Related Manuals for Magus LUM V500
Summary of Contents for Magus LUM V500
- Page 1 M AG US LUM V 5 0 0 I NVE RT ED FLU O RES C EN C E M I C RO S C OP E U S E R M A N U A L...
- Page 2 928 E 124th Ave. Ste D, Tampa, FL 33612, USA +1 813 468-3001 contact_us@levenhuk.com Levenhuk Optics s.r.o. (Europe) V Chotejně 700/7, 102 00 Prague 102, Czech Republic +420 737 004-919 sales-info@levenhuk.cz Magus® is a registered trademark of Levenhuk, Inc. © 2006–2024 Levenhuk, Inc. All rights reserved. www.levenhuk.com 20241120...
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Page 3: Safety Precautions
Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures. - Page 4 11. Bulb installation: – Do not touch the glass surface of the bulb with your bare hands. When installing the bulb, wear gloves or wrap the bulb with a cotton cloth. – Use a clean cotton cloth moistened with alcohol-based disinfectant to wipe dirt off the surface of the bulb.
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Page 5: Table Of Contents
7 USING OPTIONAL EQUIPMENT Using the eyepiece with a scale Using the camera Using the calibration slide with a camera 8 TROUBLESHOOTING 9 SCOPE OF DELIVERY 10 CARE AND MAINTENANCE Replacing the bulb and the fuse Maintenance 11 MAGUS WARRANTY... -
Page 6: Description Of The Microscope
MAGUS Lum V500 Inverted Fluorescence Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation. -
Page 7: Microscope Kit
Revolving nosepiece 6 objectives Optical design Infinity plan achromatic, parfocal distance: 45mm; long focal length Objectives PLL 10х/0.25/4.3; PLL 20x/0.40/8.0; PLL 40x/0.60/3.5 (spring-loaded); magnification, x/aperture/working phase-contrast: PLL 10x/0.25/4.3 PHP2; PLL 20x/0.40/8.0 PHP2; distance, mm PLL 40x/0.60/3.5 PHP2 (spring-loaded); PL 4x/0.10* Numerical aperture: 0.6 Condenser Working distance: 55mm. - Page 8 Fig. 1. MAGUS Lum V500 Fluorescence Microscope. View from the left 1. Eyecups 11. Mercury lamp adjustment knobs 2. Eyepieces 12. Collector adjustment knob 3. Stand 13. Mercury lamphouse 4. Fluorescence filter assembly 14. Revolving nosepiece 5. ON/OFF switch 15. Objectives 6.
- Page 9 Fig. 2. MAGUS Lum VD500 Fluorescence Microscope. View from the right 1. Trinocular tube 11. Stage 2. Interpupillary distance adjustment ring 12. Dish holder 3. Eyepiece tubes 13. Locking knob for the condenser 4. Knob for switching the light path to the trinocular tube 14.
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Page 10: Microscope Parts
2 MICROSCOPE PARTS STAND The stand has stable ergonomic design. Parts attached to the microscope stand 3 (Fig. 1): – revolving nosepiece 14 (Fig. 1) with objectives – stage 11 (Fig. 2) – condenser mount 16 (Fig. 1) – transmitted light illuminator 17 (Fig. 1) –... -
Page 11: Eyepieces
The interpupillary distance is adjusted by rotating the eyepiece tubes 2 (Fig. 2) in the range of 48–75mm. The distance between the eyepieces matching the observer's interpupillary distance is marked on the adjustment scale 2 (Fig. 2). For convenience, the microscope head is inclined at 45°. Microscope head magnification: 1x. -
Page 12: Transmitted Light Illuminator
T RANS M ITTED LI GHT I LLUM INATO R The microscope illuminator allows for setting up Köhler illumination. The illuminator 17 (Fig. 1) is fixed on the microscope stand. The light source – 12V/30W halogen bulb – is adjusted to the optical path and collector lens using three screws 16 (Fig. -
Page 13: Mercury Lamphouse
The fluorescence of the specimen parts in the reflected light, once the light has passed the emission filter, is observed in the wavelength range of 595–700nm, which corresponds to orange-red color. The other three fluorescence filter cubes function in a similar manner. Table 3 shows the specifications of the four fluorescence filters: Excitation wavelength Dichroic mirror wavelength... -
Page 14: Unpacking And Assembling The Microscope
3 UNPACKING AND ASSEMBLING THE MICROSCOPE The assembly procedure is given in Fig. 4. Fig. 4. Assembling the microscope 1. Unpack the microscope and check the scope of delivery using Section 8 of the User Manual. 2. Take out the stand 1 and place it on a stable work table, remove protective packaging and dust cover. 3. - Page 15 Fig. 4c. Installing the mercury lamphouse power supply Use the screwdriver to loosen the screws 1. Open the cover 2. Loosen two screws 5 on the lamp socket 6 and remove the plastic rod. Remove the mercury lamp 4 from the package and insert its ends into the socket rings. Position the center of the lamp in line with two center screws and Phillips screw 7.
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Page 16: Brightfield Observation Procedure
4 BRIGHTFIELD OBSERVATION PROCEDURE SWITCHING ON T HE I LLUM I N ATI O N Before switching on the ON/OFF switch, make sure that the input voltage of the microscope power supply matches the local mains voltage. If not, do not switch on the microscope. Improper input voltage may result in a short circuit or fire. Turn the ON/OFF switch 1 to "–"... -
Page 17: Adjusting The Eyepiece Tubes
ADJ U STI N G THE E YE P I ECE T UB ES Rotate the diopter adjustment ring 1 to find the position, at which the height of both eyepieces will be the same. Adjust the interpupillary distance. Adjust the distance between the eyepieces to your interpupillary distance by rotating the eyepiece tubes 2 around the central axis until you see a single circular image when looking through the eyepieces with both eyes... -
Page 18: Placing The Specimen
PL ACI N G THE SP EC IM EN Choose the appropriate dish holder from the kit based on its shape and size (Fig. 12). Install the dish holder on the stage. Fix the slide 1 or the dish with the studied sample in it. Fig. -
Page 19: Setting Up Köhler Illumination
Adjust the coarse focusing tension. The tension of the coarse focusing knob is adjustable and is preset by the manufacturer for convenient use. If you need to adjust the tension of the coarse focusing, rotate the coarse focusing tension adjusting knob 4. By rotating it clockwise, you loosen the tension, and by rotating it counter-clockwise, you tighten it. -
Page 20: Adjusting The Tension Of The Object Moving Knobs
Remove the mechanical XY attachment from the stage. To do this, use the screwdriver to loosen 3 attachment screws 5 below the stage and remove the attachment. Put the attachment aside – lay it flat on a surface with the handle facing up so that it will not fall or be damaged. -
Page 21: Phase-Contrast Technique
5 PHASE-CONTRAST TECHNIQUE The condenser allows utilizing the phase-contrast technique. Brightfield observations are performed as described above in the user manual. The following instructions apply only to the phase-contrast operation mode. Adjusting the phase-contrast device: 1. Take the 10х phase-contrast objective from the microscope kit and place it into the optical path. Swing the corresponding condenser phase annulus into the optical path by rotating the turret 1 so that number "10"... -
Page 22: Fluorescence Observations
6 FLUORESCENCE OBSERVATIONS Transmitted light observations are performed as described above in the user manual. The following instructions apply only to the reflected light observations using the fluorescence technique. Select the appropriate fluorescence filters to match the dyes applied. In order to prevent the fluorescence from quenching, the specimens should be placed in the optical path 5–10 minutes after switching on the lamphouse power supply, when the mercury lamp becomes fully operational. - Page 23 Switch on the mercury lamphouse power supply. Wait 5-10 minutes for the mercury lamp to be in a steady-state operation mode. Adjust the field diaphragm 1 so that the entire field of view is illuminated. If a smaller field of view is required when using the camera, close the aperture to 60-70% of the field.
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Page 24: Using Optional Equipment
7 USING OPTIONAL EQUIPMENT U SING THE EYEP I ECE W I TH A SCALE The eyepiece with a scale or reticle can be used to make comparative analysis of the linear dimensions of the individual components of an object. The scale is installed in the plane of the field diaphragm of the 10x eyepiece. - Page 25 To enable the camera: – Loosen the attachment screw 1 of the side camera port. Remove the dust cap 2. – The microscope kit includes a C-mount adapter. Connect the camera to the adapter. – Fit the camera into the trinocular tube and secure it with the screw 1. –...
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Page 26: Using The Calibration Slide With A Camera
– Move the specimen and check whether the image of the specimen on the monitor or display screen moves in the same direction as the specimen does. If the image moves in another direction, you should adjust the camera position. Loosen the lock screw 1, rotate the camera to make the displayed image direction in line with the direction of stage movement, then secure the screw. -
Page 27: Mechanical Components
The objective is damaged Have the objective repaired by a qualified technician or replaced Inappropriate dish bottom thickness Use the glassware with a standard bottom thickness (1.2mm) Poor image quality (low resolution, poor contrast) The aperture diaphragm is opened too Adjust the opening to match the numerical wide aperture of the objective used... -
Page 28: Scope Of Delivery
SCOPE OF DELIVERY The scope of delivery (Table 5) Component Note MAIN COMPONENTS Stand with a built-in power supply, transmitted light source, focusing mechanism, condenser mount, and trinocular tube Fluorescence filter assembly Mercury lamphouse Trinocular microscope head Revolving nosepiece Mounted on the stand Fixed stage Mounted on the stand Mechanical XY stage attachment... -
Page 29: Care And Maintenance
10 CARE AND MAINTENANCE REPL ACIN G T HE BU LB AND THE FUSE Before replacing the bulb or fuse, turn the ON/OFF switch to "0" position (off). Unplug the power cord from the power outlet. Wait about 10 minutes for the bulb to cool down. 1. -
Page 30: Maintenance
Fig. 30. Replacing the mercury lamp – Re-install the cover on the lamphouse. An "up" arrow indicates the top of the cover. Tighten the attachment screws. – Plug the power cord to the mercury lamphouse. Important! It is strictly forbidden to turn on the lamp until the lamp cover is re-installed. Exposure to the uncovered lamp will cause eye and skin burn. -
Page 31: Magus Warranty
11 MAGUS WARRANTY MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product. - Page 32 www.magusmicro.com...
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