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Summary of Contents for Magus BIO 270T
- Page 1 M AG US B IO 2 70T B I OLO G ICA L MI C ROS C O P E U S E R M A N U A L...
- Page 2 928 E 124th Ave. Ste D, Tampa, FL 33612, USA +1 813 468-3001 contact_us@levenhuk.com Levenhuk Optics s.r.o. (Europe) V Chotejně 700/7, 102 00 Prague 102, Czech Republic +420 737 004-919 sales-info@levenhuk.cz Magus® is a registered trademark of Levenhuk, Inc. © 2006–2024 Levenhuk, Inc. All rights reserved. www.levenhuk.com 20241031...
- Page 3 Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures.
- Page 4 12. Do not touch the lens surfaces with your fingers. Use a brush and special lens-cleaning solution to keep the lenses clean. 13. Bulb installation. This microscope employs LED bulbs as a light source. The bulbs should be replaced by the equipment vendor or in a qualified service center. If you replace it yourself, the illumination function may be impaired.
- Page 5 Phase-contrast device Polarizer/analyzer set Using the eyepiece with a scale Using the camera Using the calibration slide with a camera 6 TROUBLESHOOTING 7 SCOPE OF DELIVERY 8 CARE AND MAINTENANCE Replacing the bulb and power adapter Maintenance 9 MAGUS WARRANTY...
- Page 6 MAGUS Bio 270T Biological Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation.
- Page 7 LCD screen Zernicke condenser Phase-contrast technique* Centering telescope Set of phase-contrast objectives Oil darkfield condenser (NA 1.3–1.26) Darkfield technique* Dry darkfield condenser (NA 0.7–0.9) Fluorescence technique* Reflected light illuminator Polarizer/analyzer set*, installation Polarizer (on the collector) method Analyzer – in the slot above the revolving nosepiece AC power supply Voltage 100–240V...
- Page 8 Fig. 1. MAGUS Bio 270T Biological Microscope. View from the right 11. XY stage control knob 1. Eyepieces 6. Condenser centering screws 2. Eyepiece tubes 7. Condenser locking screw 12. Stage holder with a condenser mount 3. Revolving nosepiece 8. Coarse focusing tension adjusting knob 13.
- Page 9 Fig. 2. MAGUS Bio 270T Biological Microscope. View from the left 11. Condenser focus knob 1. Analyzer slider insertion slot 6. LCD screen 2. Stage 7. Lighting control knob 12. Stand 3. Abbe condenser 8. Coarse focusing lock knob 13. Microscope head 4.
- Page 10 Fig. 3. MAGUS Bio 270T Biological Microscope. Rear view 1. Allen wrench 3. ON/OFF switch 5. Power adapter 2. Power cord 4. Holder for the power adapter 6. Power connector and power cord...
- Page 11 COMPONENTS STAND The stand 12 (Fig.2) is a one-piece structure with the base. The base has Y-shaped stable ergonomic design. There is a revolving nosepiece 3 (Fig. 1) and a collector 5 (Fig. 2) fixed on the stand. Inside the stand is the focusing mechanism. A stage holder with a condenser mount 12 (Fig.
- Page 12 A C-mount adapter is installed in the trinocular tube 14 (Fig. 1) to fix the camera. The camera is used to transmit the image to a computer screen or monitor/TV. The beam splitting ratio (%) for eyepieces/trinocular tube: 50/50. EY EPI EC ES The microscope kit includes eyepieces 1 (Fig.
- Page 13 Plan 100x/1.25 oil oil immersion 100x 1.25 0.17 white ∞/0,17 Plan 100x/1.10W water 100х 1.10 0.16 0.17 white ∞/0.17 immersion The 50x, 60x and 100x objectives have a spring-loaded mount to prevent mechanical damage to the front lens and the object. If objectives are damaged, we recommend repairing them in the service center.
- Page 14 3 UNPACKING AND ASSEMBLING The assembly procedure is given in Fig. 4. Fig. 4. Assembling the microscope 1. Remove the microscope from the package. 2. Check the scope of delivery using Section 7 of the User Manual. 3. Inspect the microscope and its components for damage. 4.
- Page 15 5. Insert the stage, aligning it with the center of the round holder. Tighten the screw until the stage is fixed. Fig. 6 (a, b). Installing the stage 6. Insert the microscope head into the mounting hole of the stand and tighten the screw. Place the C-mount adapter into the trinocular tube and secure it with a screw.
- Page 16 10. Make sure that all the components are securely and safely mounted. 11. Check and sort the supplied accessories and tools in the correct order. Keep them in proper order to avoid confusion. 4 BRIGHTFIELD OBSERVATION PROCEDURE SWITCHING ON T H E I LLU MI N ATI O N Before switching on the ON/OFF switch, make sure that the input voltage of the microscope power supply matches the local mains voltage.
- Page 17 Brightness adjustment The knob 1 adjusts the brightness when the screen shows an image like the one shown on the left. By rotating the knob counterclockwise, you decrease the brightness. By rotating the knob clockwise, you increase the brightness. Color temperature adjustment You can adjust the color temperature with the knob 1 when you see an image on the screen like the one shown on the right.
- Page 18 Adjusting the revolving nosepiece The revolving nosepiece is coded by default, and usually users do not need to change them. If you need to make changes, you should do as follows: – select Set on the LCD screen – screw in the objective and introduce it into the optical path –...
- Page 19 Fig. 14. Focusing on the specimen A DJ USTI NG THE EY E PI ECE T UB ES Use the diopter adjustment ring to compensate for the observer's ametropia. The adjustment range is ±5 diopters. Set the diopter adjustment 1 on both tubes to the middle position so that "0"...
- Page 20 – Carefully moving the condenser up and down by rotating the condenser focus knob 1, place the condenser into the working position. In this position, the edges of the octagon-shaped image of the closed field diaphragm are sharp and the diffracted blue-green color at the edge of the diaphragm is directed beyond the edge of the diaphragm and not into the field of view.
- Page 21 Place the 100х oil objective into the optical path. Observing the gap between the objective and the slide from the side, raise the stage slowly using the coarse focus knob until the drop of oil on the slide comes in contact with the objective lens.
- Page 22 For good darkfield performance, specimens with a slide thickness of no more than 1.2mm and a coverslip thickness of no more than 0.17mm should be used. When using the darkfield technique with the immersion objective having a high aperture, the objective captures not only the light scattered by the specimen particles, but also the direct rays that create a light background and deteriorate the image contrast.
- Page 23 Place the calibration slide on the stage instead of the specimen with the scaled side facing up. Using the scale of the calibration slide, calibrate the eyepiece scale for each objective that will be used for measurements. To do this, bring the image focus of the calibration slide scale into sharp focus in the plane of the eyepiece scale and rotate the eyepiece in the tube, setting the strokes of both scales in parallel.
- Page 24 U SING THE CAL I B R ATI O N SLI D E WI T H A CA M ER A The calibration slide (stage micrometer) is used to calibrate the image analysis software for measurements in actual units. In the calibration mode, you should capture an image of the micrometer scale with every objective magnification and indicate the known distance.
- Page 25 OPT ICS AND IM AGE REP RODUCTION The objective is damaged Have the objective repaired by a qualified technician or replaced Inappropriate coverslip thickness Use the specimen with the coverslip of standard thickness (0.17mm) The specimen is mounted upside Place the specimen with the coverslip down facing up There is immersion oil on the front...
- Page 26 7 SCOPE OF DELIVERY The scope of delivery (Table 4) Component Note MAIN COMPONENTS Stand (including a transmitted light illuminator built into the base, a focusing mechanism, and an LCD screen) Trinocular microscope head Stage holder with a condenser mount Revolving nosepiece Mounted on the stand Stage...
- Page 27 PlanF S-Apo 40x plan semi-apochromatic fluorescent infinity-corrected Optional objective, parfocal height: 60mm PlanF S-Apo 100x (oil) plan semi-apochromatic fluorescent infinity-corrected Optional objective, parfocal height: 60mm C-mount camera adapter On eyepieces Camera Calibration slide Optional Monitor Optional Монитор Optional ACCESSORIES AND SPARE PARTS Head locking screw Allen wrench Trinocular tube dust cap...
- Page 28 6. Periodic inspection: the microscope should be regularly inspected and serviced to maintain its performance. MAGUS WARRANTY MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product.
- Page 30 www.magusmicro.com...
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