16 operated. E-17 Rotor stops with Max speed for the rotor identification exceeded.
Page 73
Troubleshooting Error Symptom Possible causes and corrective measures E-19 Instrument stops with No rotor present or rotor identification impossible. deceleration to standstill A) Check whether a certified rotor is inserted. after short starting-up. B) Please take care of the readability of the inscription of the swinging bucket rotor cross installed.
Page 74
E-22 Rotor stops without brake Error in speed measurement to standstill. Switch the instrument off and on again. If the error persists, Instrument cannot be contact KENDRO service E-23 operated. E-24 Instrument cannot be Wrong status information from lid lock.
Page 75
1. Switch instrument off and on again using the main switch. 2. Open the lid. 3. Check whether the rotor can turn freely. If you cannot clear the malfunction, contact KENDRO service. E-30 Rotor stops without brake Control voltage breaks down.
Page 76
Check and clean ventilation slots if necessary. After about 60 min you can restart the instrument. Observe the max. permissible environmental temperature! If the error persists, contact KENDRO service. E-34 Rotor stops without brake Overvoltage in the intermediate circuit.
Page 77
Open the lid and check the load! Instrument cannot be Significant imbalance caused by missing bucket operated. Tubes or adapter do not fit (rotor is rubbing the chamber or the cover) Micro plates carriers on the motor cover If the error persists, contact KENDRO service.
Troubleshooting Subsequently, the following readings will be dis- Contacting Kendro Service played for 5 seconds each: Should you require our Service, please advise us of (numbers are examples) the catalog and serial number of your instrument. You will find the pertinent information at the specifications, Software version keyboard near the socket for the main plug.
Technical Data Technical Data Features specification - indoor use Ambient conditions - maximum elevation 2000 m (6562 ft) above sea level - max. relative humidity 80 % up to 31°C (88°F), linearly decreasing down to 50 % relative humidity at 40°C (104°F). Ambient temperature allowed +2 °C to +35 °C (36°F to 95°F)
Page 80
Technical Data Features specification Dimension (H x W x D) Legend™ T 362 mm x 551 mm x 666 mm ( 14.3 x 21.7 x 26.2 inches ) Legend™ RT 362 mm x 733 mm x 666 mm ( 14.3 x 28.7 x 26.2 inches ) Weight without rotor Legend™...
Technical Data Electrical connections / fuses Order no. Voltage Frequency Nominal Power Fuse protection of Fuse current consumption instrument protection of building safety fuse * thermic excess current release Legend™ T 120 V 60 Hz 10.2 A 900 W 15 A 15 AT 7500 4366 Legend™...
Index changing settings during run · 52 cleaning · 57 closing the lid · 41 collet chuck · 42 adapter · 26 common glass or plastic centrifuge tubes · 4 aerosol-tight operation · 36 conditions of warranty · 61 aerosol-tightness contamination test ·...
Page 97
opening the lid · 41 blockage · 65 operation Lid lock with safety check · 8 short-time · 53 lid opening · 41 organic solvents lid release not allowed for cleaning · 58 emergency · 63 lid unlocking mechanism manual · 63 limited life time ·...
Page 98
Run time selection · 49 radius of centrifugation for calculation of RCF value · 49 safety instructions · 3 maximum · 51 safety measures · 3 RCF display safety standards · 5 and rotor identification · 48 safety zone · 3, 13 RCF selection ·...
Page 99
speed of centrifugation t-set for calculation of RCF value · 49 time menu · 50 Speed selection, · 10 tube starting the centrifuge · 51 breakage with infectious material · 58 stopping Turning the centrifuge off · 55 manually · 52 with continuous operation ·...
Page 101
Kendro Laboratory Products International Sales · Asheville, NC · USA · Tel. +1 828-658 2711 · Fax +1 828-645 9466 · info@kendro.spx.com North America Kendro Laboratory Products · Asheville, NC · USA · Tel. +1 800 -879 7767 · Fax +1 828-658 0363 · info@kendro.spx.com Internet http://www.kendro.com...
Need help?
Do you have a question about the Sorvall Legend T and is the answer not in the manual?
Questions and answers