I have fully PMed and changed everything on the YSI 2700 select but it is not flushing buffer or calibrator fluid through the sample chamber. It doesnt even sound like the motor is triggering. How Can I fix this

1 comments:

Mr. Anderson

May 22, 2025

To fix the issue of the YSI 2700 SELECT not flushing buffer or calibrator fluid through the sample chamber:

1. Try "massaging" the tubing to relieve any blockage.
2. If that does not work, disconnect the tubing and use a syringe to force air or fluid through until you find and clear the blockage.

These steps help restore fluid flow through the system.

This answer is automatically generated

Summary of Contents for YSI 2700 SELECT

Page 1 Biochemistry Analyzer Who’s Minding the Planet? find out at...
Page 2 Description Standard Features General Specifications Chemistry Performance Specifications How to Use this Manual Unpacking and Notes on Safety Major Component Identification Reagent Preparation Enzyme Membrane Installation Power Up Procedures Fluid System Priming Printer Paper Installation 2-11 Instrument Parameter Programming 2-12 Initial Setup Example: Step-by-Step...
Page 3 Introduction Service Selections Setup Selections Diagnostic Selections 5-17 Sensor Technology Fluid System Measurement Methodology Baseline Stability Calibration Linearity Temperature Compensation Level Sensing and Sipper Interference Software Structure Daily Maintenance Calibration Pumping System Maintenance Sample Chamber Cleaning Membrane Replacement Probe Cleaning Tubing Replacement...
Page 4 '( ' ) * '( + ,% '( '( - , . '( / . ,0 1 '( . 2 % '( 3 4 5 % 2% '( 6 %7 - % - '( ...
Page 5 Figure 2.1 Inside Front View of the 2700 SELECT Figure 2.2 Back View of the 2700 SELECT Figure 2.3 Bottles and Level Sensor Cables Figure 2.4 Sample Chamber/Sensors Figure 2.5 Enzyme Membrane Installation Figure 2.6 Line Voltage Selector Figure 2.7...
Page 6 The Model 2700 SELECT can be set up to measure a number of different analytes, but switching between them may entail some time and effort. Configured as a single channel unit, the Model 2700 SELECT provides quick measurements of any one of the following analytes: •...
Page 7 YSI provides a full line of reagents to support the most common chemistries. YSI offers the Model 2710 Turntable for batch sampling of up to 24 tubes or vials. The 2710 Turntable is controlled by the 2700 SELECT software via a control cable (provided). The results of batch sampling are displayed and printed by the 2700 SELECT.
Page 8 • Single and dual chemistry configurations. • Minimal sample preparation. • Sample aspiration from a variety of tubes. • Selectable sample aspiration volume (5 to 65 µL). • Sample results in about one minute. • Fluid sensing minimizes carry-over. •...
Page 9 Sample size: Adjustable from 5 to 65 microliters (aspirated volume) Response Time: Sample results in 60 seconds, Complete sample-to-sample cycle in 90 seconds (May vary with analyte and sample matrix.) Power requirement: 110-120 VAC or 220-240 VAC 50-60 Hz 50 Watts nominal Working environment: Ambient temperature:...
Page 10 ! "#$ " Dextrose (D-Glucose) YSI Membrane: 2365 0-9 g/L at 25 µL sample size, Detection Range: 0-25 g/L at 10 µL sample size Calibration Point: 2.50 g/L Linearity Check Point: 9.0 g/L Precision (CV,n=10): ±2% Linearity (0 to Cal Point): ±5%...
Page 11 Lactose YSI Membrane: 2702 Detection Range: 0-25 g/L Calibration Point: 5.00 g/L Linearity Check Point: 25.0 g/L Precision (CV,n=10): ±2% Linearity (0 to Cal Point): ±5% Linearity (Cal to Range Max): Typical Membrane Working Life: 10 days Membrane O-ring Color:...
Page 12 Potential Substrate Interference: Ethanol NOTE: For the following chemistries YSI believes that you will be able to measure these analytes for many applications in the ranges specified below. However, YSI makes no claims with respect to precision or linearity. For each analyte below you will need to prepare the calibrator and linearity standards for your application.
Page 13 &' !( If your instrument is configured for single channel chemistry, this instruction manual contains more information than you require, but the measurement technology, and the principles and techniques of operation remain the same. This manual is organized in such a way as to give you the quickest possible start in operating the instrument.
Page 14 In the event of damaged or missing parts, contact YSI Customer Service or your Dealer Representative immediately. Note that reagents for the 2700 SELECT Analyzer are not packaged in the same carton as the instrument. These materials must be ordered separately as starter supplies and will arrive in a separate package.
Page 15 Referring to Figure 2.1 and 2.2, read through the following descriptions and familiarize yourself with the major components. NOTE: In maintenance kits, service manuals and part lists the "Sipper" may be referred to as the Sipper Needle or Sipper Tube. Figure 2.1 Inside Front View of the 2700 SELECT...
Page 16 The Reset Switch is located on the back of the instrument. It is used to hard reset the operating system. The Remote Communication Port is an RS-232 serial port. It is used to interface with host computers or other laboratory instruments. The Auxiliary Port is an interface connector for use with accessories like the YSI 2710 Turntable.
Page 17 Figure 2.2 Back View of the 2700 SELECT The Line Voltage Selector selects either 110-120 or 220-240 volt operation. It houses the fuses and pulls out of the case for fuse replacement. The Power Receptacle is a power inlet. One end of the power cord plugs into this receptacle, while the other end plugs into an electrical outlet.
Page 18 YSI 2357 is recommended for use with all YSI Enzyme Membranes except YSI 2702 Galactose Oxidase membranes (use YSI 2705), YSI 2786 Ethanol Membranes (use YSI 2787) and YSI 2725 Methanol Membranes (use YSI 1579). All YSI buffers may be ordered separately.
Page 19 "black" or the "white" side of the sample chamber.) The 2700 SELECT is shipped to you already configured as a Single Channel or a Dual Channel unit. For the single channel configuration, the white probe is a blank (see Figure 2.4).
Page 20 (YSI 2392 or equivalent). Place one drop of salt solution on the probe face. Using the plastic membrane holder, press the O-ring membrane assembly gently onto the probe face (Figure 2.5).
Page 21 If the voltage selection is incorrect, review Section 7.9 for correct voltage selection and fuse requirements or contact YSI Customer Service or your Dealer Representative. If the voltage selection is correct, plug in the unit using the power cord packaged with the instrument.
Page 22 ( Since it may take an hour or more to initially stabilize the probes when setting up for the first time, now is a good time to prime the fluid system. You have already reconstituted the appropriate buffer and transferred it to the Buffer Bottle. You have also installed the appropriate calibrator solution and installed the appropriate Enzyme Membrane(s) in the Sample Chamber.
Page 23 Press [1] for Lower sipper for fine alignment. The Sipper will move closer to the small opening in the stainless steel cone. Fine align sipper then select 0-Exit 1-Test sipper position The tip of the Sipper should be exactly centered above this opening. If necessary, loosen the adjustment screw again and position the Sipper exactly.
Page 24 Press [2] for Buffer. The Sipper will enter the Sample Chamber and the Buffer Pump will begin to prime the fluid system with buffer solution. The fluid system is completely primed when buffer flows from the steel cone at the top of the sample chamber. Press [2] again, if necessary. Select service: 1-Sipper 2-Buffer 3-Cal 4-Stir speed 5-Monitor 6-Turntable Press [3] for Cal.
Page 25 The 2700 SELECT setup is menu driven. Once set up, the system parameters are maintained in memory. In the event of power loss, the 2700 SELECT has a battery backup to maintain its memory. Follow the procedure below to program your instrument parameters.
Page 26 NOTE: YSI has assigned default values for each of these parameters, but provides the flexibility for the user to change values to optimize a particular application. DURING INITIAL SETUP, YSI RECOMMENDS THAT YOU USE THE DEFAULT SETTINGS FOR THE CHEMISTRIES YOU CHOOSE.
Page 27 Press [6] for Default. The display will read: Reset all system parameters? No 1-No 2-Yes 6-Default. In this menu you have the option to reset all default parameters, i.e., those set in the software to serve your needs for all standard applications. Using this command also requires that you reset date, time, and all other general parameters.
Page 28 This is a straight-forward setup utilizing one calibration solution to perform simultaneous chemistries. Follow along by pressing keys on your 2700 SELECT. This exercise will be a good learning tool. After completing the exercise, you will be directed to parts of the manual related to your specific chemistry interests.
Page 29 Press [1] for General. General setup: 1-Date/Time 2-Contrast 3-RS232 4-Radix 5-Serial# 6-LevelSensor Press [1] for Date/Time. Use the number keys to change entries. Press [ENTER] to confirm each entry. Enter date and time as required Year> 98 Enter year. Enter date and time as required Month>...
Page 30 It will be printed in the detailed report format and will be very useful if technical assistance or repair is required. Since YSI uses alpha-characters in the serial #, we recommend that you use a space for these characters. Example: 98B12345AB can be entered as 98 12345.
Page 31 Enter Cal Station # > 1 Press [ENTER] to confirm that the calibrator solution (YSI 2776) is installed at Station #1 (Cal Well of the Sample Chamber). The display now returns to the Measurement parameter setup menu level.
Page 32 > 05.62 The value you observe (5.62 mmol/L) is equivalent to 0.50 g/L, the concentration stated on the YSI 2776 calibrator label. NOTE: If you are using the default values and you choose a concentration unit other than g/L, the value automatically changes. That is, 0.50 g/L becomes 5.62 mmol/L or 500 mg/L. When non-standard calibration values are used, however, the appropriate numbers must be entered whenever the measurement unit is changed.
Page 33 Press [ENTER] to confirm Dextrose. Select WHITE unit of measurement: g/L 1-mmol/L 2-mg/L (ppm) 3-g/L 4-% (w/v) Press the appropriate number to change the default setting, if necessary. When the desired unit of measurement is selected, press [ENTER] to confirm and move to the next parameter.
Page 34 SamplingProtocol setup: 1-SipperHeight 2-Replicates 3-ID 4-Station# 5-Turntable Press [2] for Replicates. Prompt replicate cycle? No 1-No 2-Yes Since our example setup requests triplicate analysis of the sample, we must activate replicates. Press [2] for Yes. Prompt replicate cycle? Yes 1-No 2-Yes Press [ENTER] to confirm and return to Sampling protocol setup.
Page 35 Press [2] for AutoStandby. Enter autostandby time in hour 0 to disable > 2 This entry defines the number of hours the unit will continue to update calibration and be ready to sample. The default setting is "2". Note that you would press [0], then [ENTER] to disable autostandby which maintains the unit in a "sample ready"...
Page 36 YOU HAVE NOW COMPLETED THE INITIAL SETUP EXAMPLE. As you learn more about the 2700 SELECT you will gain greater familiarity with the menu system. If you would like to revisit levels of the Select setup menu, press the appropriate number(s).
Page 37 [RUN] [STANDBY] [MENU] Now refer to Section 4 to learn about the recommended system parameters for your particular chemistry application. Next use this information to setup the 2700 SELECT as we have demonstrated above. HINT: Use the Print Setup option to confirm that you have correctly setup your instrument.
Page 38 Once the baseline currents are below 6 nA and reasonably stable, press [MENU] to return to Main Menu. You may need to allow an hour or more to establish stability when initially setting up the 2700 SELECT. Please select instrument mode...
Page 39 Once the installed membranes and probes are equilibrated, the probe baseline current typically runs below 2 nA. This equilibration may take a day or two and does not usually affect operation during the first day since autocalibrations compensate for probe current drift. When enzyme membrane replacements are required in the future, stabilization will occur much more rapidly, usually within several minutes of installation, if there has been no power disruption to the instrument.
Page 40 When you power on or reset your 2700 SELECT the Main Menu appears on the instrument display. Please select instrument mode [RUN] [STANDBY] [MENU] From Main Menu you may enter any of three modes by pressing the appropriate function key.
Page 41 IMPORTANT: The RUN and STANDBY keys act as toggle keys: You enter RUN Mode by pressing [RUN] and you exit RUN Mode by pressing [RUN]. You enter STANDBY Mode by pressing [STANDBY] and you exit STANDBY Mode by pressing [STANDBY]. In each action above you must confirm your intention by pressing [2] or [1] for yes or no, respectively, then press [ENTER] to confirm your choice.
Page 42 For the purposes of demonstrating basic operation, choose the high level standard for the chemistry you are set up to run. For example, if you have installed a YSI 2365 (glucose oxidase) membrane, calibrate with YSI 2776 standard and use YSI 1531 standard (containing 9.00 g/L dextrose) to run as a sample.
Page 43 Figure 3.2 Sampling Stations Figure 3.3 The Test Tube Holder Pivoted Out...
Page 44 For the purposes of learning basic operation, compare the instrument value with the labeled value of the YSI standard. The 'linearity' standard should be within ±5% of the label value. For example, if you calibrated the 2700 SELECT at 2.50 g/L dextrose using YSI 2776 as the calibrator solution, YSI 1531 (9.00 g/L) should read between 8.55 g/L and 9.45 g/L dextrose.
Page 45 The results are then displayed and printed as shown above. Again compare the value reported by the instrument with the value on the label of the YSI 1531 Standard (9.00 g/L dextrose). Repeat calibration and sampling if the result is not within specified tolerance limits of ±5% as described above.
Page 46 Enter autostandby time in hour 0 to disable> 2 If you would like your 2700 SELECT never to leave Run Mode, press [0] and it will continually self-calibrate and maintain a "sample ready" status. Otherwise, enter the number of hours (0 to 65,535) you would like to maintain a "sample ready" status before reverting to STANDBY Mode.
Page 47: Linearity Test
* If you use units other than g/L for the FCN test, refer to Appendix A for conversion values. Linearity Test Use the appropriate YSI linearity standard to test the linear range of the chemistry or chemistries you have chosen. See the list below to be certain you are testing with the correct standard solution.
Page 48 If you are setting up to measure galactose or hydrogen peroxide using one of the YSI Enzyme Membrane Sensors, see Section 4 for more information. * If you use units other than g/L for the linearity test, refer to Appendix A for conversion values.
Page 49 3-10...
Page 50 The YSI 2700 SELECT has been designed to provide considerable flexibility with respect to how it may be configured. This allows you to use the instrument for analyses other than the standard configurations mentioned previously. This flexibility also makes it possible for YSI to develop and introduce new chemistry analyses in the future without significant instrument changes.
Page 51 A variety of sample types can be analyzed with the 2700 SELECT. Generally, the only sample preparation that may be required is dilution of the sample to bring the substrate concentration within the linear range of the instrument. (see Section 1.4 for the working range of each chemistry).
Page 52 You may want to access the print setup command in your 2700 SELECT menu. From Run or Standby mode, press [MENU], then select [5] for PrntSetup. From Main Menu display, press [MENU], select [2] for Setup, then select [5] for PrntSetup.
Page 53 Calibration Method (2-CalMethod). In most chemistry setups, you will select “One Station”, and the calibration station # will be “Station #1” (Calibration Well). However, you have options to configure this in other ways. » Select One Station, but assign Cal Station #2. Station #2 is the Test Tube Holder Station.
Page 54 30 sec Note: See Appendix A if concentration unit conversion is required. * For applications requiring linearity performance to 25.0 g/L, YSI 2777 (25.0 g/L dextrose) may be used as a linearity standard provided the sample size is 10 µL.
Page 55 Special Considerations: » If sample dilution is required, use reagent water or 2357 buffer. » D-Lactate is not a substrate for L-Lactate Oxidase. Therefore, the 2700 SELECT cannot directly measure D-Lactate. If you have a known racemic mixture of lactates,...
Page 56 This is a direct reading of sucrose in solution at the enzyme sensor. Three enzymes are co- immobilized in the YSI Sucrose Membrane: Invertase, Mutarotase, and Glucose Oxidase. α-D-Glucose + (Fructose) Sucrose + H Invertase α-D-Glucose β-D-Glucose Mutarotase β-D-Glucose + O + D-Glucono-δ-Lactone...
Page 57 They may interfere by producing artificially high lactose readings. » The enzyme membrane integrity test involving the use of YSI 2363 Potassium Ferrocyanide solution is not informative since ferrocyanide exists in the 2705 system...
Page 58 Oxidase is immobilized in the enzyme membrane. Ethanol + O + Acetaldehyde Alcohol Oxidase System Buffer YSI 2787 Calibrator Std YSI 2390 (Solution A, 2.00 g/L) Linearity Std YSI 2390 (Solution B, 3.20 g/L) Black Probe YSI 2786 Alcohol Oxidase Membrane White Probe n/a * 10 µL (for best linearity)
Page 59 End Point 30 sec * For applications requiring linearity performance to 25.0 g/L, YSI 2777 (25.0 g/L dextrose) may be used as a linearity standard provided the sample size is 10 µL. ** If you have a dual channel unit, either Black or White, or both Black and White probes can be configured for this chemistry.
Page 60 For example, to express results as a choline hydroxide value, program the calibrator value as 204 mg/L when using the YSI 2772 Choline Standard calibrator solution. Your results will now reflect the concentration of choline hydroxide in the sample.
Page 61 They may interfere by producing artificially high galactose readings. » The enzyme membrane integrity test involving the use of YSI 2363 Potassium Ferrocyanide solution is not informative since, ferrocyanide exists in the 2705 system buffer.
Page 62 If so, it can also accumulate in the 2700 SELECT sample chamber. A small amount of sodium azide added to the system buffer will inactivate the catalase. Concentrations around 5 mg/L sodium azide are appropriate.
Page 63 To express results as an MSG value, change the glutamate units to a w/v expression, and enter one of the following: 936 mg/L, 0.936 g/L, or 0.094 %. Use YSI 2755 Calibrator Standard (5.00 mmol/L Glutamate) to calibrate the instrument. Sample results will then reflect MSG concentration.
Page 64 # This is a direct reading of glutamine in solution at the enzyme sensor. Two enzymes are co- immobilized in the YSI Glutamine Membrane: Glutaminase and Glutamate Oxidase. L-Glutamine L-Glutamate + NH Glutaminase + α-Ketoglutarate + NH L-Glutamate + O...
Page 65 Oxidase is immobilized in the enzyme membrane. Methanol + O + Formaldehyde Alcohol Oxidase System Buffer YSI 1579 Calibrator Std YSI 2726 (Solution A, 1.00 g/L) Linearity Std YSI 2726 (Solution B, 2.50 g/L) Black Probe YSI 2725 Methanol Membrane White Probe n/a * 15 µL (for best linearity)
Page 66 $ +## You must have a dual channel version of the 2700 SELECT in order to perform this measurement. Refer to the sections above on DEXTROSE and L-LACTATE for theoretical and special considerations. Then follow the instructions below to set up your instrument for simultaneous determination.
Page 67 % + You must have a dual channel version of the 2700 SELECT in order to perform this measurement. Refer to the sections above on DEXTROSE and SUCROSE for theoretical and special considerations. Then follow the instructions below to set up your instrument for simultaneous determination.
Page 68 , and appropriate dilutions of the sample to first measure free dextrose, then treat one sample with invertase and analyze for dextrose liberated from the sucrose. Contact YSI Customer Service, or your dealer representative for an application note explaining this approach in detail.
Page 69 & # +# You must have a dual channel version of the 2700 SELECT in order to perform this measurement. Refer to the previous sections on L-GLUTAMATE and L-GLUTAMINE for theoretical and special considerations, then follow the instructions below to set up your instrument for simultaneous determination.
Page 70 MENU Mode you must exit RUN or STANDBY modes. Figure 5.1 2700 SELECT Software Structure To exit RUN Mode press [RUN], then confirm your exit by pressing [2] for Yes, then [ENTER]. The instrument will next display the message shown below.
Page 71 Figure 5.2 YSI 2700 SELECT Menu Flow Chart REMEMBER: You move through the 2700 SELECT menus by selecting options on the display. You can press [MENU] to get back to the Main Menu, and [0] to back up to the previous display.
Page 72 Service functions are primarily used during initial setup and anytime reagent changes are made. Both Cal Pump and Buffer Pump can be primed using this menu level. In addition, Sipper alignment and Stir Bar speed adjustment can be performed from this level. Use the display messages and text below to learn the service functions.
Page 73 # $ " % " & & " '$$ " "! & If you press [5] for Monitor, you will access the functions used to align and prime the YSI 2730 Monitor and Control Accessory. Refer to the YSI 2730 Monitor and Control Accessory User’s Manual for details.
Page 74 Once set, the parameters are maintained in memory. In the event of power loss, the 2700 SELECT has battery backup to preserve this information.
Page 75 Enter date and time as required Month> 2 Enter date and time as required Date> 14 Enter date and time as required Hour> 17 Enter date and time as required Minute> 22 Select and/or confirm the format you desire for printed dates; (month/day/year) or (day/month/year).
Page 76 The configuration option allows operation in either a point-to-point environment or in a multidrop environment with an address (ID) assigned to your 2700 SELECT unit(s). The default configuration is Non-multidrop. If you press [2] for Multidrop, you will see the following.
Page 77 Enter this number in memory. It will be printed for certain report formats. NOTE: YSI uses alpha-characters in the serial number. We recommend that you press [SPACE] for the first alpha-character and omit the last two. Example, 98B01234AB can be entered as 98 01234.
Page 78 The default location is Station #1 (Cal Well). The choices are #1 and/or #2. Number 2 is the Test Tube Holder location. With Accessories such as the YSI 2710 Turntable and YSI 2730 Monitor and Control Accessory, stations #4 and #5 are also options.
Page 79 Press [1] for Next chemistry to scroll through the choices in the menu. The choices are L- Lactate, Sucrose, Lactose, Galactose, Peroxide, Ethanol, Choline, L-Glutamate, L-Glutamine, Methanol, none, and Dextrose. The Black probe default chemistry is L-Lactate. Press [ENTER] to confirm and continue to the unit of measurement parameter. The choices are grams/liter (g/L), millimoles/liter (mmol/L), milligram/liter (mg/L), which is often referred to as parts per million (ppm), and percent (weight/volume).
Page 80 Enter WHITE end point in seconds > 30 Press [ENTER] to confirm and return to Measurement parameter setup. Measurement parameter setup 1-SampleSize 2-CalMethod 3-Black 4-White You have now completed MeasParameter setup. Press [0] to return to the Select setup level. Select setup: 1-General 2-MeasParameter 3-RunMode 4-Report 5-PrntSetup 6-Default Press [3] for RunMode.
Page 81 Test Tube Holder (#2) and sample from the Cal Well (#1). If you have the YSI 2710 Turntable or YSI 2730 Monitor and Control Accessory, stations #4 and #5 are also available. See the appropriate manual for assignments.
Page 82 $ ! & #&$# " ' $$ $ 3 & ' These selections are provided as part of the overall concept of the 2700 SELECT, flexibility. You may have an application where 5% precision is acceptable if you can guarantee that the instrument will not initiate a calibration at a critical sampling time.
Page 83 Autocal setup: 1-Temperature 2-Time 3-Sample 4-Cal shift 5-Sample error Press [2] for Time. Enter autocal time interval in minute > 15 Confirm value shown or enter and confirm any number of minutes from 0 to 30000. Autocal setup: 1-Temperature 2-Time 3-Sample 4-Cal shift 5-Sample error Press [3] for Sample.
Page 84 Select cal report format: Brief 1-None 2-Brief 3-Detail Report Format refers to the information that will be printed each time the 2700 SELECT runs a sample or a calibration. The default settings are "Brief". Detail report formats are typically used when you want to study probe currents and temperature changes with newly installed reagents or enzyme membranes.
Page 85 YOU HAVE NOW COMPLETED THE SETUP MENU OF THE SOFTWARE. As you gain more familiarity with the 2700 SELECT menus, the setup will not seem so complicated. You will learn to "jump in and out" of the menus quickly, sometimes not even needing to exit RUN or STANDBY mode to make a change.
Page 86 Sipper Pump and sense the Test Tube Holder position. Follow the display prompts and information below to learn about the 2700 SELECT menu diagnostics mode. As before, you will often need to press [0] to return to a previous menu level.
Page 87 As discussed in the Introduction to this section, you must exit RUN Mode or STANDBY Mode to gain complete access to the diagnostic menu functions. To exit Run or Standby modes, press the appropriate key ([RUN] or [STANDBY]), then follow the prompts to exit. You will now be in Main Menu mode and the display will appear as shown below.
Page 88 Press [6] to initiate repeated motions in the same plane. We refer to this as" Exercise". The motion (horizontal or vertical) is determined by the last command you chose. The instrument counts the number of cycles and displays them as shown below. When you press a key to terminate the exercise mode, the printer records the activity and number of cycles completed.
Page 89 The display will now update to give two new selections as shown below. Sipper pump diagnostic 1-Home 2-Aspirate separator 3-Exercise Press [2] for Aspirate separator. A short retraction of the plunger should occur. This simulates the aspiration of an air bubble to isolate sample from the buffer stream. The display updates to read the following.
Page 90 Press [0] to return to the five diagnostic selections. NOTE: Unless the 2730 Monitor and Control pump is installed, 4-ExtPump will give no response, Refer to the YSI 2730 Monitor and Control Accessory User’s Manual for details of external pump operation.
Page 91 B:LAC 11.77 nA W:DEX 23.17 nA 1-Flush 2-Calibrator 3-Sample Next, press [1] for Flush to clear the calibrator solutions from the Sample Chamber. The flush will take about 30-40 seconds. If the baseline has not returned to levels you previously observed, you may want to initiate a second flush cycle.
Page 92 Hit keys - Hold [CANCEL] to exit Key> [RUN] released To exit the keypad test, press and hold down the [CANCEL] key. Hit keys - Hold [CANCEL] to exit Key> [CANCEL] held down The following message appears after you exit the keypad test. I/O diagnostic 1-Keypad 2-Display 3-Printer Press [2] for Display.
Page 93 Press [0] to return to the five diagnostic selections. Select diagnostic 1-Motor 2-Pump 3-Probe 4-I/O 5-Sensor Press [5] for Sensor. You may select one of three categories of sensors: temperature, fluid level or detection, and optosensors for a variety of motion status signals. Sensor diagnostic 1-Temperature 2-LevelSensor 3-OptoSensor Press [1] for Temperature.
Page 94 If the Sipper Pump plunger is at "home", i.e. extended, the SipperPump message should be ON; if the plunger is in any position other than home, the message should be OFF. If there is a test tube in the Test Tube Holder and it is in a vertical orientation, the "TestTube" message should be ON;...
Page 95 5-26...
Page 96 The sensor technology of the YSI 2700 SELECT is based on the principles conceived by Dr. Leland Clark of Children’s Hospital Foundation, Cincinnati, Ohio. The immobilized enzyme membrane was invented by YSI and is covered by U.S. Patent 4,073,713. This sensor technology has been used successfully since 1975 in the YSI 23A Blood Glucose Analyzer;...
Page 97 The face of the probe, covered by the membrane, is situated in a buffer-filled sample chamber into which a sample is injected. Some of the substrate diffuses through the membrane. When it contacts the immobilized oxidase enzyme, it is rapidly oxidized, producing hydrogen peroxide. See Reaction 1, using glucose as an example.
Page 98 The 2700 SELECT fluid and electromechanics system is schematically described below in Figure 6.2. Figure 6.2 2700 SELECT Fluid System...
Page 99 ! The 2700 SELECT monitors the probe baseline current for nA activity and stability. If an unstable baseline is detected, the buffer pump will continue to flush the sample chamber with buffer. When a stable baseline is established, an automatic calibration is initiated.
Page 100 The sensors’ net current for a calibration is displayed and printed. An unstable calibration is displayed and printed as a "cal shift". While establishing a stable calibration, the 2700 SELECT will run 5 calibrations before the error is displayed.
Page 101 The capacitive method depends on close proximity between the bottles and the metal chassis of the 2700 SELECT. The bottles should always rest on the metal floor inside of the instrument. It is also very important that the level sensor cables do not contact the metal...
Page 102 STANDBY Mode, where system power is on, but only a few maintenance functions occur. This conserves reagents during extended periods of inactivity. The 2700 SELECT also has menu selections for service, setup and diagnostics. The Main Menu of the software therefore provides three options: RUN, STANDBY, and MENU.
Page 104: Preventive Maintenance
Empty the Waste Bottle If the 2700 SELECT is used for medical research or biological testing, dispose of the waste bottle contents in a manner suitable for biohazardous waste. The reagents used in the 2700 SELECT are non-toxic and, unless otherwise specified, consist of a phosphate salt buffer with small amounts of preservatives.
Page 105: Check For Leaks
Please select instrument mode [RUN] [STANDBY] [MENU] Select instrument function 1-Service 2-Setup 3-Diagnostic Select service: 1-Sipper 2-Buffer 3-Cal 4-Stir speed 5-Monitor 6-Turntable If the calibrator bottle cap assembly needs cleaning, make sure it is thoroughly rinsed and dried before it is attached to a fresh bottle of calibrator solution. If you are calibrating from a test tube at the Test Tube Holder Station (#2), evaporation is a major concern.
Page 106: Clean Up Spills
Section 7.3 to disassemble the chamber and clean the base. Check that the drain tube fits tightly over the stainless steel fitting at the bottom of the chamber. If loose, replace the tubing. See Appendix B for the part number of the 2700 SELECT preventive maintenance kit. Daily Operational Checks To verify proper instrument performance, perform the daily operational checks described in Section 3.3.
Page 107 It is necessary to periodically clean both the sample chamber and the base plate it sets on. Move the Sipper to the manual sample position. From the Main Menu press [MENU]. Next press [3] for Diagnostic, then press [1] for Motor, then press [1] for Sipper motor. Press [1] for Up, to raise the Sipper from the Sample Chamber, then press [4] for Clockwise to move the Sipper over to the Manual Sample position.
Page 108 Clean up any salt deposits or fluid on the base plate. Be sure that the base plate and all other parts are dry. Refer to Figure 7.1 and reassemble the chamber. Remember to return the stir bar to the chamber and be sure to install the chamber seal O-ring. Insert the probes into the chamber, installing new membranes (see Section 2.4) or O-rings as necessary.
Page 109: Sensor Maintenance
With normal use, enzyme sensors may become fouled and cease to operate in a normal mode. A fouled sensor’s output current will decrease and calibration may become unstable. Since the severity of fouling will vary, listed below are methods of cleaning which should be matched to the cleaning needs.
Page 110 & The buffer and calibrator pumps in the 2700 SELECT are peristaltic tubing pumps. The tubing life depends on instrument usage. The pump tubes should be replaced at least every 6 months, more frequently if necessary. Other tubes become loose at the fittings and should also be replaced every 6 months.
Page 111 Figure 7.3 Pump Tubing Installation Make sure that the correct length of tubing is sticking out of the pump (1 to 1½″ [2.5 to 3.5 cm] of the large diameter tubing on the left side of the buffer pump), then rotate the roller assembly while pushing the tubing down into the pump ahead of the roller.
Page 112 Slot Rear Housing Roller Assembly Tubing Front Housing Tube Bushing Figure 7.4 Pump Assembly When all tubes are properly connected, see Section 2.6 to readjust the Sipper and prime the calibrator and buffer systems. The Sipper can be damaged if it is not properly aligned or if its alignment is disturbed. If your Sipper needs replaced, remove the screw that mounts it to the sipper arm, disconnect the tubing and remove the Sipper.
Page 113 . It may be necessary to replace the fuses in the 2700 SELECT. New fuses may be purchased from YSI or obtained from many local electrical component suppliers. Be sure to obtain the correct fuse rating as indicated below. UNPLUG THE INSTRUMENT FROM THE MAINS SUPPLY.
Page 114 / Replace the Sipper Pump seals every 6 months. Heavy usage may warrant more frequent replacement. Disconnect the tubings to the Sipper Pump. Remove the Sipper Pump head from the instrument wall (see Figure 7.5). Figure 7.6 Sipper Pump Head Removal Pull the white pump base from the clear pump housing.
Page 115 Figure 7.7 Sipper Pump Seal Replacement Reassemble the pump, position the plunger as shown in figure 7.7 and install it back on the instrument. Figure 7.8 Sipper Pump Plunger Position !"#"$% 0) 12&3) 4″' '5.6&( () ))7 7-12...
Page 116 Apply Lubriplate 105 grease (supplied in YSI 2788 Preventive Maintenance Kit) to two or three threads of the Acme screw. Normal operation will distribute the grease along the length of the screw.
Page 117 7-14...
Page 118 » Use the troubleshooting chart in this section to assist you in identifying the problem, then use the chart to guide you to a corrective action. If the problem cannot be resolved, contact YSI Customer Service (address and phone information in Appendix F). When communicating with service personnel, please indicate the serial number of the instrument.
Page 119: Calibration Report
Refer to Appendix D to learn more about report format options for the 2700 SELECT. For troubleshooting, or even daily log information, the "detail" report format is preferable. The Detail Report provides a complete description of the sensors for a calibration or sample.
Page 120 The default setting is 2%. That is, if the shift is greater than 2%, the 2700 SELECT performs another calibration. Up to 5 successive calibration shifts are permitted before the 'Unable to calibrate' error is displayed. You may select Cal shift values that better suit your application.
Page 121: Possible Cause
SYMPTOM: ERROR: Baseline POSSIBLE CAUSE: Pinched, leaking or disconnected tube. ACTION: Fix or replace tubing. SECTION: POSSIBLE CAUSE: Sipper misaligned. ACTION: Check Sipper alignment. SECTION: POSSIBLE CAUSE: Buffer pump not operating properly. ACTION: Replace tubing. SECTION: POSSIBLE CAUSE: Stir bar not in chamber. ACTION: Disassemble chamber and reinstall stir bar.
Page 122 SYMPTOM: ERROR: Empty Buffer Bottle (Continued) POSSIBLE CAUSE: Level sensor cable not plugged in. ACTION: Plug in cable. SECTION: ERROR: Empty Calibrator Bottle SYMPTOM: POSSIBLE CAUSE: Low calibrator level. ACTION: Install new calibrator. SECTION: POSSIBLE CAUSE: Level sensor cable not plugged in. ACTION: Plug in cable.
Page 123 Replace sipper ground cable. SECTION: POSSIBLE CAUSE: Intermittent sipper cable. ACTION: Replace sipper cable. SECTION: See the 2700 SELECT SERVICE MANUAL SYMPTOM: ERROR: Test Tube Interference POSSIBLE CAUSE: Sample tube holder not in position. ACTION: Reposition and repeat sample. SECTION:...
Page 124 POSSIBLE CAUSE: Printing on wrong side of paper. ACTION: Install paper correctly. SECTION: POSSIBLE CAUSE: Using wrong type of paper. ACTION: Install correct thermal paper (YSI 2751). SECTION: SYMPTOM: Fail FCN Test POSSIBLE CAUSE: Damaged or old membrane. ACTION: Replace membrane.
Page 125 SYMPTOM: Fail Linearity Test POSSIBLE CAUSE: Probe assignment incorrect. ACTION: Make correct assignment. SECTION: POSSIBLE CAUSE: Damaged or old membrane. ACTION: Replace membrane. SECTION: POSSIBLE CAUSE: Contaminated or old calibration or linearity standard. ACTION: Repeat test with new standards. SECTION: POSSIBLE CAUSE: Assigned concentration range beyond practical limits.
Page 126 2.03 and up. In sections below you will learn about communications handshaking, software setup, command structure, communication modes and data base format. Note: The term "2700 SELECT" has been shortened to "2700" in many sentences and figures below. They are synonymous. The 2700 communicates with a host computer through its RS-232 serial port, acting as a DTE.
Page 127 Hardware Handshaking Signals. The four handshaking signals used to control the data flow are RTS/CTS and DTR/DCD. The 2700 drives RTS when it wishes to transmit and waits for CTS before transmitting each character. Likewise, the 2700 asserts DTR when it is ready to receive data and receives only when DCD is asserted.
Page 128 In this grammar, < addr> is a single byte (binary) node address that you assign to the 2700 SELECT. You make the assignment via the instrument keypad. The 2700 SELECT only responds to commands with the < addr> field that matches the assigned address.
Page 129: Error Codes
Error Codes Code 1 a) 2700 is not in remote control mode. This condition generally indicates that you invoked the TN1 command (enter Run Mode remotely) when the 2700 was not in remote mode. You must first successfully invoke TR1 (turn on remote control mode).
Page 130 The 2700 can be placed in one of two communications modes: Result Reporting Mode or Remote Control Mode. The selection between modes is made by the remote host. The default mode at power-up is Result Reporting Mode. 2700 Data Base. The 2700 keeps a data base of 32 sample results and 1 calibration result in its battery-backed RAM (random access memory).
Page 131 In single channel units, this will not be a concern. In Figure 9.4, several examples show how information for a dual channel 2700 SELECT may appear when transmitted. Again, the ruler line is for reference only and does not appear on the record.
Page 132 0000 <cr><lf> Figure 9.4 2700 SELECT Data Report Format NOTE: The specific information above is not intended to appear realistic, but rather to account for all characters and spaces in the report format. See Figure 9.5 for specific field location...
Page 133 The fields of the data transmission records are more specifically defined below in Figure 9.5. A ruler line is used simply to help you identify the offset and field widths in the report. We have used the following symbols to depict changes from early 2700 software vs versions 2.41 and higher.
Page 134: Report Database
Command Description. For quick reference, the communications commands are divided into five functional groups: process, report database, database management, control and report system information. For details on process commands, see "Remote Control Commands", later in this section. PROCESS Process calibration Process sample from calibration well (Station #1) Process sample from test tube holder (Station #2) Process turntable batch (Station #4)
Page 135 Common Commands. The commands below can be invoked in either Remote Control Mode or in Result Reporting Mode, unless otherwise stated. Available commands are those in the Report Database and Report System Information categories above. When Report Status (RY) is invoked, the command obtains the 2700 status. The status includes communications mode, sample result status, calibration result status, machine status and remote command status.
Page 136 When Report Last Transmission (RX) is invoked, the last transmission of the 2700 is sent to the host. It is used when the response received by the 2700 contains an unknown character, is not in the correct format, or whenever in doubt due to communications error. Host to 2700: Report last transmission 2700 to Host:...
Page 137 Remote Control Commands. The commands outlined above in the "Process" category can be invoked only in Remote Control Mode. If the system is not in the Remote Control Mode, you must first invoke the Turn Remote Control On (TR1) command. Note: For each command from Host to 2700, the 2700 sends back an acknowledgment (A) or an error code.
Page 138 Host to 2700: Exit Run Mode and switch to Standby Mode or Menu Mode. 2700 to Host: Acknowledge. 2700 exits Run Mode. 2700 is not in Remote Control Mode. 2700 is busy in Run Mode. When you invoke TN1 (Enter Run Mode), the instrument enters Run Mode from Standby or from “Menu Mode”.
Page 139 When you invoke the Process Sample (PS) command, the Host commands the 2700 to sample from one of four locations depending on the specific command (PS1, PS2, PS4 or PS5). If the 2700 is running an autocalibration, calibration stabilization or baseline stabilization, the command is acknowledged and the sample is processed as soon as the instrument finishes the existing task.
Page 140 When you invoke the RM (Report Parameters) command the instrument reports to you the current system time, date and measurement parameters. For ease of deciphering this information, the report format is almost identical to that of the sample and calibration reports. The only difference is that the Error Field, beginning at column 62, contains additional information about the calibration parameters.
Page 141 Figure 9.7 to understand these setup parameters. The report is divided into several categories similar to "PrntSetup" ( Print Setup) in the 2700 SELECT menu option. Note, all fields in this report are right-justified.
Page 142 Abort Turntable Sample Command (PA). You may use this command to abort turntable samples in progress. Recall, you initiate the turntable sampling using the PS command. You cannot use the 2700 SELECT [CANCEL] key to abort the run, since the 2700 SELECT keypad is disabled in remote control mode.
Page 143 9-18...
Page 144 In the YSI 2700 SELECT menu selection procedure you have the option to assign units of concentration. You will find this in the Setup Menu under measurement parameters, black and white probes. There are default values set based on calibration solutions offered by YSI. Below is a table of unit conversions for these calibration solutions and other concentrations of interest.
Page 145 Example Conversions Beginning with 2.50 g/L dextrose, convert this to mg\L, then % and finally to mmol/L: 1. Multiply by unit conversion(s) 2. Cancel units common in “numerator” and “denominator” 3. Multiply numbers ? mg/L = 2.50 g/L = (2.50 g/L)(1000 mg/g) = (2.50)(1000) mg/L = 2500 mg/L ? % (w/v) = 2.50 g/L = (2.50 g/L)(0.1 L/dL) = (2.50)(0.1) g/dL = 0.250 g/dL = 0.250 % (Note: g/dL is g/100ml or percent)
Page 146 Linearity Test. Concentration Unit Conversion mg/L Chemistry (ppm) (w/v) mmol/L (g/mole) 0.47 0.047 4.54 Choline 0.45 0.045 4.32 (104) 0.43 0.043 4.10 9.45 9,450 0.945 52.5 Dextrose 9.00 9,000 0.900 50.0 (180) 8.55 8,550 0.855 47.5 3.36 3,360 0.34 73.1 Ethanol 3.20 3,200...
Page 147 0.01 0.29 (342) NOTE: Use the values from the preceding tables only when calibrating with the appropriate YSI calibrator solution: Choline (2772), Dextrose (2776), Ethanol (2790), L-Glutamate (2755), L- Glutamine (2736), L-Lactate (2776), Methanol (2726) and Sucrose (2780). See Section 3, Basic Operation, Daily Operational Checks for cross references.
Page 148 Methanol Membrane Kit 4/kit 2735 L-Glutamine Membrane Kit 4/kit 2754 L-Glutamate Oxidase Membrane Kit 4/kit 2771 Choline Oxidase Membrane Kit 4/kit Standards for 2700 Select YSI # Product Description Quantity 2726 Methanol Standard Kit: 1.00 g/L 180mL 2.50 g/L 120mL 2736 L-Glutamine Standard, 5.00 mmol/L...
Page 149 2730 Monitor and Control Accessory 2731 2730 Preventive Maintenance Kit 2751 Printer Paper (5 rolls/box) 2788 Preventive Maintenance Kit Contact YSI Incorporated or your authorized dealer representative for more information on ordering supplies and reagents for the YSI 2700 SELECT.
Page 150 You may print your 2700 SELECT instrument setup anytime by entering the Setup menu. You are not required to leave RUN Mode or STANDBY Mode to access this information. Main Menu display: Please select instrument mode [RUN] [STANDBY] [MENU] From Main Menu, press [MENU].
Page 151 XOFF char SampleReport : Brief Mode : Non-multidrop DateFormat : MM/DD/YY Address: Software revision: 2.56 GENERAL YSI 2700S - 98 01234 Radix mark : "." Thu 02/26/98 09:20:48 Level sensor : ON ------------------------ Cal Report : Brief SampleReport : Brief...
Page 152 When you setup your YSI 2700 SELECT from the Setup Menu, you have the option to print the calibration and sample results in "brief" or "detail" format. You also have the option not to print calibration values. Review Section 5.3, Setup, specifically choice 4-Report in the Setup selection menu.
Page 154 The Federal Communications Commission defines this product as a computing device and requires the following notice: This equipment generates and uses radio frequency energy and if not installed and used properly, may cause interference to radio and television reception. It has been type tested and found to comply with the limits for a Class A or Class B computing device in accordance with the specification in Subpart J of Part 15 of FCC Rules, which are designed to provide reasonable protection against such interference in a residential installation.
Page 156 The YSI Model 2700 Analyzer is warranted for one year from date of purchase by the end user against defects in materials and workmanship, exclusive of batteries. Within the warranty period, YSI will repair or replace, at its sole discretion, free of charge, any product that YSI determines to be covered by this warranty.
Page 157: Cleaning Instructions
Disposition costs will be the responsibility of the sender. When service is required, either at the user's facility or at YSI, the following steps must be taken to insure the safety of our service personnel.
Page 158: Packing Instructions
Packing Instructions 1. Clean and decontaminate items to insure the safety of the handler. 2. Complete and include the Cleaning Certificate. 3. Place the product in a plastic bag to keep out dirt and packing material. 4. Use a large carton, preferably the original, and surround the product completely with packing material.
Page 160 Figure G.1 Line Power Cord and Plug Wiring...
Page 162 Proper precautionary lab practices should be followed when handling biological hazards. Suggested cleaning and disinfecting solutions include: » Isopropanol 70% » Sodium hypochlorite, 5000 ppm free available chlorine (1:10 solution of household bleach) Decontamination Procedures Remove and discard all tubing. New tubing is provided in preventive maintenance kit. Empty waste bottle and wash with disinfecting agent.
Page 164 1. Users should not open the front access door during operation, internal components may be susceptible to ESD (electrostatic discharge). 2. Users are cautioned not to touch the sipper needle during operation, as discussed in the users manual. The sipper mechanism is equipped with contact-sensing circuitry for the purposes of fluid detection and human safety.
Page 165 1725 Brannum Lane Yellow Springs, Ohio 45387 USA 062521 937 767-7241 • 800 659-8895 • Fax 937 767-8058 A23781L Info@ysi.com • www.YSI.com July 2000 © 2000 YSI Incorporated...

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