Magus BIO VD300 User Manual

Biological inverted digital microscope

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M AGUS B I O V D 3 00
B I OLOG I CAL IN V ERT ED D I G I TA L M I C ROS C O P E
U S E R M A N U A L

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Summary of Contents for Magus BIO VD300

  • Page 1 M AGUS B I O V D 3 00 B I OLOG I CAL IN V ERT ED D I G I TA L M I C ROS C O P E U S E R M A N U A L...
  • Page 2: Safety Precautions

    Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures.
  • Page 3 10. Do not touch the lens surfaces with your fingers. Use a brush and special lens-cleaning solution to keep the lenses clean. 11. Bulb installation. This microscope employs LED bulbs as a light source. The bulbs should be replaced by the equipment vendor or in a qualified service center. If you replace the LED yourself, the illumination function may be impaired.
  • Page 4: Table Of Contents

    Using the camera 5 PHASE-CONTRAST TECHNIQUE 6 USING OPTIONAL EQUIPMENT Eyepiece with a scale Using a calibration slide with a camera 7 TROUBLESHOOTING 8 SCOPE OF DELIVERY 9 CARE AND MAINTENANCE Replacing the bulb Replacing the fuse Maintenance 10 MAGUS WARRANTY...
  • Page 5: Description Of The Microscope

    MAGUS Bio VD300 Biological Inverted Digital Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation.
  • Page 6 Windows 8/10/11 (32bit and 64 bit), Mac OS X, Linux, up to 2.8GHz Intel Core 2 System requirements or higher, minimum 2GB RAM, USB 3.0 port, CD-ROM, 17” or larger display Software MAGUS View Mount type C-mount Body material Metal...
  • Page 7: Microscope Kit

    Weight without package 14.3kg Weight with package 18.3kg * Not included in the kit, available on request. ** The magnification of the microscope can be increased by using additional (optional) eyepieces and objectives. The manufacturer reserves the right to make changes to the product range and specifications without prior notice. M ICROS COPE K IT This microscope kit includes the following main components: –...
  • Page 8 Fig. 1. MAGUS Bio VD300 Microscope. View from the left 1. Eyepieces 8. Revolving nosepiece 2. Microscope head 9. Objectives 3. Coarse focusing tension adjusting knob 10. Illuminator 4. Coarse focusing knob 11. Camera locking screw 5. Fine focusing knob 12.
  • Page 9 Fig. 2. MAGUS Bio VD300 Microscope. View from the right 8. Stage 1. Eyepiece tubes 2. Head locking screw 9. Aperture diaphragm control 3. Coarse focusing lock knob 10. Phase-contrast slider 4. Coarse focusing knob 11. Filter 5. Fine focusing knob 12.
  • Page 10: Microscope Parts

    2 MICROSCOPE PARTS STAND The stand has stable ergonomic design. Parts attached to the microscope stand 7 (Fig. 1): – revolving nosepiece 8 (Fig. 1) with objectives – stage 8 (Fig. 2) – condenser 12 (Fig. 2) – lamp house 10 (Fig. 1) –...
  • Page 11: Eyepieces

    A camera (video-eyepiece) or an imaging system with a monitor is installed in the trinocular tube 12 (Fig. 1) of the microscope head using a C-mount 1x adapter. The light path splitting ratio: 50% trinocular tube, 50% eyepieces. EY EPI EC ES The microscope kit includes eyepieces 1 (Fig.
  • Page 12: Stage

    The condenser can be used for the brightfield and phase-contrast techniques. The condenser has a slot for the phase- contrast slider. The phase-contrast slider 10 (Fig. 2) has an open slot and a phase slide for the 10x objective. The iris aperture diaphragm is located under the slot for the phase-contrast slider. The aperture diaphragm is opened by the knob 9 (Fig.
  • Page 13: Brightfield Observation Procedure

    1. Remove the microscope from the package. 2. Check the scope of delivery in item 8 of the User Manual. 3. Inspect the microscope and its components for damage. 4. Take out the microscope head 3, remove the dust cover. Insert the microscope head into the holder 2. Secure the microscope head with the screw 9 using an Allen wrench from the microscope kit.
  • Page 14: Adjusting The Eyepiece Tubes

    ADJ U STI N G THE E YE P I ECE T UB ES Adjust the interpupillary distance. Adjust the distance between the eyepieces to your interpupillary distance by rotating the eyepiece tubes 1 (Fig. 6) around the central axis until you see a single circular image when looking through the eyepieces with both eyes (Fig.
  • Page 15: Focusing On The Specimen

    Fig. 8. Moving the specimen The stage attachment features an XY control system. The control knobs are coaxial – they are located on the same axis. The knob 2 controls longitudinal (Y-axis) movement, the knob 3 controls lateral (X-axis) movement. Moving range: 77mm in Y-axis direction and 112mm in X-axis direction.
  • Page 16: Adjusting The Diopter

    When using high magnification objectives, raise the objective all the way up by rotating the coarse focusing knob and enable the coarse focusing lock knob. After that, focus on the specimen using the fine focusing knob. Adjust the coarse focusing tension. The tension of the coarse focusing knob is adjustable and is preset by the manufacturer for convenient use.
  • Page 17: Calculating The Total Magnification

    CALCU LATI N G T HE TOTAL M AGN IF I CAT I ON The total magnification is the eyepiece power multiplied by the objective power. For example, if the eyepiece is 10х/22mm, and the objective is 40х/0.60, the total magnification of the microscope is 10х40 = 400х.
  • Page 18: Phase-Contrast Technique

    Do it as follows: – While observing the specimen through the eyepieces, find a distinctive point in the field of view (an easily identifiable target, such as point S in Fig. 13а), move the specimen on the stage so that the point is in the center of the field of view, as shown in Fig.
  • Page 19: Using Optional Equipment

    3. Mount the specimen on the stage, focus the microscope. 4. Remove the eyepiece from the tube and insert the centering telescope 2 instead. You will see a dark ring 8 and a light ring 7 in the field of view. 5.
  • Page 20: Using A Calibration Slide With A Camera

    U SING A CAL IBRATI O N SLI D E W I TH A CA ME R A The calibration slide (stage micrometer) is used to calibrate the image analysis software for measurements in actual units. In the calibration mode, you should capture an image of the micrometer scale with every objective magnification and indicate the known distance.
  • Page 21 The objective is damaged Have the objective repaired by a qualified technician or replaced Inappropriate dish bottom thickness Use the glassware with a standard bottom thickness (1.2mm) Poor image quality (low resolution, poor contrast) The aperture diaphragm is opened too Adjust the opening to match the numerical wide aperture of the objective used...
  • Page 22: Scope Of Delivery

    8 SCOPE OF DELIVERY The scope of delivery is listed in Table 4: Component Note MICROSCOPE MAIN COMPONENTS Stand with built-in power supply, transmitted light source, focusing mechanism, and condenser Trinocular microscope head Revolving nosepiece for 4 objectives Mounted on the stand Fixed stage Mounted on the stand Mechanical XY stage attachment...
  • Page 23: Care And Maintenance

    9 CARE AND MAINTENANCE RE PL ACING T HE B ULB This microscope employs LED bulbs as a light source. The bulbs should be replaced by the equipment vendor or in a qualified service center. If you replace the LED yourself, the illumination function may be impaired.
  • Page 24: Magus Warranty

    7. Periodic inspection: the microscope should be regularly inspected and serviced to maintain its performance. MAGUS WARRANTY MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product.
  • Page 26 www.magusmicro.com...

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