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Summary of Contents for Ametek REICHERT 4SPR
- Page 1 ® REICHERT 4SPR 4 Channel SPR System User’s Guide...
- Page 2 Corporation. Clamp and Scrubber are copyrighted by Thomas Morton and David Myszka. Tracedrawer is a trademark of Ridgeview Instruments AB. AMETEK is a registered trademark of AMETEK, Inc. All other trademarks are property of their respective owners. The information contained in this document was accurate at time of publication. Specifications subject to change without notice.
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Page 3: Table Of Contents
Table of Contents Warnings and Cautions .................. 4 Symbol Information ..................5 Introduction ....................6 Features & Functions ..................7 Setup ......................8 Unpacking & Installation ................8 Fluidics Setup – Pump Module ............... 11 Setup Autosampler ................. 13 Installation of Sensor Chip and Flow Cell ..........14 Fluidics Setup - Valves and Flow Cell ............. -
Page 4: Warnings And Cautions
Warnings & Cautions ® Reichert Technologies (Reichert) is not responsible for the safety and reliability of this instrument when assembly, disassembly, repair, or modification is made by unauthorized dealers or persons. Or, when the instrument is not used in accordance with this User’s Guide. WARNING: DO NOT REMOVE OR DEFEAT THE EARTH GROUND CONNECTION ON THE INPUT CONNECTOR TO EACH COMPONENT OR THE UNIT’S POWER CORD. -
Page 5: Symbol Information
Symbol Information Symbol Information The following symbols appear on the instrument: Warning symbol indicating risk of electrical shock (all three modules) Warning symbol indicating a heated surface (SPR Module). CAUTION: Replace with same type and rating fuse 5x20mm, 6.3A, 250V AC Glass Fast Acting (SPR Module); 5x20mm, 1.6A, 250V Quick Acting Glass (Pump Module) Warning symbol indicating important operating and maintenance instructions that are included in this User’s Guide... -
Page 6: Introduction
Please retain this manual for future reference and to share with other users. Additional copies can be ® obtained from your authorized Reichert dealer or from the Reichert’s Customer Service Department Tel: +1 716-686-4500 Fax: +1 716-686-4555 Email: reichertspr.lifesciences@ametek.com 13309000-102 REV B... -
Page 7: Features & Functions
Features and Functions The Reichert4SPR SPR system has three separate modules connected by easily addressable tubing. The modules are the Pump, the Autosampler and the SPR Instrument, respectively. The SPR Instrument Module features four channels with single (channels 1, 2 or 3), dual (channels 1 & 2 or 3 &... -
Page 8: Setup
Setup Unpacking 1) Remove the system from packaging and check contents. If an item is missing, contact the factory for resolution. 2) Remove the User's Guide and read it carefully prior to operation of the system. Parts Identification • Pump Module (P/N 13309100) •... - Page 9 Back of SPR Instrument Autosampler Module plugs Module in here via RS232 COM cable MOXA USB switch plugs in here via a RS232 COM On/Off Switch cable Power cord USB Port to computer Back of Pump Module Degasser On/Off Switch RS232 Com cable from On/Off Switch MOXA serial hub plugs in...
- Page 10 Back of RS232 COM cable from SPR Module plugs in here Autosampler Module On/Off Switch Power cord 8) A fluidics kit containing all the necessary fittings and tubing should be opened and its contents checked against a packing list. 9) Two sensor chips are also included for initial use with your instrument. 13309000-102 REV B...
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Page 11: Fluidics Setup - Pump Module
Fluidics Setup – Pump Module Installation of the Syringe into the Pump 1) Loosen the set screw on the pump and drop the plunger base into the opening, and tighten the set screw securely (fingertight). 2) Pull the syringe up into the pump valve and tighten (luer connection). Adapters Step 2 - Screw top of syringe into pump valve... - Page 12 Pump Module Inlet to Autosampler from Fingertight fittings Degasser – use orange 0.02” ID PEEK tubing Power light for Pump Module – Use beige 0.04” ID PEEK illuminates when turned on, Tubing to connect from blinks when pumping syringe inlet to degasser Use beige 0.04”...
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Page 13: Setup Autosampler
Setup - Autosampler 1) The Port Layout for the tubing connections for the Reichert Autosampler is shown in the schematic below. The needle wash tubing and the tubing to the needle (Ports 3 and 4) are already plumbed for use. 2) Several sample loops are supplied with the instrument and can be exchanged depending on what volume is best for your experiment (sample loop is connected at ports 2 and 5). -
Page 14: Installation Of Sensor Chip And Flow Cell
Installation of Sensor Chip and Flow Cell Overview: The SPR sensor chip is optically coupled to the prism surface using refractive index matching oil. The flow cell mounts on the top of the sensor chip and is locked into place via a clamp mechanism. - Page 15 side. If it appears to have shiny gold edges, that is the glass (bottom) of the chip (acts as a mirror) and should be set onto the prism surface. Top of Slide Bottom of Slide 4) Slowly lower the sensor chip and place it onto the prism surface near the back of the prism and on top of the oil droplet.
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Page 16: Fluidics Setup - Valves And Flow Cell
Flow cell Fluidics Setup - Valves and Flow Cell © 1) As shown in the figure above, external tubing is attached to the valves via PTFE (Teflon ) or © PEEK tubing. 2) For the inlet tubing at each port, use 6 inches of red (0.005" ID) tubing and for the outlet ©... - Page 17 Port 4 to Outlet Channel 1 on flow cell Port 3 to Inlet Channel 3 on flow cell Port 4 not used Valves Port 3 to Outlet Channel 3 on flow cell Inlet from Autosampler Outlet to Waste Port 1 to Inlet Channel 1 on flow cell Port 2 to Outlet...
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Page 18: Programs
Programs Installation See the Quick Start Guide “Reichert4SPR 4Ch Version 2_0_1 Quick Start Guide.docx” for information on how to configure and install the MOXA hardware and drivers. For details on installing the data acquisition and analysis software, see the Reichert4SPR software manual. - Page 19 Anti-Bovine Serum Albumin (Anti-BSA) is injected over the surface at a range of concentrations and the data is analyzed to determine the binding kinetics and affinity of this antibody-antigen interaction. CHEMICALS: EDC (Sigma Aldrich Cat No. 03449-5G) NHS (Pierce Cat No. 24500) 10 mM Sodium acetate pH 5.2 (need pH about 1 unit below pI of ligand to be coupled) 1 M ethanolamine pH 8.5 (Sigma >98% Cat No.
- Page 20 6) Prepare a 1 mg/mL solution of BSA in water, dilute 20 L of this BSA stock solution to 1 mL with 10 mM sodium acetate pH 5.2 into a 2 mL HPLC vial, cap and place in the position indicated in Sample Tray 1 (2A).
- Page 21 13) Make a 1 mg/mL stock of Anti-BSA in running buffer. Dilute 288 L of the 1 mg/mL Anti-BSA stock with 1512 L of PBST in a 2 mL HPLC vial to prepare the highest concentration solution of Anti-BSA, 32 nM. 14) Serially dilute in half (pipet 900 L of buffer in four separate 2 mL HPLC vials and add 900 L of Anti-BSA from each higher concentration in prior vial) to make an additional four concentrations, 16, 8, 4 and 2 nM.
- Page 22 21) At the end of the experiment, you can go directly to Data Analysis or Export to either Tracedrawer or Scrubber for later analysis. Refer to the Reichert4SPR software manual for further details on how to use Tracedrawer for analysis or to the Scrubber manual for how to use Scrubber software.
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Page 23: Cleaning & Maintenance
Cleaning & Maintenance Cleaning The Reichert4SPR is a precision optical instrument and should be cleaned regularly to maintain optimal performance. External Cleaning The outer cases of all the instrument components can be cleaned with a mild soap solution or ethanol whenever needed. Prism Cleaning The prism surface can be cleaned with a Kimwipe or with ethanol if all the oil does not wipe off. -
Page 24: Troubleshooting
Troubleshooting The following chart presents some possible errors and the steps that you can take to correct the issue. If a problem persists, please contact Reichert (see Introduction section of this manual for contact information). Chart of Common Errors Issue Probable Cause Possible Solution I am seeing a flat (-1) -
Page 25: Specifications
Specifications Catalog Number 13309000 Physical Dimensions SPR Instrument Module Size Weight, unpacked: 20 lbs (9 kg) Height: 14.2” (36 cm) Weight, packed: 22 lbs (10 kg) Width: 6.75” (17.15 cm) Depth: 22.6“ (57.5 cm) Autosampler Size Weight, unpacked: 46.2 lbs (21 kg) Height: 14.2”... -
Page 26: Device Classification
Transportation & Storage: Temperature and Relative Humidity: Not Specified Keep Dry Device Classification Electrical Protection: Class I Operating Mode: Continuous Disposal This product does not generate any environmentally hazardous residues. At the end of its product life, follow your local laws and ordinances regarding the proper disposal of this equipment, or contact Reichert. -
Page 27: Catalog Information
CATALOG INFORMATION Reichert4SPR System Catalog Number Description 13309000 Reichert4SPR System 13309000 Reichert4SPR SPR Instrument Module 13309100 Reichert4SPR Pump Module 13309200 Reichert4SPR Autosampler Accessories • 13309100-403 Pump Valve • 13309100-404 Pump Syringe • 13309000-333 Flow Cell • 13207120-003 Autosampler Needle Optional Accessories •... -
Page 28: Warranty
Warranty This product is warranted by Reichert Technologies against defective material and workmanship under normal use for a period of one year from the date of invoice to the original purchaser. (An authorized dealer shall not be considered an original purchaser.) Under this warranty, Reichert’s sole obligation is to repair or replace the defective part or product at Reichert’s discretion. - Page 29 2. Call either Reichert directly (for direct purchases) or the dealer you purchased the product from and report the shortage. The materials are packed at the factory and none should be missing if the box has never been opened. 3. Claims must be filed within 30 days of purchase. CLAIMS FOR DAMAGES IN TRANSIT Our shipping responsibility ceases with the safe delivery in good condition to the transportation company.
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Page 30: Reichert Contact Information
Manufacturer Reichert, Inc. 3362 Walden Avenue, Suite 100 Depew, NY 14043 Toll Free: 888-849-8955 Phone: 716-686-4500 email: reichertspr.lifesciences@ametek.com www.reichertspr.com Authorized European Representative AMETEK GmbH Reichert Business Unit Carl-von-Linde-Strasse 42 85716 Unterschleissheim / Munich Germany Tel: +49 (89) 315 8911 0...
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