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Summary of Contents for Magus BIO D250T
- Page 1 M AG U S BI O D 25 0T | D2 5 0T L BI OLO G I CA L D I G I TA L M I C ROS C O P E USER M ANUAL...
- Page 2 Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures.
- Page 3 10. Do not use any other oily substance instead of proper immersion oil made specifically for the given purpose, as this will degrade the image quality and damage the lenses. 11. Do not touch the lens surfaces with your fingers. Use a brush and special lens-cleaning solution to keep the lenses clean.
- Page 4 C O N T E NTS 1 DES C RI PTI ON OF T HE M IC ROSC OPE Purpose Specifications Microscope kit 2 M I C RO S C O P E PARTS Stand Focusing mechanism Microscope head Eyepieces Revolving nosepiece Objectives Condenser...
- Page 5 MAGUS Bio D250 Biological Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation.
- Page 6 Image size, brightness, exposure Port USB 3.0, 5 Gbps Windows 8/10/11 (32bit and 64 bit), Mac OS X, Linux, up to 2.8GHz Intel Core 2 or System requirements higher, minimum 2GB RAM, USB 3.0 port, CD-ROM, 17” or larger display Software MAGUS View...
- Page 7 Mount type C-mount Illuminator body Metal Power supply DC 5V from the USB port of the computer Dimensions without package 200mm×436mm×400mm (WxHxD) Package dimensions (WxHxD) 271mm×431mm×630mm Weight without package Weight with package 11kg * Not included in the kit, available on request. ** The magnification of the microscope can be increased by using additional (optional) eyepieces and objectives.
- Page 8 Fig. 1. MAGUS Bio D250 Microscope. View from the right Eyepieces Coarse focusing tension 12. Trinocular tube adjusting knob Eyepiece tubes 13. Interpupillary distance Coarse focusing knob adjustment scale Revolving nosepiece Fine focusing knob 14. Camera Objectives 10. X/Y stage control knobs Specimen holder 11.
- Page 9 Fig. 2. MAGUS Bio D250 Microscope. View from the left Diopter adjustment ring Coarse focusing lock knob 12. Stand Analyzer slider insertion slot Coarse focusing knob 13. Microscope head Stage Fine focusing knob 14. Beam splitter lever Abbe condenser 10. Condenser focus knob Condenser mount 11.
- Page 10 MI C RO SC OP E PARTS STAN D The stand 12 (Fig.2) is a one-piece structure with the base. The base has Y-shaped stable ergonomic design. Parts attached to the microscope stand: – revolving nosepiece 3 (Fig. 1) with objectives 4 (Fig. 1) –...
- Page 11 MIC ROS COPE H E AD The microscope head is trinocular. The microscope head 13 (Fig. 2) provides the visual observation of the specimen image. The microscope head is installed in the mounting hole on the top of the microscope stand 12 (Fig. 2) and secured with a clamping screw 11 (Fig. 1).
- Page 12 Table 2 Numerical Working Coverslip, Objective identification System Magnification Color marking aperture distance, mm PL 4х/0.10 ∞/– 4х 0.10 19.8 PL 10х/0.25 ∞/– 10х 0.25 yellow PL 20х/0.4 ∞/0.17 20х 0.17 green PL 40х/0.65 ∞/0.17 40х 0.65 0.66 0.17 light blue PL 60х/0.80 ∞/ 0.17 60х...
- Page 13 CA M E R A Digital camera is intended for the brightfield and darkfield observations. It features low noise and high light sensitivity. The camera is mounted into the trinocular tube using the C-mount adapter from the microscope kit. The camera is powered through the USB port of a computer. U NPACK I NG AN D ASSEM BL IN G THE M ICROS COPE The assembly procedure is given in Fig.
- Page 14 Remove the microscope from the package. Check the scope of delivery using Section 7 of the User Manual. Inspect the microscope and its components for damage. Loosen the head locking screw 3, turn the microscope head 2 with the tubes toward the specimen stage and fix it with the head locking screw on the stand 1.
- Page 15 PL AC IN G THE SP EC IM E N Place the specimen 1 on the stage. Adjust the image by moving the stage control knobs 2 and 3 so that the observed section of the specimen is directly under the objective.
- Page 16 ADJ U STI N G THE E YE P I ECE T UB ES Use the diopter adjustment of the left eyepiece tube to compensate for the observer's ametropia. Start by setting the diopter adjustment to the zero. To do this, rotate the ring 1 to adjust the scale "0" to the indicator 2. While looking through the right eyepiece (with your left eye closed), bring the specimen into focus.
- Page 17 SE TTI N G U P KÖ H L ER IL LU MI NAT IO N In the light optical microscope, the image quality depends equally on the optics and on the illumination system, so adjusting the illumination is an important preparatory step. The illumination system affects the image resolution, comfort during long observation, and photo quality when using digital cameras.
- Page 18 When you switch to the objectives of other magnifications, do not change the height of the condenser, only adjust the opening of the field and aperture diaphragms. While adjusting the illumination, you should keep in mind that changing the size of the field diaphragm only affects the size of the illuminated field.
- Page 19 Dust cap screw Dust cap Trinocular tube centering screw Beam splitter lever Fig. 10. Using the camera To enable the camera: – Loosen the attachment screw 1. Remove the dust cap from the trinocular tube. – The microscope kit includes a C-mount adapter. Connect the camera to the adapter. –...
- Page 20 – Pull out the beam splitter lever 4 to the camera position. Look at the specimen on a monitor or display screen and make sure that the image of the target is in the center of the field of view. If the image deviates from the center of the field of view, adjust three centering screws 3 on the trinocular tube to move the target towards the center.
- Page 21 DARKF I EL D SL ID ER The darkfield slider is designed for the darkfield microscopy on objectives with apertures up to 0.9. The slider is a plate with two round openings. One opening is free for the brightfield technique. The second opening holds the darkfield diaphragm. The slider is inserted in the slot of the Abbe condenser 11 (Fig. 2).
- Page 22 Place the calibration slide on the stage instead of the specimen with the scaled side facing up. Using the scale of the calibration slide, calibrate the eyepiece scale for each objective that will be used for measurements. To do this, bring the image focus of the calibration slide scale into sharp focus in the plane of the eyepiece scale and rotate the eyepiece in the tube, setting the strokes of both scales in parallel.
- Page 23 T ROUB LES HOOTIN G Potential problems and remedies are given in Table 3. Table 3 Problem Cause Remedy EL ECTRI CAL C O M PO N E NTS The ON/OFF switch is off Switch on the ON/OFF switch The fuse has blown Replace the fuse No illumination in Switch off the power supply.
- Page 24 M ECH ANICAL COM P ONENTS The image does The coarse focusing tension adjusting not remain knob is loosened, causing the stage to Adjust the coarse focusing tension adjusting knob sharp during lower spontaneously observation The coarse The coarse tension adjusting knob is focusing knob is Loosen the tension of the coarse focusing knob overtightened...
- Page 25 SCO PE OF D EL IVE RY The assembly diagram is given in Fig. 15. The scope of delivery is listed in Table 4. Camera C-mount adapter Eyepice Eyepiece with a scale Revolving nosepiece Analyzer Objective Darkfield slider Polarizer Condenser Fig.
- Page 26 Table 4 Component Note D250T D250TL MICROSCOPE MAIN COMPONENTS Stand (with the transmitted light illuminator, power source and focusing mechanism built into the base) ICO Infinitive trinocular microscope head Revolving nosepiece Mounted on the stand Stage Mounted on the stand REPLACEABLE PARTS Centerable Abbe condenser A 0.9 darkfield condenser...
- Page 27 CA R E A N D M A INT ENA NC E RE PL ACING T HE B ULB A N D T HE FU SE Before replacing the bulb or fuse, turn the ON/OFF switch to "0" position (off). Unplug the power cord from the power outlet.
- Page 28 MAG U S WAR R A NT Y MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product.
- Page 29 ww w.magusmic ro.co m...
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