YSI 2700D User Manual

Biochemistry analyzer

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Summary of Contents for YSI 2700D

Page 1 (217) 352-9330 | Click HERE Find the YSI 2700D at our website:...
Page 2 Biochemistry Analyzer Who’s Minding the Planet? find out at Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
Page 3 Description Standard Features General Specifications Chemistry Performance Specifications How to Use this Manual Unpacking and Notes on Safety Major Component Identification Reagent Preparation Enzyme Membrane Installation Power Up Procedures Fluid System Priming Printer Paper Installation 2-11 Instrument Parameter Programming 2-12 Initial Setup Example: Step-by-Step...
Page 4 Introduction Service Selections Setup Selections Diagnostic Selections 5-17 Sensor Technology Fluid System Measurement Methodology Baseline Stability Calibration Linearity Temperature Compensation Level Sensing and Sipper Interference Software Structure Daily Maintenance Calibration Pumping System Maintenance Sample Chamber Cleaning Membrane Replacement Probe Cleaning Tubing Replacement...
Page 5 '( ' ) * '( + ,% '( '( - , . '( / . ,0 1 '( . 2 % '( 3 4 5 % 2% '( 6 %7 - % - '( ...
Page 6 The Test Tube Holder Pivoted Out Figure 3.4 The Manual Station Figure 5.1 2700 SELECT Software Structure Figure 5.2 YSI 2700 SELECT Menu Flow Chart Figure 5.3 2700 SELECT Major Components 5-17 Figure 6.1 Sensor Probe and Enzyme Membrane Figure 6.2 2700 SELECT Fluid System Figure 6.3...
Page 7 The YSI Model 2700 SELECT Biochemistry Analyzer is a laboratory instrument intended for use in research, food-processing and bioprocessing applications. THE MODEL 2700 SELECT IS NOT FOR HUMAN MEDICAL DIAGNOSTIC USE OR FOR HUMAN PERFORMANCE EVALUATION. The Model 2700 SELECT offers precision and specificity comparable to more time consuming and rigorous methods, but with greater speed, convenience and sensitivity.
Page 8 YSI provides a full line of reagents to support the most common chemistries. YSI offers the Model 2710 Turntable for batch sampling of up to 24 tubes or vials. The 2710 Turntable is controlled by the 2700 SELECT software via a control cable (provided). The results of batch sampling are displayed and printed by the 2700 SELECT.
Page 9 • Single and dual chemistry configurations. • Minimal sample preparation. • Sample aspiration from a variety of tubes. • Selectable sample aspiration volume (5 to 65 µL). • Sample results in about one minute. • Fluid sensing minimizes carry-over. •...
Page 10 Sample size: Adjustable from 5 to 65 microliters (aspirated volume) Response Time: Sample results in 60 seconds, Complete sample-to-sample cycle in 90 seconds (May vary with analyte and sample matrix.) Power requirement: 110-120 VAC or 220-240 VAC 50-60 Hz 50 Watts nominal Working environment: Ambient temperature:...
Page 11 ! "#$ " Dextrose (D-Glucose) YSI Membrane: 2365 0-9 g/L at 25 µL sample size, Detection Range: 0-25 g/L at 10 µL sample size Calibration Point: 2.50 g/L Linearity Check Point: 9.0 g/L Precision (CV,n=10): ±2% Linearity (0 to Cal Point): ±5%...
Page 12 Lactose YSI Membrane: 2702 Detection Range: 0-25 g/L Calibration Point: 5.00 g/L Linearity Check Point: 25.0 g/L Precision (CV,n=10): ±2% Linearity (0 to Cal Point): ±5% Linearity (Cal to Range Max): Typical Membrane Working Life: 10 days Membrane O-ring Color:...
Page 13 Potential Substrate Interference: Ethanol NOTE: For the following chemistries YSI believes that you will be able to measure these analytes for many applications in the ranges specified below. However, YSI makes no claims with respect to precision or linearity. For each analyte below you will need to prepare the calibrator and linearity standards for your application.
Page 14 &' !( If your instrument is configured for single channel chemistry, this instruction manual contains more information than you require, but the measurement technology, and the principles and techniques of operation remain the same. This manual is organized in such a way as to give you the quickest possible start in operating the instrument.
Page 15 Rating: F1-F5, 1A; F6-F7, 2A. These fuses are NOT operator replaceable. 8.2 See Appendix G for mains plug wiring and fusing instructions. If the equipment is used in a manner not specified by YSI, the protection provided by the equipment may be impaired.
Page 16 Referring to Figure 2.1 and 2.2, read through the following descriptions and familiarize yourself with the major components. NOTE: In maintenance kits, service manuals and part lists the "Sipper" may be referred to as the Sipper Needle or Sipper Tube. Figure 2.1 Inside Front View of the 2700 SELECT Artisan Technology Group - Quality Instrumentation ...
Page 17 The Remote Communication Port is an RS-232 serial port. It is used to interface with host computers or other laboratory instruments. The Auxiliary Port is an interface connector for use with accessories like the YSI 2710 Turntable. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
Page 18 Figure 2.2 Back View of the 2700 SELECT The Line Voltage Selector selects either 110-120 or 220-240 volt operation. It houses the fuses and pulls out of the case for fuse replacement. The Power Receptacle is a power inlet. One end of the power cord plugs into this receptacle, while the other end plugs into an electrical outlet.
Page 19 YSI 2357 is recommended for use with all YSI Enzyme Membranes except YSI 2702 Galactose Oxidase membranes (use YSI 2705), YSI 2786 Ethanol Membranes (use YSI 2787) and YSI 2725 Methanol Membranes (use YSI 1579). All YSI buffers may be ordered separately.
Page 20 Figure 2.4 Sample Chamber/Sensor Configurations One or more packs of YSI Immobilized Enzyme Membranes is provided in the starter supplies. Each pack contains four membranes. Enzyme Membrane O-rings are color-coded for each type of chemistry. For dual channel configurations it is important that you note which probe, black or white, you use to install specific membranes.
Page 21 (YSI 2392 or equivalent). Place one drop of salt solution on the probe face. Using the plastic membrane holder, press the O-ring membrane assembly gently onto the probe face (Figure 2.5).
Page 22 If the voltage selection is incorrect, review Section 7.9 for correct voltage selection and fuse requirements or contact YSI Customer Service or your Dealer Representative. If the voltage selection is correct, plug in the unit using the power cord packaged with the instrument.
Page 23 ( Since it may take an hour or more to initially stabilize the probes when setting up for the first time, now is a good time to prime the fluid system. You have already reconstituted the appropriate buffer and transferred it to the Buffer Bottle. You have also installed the appropriate calibrator solution and installed the appropriate Enzyme Membrane(s) in the Sample Chamber.
Page 24 Press [1] for Lower sipper for fine alignment. The Sipper will move closer to the small opening in the stainless steel cone. Fine align sipper then select 0-Exit 1-Test sipper position The tip of the Sipper should be exactly centered above this opening. If necessary, loosen the adjustment screw again and position the Sipper exactly.
Page 25 Press [2] for Buffer. The Sipper will enter the Sample Chamber and the Buffer Pump will begin to prime the fluid system with buffer solution. The fluid system is completely primed when buffer flows from the steel cone at the top of the sample chamber. Press [2] again, if necessary. Select service: 1-Sipper 2-Buffer 3-Cal 4-Stir speed 5-Monitor 6-Turntable Press [3] for Cal.
Page 26 The 2700 SELECT setup is menu driven. Once set up, the system parameters are maintained in memory. In the event of power loss, the 2700 SELECT has a battery backup to maintain its memory. Follow the procedure below to program your instrument parameters. IMPORTANT: You move through the 2700 SELECT menus by selecting options on the display.
Page 27 NOTE: YSI has assigned default values for each of these parameters, but provides the flexibility for the user to change values to optimize a particular application. DURING INITIAL SETUP, YSI RECOMMENDS THAT YOU USE THE DEFAULT SETTINGS FOR THE CHEMISTRIES YOU CHOOSE.
Page 28 Press [6] for Default. The display will read: Reset all system parameters? No 1-No 2-Yes 6-Default. In this menu you have the option to reset all default parameters, i.e., those set in the software to serve your needs for all standard applications. Using this command also requires that you reset date, time, and all other general parameters.
Page 29 Let us assume you have installed a YSI 2329 Lactate Membrane on the black probe and a YSI 2365 Glucose Membrane on the white probe. YSI 2357 buffer has been reconstituted and poured into the Buffer Bottle and YSI 2776 Standard (2.50 g/L dextrose and 0.50 g/L L-lactate) has been installed into the Calibrator Bottle position.
Page 30 Press [1] for General. General setup: 1-Date/Time 2-Contrast 3-RS232 4-Radix 5-Serial# 6-LevelSensor Press [1] for Date/Time. Use the number keys to change entries. Press [ENTER] to confirm each entry. Enter date and time as required Year> 98 Enter year. Enter date and time as required Month>...
Page 31 It will be printed in the detailed report format and will be very useful if technical assistance or repair is required. Since YSI uses alpha-characters in the serial #, we recommend that you use a space for these characters. Example: 98B12345AB can be entered as 98 12345.
Page 32 Enter Cal Station # > 1 Press [ENTER] to confirm that the calibrator solution (YSI 2776) is installed at Station #1 (Cal Well of the Sample Chamber). The display now returns to the Measurement parameter setup menu level.
Page 33 > 05.62 The value you observe (5.62 mmol/L) is equivalent to 0.50 g/L, the concentration stated on the YSI 2776 calibrator label. NOTE: If you are using the default values and you choose a concentration unit other than g/L, the value automatically changes. That is, 0.50 g/L becomes 5.62 mmol/L or 500 mg/L. When non-standard calibration values are used, however, the appropriate numbers must be entered whenever the measurement unit is changed.
Page 34 Press [ENTER] to confirm Dextrose. Select WHITE unit of measurement: g/L 1-mmol/L 2-mg/L (ppm) 3-g/L 4-% (w/v) Press the appropriate number to change the default setting, if necessary. When the desired unit of measurement is selected, press [ENTER] to confirm and move to the next parameter.
Page 35 SamplingProtocol setup: 1-SipperHeight 2-Replicates 3-ID 4-Station# 5-Turntable Press [2] for Replicates. Prompt replicate cycle? No 1-No 2-Yes Since our example setup requests triplicate analysis of the sample, we must activate replicates. Press [2] for Yes. Prompt replicate cycle? Yes 1-No 2-Yes Press [ENTER] to confirm and return to Sampling protocol setup.
Page 36 Press [2] for AutoStandby. Enter autostandby time in hour 0 to disable > 2 This entry defines the number of hours the unit will continue to update calibration and be ready to sample. The default setting is "2". Note that you would press [0], then [ENTER] to disable autostandby which maintains the unit in a "sample ready"...
Page 37 Press [4] for Report. Select sample report format: Brief 1-None 2-Brief 3-Detail The default setting is "brief" report. Press [ENTER] to choose brief Sample report, then press [ENTER] again to choose brief Cal report. See Appendix D for example printouts of each type of report.
Page 38 Select instrument function 1-Service 2-Setup 3-Diagnostic Now press [0] again to return to Main menu. Please select instrument mode [RUN] [STANDBY] [MENU] Now refer to Section 4 to learn about the recommended system parameters for your particular chemistry application. Next use this information to setup the 2700 SELECT as we have demonstrated above.
Page 39 8 $ 9 . You were previously instructed to prime the fluid system. Since then, you have been learning about the menu selections. Now it is time to check the probe baseline current to determine if the installed enzyme membrane sensor(s) and probe(s) have equilibrated and are stable enough to initiate calibration.
Page 40 Once the installed membranes and probes are equilibrated, the probe baseline current typically runs below 2 nA. This equilibration may take a day or two and does not usually affect operation during the first day since autocalibrations compensate for probe current drift. When enzyme membrane replacements are required in the future, stabilization will occur much more rapidly, usually within several minutes of installation, if there has been no power disruption to the instrument.
Page 41 When you power on or reset your 2700 SELECT the Main Menu appears on the instrument display. Please select instrument mode [RUN] [STANDBY] [MENU] From Main Menu you may enter any of three modes by pressing the appropriate function key. The modes are RUN, STANDBY and MENU.
Page 42 IMPORTANT: The RUN and STANDBY keys act as toggle keys: You enter RUN Mode by pressing [RUN] and you exit RUN Mode by pressing [RUN]. You enter STANDBY Mode by pressing [STANDBY] and you exit STANDBY Mode by pressing [STANDBY]. In each action above you must confirm your intention by pressing [2] or [1] for yes or no, respectively, then press [ENTER] to confirm your choice.
Page 43 For the purposes of demonstrating basic operation, choose the high level standard for the chemistry you are set up to run. For example, if you have installed a YSI 2365 (glucose oxidase) membrane, calibrate with YSI 2776 standard and use YSI 1531 standard (containing 9.00 g/L dextrose) to run as a sample.
Page 44 Figure 3.2 Sampling Stations Figure 3.3 The Test Tube Holder Pivoted Out Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
Page 45 For the purposes of learning basic operation, compare the instrument value with the labeled value of the YSI standard. The 'linearity' standard should be within ±5% of the label value. For example, if you calibrated the 2700 SELECT at 2.50 g/L dextrose using YSI 2776 as the calibrator solution, YSI 1531 (9.00 g/L) should read between 8.55 g/L and 9.45 g/L dextrose.
Page 46 The results are then displayed and printed as shown above. Again compare the value reported by the instrument with the value on the label of the YSI 1531 Standard (9.00 g/L dextrose). Repeat calibration and sampling if the result is not within specified tolerance limits of ±5% as described above.
Page 47 NOTE: This test is recommended for all YSI Enzyme Membranes except Galactose Oxidase Membranes (YSI 2702). In these membranes FCN is a component of the supply buffer. High baseline error messages may provide similar information regarding the structural integrity of the installed membrane.
Page 48: Linearity Test
* If you use units other than g/L for the FCN test, refer to Appendix A for conversion values. Linearity Test Use the appropriate YSI linearity standard to test the linear range of the chemistry or chemistries you have chosen. See the list below to be certain you are testing with the correct standard solution.
Page 49 If you are setting up to measure galactose or hydrogen peroxide using one of the YSI Enzyme Membrane Sensors, see Section 4 for more information. * If you use units other than g/L for the linearity test, refer to Appendix A for conversion values.
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Page 51 This produces a probe signal current. In many procedures utilizing enzymes, the enzyme is discarded with the sample after the analysis is made. YSI's technology allows preservation of the enzyme by immobilizing it in a patented membrane structure.
Page 52 YSI Application Notes. If you would like to discuss your application or inquire about other uses for the 2700 SELECT, contact YSI Technical Support (address and phone information on the back cover of this manual).
Page 53 In order to configure your instrument to measure a particular chemistry analyte, you need to: » Approximate the analyte concentration or range of concentrations to be measured. » Decide if you must dilute your sample, and, if required, determine an appropriate dilution factor and diluent.
Page 54 Calibration Method (2-CalMethod). In most chemistry setups, you will select “One Station”, and the calibration station # will be “Station #1” (Calibration Well). However, you have options to configure this in other ways. » Select One Station, but assign Cal Station #2. Station #2 is the Test Tube Holder Station.
Page 55 30 sec Note: See Appendix A if concentration unit conversion is required. * For applications requiring linearity performance to 25.0 g/L, YSI 2777 (25.0 g/L dextrose) may be used as a linearity standard provided the sample size is 10 µL.
Page 56 " ## This is a direct reading of L-Lactate (L-Lactic Acid) in solution at the enzyme sensor. The enzyme L-Lactate Oxidase is immobilized in the YSI L-Lactate Membrane. L-Lactate + O + Pyruvate L-Lac Oxidase System Buffer YSI 2357 Calibrator Std YSI 2776 (0.50 g/L L-Lactate)
Page 57 This is a direct reading of sucrose in solution at the enzyme sensor. Three enzymes are co- immobilized in the YSI Sucrose Membrane: Invertase, Mutarotase, and Glucose Oxidase. α-D-Glucose + (Fructose) Sucrose + H Invertase α-D-Glucose β-D-Glucose Mutarotase β-D-Glucose + O + D-Glucono-δ-Lactone...
Page 58 They may interfere by producing artificially high lactose readings. » The enzyme membrane integrity test involving the use of YSI 2363 Potassium Ferrocyanide solution is not informative since ferrocyanide exists in the 2705 system buffer.
Page 59 Oxidase is immobilized in the enzyme membrane. Ethanol + O + Acetaldehyde Alcohol Oxidase System Buffer YSI 2787 Calibrator Std YSI 2390 (Solution A, 2.00 g/L) Linearity Std YSI 2390 (Solution B, 3.20 g/L) Black Probe YSI 2786 Alcohol Oxidase Membrane White Probe n/a * 10 µL (for best linearity)
Page 60 End Point 30 sec * For applications requiring linearity performance to 25.0 g/L, YSI 2777 (25.0 g/L dextrose) may be used as a linearity standard provided the sample size is 10 µL. ** If you have a dual channel unit, either Black or White, or both Black and White probes can be configured for this chemistry.
Page 61 For example, to express results as a choline hydroxide value, program the calibrator value as 204 mg/L when using the YSI 2772 Choline Standard calibrator solution. Your results will now reflect the concentration of choline hydroxide in the sample.
Page 62 They may interfere by producing artificially high galactose readings. » The enzyme membrane integrity test involving the use of YSI 2363 Potassium Ferrocyanide solution is not informative since, ferrocyanide exists in the 2705 system buffer.
Page 63 This membrane contains immobilized nonenzymatic protein to produce diffusion properties similar to those exhibited by YSI Enzyme Membranes. The electrochemical reaction, which is common to all YSI Enzyme Sensors, is the following: + 2e Platinum anode...
Page 64 To express results as an MSG value, change the glutamate units to a w/v expression, and enter one of the following: 936 mg/L, 0.936 g/L, or 0.094 %. Use YSI 2755 Calibrator Standard (5.00 mmol/L Glutamate) to calibrate the instrument. Sample results will then reflect MSG concentration.
Page 65 # This is a direct reading of glutamine in solution at the enzyme sensor. Two enzymes are co- immobilized in the YSI Glutamine Membrane: Glutaminase and Glutamate Oxidase. L-Glutamine L-Glutamate + NH Glutaminase + α-Ketoglutarate + NH L-Glutamate + O...
Page 66 Oxidase is immobilized in the enzyme membrane. Methanol + O + Formaldehyde Alcohol Oxidase System Buffer YSI 1579 Calibrator Std YSI 2726 (Solution A, 1.00 g/L) Linearity Std YSI 2726 (Solution B, 2.50 g/L) Black Probe YSI 2725 Methanol Membrane White Probe n/a * 15 µL (for best linearity)
Page 67 System Buffer YSI 2357 Calibrator Std YSI 2776 (2.50 g/L dex; 0.50 g/L L-lact) Linearity Std YSI 1531 (9.00 g/L dex) and YSI 1530 (2.67 g/L L-lact) Black Probe YSI 2329 L-Lactate Membrane White Probe YSI 2365 Dextrose Membrane 25 µL...
Page 68 The constant 1.9 corrects for the weight of fructose that is not sensed at either probe, and the water of hydrolysis. System Buffer YSI 2357 Calibrator Std YSI 2776 and YSI 2780 Linearity Std YSI 1531 and YSI 2778 Black Membrane YSI 2703...
Page 69 , and appropriate dilutions of the sample to first measure free dextrose, then treat one sample with invertase and analyze for dextrose liberated from the sucrose. Contact YSI Customer Service, or your dealer representative for an application note explaining this approach in detail.
Page 70 Total Glutamate is the combined glutamine and glutamate probe signal. Free Glutamate is the glutamate not derived from Glutamine reaction. System Buffer YSI 2357 Calibrator Std YSI 2755 and YSI 2736 Linearity Std YSI 2756 and YSI 2737 Black Membrane YSI 2735...
Page 71 In this section you will learn about the specific commands required to move through the 2700 SELECT menu structure. You should begin by studying Figure 5.1 below, recognizing that the instrument has two operating modes and a menu mode. Also refer to Figure 5.2, next page. The operating modes are RUN Mode and STANDBY Mode.
Page 72 [RUN] [STANDBY] [MENU] Figure 5.2 YSI 2700 SELECT Menu Flow Chart REMEMBER: You move through the 2700 SELECT menus by selecting options on the display. You can press [MENU] to get back to the Main Menu, and [0] to back up to the previous display.
Page 73 Service functions are primarily used during initial setup and anytime reagent changes are made. Both Cal Pump and Buffer Pump can be primed using this menu level. In addition, Sipper alignment and Stir Bar speed adjustment can be performed from this level. Use the display messages and text below to learn the service functions.
Page 74 # $ " % " & & " '$$ " "! & If you press [5] for Monitor, you will access the functions used to align and prime the YSI 2730 Monitor and Control Accessory. Refer to the YSI 2730 Monitor and Control Accessory User’s Manual for details.
Page 75 $ Setup menu is where you will enter system parameters that define the specific information required to run the 2700 SELECT for your particular application. You will set general information such as date, time, display and print formats. You will enter specific information related to chemistry selection, calibration values, concentration units, calibration and sampling protocols, communication parameters, and other features.
Page 76 Enter date and time as required Month> 2 Enter date and time as required Date> 14 Enter date and time as required Hour> 17 Enter date and time as required Minute> 22 Select and/or confirm the format you desire for printed dates; (month/day/year) or (day/month/year).
Page 77 Select number of data bits: Seven bit 1- Seven bit 2-Eight bit Select parity: Odd 1-None 2-Even 3-Odd 4-Low 5-High Select number of stop bits: One bit 1-One bit 2-Two bits Select handshaking type: RTS/CTS 1-RTS/CTS 2-XON/XOFF 3-None If you choose XON/XOFF, you will go one menu level deeper to enter or confirm XON and XOFF values.
Page 78 Enter this number in memory. It will be printed for certain report formats. NOTE: YSI uses alpha-characters in the serial number. We recommend that you press [SPACE] for the first alpha-character and omit the last two. Example, 98B01234AB can be entered as 98 01234.
Page 79 The default location is Station #1 (Cal Well). The choices are #1 and/or #2. Number 2 is the Test Tube Holder location. With Accessories such as the YSI 2710 Turntable and YSI 2730 Monitor and Control Accessory, stations #4 and #5 are also options.
Page 80 Press [1] for Next chemistry to scroll through the choices in the menu. The choices are L- Lactate, Sucrose, Lactose, Galactose, Peroxide, Ethanol, Choline, L-Glutamate, L-Glutamine, Methanol, none, and Dextrose. The Black probe default chemistry is L-Lactate. Press [ENTER] to confirm and continue to the unit of measurement parameter. The choices are grams/liter (g/L), millimoles/liter (mmol/L), milligram/liter (mg/L), which is often referred to as parts per million (ppm), and percent (weight/volume).
Page 81 Enter WHITE end point in seconds > 30 Press [ENTER] to confirm and return to Measurement parameter setup. Measurement parameter setup 1-SampleSize 2-CalMethod 3-Black 4-White You have now completed MeasParameter setup. Press [0] to return to the Select setup level. Select setup: 1-General 2-MeasParameter 3-RunMode 4-Report 5-PrntSetup 6-Default Press [3] for RunMode.
Page 82 Test Tube Holder (#2) and sample from the Cal Well (#1). If you have the YSI 2710 Turntable or YSI 2730 Monitor and Control Accessory, stations #4 and #5 are also available. See the appropriate manual for assignments.
Page 83 3-Sample 4-Cal shift 5-Sample error You may alter any of these parameters to suit your application, however, you may compromise precision and/or accuracy when doing so. YSI's stated specifications are based on the default settings shown above. -./0-' ! $ " # ! ! 1& ...
Page 84 Autocal setup: 1-Temperature 2-Time 3-Sample 4-Cal shift 5-Sample error Press [2] for Time. Enter autocal time interval in minute > 15 Confirm value shown or enter and confirm any number of minutes from 0 to 30000. Autocal setup: 1-Temperature 2-Time 3-Sample 4-Cal shift 5-Sample error Press [3] for Sample.
Page 85 Press [0] to return to RUN mode setup. RUN mode setup: 1-SampleProtocol 2-AutoStandby 3-AutoCal 4-Monitor You have now completed Run mode setup. Press [0] to return to Select setup menu level. Select setup: 1-General 2-MeasParameter 3-RunMode 4-Report 5-PrntSetup 6-Default Press [4] for Report. The display will read: Select sample report format: Brief 1-None 2-Brief 3-Detail Press [ENTER] to confirm sample report format and move to cal report format.
Page 86 Default will clear some or all of your selections and restore Default system parameters. You will likely use Default to return to YSI-recommended settings if you have been experimenting with the selections and would like to start at the beginning.
Page 87 ' Diagnostic menu functions are used primarily during troubleshooting. In this menu, you will learn to gain individual control of components in the instrument. For example, you can turn motors on and off, and even "exercise" motor-controlled devices. You can run pumps to check fluid movement.
Page 88 As discussed in the Introduction to this section, you must exit RUN Mode or STANDBY Mode to gain complete access to the diagnostic menu functions. To exit Run or Standby modes, press the appropriate key ([RUN] or [STANDBY]), then follow the prompts to exit. You will now be in Main Menu mode and the display will appear as shown below.
Page 89 Press [6] to initiate repeated motions in the same plane. We refer to this as" Exercise". The motion (horizontal or vertical) is determined by the last command you chose. The instrument counts the number of cycles and displays them as shown below. When you press a key to terminate the exercise mode, the printer records the activity and number of cycles completed.
Page 90 The display will now update to give two new selections as shown below. Sipper pump diagnostic 1-Home 2-Aspirate separator 3-Exercise Press [2] for Aspirate separator. A short retraction of the plunger should occur. This simulates the aspiration of an air bubble to isolate sample from the buffer stream. The display updates to read the following.
Page 91 Press [0] to return to the five diagnostic selections. NOTE: Unless the 2730 Monitor and Control pump is installed, 4-ExtPump will give no response, Refer to the YSI 2730 Monitor and Control Accessory User’s Manual for details of external pump operation.
Page 92 B:LAC 11.77 nA W:DEX 23.17 nA 1-Flush 2-Calibrator 3-Sample Next, press [1] for Flush to clear the calibrator solutions from the Sample Chamber. The flush will take about 30-40 seconds. If the baseline has not returned to levels you previously observed, you may want to initiate a second flush cycle.
Page 93 Hit keys - Hold [CANCEL] to exit Key> [RUN] released To exit the keypad test, press and hold down the [CANCEL] key. Hit keys - Hold [CANCEL] to exit Key> [CANCEL] held down The following message appears after you exit the keypad test. I/O diagnostic 1-Keypad 2-Display 3-Printer Press [2] for Display.
Page 94 Press [0] to return to the five diagnostic selections. Select diagnostic 1-Motor 2-Pump 3-Probe 4-I/O 5-Sensor Press [5] for Sensor. You may select one of three categories of sensors: temperature, fluid level or detection, and optosensors for a variety of motion status signals. Sensor diagnostic 1-Temperature 2-LevelSensor 3-OptoSensor Press [1] for Temperature.
Page 95 If the Sipper Pump plunger is at "home", i.e. extended, the SipperPump message should be ON; if the plunger is in any position other than home, the message should be OFF. If there is a test tube in the Test Tube Holder and it is in a vertical orientation, the "TestTube" message should be ON;...
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Page 97 The sensor technology of the YSI 2700 SELECT is based on the principles conceived by Dr. Leland Clark of Children’s Hospital Foundation, Cincinnati, Ohio. The immobilized enzyme membrane was invented by YSI and is covered by U.S. Patent 4,073,713. This sensor technology has been used successfully since 1975 in the YSI 23A Blood Glucose Analyzer;...
Page 98 The face of the probe, covered by the membrane, is situated in a buffer-filled sample chamber into which a sample is injected. Some of the substrate diffuses through the membrane. When it contacts the immobilized oxidase enzyme, it is rapidly oxidized, producing hydrogen peroxide. See Reaction 1, using glucose as an example.
Page 99 The 2700 SELECT fluid and electromechanics system is schematically described below in Figure 6.2. Figure 6.2 2700 SELECT Fluid System Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
Page 100 (i ). Typical net responses for the 2700 SELECT are between 10 and 25 nA (nanoamps) for YSI calibration solutions. The maximum net probe current is about 500 nanoamps. ! The 2700 SELECT monitors the probe baseline current for nA activity and stability. If an unstable baseline is detected, the buffer pump will continue to flush the sample chamber with buffer.
Page 101 " # ! To maintain a sample ready status, the 2700 SELECT self-calibrates. Calibrating establishes the sensors’ response, in nanoamps of current, to a known concentration of substrate. The sensors calibration response must be above 5 nA. A response below this value will result in an error.
Page 102 This occurs as an enzyme membrane ages. It is necessary to periodically check sensor linearity. YSI offers linearity standards for all of the recommended calibration values. See Section 1.4 for details. If you chose to identify your own calibration value and measurement range, keep this failure mode in mind.
Page 103 * The 2700 SELECT has two operation modes: RUN Mode, where calibration and sample analysis occur and STANDBY Mode, where system power is on, but only a few maintenance functions occur. This conserves reagents during extended periods of inactivity. The 2700 SELECT also has menu selections for service, setup and diagnostics. The Main Menu of the software therefore provides three options: RUN, STANDBY, and MENU.
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Page 105: Preventive Maintenance
[RUN] [STANDBY] [MENU] Preventive Maintenance The YSI 2788 Preventive Maintenance Kit contains all supplies necessary to keep your 2700 SELECT operating properly. Perform the Calibration Pumping System Maintenance detailed in Section 7.2 at least once every month. Perform the maintenance procedures in Sections 7.3 to 7.11 every 6 months or as necessary.
Page 106: Check For Leaks
Please select instrument mode [RUN] [STANDBY] [MENU] Select instrument function 1-Service 2-Setup 3-Diagnostic Select service: 1-Sipper 2-Buffer 3-Cal 4-Stir speed 5-Monitor 6-Turntable If the calibrator bottle cap assembly needs cleaning, make sure it is thoroughly rinsed and dried before it is attached to a fresh bottle of calibrator solution. If you are calibrating from a test tube at the Test Tube Holder Station (#2), evaporation is a major concern.
Page 107: Clean Up Spills
Clean up Spills Spills should be cleaned up promptly to prevent biohazard build-up and corrosion. Clean any spills of biological material from the sample area. If there is any evidence of salt build-up around the base of the sample chamber, follow the procedure in Section 7.3 to disassemble the chamber and clean the base.
Page 108 It is necessary to periodically clean both the sample chamber and the base plate it sets on. Move the Sipper to the manual sample position. From the Main Menu press [MENU]. Next press [3] for Diagnostic, then press [1] for Motor, then press [1] for Sipper motor. Press [1] for Up, to raise the Sipper from the Sample Chamber, then press [4] for Clockwise to move the Sipper over to the Manual Sample position.
Page 109 Clean up any salt deposits or fluid on the base plate. Be sure that the base plate and all other parts are dry. Refer to Figure 7.1 and reassemble the chamber. Remember to return the stir bar to the chamber and be sure to install the chamber seal O-ring. Insert the probes into the chamber, installing new membranes (see Section 2.4) or O-rings as necessary.
Page 110: Sensor Maintenance
With normal use, enzyme sensors may become fouled and cease to operate in a normal mode. A fouled sensor’s output current will decrease and calibration may become unstable. Since the severity of fouling will vary, listed below are methods of cleaning which should be matched to the cleaning needs.
Page 111 & The buffer and calibrator pumps in the 2700 SELECT are peristaltic tubing pumps. The tubing life depends on instrument usage. The pump tubes should be replaced at least every 6 months, more frequently if necessary. Other tubes become loose at the fittings and should also be replaced every 6 months.
Page 112 Figure 7.3 Pump Tubing Installation Make sure that the correct length of tubing is sticking out of the pump (1 to 1½″ [2.5 to 3.5 cm] of the large diameter tubing on the left side of the buffer pump), then rotate the roller assembly while pushing the tubing down into the pump ahead of the roller.
Page 113 Slot Rear Housing Roller Assembly Tubing Front Housing Tube Bushing Figure 7.4 Pump Assembly When all tubes are properly connected, see Section 2.6 to readjust the Sipper and prime the calibrator and buffer systems. The Sipper can be damaged if it is not properly aligned or if its alignment is disturbed. If your Sipper needs replaced, remove the screw that mounts it to the sipper arm, disconnect the tubing and remove the Sipper.
Page 114 . It may be necessary to replace the fuses in the 2700 SELECT. New fuses may be purchased from YSI or obtained from many local electrical component suppliers. Be sure to obtain the correct fuse rating as indicated below. UNPLUG THE INSTRUMENT FROM THE MAINS SUPPLY.
Page 115 / Replace the Sipper Pump seals every 6 months. Heavy usage may warrant more frequent replacement. Disconnect the tubings to the Sipper Pump. Remove the Sipper Pump head from the instrument wall (see Figure 7.5). Figure 7.6 Sipper Pump Head Removal Pull the white pump base from the clear pump housing.
Page 116 Figure 7.7 Sipper Pump Seal Replacement Reassemble the pump, position the plunger as shown in figure 7.7 and install it back on the instrument. Figure 7.8 Sipper Pump Plunger Position !"#"$% 0) 12&3) 4″' '5.6&( () ))7 7-12 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
Page 117 Apply Lubriplate 105 grease (supplied in YSI 2788 Preventive Maintenance Kit) to two or three threads of the Acme screw. Normal operation will distribute the grease along the length of the screw.
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Page 119 » Use the troubleshooting chart in this section to assist you in identifying the problem, then use the chart to guide you to a corrective action. If the problem cannot be resolved, contact YSI Customer Service (address and phone information in Appendix F). When communicating with service personnel, please indicate the serial number of the instrument.
Page 120 FB current 0.76 nA Sample size 25 uL Base shift -1.95 % Fri 02/22/98 08:12:34 Slope 0.09 nA/min YSI 2700D - 98 01234 End Point 30 Sec ----------------------- Cal shift -0.10 % Temperature 26.33 °C Sample size 25 uL Thu 02/22/98...
Page 121 IB current (Initial Baseline Current). The initial baseline current is monitored before a sample or calibration and while stabilizing baseline current upon entering RUN Mode. To successfully enter RUN Mode, the IB current must be stable and below 6 nA. PL current (Plateau Current).
Page 122 SYMPTOM: ERROR: Baseline POSSIBLE CAUSE: Pinched, leaking or disconnected tube. ACTION: Fix or replace tubing. SECTION: POSSIBLE CAUSE: Sipper misaligned. ACTION: Check Sipper alignment. SECTION: POSSIBLE CAUSE: Buffer pump not operating properly. ACTION: Replace tubing. SECTION: POSSIBLE CAUSE: Stir bar not in chamber. ACTION: Disassemble chamber and reinstall stir bar.
Page 123 SYMPTOM: ERROR: Empty Buffer Bottle (Continued) POSSIBLE CAUSE: Level sensor cable not plugged in. ACTION: Plug in cable. SECTION: SYMPTOM: ERROR: Empty Calibrator Bottle POSSIBLE CAUSE: Low calibrator level. ACTION: Install new calibrator. SECTION: POSSIBLE CAUSE: Level sensor cable not plugged in. ACTION: Plug in cable.
Page 124 SYMPTOM: ERROR: Sample Chamber Temperature Out of Range POSSIBLE CAUSE: Ambient temperature too cold or hot. ACTION: Operate at ambient temperatures between 15 and 35 degrees C. SECTION: SYMPTOM: ERROR: Sipper Interference or Fault in Fluid Level Detector POSSIBLE CAUSE: Sipper detects a conductive body.
Page 125 POSSIBLE CAUSE: Printing on wrong side of paper. ACTION: Install paper correctly. SECTION: POSSIBLE CAUSE: Using wrong type of paper. ACTION: Install correct thermal paper (YSI 2751). SECTION: SYMPTOM: Fail FCN Test POSSIBLE CAUSE: Damaged or old membrane. ACTION: Replace membrane.
Page 126 SYMPTOM: Fail Linearity Test POSSIBLE CAUSE: Probe assignment incorrect. ACTION: Make correct assignment. SECTION: POSSIBLE CAUSE: Damaged or old membrane. ACTION: Replace membrane. SECTION: POSSIBLE CAUSE: Contaminated or old calibration or linearity standard. ACTION: Repeat test with new standards. SECTION: POSSIBLE CAUSE: Assigned concentration range beyond practical limits.
Page 127 This section describes the communications protocol between the 2700 SELECT and a host computer. The 2700 SELECT has an RS-232 serial port. When you install the appropriate hardware interface (e.g., shielded NULL modem cable), the 2700 SELECT communicates in ASCII over this interface.
Page 128 Hardware Handshaking Signals. The four handshaking signals used to control the data flow are RTS/CTS and DTR/DCD. The 2700 drives RTS when it wishes to transmit and waits for CTS before transmitting each character. Likewise, the 2700 asserts DTR when it is ready to receive data and receives only when DCD is asserted.
Page 129 You can select the 2700 handshaking protocol, RTS/CTS or XON/XOFF. In addition, you can select baud rate, data length (bits per character), parity, stop bit, and configuration (multipoint or point-to-point). For information on communications setup, see Section 4.2, Menu Setup. The communications menu is a submenu of General setup.
Page 130: Error Codes
Error Codes Code 1 a) 2700 is not in remote control mode. This condition generally indicates that you invoked the TN1 command (enter Run Mode remotely) when the 2700 was not in remote mode. You must first successfully invoke TR1 (turn on remote control mode).
Page 131 The 2700 can be placed in one of two communications modes: Result Reporting Mode or Remote Control Mode. The selection between modes is made by the remote host. The default mode at power-up is Result Reporting Mode. 2700 Data Base. The 2700 keeps a data base of 32 sample results and 1 calibration result in its battery-backed RAM (random access memory).
Page 132 Data Transmission Records. The data report format from "2700 to HOST" is transmitted as shown in the example below. Information includes: » time » date » temperature (sample chamber) » node address (re: multidrop mode; instrument identification) » ID (sample identification number) »...
Page 133 123456789012345678901234567890123456789012345678901234567890123456789012345 13:22:34 02/13/98 23.56 123 123456789 H2O2 12345.78 mmol/L 0000\ <cr><lf> 13:22:34 02/13/98 23.56 123 123456789 H2O2 12345.78 mmol/L 0000 <cr><lf> 13:22:34 02/13/98 23.56 123456789 H2O2 12345.78 mmol/L 0000\ <cr><lf> 13:22:34 02/13/98 23.56 123456789 H2O2 12345.78 mmol/L 0000 <cr><lf> 15:12:04 02/13/98 23.56 123 -1 H2O2 45.78 nA 0000\ <cr><lf>...
Page 134 The fields of the data transmission records are more specifically defined below in Figure 9.5. A ruler line is used simply to help you identify the offset and field widths in the report. We have used the following symbols to depict changes from early 2700 software vs versions 2.41 and higher.
Page 135 Command Description. For quick reference, the communications commands are divided into five functional groups: process, report database, database management, control and report system information. For details on process commands, see "Remote Control Commands", later in this section. PROCESS Process calibration Process sample from calibration well (Station #1) Process sample from test tube holder (Station #2) Process turntable batch (Station #4)
Page 136 Common Commands. The commands below can be invoked in either Remote Control Mode or in Result Reporting Mode, unless otherwise stated. Available commands are those in the Report Database and Report System Information categories above. When Report Status (RY) is invoked, the command obtains the 2700 status. The status includes communications mode, sample result status, calibration result status, machine status and remote command status.
Page 137 When Report Last Transmission (RX) is invoked, the last transmission of the 2700 is sent to the host. It is used when the response received by the 2700 contains an unknown character, is not in the correct format, or whenever in doubt due to communications error. Host to 2700: Report last transmission 2700 to Host:...
Page 138 Remote Control Commands. The commands outlined above in the "Process" category can be invoked only in Remote Control Mode. If the system is not in the Remote Control Mode, you must first invoke the Turn Remote Control On (TR1) command. Note: For each command from Host to 2700, the 2700 sends back an acknowledgment (A) or an error code.
Page 139 Host to 2700: Exit Run Mode and switch to Standby Mode or Menu Mode. 2700 to Host: Acknowledge. 2700 exits Run Mode. 2700 is not in Remote Control Mode. 2700 is busy in Run Mode. When you invoke TN1 (Enter Run Mode), the instrument enters Run Mode from Standby or from “Menu Mode”.
Page 140 When you invoke the Process Sample (PS) command, the Host commands the 2700 to sample from one of four locations depending on the specific command (PS1, PS2, PS4 or PS5). If the 2700 is running an autocalibration, calibration stabilization or baseline stabilization, the command is acknowledged and the sample is processed as soon as the instrument finishes the existing task.
Page 141 When you invoke the RM (Report Parameters) command the instrument reports to you the current system time, date and measurement parameters. For ease of deciphering this information, the report format is almost identical to that of the sample and calibration reports. The only difference is that the Error Field, beginning at column 62, contains additional information about the calibration parameters.
Page 142 When you invoke the RI (Report Information) command, the instrument uploads the instrument setup that is not reported by the RM command, except communications and local control parameters. The information reported by this command is coded to conserve space. You must know the codes listed in Figure 9.7 to understand these setup parameters.
Page 143 Additional remote control commands related to changing the external monitoring parameters and aborting a turntable run are described below. Monitor Parameter Modification Commands (MP, MT, MR, MO, MS). You may use the commands described below to change monitor setup parameters remotely. change external pump purge time to t, where t is in seconds change monitor time interval to t, where t is in minutes change precal time interval to t, where t is in minutes...
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Page 145 In the YSI 2700 SELECT menu selection procedure you have the option to assign units of concentration. You will find this in the Setup Menu under measurement parameters, black and white probes. There are default values set based on calibration solutions offered by YSI. Below is a table of unit conversions for these calibration solutions and other concentrations of interest.
Page 146 Example Conversions Beginning with 2.50 g/L dextrose, convert this to mg\L, then % and finally to mmol/L: 1. Multiply by unit conversion(s) 2. Cancel units common in “numerator” and “denominator” 3. Multiply numbers ? mg/L = 2.50 g/L = (2.50 g/L)(1000 mg/g) = (2.50)(1000) mg/L = 2500 mg/L ? % (w/v) = 2.50 g/L = (2.50 g/L)(0.1 L/dL) = (2.50)(0.1) g/dL = 0.250 g/dL = 0.250 % (Note: g/dL is g/100ml or percent)
Page 147 Linearity Test. Concentration Unit Conversion mg/L Chemistry (ppm) (w/v) mmol/L (g/mole) 0.47 0.047 4.54 Choline 0.45 0.045 4.32 (104) 0.43 0.043 4.10 9.45 9,450 0.945 52.5 Dextrose 9.00 9,000 0.900 50.0 (180) 8.55 8,550 0.855 47.5 3.36 3,360 0.34 73.1 Ethanol 3.20 3,200...
Page 148 0.01 0.29 (342) NOTE: Use the values from the preceding tables only when calibrating with the appropriate YSI calibrator solution: Choline (2772), Dextrose (2776), Ethanol (2790), L-Glutamate (2755), L- Glutamine (2736), L-Lactate (2776), Methanol (2726) and Sucrose (2780). See Section 3, Basic Operation, Daily Operational Checks for cross references.
Page 149 Enzyme Membrane Kits YSI # Product Description Quantity 2329 L-Lactate Membrane Kit 4/kit 2365 Dextrose Membrane Kit 4/kit 2786 Ethanol Membrane Kit 4/kit 2701 Blank Membrane Kit (hydrogen peroxide) 4/kit 2702 Galactose Oxidase Membrane Kit 4/kit 2703 Sucrose Membrane Kit...
Page 150 Printer Paper (5 rolls/box) 2788 Preventive Maintenance Kit Contact YSI Incorporated or your authorized dealer representative for more information on ordering supplies and reagents for the YSI 2700 SELECT. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
Page 151 You can learn about the system parameters by referring to Section 5.3, Setup, in this manual. If you have a YSI 2710 Turntable, refer to Section 3 in the 2710 Turntable Operation Manual. Printed Setup Information The printouts for single channel and dual channel 2700’s are shown on the following page.
Page 152 Level sensor : ON ------------------------ Cal Report : Brief SampleReport : Brief DateFormat : MM/DD/YY Software revision: 2.56 YSI 2700D - 98 01234 Thu 02/26/98 09:22:36 ------------------------ Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
Page 153 When you setup your YSI 2700 SELECT from the Setup Menu, you have the option to print the calibration and sample results in "brief" or "detail" format. You also have the option not to print calibration values. Review Section 5.3, Setup, specifically choice 4-Report in the Setup selection menu.
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Page 155 The Federal Communications Commission defines this product as a computing device and requires the following notice: This equipment generates and uses radio frequency energy and if not installed and used properly, may cause interference to radio and television reception. It has been type tested and found to comply with the limits for a Class A or Class B computing device in accordance with the specification in Subpart J of Part 15 of FCC Rules, which are designed to provide reasonable protection against such interference in a residential installation.
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Page 157 The YSI Model 2700 Analyzer is warranted for one year from date of purchase by the end user against defects in materials and workmanship, exclusive of batteries. Within the warranty period, YSI will repair or replace, at its sole discretion, free of charge, any product that YSI determines to be covered by this warranty.
Page 158: Cleaning Instructions
Disposition costs will be the responsibility of the sender. When service is required, either at the user's facility or at YSI, the following steps must be taken to insure the safety of our service personnel.
Page 159: Packing Instructions
Packing Instructions 1. Clean and decontaminate items to insure the safety of the handler. 2. Complete and include the Cleaning Certificate. 3. Place the product in a plastic bag to keep out dirt and packing material. 4. Use a large carton, preferably the original, and surround the product completely with packing material.
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Page 161 Figure G.1 Line Power Cord and Plug Wiring Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
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Page 163 Proper precautionary lab practices should be followed when handling biological hazards. Suggested cleaning and disinfecting solutions include: » Isopropanol 70% » Sodium hypochlorite, 5000 ppm free available chlorine (1:10 solution of household bleach) Decontamination Procedures Remove and discard all tubing. New tubing is provided in preventive maintenance kit. Empty waste bottle and wash with disinfecting agent.
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Page 165 1. Users should not open the front access door during operation, internal components may be susceptible to ESD (electrostatic discharge). 2. Users are cautioned not to touch the sipper needle during operation, as discussed in the users manual. The sipper mechanism is equipped with contact-sensing circuitry for the purposes of fluid detection and human safety.
Page 166 1725 Brannum Lane Yellow Springs, Ohio 45387 USA 062521 937 767-7241 • 800 659-8895 • Fax 937 767-8058 A23781L Info@ysi.com • www.YSI.com July 2000 © 2000 YSI Incorporated Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

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