Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures.
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BUL B I N STAL LAT IO N ( FO R MO D E LS W IT H HA LO G EN BU L BS ) – Do not touch the glass surface of the bulb with your bare hands. When installing the bulb, wear gloves (supplied) or wrap the bulb with a cotton cloth.
CONTENTS 1 D ES C RI PT I O N OF TH E M IC ROSC OP E Purpose Specifications Microscope kit 2 M I C RO S C O P E PARTS Stand Focusing mechanism Microscope head Eyepieces Revolving nosepiece Objectives Condenser Stage...
MAGUS Bio D250 LCD Biological Digital Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation.
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Coaxial coarse & fine focusing knobs on both sides Focusing range: 21mm Coarse focusing travel: 39.8mm/circle Focusing mechanism Fine focusing scale value: 2μm Coarse focusing tension adjusting knob Coarse focusing lock knob Halogen bulb Light source 3W LED 12V/30W, G4 base Phase-contrast turret condenser Фазово-контрастное...
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Mount type C-mount Body material Metal Power supply 12V/1A AC power adapter Monitor Type of matrix Screen diagonal, inch 13.3 Screen resolution, pix 1920x1080 (Full HD) Aspect ratio 16:9 Brightness, cd/m2 Number of displayed colors 16.7m Contrast ratio 1000:1 Horizontal/vertical viewing angle, ° 178/178 Viewable screen size (WxH), mm 295x165...
M ICROS COPE K IT This microscope kit includes the following main components: – stand with a built-in power supply, transmitted light source, focusing mechanism, stage, condenser, and revolving nosepiece – microscope head – set of objectives and eyepieces – digital camera –...
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Fig. 1. MAGUS Bio D250 LCD Microscope. View from the right Eyepieces Brightness 10. X/Y stage control knobs 14. Monitor adjustment ring Eyepiece tubes 11. Head locking screw 15. Camera Coarse focusing tension Revolving nosepiece 12. Trinocular tube adjusting knob Objectives 13.
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Fig. 2. MAGUS Bio D250 LCD Microscope. View from the left 1. Diopter adjustment ring 5. Condenser mount 9. Fine focusing knob 13. Microscope head 2. Analyzer slider 6. Field diaphragm ring 10. Condenser focus knob 14. Beam splitter lever insertion slot 7.
M I C RO SC OP E PA RTS STAND The stand 12 (Fig.2) is a one-piece structure with the base. The base has Y-shaped stable ergonomic design. Parts attached to the microscope stand: – revolving nosepiece 3 (Fig. 1) with objectives 4 (Fig. 1) –...
For convenience, the microscope head is inclined at 30°. Microscope head magnification: 1x. Eyepiece diameter: 30mm. The eyepiece diopter adjustment is intended to compensate for the observer's ametropia. It is located on the left eyepiece tube – ring 1 (Fig. 2). The Gemel head design allows for the 360°...
The camera is powered via a 12V/1A AC power adapter. MONITO R The monitor is designed to use a visualization system of the MAGUS microscope. It is connected to the camera mounted on the microscope to display the real-time images.
Remove the microscope from the package. Check the scope of delivery in item 7 of the User Manual. Inspect the microscope and its components for damage. Loosen the head locking screw 3, turn the microscope head 2 with the tubes toward the specimen stage and fix it with the head locking screw on the stand 1.
PL AC IN G THE SP EC IM E N Place the specimen 1 on the stage. Adjust the image by moving the stage control knobs 2 and 3 so that the observed section of the specimen is directly under the objective.
ADJ U STI N G THE E YE P I ECE T UB ES Use the diopter adjustment of the left eyepiece tube to compensate for the observer's ametropia. Start by setting the diopter adjustment to the zero. To do this, rotate the ring 1 to adjust the scale “0” to the indicator 2. While looking through the right eyepiece (with your left eye closed), bring the specimen into focus.
SE TTI N G U P KÖ H L ER IL LU MI NAT IO N In the light optical microscope, the image quality depends equally on the optics and on the illumination system, so adjusting the illumination is an important preparatory step. The illumination system affects the image resolution, comfort during long observation, and photo quality when using digital cameras.
When you switch to the objectives of other magnifications, do not change the height of the condenser, only adjust the opening of the field and aperture diaphragms. While adjusting the illumination, you should keep in mind that changing the size of the field diaphragm only affects the size of the illuminated field.
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Dust cap screw Dust cap Trinocular tube centering screw Beam splitter lever Fig. 10. Using the camera To enable the camera: – Loosen the attachment screw 1. Remove the dust cap from the trinocular tube. – The microscope kit includes a C-mount adapter. Connect the camera to the adapter. –...
– Pull out the beam splitter lever 4 to the camera position. Look at the specimen on a monitor or display screen and make sure that the image of the target is in the center of the field of view. If the image deviates from the center of the field of view, adjust three centering screws 3 on the trinocular tube to move the target towards the center.
USI NG O PT ION AL EQU IP M E N T DAR KF IE LD CO N D E NSE R The optional darkfield condenser is used in the darkfield microscopy technique. This technique is used to obtain the image of unstained transparent weakly absorbing samples and therefore invisible when observed in the bright field.
PHASE- CO NTR AST DE V I CE The phase-contrast device is designed for the study of low-contrast objects which are invisible in transmitted light of the brightfield microscopy. The phase-contrast technique allows for the observation of unstained low-contrast objects, colorless transparent specimens and living microorganisms.
We recommend entering the obtained data in a size chart. Objective magnification Eyepiece division value Using these data to determine the actual linear size of the specimen, you just need to count the number of divisions of the eyepiece scale aligned with the area of the specimen being measured, and multiply this number by the scale division value specified in this table.
T RO U B L ESH OOT ING Potential problems and remedies are given in Table 3. Table 3 Problem Cause Remedy ELECT RI CAL C OMPONENTS The ON/OFF switch is off Switch on the ON/OFF switch The fuse has blown Replace the fuse The bulb is burned out Switch off the power supply.
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The objective is damaged Have the objective repaired by a qualified technician or replaced Inappropriate coverslip thickness Use the specimen with the coverslip of standard thickness (0.17mm) The specimen is mounted upside Place the specimen with the coverslip down facing up There is immersion oil on the front Remove immersion oil from the front lens lens of the dry objective (most often...
SCO P E O F DEL IVE RY The assembly diagram is given in Fig. 16. The scope of delivery is listed in Table 4. Camera C-mount adapter Eyepice Eyepiece with a scale Revolving nosepiece Analyzer Objective Darkfield slider Polarizer Condenser Fig.
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Table 4 Component Note D250T LCD D250TL LCD M I CROS C OP E M AI N C O M PO NE N TS Stand (with the transmitted light illuminator, power source and focusing mechanism built into the base) ICO Infinitive trinocular microscope head Revolving nosepiece Mounted on the stand Stage...
M ON ITO R Monitor HDMI cable AC power adapter DC/DC Type-C adapter Fasteners (Allen wrench and screws) CA R E A N D M AIN T EN AN C E RE PL ACING T HE B ULB A N D T HE FU SE Before replacing the bulb or fuse, turn the ON/OFF switch to "0"...
MAG U S WAR R A NT Y MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product.
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