Magus LUM VD500L LCD User Manual

Fluorescence inverted digital microscope

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M AG U S LUM V D 5 0 0L LC D
F LUO RESC E NC E IN V ERT ED D I G I TA L M I C ROS C O P E
U S E R M A N U A L

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Summary of Contents for Magus LUM VD500L LCD

  • Page 1 M AG U S LUM V D 5 0 0L LC D F LUO RESC E NC E IN V ERT ED D I G I TA L M I C ROS C O P E U S E R M A N U A L...
  • Page 2 928 E 124th Ave. Ste D, Tampa, FL 33612, USA +1 813 468-3001 contact_us@levenhuk.com Levenhuk Optics s.r.o. (Europe) V Chotejně 700/7, 102 00 Prague 102, Czech Republic +420 737 004-919 sales-info@levenhuk.cz Magus® is a registered trademark of Levenhuk, Inc. © 2006–2024 Levenhuk, Inc. All rights reserved. www.levenhuk.com 20241111...
  • Page 3: Safety Precautions

    Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures.
  • Page 4 Camera 1. Never view the sun, another bright source of light or a laser through a camera - THIS IS DANGEROUS FOR YOUR EYESIGHT! 2. Do not disassemble the camera yourself. 3. Keep the camera away from moisture and do not use it in the rain. 4.
  • Page 5: Table Of Contents

    6 FLUORESCENCE OBSERVATIONS 7 USING OPTIONAL EQUIPMENT Using the eyepiece with a scale Using the calibration slide with a camera 8 TROUBLESHOOTING 9 SCOPE OF DELIVERY 10 CARE AND MAINTENANCE Replacing the bulb Replacing the fuse Maintenance 11 MAGUS WARRANTY...
  • Page 6: Description Of The Microscope

    MAGUS Lum VD500L LCD Fluorescence Inverted Digital Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation.
  • Page 7 PL L 10х/0.25/4.3; PL L 20x/0.40/8.0; PL L 40x/0.60/3.5 (spring-loaded); Objectives phase-contrast: PL L 10x/0.25/4.3 PHP2; PL L 20x/0.40/8.0 PHP2; magnification, x/aperture/ PL L 40x/0.60/3.5 PHP2 (spring-loaded); working distance, mm PL 4x/0.10* Fixed stage Stage size: 227mm×208mm Glass stage plate with a diameter of 118mm Mechanical XY stage attachment Moving range: 77mm×134.5mm Stage...
  • Page 8: Microscope Kit

    Software HDMI: built-in, USB: MAGUS View Mount type C-mount Illuminator body metal Power supply 12V/1A AC power adapter Monitor Type of matrix Screen diagonal, inch 13.3 Screen resolution, pix 3840x2160 (4K) Aspect ratio 16:9 Brightness, cd/m2 Number of displayed colors 16.7m...
  • Page 9 Fig. 1. MAGUS Lum VD500L LCD. View from the left 10. Fluorescence filter assembly 1. Eyecups 2. Eyepieces 11. Revolving nosepiece 3. Stand 12. Objectives 4. Transmitted/reflected light illuminator switch 13. Stage 5. ON/OFF switch 14. Condenser mount 6. Coarse focusing tension adjusting ring 15.
  • Page 10 Fig. 2. MAGUS Lum VD500L LCD. View from the right 1. Interpupillary distance adjustment ring 11. Object moving knob 2. Eyepiece tubes 12. Dish holder 3. Knob for switching the light path to the trinocular tube 13. Condenser moving knob 4.
  • Page 11: Microscope Parts

    2 MICROSCOPE PARTS STAN D The stand has stable ergonomic design. Parts attached to the microscope stand 3 (Fig. 1): – revolving nosepiece 11 (Fig. 1) with objectives – stage 13 (Fig. 1) – condenser mount 14 (Fig. 1) – illuminator 15 (Fig. 1) –...
  • Page 12: Microscope Head

    M ICROS COPE H EA D The trinocular head provides the visual observation of the specimen image. The microscope head is pre-installed on the stand 3 (Fig. 1). The eyepiece tube assembly is inserted into the head socket and secured with a screw. When installing the eyepiece tubes 2 (Fig.
  • Page 13: Transmitted Light Illuminator

    The 40x objectives have a spring-loaded mount to prevent mechanical damage to the front lens and the object. If objectives are damaged, we recommend repairing them in the service center. The objectives are intended to image the specimens through air. Do not use immersion oil. T RANS M ITTED LI GHT I LLUM INATO R The microscope illuminator allows for setting up Köhler illumination.
  • Page 14: Camera

    The camera is powered via a 12V/1A AC power adapter. M ON ITO R The monitor is designed to use a visualization system of the MAGUS microscope. It is connected to the camera mounted on the microscope to display the real-time images.
  • Page 15: Unpacking And Assembling The Microscope

    UNPACKING AND ASSEMBLING THE MICROSCOPE The assembly procedure is given in Fig. 3. Fig. 3. Assembling the microscope 1. Unpack the microscope and check the scope of delivery using Section 8 of the User Manual. 2. Take out the stand 1 and place it on a stable work table, remove protective packaging and dust cover. 3.
  • Page 16: Brightfield Observation Procedure

    8. Place the UV shield 9 and secure it with the screws. 9. Plug the power cord 10 of the reflected light illuminator into connector 11. 10. Plug the power cord into the connector on the stand and an AC outlet. 11.
  • Page 17: Using The Beam Splitter Lever

    US IN G T H E BEA M S P L I T T ER L EV ER Check the position of the beam splitter lever 1. Set it to the "eyepiece observation" position matching the symbol Symbol means that the light path is switched to the camera port. Fig.
  • Page 18: Placing The Specimen

    PL AC IN G THE SP EC IM E N Choose the appropriate dish holder from the kit based on its shape and size (Fig. 10). Install the dish holder on the stage. Fix the slide 1 or the dish with the studied sample in it. Fig.
  • Page 19: Setting Up Köhler Illumination

    When using high magnification objectives, raise the objective all the way up by rotating the coarse focusing knob and enable the coarse focusing lock knob. After that, focus on the specimen using the fine focusing knob. Adjust the coarse focusing tension. The tension of the coarse focusing knob is adjustable and is preset by the manufacturer for convenient use.
  • Page 20: Observing Specimens In Tall Glassware

    O BSE RVI N G SP EC IME N S IN TAL L GLAS SWA R E The microscope is equipped with a stage attachment 1 that enables movement of the specimen in longitudinal (Y) and lateral (X) directions. The object is moved by Y-axis 2 and X-axis 3 coaxial knobs, as shown in Fig.
  • Page 21: Calculating The Total Magnification

    CALCU LATI N G T HE TOTAL M AGN IF I CAT I ON The total magnification is the eyepiece power multiplied by the objective power. For example, if the eyepiece is 10х/22mm, and the objective is 40х/0.60, the total magnification of the microscope is 10 х...
  • Page 22: Using The Monitor

    – Place the 10х objective into the optical path. Looking through the eyepieces, bring the specimen into sharp focus. – Switch on the camera as described in the camera’s user manual. – Pull the beam splitter lever 3. If the image on the screen is blurry, adjust the focus using the fine focusing knob.
  • Page 23: Phase-Contrast Technique

    USB2.0 USB Mouse USB Video HDMI ON/OFF DC12V 1. Camera 4. Monitor 7. USB Type-С power connector 2. HDMI port 5. HDMI port 8. Audio output 3. HDMI cable 6. USB Type-С power connector Fig. 20. Using the monitor PHASE-CONTRAST TECHNIQUE The condenser allows utilizing the phase-contrast technique.
  • Page 24: Fluorescence Observations

    6 FLUORESCENCE OBSERVATIONS Transmitted light observations are performed as described above in the user manual. The following instructions apply only to the reflected light observations using the fluorescence technique. Select the appropriate fluorescence filters to match the dyes applied. The microscope kit includes a fluorescence filter assembly, which accommodates three sets of filters: DAPI, FITC, and TRITC.
  • Page 25: Using The Calibration Slide With A Camera

    Work out the value for one eyepiece division using each objective by formula Е = ТL/A, where: E – eyepiece division value Т – stage division value specified on the stage micrometer (0.01mm) L – number of stage micrometer divisions A –...
  • Page 26: Troubleshooting

    8 TROUBLESHOOTING Potential problems and remedies (Table 4): Problem Cause Remedy ELECTRI CAL COMP ONENTS The ON/OFF switch is off Switch on the ON/OFF switch The fuse has blown Replace the fuse No illumination in the field Poor electrical contact Check all the connectors.
  • Page 27: Scope Of Delivery

    MECHANI CAL C OMPONENTS The image does not remain The coarse focusing tension adjusting Adjust the coarse focusing tension adjusting sharp during observation knob is loosened, causing the stage knob to lower spontaneously The coarse focusing knob is too The coarse tension adjusting knob is Loosen the tension of the coarse focusing tight to rotate overtightened...
  • Page 28: Accessories And Spare Parts

    Infinity plan achromatic objective: PL L 10x/0.25 WD 4.3mm Infinity plan achromatic objective: PL L PHP2 20х/0.40 phase-contrast WD 8.0mm Infinity plan achromatic objective: PL L 20х/0.40 WD 8.0mm Infinity plan achromatic objective: PL L PHP2 40x/0.60 phase-contrast WD 3.5mm Infinity plan achromatic objective: PL L 40x/0.60 WD 3.5mm 10х/22mm eyepiece with eye relief 10x/22mm eyepiece with a scale...
  • Page 29: Care And Maintenance

    10 CARE AND MAINTENANCE R E PL AC IN G T HE BULB This microscope employs LED bulbs as a light source. The bulbs should be replaced by the equipment vendor or in a qualified service center. If you replace the LED yourself, the illumination function may be impaired.
  • Page 30: Magus Warranty

    8. Periodic inspection: the microscope should be regularly inspected and serviced to maintain its performance. MAGUS WARRANTY MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product.
  • Page 32 www.magusmicro.com...

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