The YSI 2900 printer may not print results because the paper or roll is jammed. To fix this, remove the paper cover and clear the obstruction. If the printer still does not advance, turn the printer off for 30 seconds, then turn it back on.

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Summary of Contents for YSI 2900

Page 1 YSI 2900 Series Biochemistry Analyzers Operations and Maintenance Manual...
Page 2: Table Of Contents
Table of Contents Introduction ............................ 5 Description ..........................5 User Features ..........................5 Models ............................5 2900 Series General Specifications ..................6 2960 Online Monitor Specifications ................... 6 1.5.1 Monitor ..........................6 1.5.2 Analog/Control ........................7 Safety ............................... 7 Important Safety Instructions ..................... 7 Explanation of Symbols ......................
Page 3 5.3.1 Create Batches ......................... 35 5.3.2 Load Samples ........................37 5.3.3 Start ........................... 39 5.3.4 Status ..........................39 Run Stat ........................... 39 Results ............................. 42 Online Monitor and Control......................42 2960 Installation........................43 Align Sipper ..........................46 Sample Interface........................46 6.3.1 Sterilization ........................
Page 4 Ethernet Port ..........................98 7.5.1 LAN (Shared Network Connection) ................... 98 7.5.2 Router (Private Network Connection) ................98 7.5.3 Accessing Stored Data...................... 99 Chemistry Setup ......................... 101 Sample Volume ........................101 Measurement Parameter Information ..................101 8.2.1 Choline ..........................102 8.2.2 Ethanol/Ethanol-HC ......................
Page 5 13.1.1 Shipping Instructions ....................152 13.1.2 Cleaning Instructions ....................153 13.2 YSI Factory Authorized Service Centers ................154 Notices ............................155 14.1 Declaration of Conformity ...................... 155 14.2 Declaration of Conformity - Australian and New Zealand Electromagnetic Compatibility ..156 14.3...
Page 6: Introduction
PERFORMANCE EVALUATION. The 2900 Series can be set up to measure up to 6 different analytes in a sample. The total number of analytes depends on the number of sample modules installed (up to two analytes per module) and the number of different buffers required (up to three buffers).
Page 7: 2900 Series General Specifications
2900 Series General Specifications Sample size ..........Adjustable from 10 to 50 microliters (aspirated volume), per module Response time ..........Enzyme Sensors • Sample results in 60 seconds (average) for one module • 100 seconds for 2 modules • 135 seconds for 3 modules •...
Page 8: Analog/Control
See Appendix for mains plug wiring and fusing instructions. 8. If the equipment is used in a manner not specified by YSI, the protection provided by the equipment may be impaired. WARNING: For RS232 or USB connection, equipment should be EN/CSA/UL 61010 or EN/CSA/UL 60950 approved only.
Page 9: Explanation Of Symbols
Explanation of Symbols Warning indicates that misuse of the instrument could result in WARNING death or serious injury to a person. Un avertissement indique qu'une mauvaise utilisation de l'instrument peut entraîner la mort ou une blessure grave chez une personne. AVERTISSEMENT Caution, consult accompanying documents.
Page 10: Getting Started
When you unpack your new 2900 Series for the first time, check the packing list to make sure you have received everything listed. Note that reagents for the 2900 Series are not packaged in the same carton as the instrument. If there is anything missing or damaged, call the dealer from whom you purchased the 2900 Series.
Page 11: Warranty Card
Warranty Card Please complete the Warranty Card and return it to YSI. This will record your purchase of this instrument in our computer system. Once your purchase is recorded, you will receive prompt, efficient service in the event any part of your 2900 Series should ever need repair.
Page 12 USB ports The USB ports allow a flash drive to be connected to the 2900 Series to download sample results or upgrade the instrument’s software. A USB port is located on the right side of the display. An additional USB port is located on the rear of the instrument. The rear port is used to connect the 2960 Online Monitor to the 2900 Series.
Page 13: Basic Setup
The RS232 connection is a standard DB9F connector. It is used to interface with the YSI 2901 Printer or a remote host. 4. Basic Setup The following list describes the basic steps necessary for sampling with the 2900 Series. 1. Install Bottle Racks 2. Connect Printer (optional)
Page 14: Install Bottle Racks
Install Bottle Racks 4.1.1 Right Side 1. Install the YSI 2938 Bottle Rack with Reagent Level Sensing onto the right side of the instrument by sliding the slots in the tray over the pins on the side of the instrument.
Page 15: Left Side (2950 Models Only)
2. If you are not using the reagent level sensing option, place the YSI 2937 Bottles on the left side of the instrument. 3. Then remove the packing material holding the tubing to the left side of the instrument.
Page 16: Connect Printer
Connect AC Power 1. Plug the power cord (included with the 2900 Series Analyzer) into the power receptacle on the back of the instrument, then into a properly grounded electrical outlet provided with a 15 or 20 Amp circuit breaker. The instrument will automatically adjust the voltage as needed.
Page 17: Connect 2960 Online Monitor
Connect 2960 Online Monitor If you will be using the 2960 Online Monitor, see Section 6.1 for instructions on how to connect it. Align Sipper It is very important that the sipper be accurately adjusted. WARNING: Keep your hands clear of the sipper while the instrument is in operation. 1.
Page 18 3. If the sipper is not centered, touch [Position] and use the arrow buttons to center the sipper. 4. Make certain the Sipper is centered, then touch  at the bottom right of the adjustment window. Sipper Adjustment Position Figure 4.3 5.
Page 19 7. Once the sipper enters the sample module without hitting the cone, touch [Depth] to set the sipper depth. The Select Location screen will appear. 8. Select [Module 1]. The tip of the sipper should be right at the top of the module. Use the arrow buttons to lower or raise the sipper until the tip of the sipper is even with the top of the module.
Page 20: Configure Instrument Chemistries
After you have aligned the sipper with all installed modules, touch the Interlock button and change it back to [On] to enable the safety interlocks. Configure Instrument Chemistries Before operating the 2900 Series, you must set the instrument parameters. 4.6.1 Assign Chemistries to Probes 1. From the main display, touch 2.
Page 21 4. The Probe A Membrane button will now show the chemistry you have selected. The screen also indicates which reagents should be installed. 5. To run a second chemistry in module 1, touch the Probe B membrane button. Only chemistries that can be run simultaneously with your selected chemistry will be displayed.
Page 22 NOTE: The default Sample Volume and Endpoint for the chemistries are also displayed. Use the default settings unless a particular application instruction specifies another value (see Section 8 Chemistry Setup for details). 7. Once you have selected one or two chemistries for the first module, proceed to the next installed module on your instrument, and repeat the procedure.
Page 23: Assign Reagents
Next Arrow 4.6.2 Assign Reagents Once you have selected all the chemistries you want to measure, touch the Next arrow on the right of the screen. The Assign Reagents screen will be displayed. Reagents needed are listed on the left of the screen. Bottle positions are listed on the right. Each reagent must be assigned to a bottle position.
Page 24 2. Pick your appropriate buffer and it will be assigned to the B1 button. Note that the selected buffer is now marked out in the list of reagents needed. 3. For additional buffer assignment, please repeat process. 4.6.2.2 Calibrators 1. Touch the [Cal 1A] button. The Select Calibrator screen appears.
Page 25: Prepare And Install Buffer Solutions
2. Pick the appropriate calibrator. 3. Select additional bottles until all reagents on the left side of the screen have been assigned and marked out. Prepare and Install Buffer Solutions Caution: To prevent possible damage due to an electrostatic discharge, do NOT touch the metal tips of the connectors located at the ends of the bottle leads.
Page 26: Prepare Buffer
4.7.1 Prepare Buffer 4.7.1.1 From powder concentrate: 1. Place about 500 mL of reagent water (distilled or deionized) into a 1000 mL flask or other clean container. 2. Add two packages of powder buffer concentrate and stir. 3. Add more reagent water until the total volume of solution is between 900 and 1000 mL. 4.
Page 27: Install Membranes And Ises
IMPORTANT: make every effort to avoid contamination of the lid and level sensor assemblies. Mark the date of installation on the label of the new bottle of YSI calibrator solution. Place the new bottle of calibrator in the bottle rack as indicated on the display.
Page 28: Ion Selective Electrodes
4.9.2 Ion Selective Electrodes The 2900 Series is shipped without the Ion Selective Electrodes installed in module 3. To install an ISE: 1. First remove the packaging material and the cap from the ISE (save the cap for later use).
Page 29 Retainer Figure 4.6 Figure 4.7 4. Place an O-ring into the end of each sleeve, pushing the O-ring gently so that it is secured by the sleeve. O-ring Figure 4.8 5. Install the reference electrode by screwing the probe retainer into the module (finger tighten only; do not overtighten).
Page 30: Prime The Fluid System
Reference—REF Ammonium—NH4 Potassium—K Figure 4.10 9. Install the top covers on the instrument. 4.10 Prime the Fluid System Please note that it may take from several minutes to more than an hour to initially stabilize the probes when setting up for the first time.
Page 31: Check Probe Currents
If you will be using the optional 21CFR Part 11 compliance features, enable 21 CFR Part 11 Mode (see Section 7.1.5.1 for details). 5. Running the Instrument Perform Daily Operational Checks To ensure that your 2900 Series is operating properly, perform the Membrane Integrity and Linearity checks on a daily basis before running samples.
Page 32: Enzyme Membrane Integrity Test
5.1.1 Enzyme Membrane Integrity Test Use YSI 2363 Potassium Ferrocyanide (FCN) Standard to determine if your enzyme membranes are structurally intact. 1. Pour small amount of FCN standard (1000 mg/dL) in a test tube or multi-well plate 2. Press the Run icon to process it as a sample.
Page 33 8. Touch the [Batch] button. 9. Select only the chemistries that require the FCN test. 10. To change the Batch Name from the default value of TestBatch- #, touch the [TestBatch- #] button. The keypad window will appear. 11. Type your new batch name and touch [DONE]. ...
Page 34: Linearity Test
5.1.2 Linearity Test 13. Pour small amount of linearity standard in a test tube or multi-well plate. 14. Touch additional sample locations to create new batch for the linearity tests. 15. For the daily linearity checks, select only the chemistry that corresponds to the linearity standard in that sample location.
Page 35 Select a sample 23. Touch a chemistry to show details Select to show details 24. Listed in table 6-1 below are the recommended FCN limits. a. Values less or equal to FCN limits indicate integral membranes b. Values greater than FCN limits indicate membrane structural failure c.
Page 36: Sample Preparation
Sample Preparation A variety of sample types can be analyzed with the 2900 Series. Generally, the only sample preparation that may be required is dilution of the sample to bring the substrate concentration within the linear range of the instrument. (see Section 8 Chemistry Setup for the working range of each chemistry).
Page 37 Arrows 2. Touch [New] and choose from the selection of supported racks and plates. Alternatively, use the arrows to scroll through the saved racks and plates until you find the type that you are using. 3. You may rename the rack/plate by touching its ID. 4.
Page 38: Load Samples
14. Touch [Close] when all batches are created. 15. To save your plate configurations to a flash drive, touch [Export]. 16. Select the plates you want to export, and then touch [Export]. 17. Previously exported plates can be imported later using the [Import] button. 5.3.2 Load Samples 5.3.2.1 R24 and P6-P96 Racks/Plates 1.
Page 39 Figure 5.2 5.3.2.2 R4 or R8 Tube Racks 1. Open the front door of the instrument. 2. Insert the R4 or R8 tube rack into the cavity just inside the front door. R8 Tube Rack Figure 5.3 3. Insert your sample tubes into the tube rack you installed.
Page 40: Start
Figure 5.4 5.3.3 Start Touch to run the current batches. The 2900 Series will calibrate as required and run the batches. 5.3.4 Status 1. Touch the Status tab. 2. Then select a module to view its status. Select module to view status Virtual Printer 3.
Page 41 1. Touch the Run Stat tab 2. Place the sample in Station 2: a. Insert your sample tube into the tube holder (Station 2) from below the spring clip b. Slide it up all the way until it rests below the notch at the top. The test tube holder accepts tubes sizes up to 16x100mm.
Page 42 5. Touch  to return to the Run Stat screen. 6. Touch to run the Stat sample at Station 2. If a Station 1 batch is in progress, the Stat sample will run as soon as the required module(s) is available. 7.
Page 43: Results
6. Online Monitor and Control The YSI 2960 Online Monitor and Control System allows “on-line” monitoring and control of sterile systems over long periods of time without contamination. It also provides an alternative means of interfacing the 2900 Series with external measurement/control systems.
Page 44: 2960 Installation
1. Connect the small end of the supplied USB cable to the mini USB socket on the back of the 2960. 2. Connect the large end of the USB cable to the USB socket on the back of the 2900 Series.
Page 45 4. Connect the cable from the power supply to the power socket on the back of the 2960. 5. Using a 3/32” Allen hex wrench, remove the screw from the right rear of the 2900 Series deck as shown below.
Page 46 8. Connect the larger diameter waste tubing from the fitting on the left side of the 2960 Online Monitor to the waste fitting on the side of the Monitor Sample Cup inside the 2900 Series. 9. Run the larger diameter waste tubing from the fitting on the right side of the 2960 Online Monitor to an appropriate...
Page 47: Align Sipper
When configured appropriately the 2960 automatically samples a bioreactor, process stream, or other suitable sample source. Since color, turbidity, optical density and many other physical factors do not affect the YSI enzyme biosensor, filtration and/or dilution may not be necessary and the 2960 may draw the sample directly. If cell loss is a concern, or if high cell density is expected, a filtration device (e.g., tangential flow filter) which separates broth and cells may be...
Page 48: Sterilization
From Antiseptic From Sample source Figure 6.6 If you are using an antiseptic, connect the tubing from the inside slot of the 2960 valve to your antiseptic container. Typical solutions might include 1% sodium hydroxide or 0.25% hypochlorite in reagent water. Even if you are not using an antiseptic solution, the solenoid valve still switches to the antiseptic position and air or fluid is pumped through the antiseptic line during sample aspiration.
Page 49: Pump Control Outputs
Analog Output Ready Ack\ Figure 6.7 6.4.2 Pump Control Outputs Three discrete outputs are provided on the 2960 which are intended to control external pumps. The means of control is on or off only, no intermediate states are supported. The three outputs are labeled Feed Pump A, Feed Pump B, and Filtrate Pump.
Page 50: Name
3. When configuring the monitor for the first time, touch the [Edit] button to edit the default monitor configuration. To add additional configurations in the future, touch [New] to create a new configuration. 6.5.1 Name 4. Touch the [Name] button and enter a name for this configuration.
Page 51: Flow Rate And Purge Time
6.5.2 Flow Rate and Purge Time The flow rate and purge time settings must be such that a sufficient volume of sample is pumped during each cycle to completely purge the Monitor sample cup and system tubing. This is especially important when an antiseptic is used since the antiseptic may damage the enzyme membrane if aspirated by the sipper.
Page 52: Filtrate Pump
7. Touch the [On] button and change it to [Off] if you are not using and antiseptic solution. 8. Touch [Save] to save your change and return to the Edit Configuration screen. 6.5.4 Filtrate Pump The filtrate pump control output from the 2960 is provided to control a user-supplied external pump for pumping the sample from its point of origin to the 2960.
Page 53 When in the monitoring mode and when the 2900 Series is stabilized in terms of calibration drift, you may elect to disable autocalibrations related to time and number of samples and use the PreCal option only.
Page 54 14. Touch the Chemistry [None] button and select the chemistry you are monitoring. 15. Touch the Units [g/L] button and select the units for this chemistry. 6.5.5.4 Analog Output 16. Touch the DAC [None] button to enable the analog output for this probe. 17.
Page 55 6.5.5.5 Control Type 20. See Section 6.8 Control Setup for setting up the control function. 21. Touch [Save] to save your Probe A changes and return to the Edit Chamber 1 screen. Select Probe 22. To monitor an additional chemistry, touch the Probe B [Edit] button. 23.
Page 56 25. Touch [Save] to save your Chamber 1 changes and return to the Edit Configuration screen. 26. Touch [Save] to save your Configuration and return to the Select Configuration screen. 27. Touch [Select] to choose the selected configuration and return to the Monitor screen.
Page 57: Start Monitor
Start Monitor 1. Once you have setup and selected your monitor session, touch 2. Enter a name for this specific run, then touch [DONE]. 3. The monitor session will start. The instrument will calibrate the required probe(s) if necessary, or if Precal is enabled, then run the first monitor sample.
Page 58: Manual Sample During Monitor Session
Once the sample has run, it will be displayed on the graph: Sample Results Units Reset Zoom buttons Use zoom and reset to adjust the graph size. Touch the Units “g/L” to change the displayed monitor units. 6.6.1 Manual Sample during Monitor Session A plate/rack or Stat sample may be run during a monitor session provided there is sufficient time between monitor samples.
Page 59: Stop Monitor
This will initiate a monitor sample and reset the time until the next sample. Stop Monitor To stop the current monitor session, touch Control Setup The 2960 offer two types of control, Proportional-Integral-Derivative (PID) control or Threshold. PID control attempts to maintain the sample source at a user defined set point by adding an amount of feed stock proportional to the error.
Page 60 2. Touch [New] to create a new monitor configuration, or select an existing configuration and touch [Edit]. 3. Touch the Module 1 [Edit] button. 4. Touch the [Edit] button for the probe you want to control.
Page 61 5. Touch the Control Type [None] button. 6. Select the type of control for this channel, [PID] or [Threshold], and follow the corresponding instructions in the section below. 6.8.1.1 PID For proportional control, select [PID] as the Control Type. Setpoint Touch the Setpoint [10.00g/L] button and enter the concentration at which the analyte will be regulated.
Page 62 Error Direction [Under] means that the user wishes to regulate to the set point in an environment where the analyte is being consumed and as such tends to fall under the set point. In this circumstance the control algorithm will attempt to regulate by making additions of correction feed stock (which contains the analyte) to the controlled volume.
Page 63 Touch the Average Reactor Volume [10.00] button and enter the average volume of your reactor. Touch the Delivery Rate [10.00L/min] button and enter the flow rate of your feed pump. Touch the Maximum Rate of Change [10.00g/L/min] button and enter the maximum rate of change expected during the monitor session (maximum rate of consumption of the measured analyte during the run).
Page 64 Manual TPU Calculation Touch [Manual] to enter the TPU manually. Manual allows you to enter the TPU value that you calculated yourself. Touch the TPU [10.00 sec/g/L] button and enter your manually calculated TPU value. Touch [Save] to save changes and return to the Edit Probe screen.
Page 65 Feed Pump line Touch the Feed Pump Line [None] button and select the 2960 feed pump output line that your external feed pump is connected to. The default feed pump output is Active High (+5V). Touch the Feed Pump Line [High] button to change it to [Low] if your feed pump requires an Active Low signal (0V) to turn on.
Page 66 Touch [Save] to save changes and return to the Edit Configuration screen. Touch [Save] to save changes and return to the Select Configuration screen. Touch [Select] to choose the selected Configuration and return to the Monitor screen. 6.8.1.2 Threshold For simple threshold alarm, select [Threshold] as the Control Type.
Page 67 Touch the Setpoint [10.00g/L] button and enter the threshold value. Set the Error Direction to trigger when the measured analyte value goes [Under] the threshold or [Over] the threshold. Touch the Feed Pump Line [None] button and select the 2960 output line that your external alarm/pump is connected to. The default output is Active High (+5V).
Page 68: Print Monitor Configuration
Print Monitor Configuration Touch the Print Monitor Configuration button to print the current monitor configuration. The configuration can then be reviewed on the YSI 2901 Printer or from the Status tab: >SITINI ONLINE SAMPLER < > CONFIGURATION REPORT < Config Name: GLUCOSE CON...
Page 69: System Configuration
7. System Configuration Settings Touch [Settings] to display the global settings screen as shown below. Settings include System, Display, and Scheduler. NOTE: If 21 CFR Part 11 mode is enabled, only an Administrator can access Settings. 7.1.1 System Touch the [System] tab (if not already selected). The System tab is used to select the type of sample and calibration reports, enable sipper fluid detection, and print the configuration.
Page 70 Set the Communications Port to [Printer] when using the optional YSI 2901 Printer. The RS232 Communications Port can also be used to connect the 2900 Series to a remote host. Set the Communications Port to [Remote Commands] to enable the remote host to send commands to the 2900 Series.
Page 71: Fluid Detection (Bottles)
Touch the Dynamic Fluid Depth [On] button and change it to [Off] to disable sipper fluid detection and use fixed depth at sample stations. Touch the “When no fluid detected” button and select the sample fluid detection mode you prefer: [Error] Produce an error when no sample is detected (no sample result).
Page 72: Display
To change bottle Fluid Detection, touch the button for the bottle you wish to change. If the 2938 or 2936 Bottle Trays with Reagent Level Sensing are not installed, turn off the bottle sensors. NOTE: When bottle sensors are off, check bottle fluid levels regularly to prevent waste fluid from backing up into sample modules.
Page 73 Using a stylus, touch and hold the center of the red flashing that appears at each corner of the display until appears. After you have held the in all four corners, the touch panel is calibrated. Touch screen calibration can also be initiated by touching anywhere on the Initialization screen while the instrument is powering up.
Page 74: Auto-Cal Settings
YSI’s stated specifications are based on the default settings. These selections are provided as part of the overall concept of the 2900 Series flexibility. To change the value of a Calibration parameter, simply touch the value to open the numeric keypad. Enter the new value, then touch [OK].
Page 75: Scheduler
Touch the [Set Auto-flush] button for a module to display the Auto-flush settings for that module. Touch the Interval (hours) button and enter the number of hours between Auto-flushes. Touch the Enabled [ON] button and change it to [OFF] to disable Auto-flush for this module. Touch [Save] to save your changes.
Page 76 The 2900 Series can be set to automatically calibrate at a specific time of day, such as the start of each workday. Touch the Scheduled calibration [OFF] button and change it to [ON]. Touch any days to select them. Days of the week that the scheduler is enabled are green.
Page 77: Cfr Part 11
FDA 21 CFR Part 11, Electronic Records; Electronic Signatures, was established by the FDA to define the requirements for submitting documentation in electronic form and the criteria for approved electronic signatures. Since YSI 2900 Series analyzers generate electronic records, these systems must facilitate compliance with 21 CFR Part 11.
Page 78 Touch the  to enable 21 CFR Part 11 mode. Enter a Name, User ID, and 8 character PIN to create at least one Admin account. Note that the PIN must be exactly 8 characters. Touch [Save].
Page 79 7.1.6.1 Manage Users Create User Touch the [Create User] button to create additional accounts as required. Leave the Admin box blank if you are creating a standard user. Touch the Admin box to mark it  ONLY if you are creating a new Administrator.
Page 80 Edit User To edit an existing user, select the user. Touch the [Edit User] button. Change the Name, PIN, Active, or Admin fields as required. To disable a user, remove the X from the Active box. After you have finished making your changes, touch [Save]. Touch the [X] button to return to the 21 CFR Part 11 screen. 7.1.6.2 View Audit Trail and Event Log Audit Trail Touch the [View History] button to display the audit trail.
Page 81 Use the scroll buttons to view events in the log. Export Plug a flash drive into the 2900 Series’ USB port, then touch the [Export] button to send the Audit Trail and Event Log to the flash drive. A folder named YSI\BiochemistryAnalyzer\21 CFR Part 11\ (followed by the Machine ID) will be created on the flash drive.
Page 82: Date/Time
7.1.7 Date/Time Date/Time To set the date/time, touch the time button on the main screen. NOTE: If 21 CFR Part 11 mode is enabled, only an Administrator can change the date/time. Hour Date Touch the current date on the calendar to select it. Touch the Hour button and enter the current hour in 24 hour format (0–23), then touch [OK].
Page 83: Service
Service Service Touch [Service] to display the Service menu. 7.2.1 Sipper See Section 4.5 Align Sipper for details on aligning the sipper with each sample module. If necessary, the sipper can also be aligned with Station 2, the different types of racks/plates used at Station 1, and the calibrator wells.
Page 84 Location To align the sipper at other locations, touch the button under Location. Touch the button for your location, [Station 2] for example. Touch the [Position] button and use the arrow buttons to align the sipper with the selected location.
Page 85 Touch  at the bottom right of the adjustment window, then touch [YES] to save the position. Touch [Inject] to lower the sipper and test the alignment, then touch [Retract] to raise the sipper back up. If necessary, touch [Position] and repeat the adjustment. 7.2.1.2 Depth Touch [Depth] and select the location.
Page 86: Pumps
Touch  at the bottom right of the adjustment window, then touch  to confirm saving the position. 7.2.2 Pumps From the Service menu, touch the [Pumps] tab. 7.2.2.1 Sipper Pump Home Touch the [Home] button to Home the sipper pump plunger. The pump plunger will extend fully, then retract slightly. Aspirate Touch the [Aspirate] button.
Page 87: Modules
7.2.3.2 Calibrate The 2900 Series will automatically calibrate before running a sample batch. You may initiate manual calibration from the [Modules] tab of the Service screen by touching the [Calibrate] button under each Module.
Page 88: Stirbar
The instrument attempts to calibrate each active probe up to 5 times before aborting calibration. If calibration fails, see 11 Troubleshooting. 7.2.4 Stirbar From the Service menu, touch the [Stirbar] tab. NOTE: If 21 CFR Part 11 mode is enabled, only an Administrator can access the Stirbar tab.
Page 89 The StirbBar screen is used to adjust the speed of the stir bar. The stir bar has two operating speeds: stir speed, where the stir bar rotates smoothly in the sample module and jump speed, where the stir bar jumps to help clear air bubbles from the module during the flush cycle.
Page 90: Monitor
7.2.5 Monitor From the Service menu, touch the [Monitor] tab. Test Outputs Touch the DAC A or DAC B [0.00V] button to test the analog output by setting the output to 0, 5 or 10VDC. Touch the Ready, Feed Pump, Filtrate Pump, or SysErr [Off] button and change it to [On] to test the corresponding output. Valve Touch the Opened Valve [Antiseptic] button to change the state of the valve and change it to [Sample] Prime Monitor Pump...
Page 91: Data
Data From the Main display, touch [Data]. 7.3.1 Plate Historical plates of sample data are displayed under the Pate tab. Touch the scroll buttons to page through plates. Touch a specific plate to highlight it, then touch [View] to display the sample data for the selected plate. Sample data for the first sample in the batch (A1) is displayed.
Page 92 Select sample Select to show details Touch [Close] to return to the Data screen. Plate Name Filter Touch the Name button and enter a plate name to filter the data. Only plates that begin with that name will be displayed. Export Check the To Export box for each batch that you would like to export to a flash drive.
Page 93: Monitor
Plug a flash drive into the 2900 Series’ USB port, then touch the [Export] button to send the selected sample results from memory to the flash drive. When you have finished exporting data, touch the [X] button to return to the Main screen.
Page 94 Touch [View Data Points] to view the actual data. Touch [Close] to return to the Data screen. Name Filter Touch the Name button and enter a monitor session name to filter the data. Only sessions that begin with that name will be displayed. Export Check the To Export box for each session that you would like to export to a flash drive.
Page 95: Calibration
7.3.3 Calibration Touch the Calibration tab. Plug a flash drive into the 2900 Series’ USB port. Select a date range, then press the [Export] button to send calibration data to the flash drive. When you have finished exporting data, touch the [X] button to return to the Main screen.
Page 96: Help
NOTE: The Machine ID is used as the folder name and file name for data files. The 2900 Series software can be updated via a flash drive inserted into the USB port. See the YSI web site at www.ysi.com for available updates. Install the update as shown below.
Page 97: Software
7.4.2 Software From the Help menu, touch the [Software] tab to display the Software screen. NOTE: If 21 CFR Part 11 mode is enabled, only an Administrator can access the Software tab. 7.4.2.1 Export Configuration Insert a flash drive and wait for the Flash Drive Inserted light to change to green. Press [Export Config] to save the current instrument configuration to the flash drive.
Page 98: Faq
From the Help screen, Software tab, insert the flash drive containing the software update in the instruments’ USB port. After the Flash Drive Inserted light changes to green, touch the [Launch Updater] button. The 2900 Series Updater will be displayed. Touch the [Update Firmware] button to install the new firmware.
Page 99: Ethernet Port
While the video is playing, you may touch the screen to stop the video and return to the Training screen. Ethernet Port Connect one or more 2900 Series instruments to a LAN or router via the RJ45 Ethernet port. 7.5.1 LAN (Shared Network Connection) Connect one or more 2900 Series instruments to a LAN via the RJ45 Ethernet port.
Page 100: Accessing Stored Data
7.5.3 Accessing Stored Data Access the data stored in a 2900 Series connected to a LAN or router via ftp. See 7.4.1 About to view the instrument’s current IP address. If you are unable to connect via ftp, make sure the “Passive ftp” option is not checked under Advanced Internet Options...
Page 101 Sample and calibration data files are stored in the Samples folder of the 2900 Series. The file names are: BioSample_Machine ID.csv BioCalibration_Machine ID.csv ISESample_Machine ID.csv ISECalibration_Machine ID.csv...
Page 102: Chemistry Setup
8. Chemistry Setup In this section, you will learn about each standard chemistry setup for single chemistry configurations and then dual chemistry configurations per module. In order to configure your instrument to measure a particular chemistry analyte, you need to: •...
Page 103: Choline
Detection 5–450 mg/L Range For example, to express results as a choline hydroxide value, program the calibrator value as 204 mg/L when using the YSI Calibration 175 mg/L 2772 Choline Standard calibrator solution. Your results will now Point reflect the concentration of choline hydroxide in the sample.
Page 104: Ethanol/Ethanol-Hc
• The linear range of ethanol is quite limited. If you are System concerned about linearity, monitor the upper range of YSI 1579 YSI 2787 Buffer concentration on a regular basis. You may benefit by preparing and using a calibrator with an ethanol Calibrator concentration close to your sample concentration.
Page 105: Galactose
25 µL Sample Size End Point 30 sec • YSI believes that you will be able to measure this analyte Typical Working ~10 days for many applications in the range specified. However, Life YSI makes no claims with respect to precision or linearity.
Page 106: D-Glucose (Dextrose)
(2.50g/L Cal Point) • For applications requiring linearity performance to Calibration 25.0 g/L, YSI 2777 (25.0 g/L glucose, 2.50 g/L lactate) 2.50 g/L Point may be used as a linearity standard provided the calibrator is YSI 2776 (2.50 g/L glucose, 0.50 g/L Linearity Check 9.0 g/L...
Page 107: L-Glutamate (L-Glutamic Acid)
To express results as an MSG value, change the glutamate units Point to a w/v expression, and enter one of the following: 936 mg/L, 0.936 g/L, or 0.094 %. Use YSI 2755 Calibrator Standard (5.00 Linearity Check 1462 mg/L mmol/L Glutamate) to calibrate the instrument. Sample results Point will then reflect MSG concentration.
Page 108: L-Glutamine
8.2.6 L-Glutamine This is a direct reading of glutamine in solution at the enzyme sensor. Two enzymes are co-immobilized in the YSI Glutamine Membrane: Glutaminase and Glutamate Oxidase. Through this chain of reactions the amount of hydrogen peroxide liberated is directly proportional to the amount of glutamine.
Page 109: Glycerol
40.0 g/L Point • Always use fresh YSI 2705 Buffer in an opaque buffer bottle, such as the YSI 2935, when installing a new YSI 10 µL Sample Size 7140 Glycerol Membrane. Do not expose the 2705 Buffer End Point...
Page 110: Hydrogen Peroxide
This membrane contains immobilized nonenzymatic protein to produce diffusion properties similar to those exhibited by YSI Enzyme Membranes. The electrochemical reaction, which is common to all YSI Enzyme Sensors, is the following: + 2e...
Page 111: L-Lactate
YSI 1530 • D-Lactate is not a substrate for L-Lactate Oxidase. Membrane YSI 2329 Therefore, the 2900 Series cannot directly measure D-Lactate. If you have a known racemic mixture of Membrane Gray lactates, the L-Lactate value multiplied by 2 should give Color you the total lactate value.
Page 112: Lactose
Detection 0.05–25 g/L Range • The enzyme membrane integrity test involving the use of Calibration 5.0 g/L YSI 2363 Potassium Ferrocyanide solution is not Point informative since ferrocyanide exists in the 2705 system buffer. Linearity Check 25.0 g/L Point 25 µL...
Page 113: Methanol
Methanol + O + Formaldehyde Alcohol Oxidase Special Considerations: System Buffer YSI 1579 • Calibrator Std The linear range of methanol is quite limited. If you are YSI 2726 concerned about linearity, monitor the upper range of Solution A concentration on a regular basis.
Page 114: Sucrose
8.2.12 Sucrose This is a direct reading of Sucrose in solution at the enzyme sensor. Three enzymes are co-immobilized in the YSI Sucrose Membrane: Invertase, Mutarotase, and Glucose Oxidase. Through this chain of reactions the moles of hydrogen peroxide liberated is directly proportional to the moles of sucrose.
Page 115: Xylose
D-Xylose + O + D-xylosone Pyranose Oxidase Special Considerations: System Buffer YSI 2357 • If sample dilution is required, use reagent water or YSI Calibrator Std YSI 2767 buffer. Linearity Std YSI 2768 • The sample must be glucose-free, or at least contain...
Page 116: Simultaneous Ammonium And Potassium
The interference is System relatively small on a weight/weight basis (<1%). The YSI 2970 Buffer 2900 Series does not automatically compensate for the presence of sodium. In order to check the potential Calibrator YSI 2972 YSI 2971...
Page 117: Simultaneous Glucose And L-Lactate
Refer to the sections above on Glucose and L-Lactate for theoretical and special considerations. Then follow the instructions below to set up your instrument for simultaneous determination. A Probe B Probe Chemistry Glucose L-Lactate System Buffer YSI 2357 Calibrator Std YSI 2776 Linearity Std YSI 1531 YSI 1530 Membrane YSI 2365 YSI 2329 25 µL...
Page 118: Simultaneous Glucose And Sucrose
Contact YSI Technical Support or your dealer representative for an application note explaining this approach in detail.
Page 119: Simultaneous Glucose And Xylose
As with most analytical methods there is a concentration Sample Size range that provides optimal accuracy and precision. The Unit of YSI xylose and glucose methods are specified Conc. independently for performance; however these two analytes frequently occur together. Cal Value 2.50...
Page 120: Simultaneous L-Glutamate And L-Glutamine
Glutamine Glutamate enzyme reactions and the final reaction is a “glutamate System YSI 2357 measurement”, free glutamate in a sample will be measured at Buffer the Glutamine Sensor. By using the information at the Glutamate Sensor, this free glutamate is automatically subtracted in the...
Page 121: Daily Maintenance
If the 2900 Series is used for medical research or biological testing, dispose of the waste bottle contents in a manner suitable for biohazardous waste. The YSI reagents used in the 2900 Series are non-toxic and, unless otherwise specified, consist of a phosphate salt buffer with small amounts of preservatives.
Page 122: Preventive Maintenance - 6 Months Or 1000 Hours
Repeat this entire procedure for any additional calibrator bottle positions that you use. Preventive Maintenance – 6 months or 1000 Hours Before performing maintenance on the 2900 Series, ALWAYS turn the instrument off and unplug the power cord from the wall outlet.
Page 123: Waste Module Cleaning
Unscrew and remove the enzyme/ISE probes and the temperature probe from each sample module. Place the ISEs and reference electrodes in 2970 Buffer solution to prevent them from drying out. Reference Electrode Temperature Probe Ammonium ISE Potassium ISE Figure 9.2 Remove the sample modules.
Page 124: Ise Cleaning
1. Soak the ISE with distilled or deionized water for 30 minutes. 2. Recondition the ISE by soaking it in YSI 2970 buffer solution for 4 hours. 9.4.5 Sipper pump Seal Replacement Replace the Sipper Pump seals every 6 months.
Page 125 Sipper pump head Figure 9.4 Pull the white pump base from the clear pump housing. If salt deposits have formed on the parts, wash them off with warm water. Make sure the metal pipes where the tubing connects are not blocked or restricted. Replace the seals as shown in Figure 9.5.
Page 126: Bottle Tubing
WARNING: When re-installing the pump head assembly, the plunger MUST extend at least 1/2″ from the base of the pump (see Figure 9.6). This will assure proper alignment between the pump head and the drive hub. 9.4.6 Bottle Tubing Disconnect the tubing and unscrew the cable connectors from the buffer, waste and calibrator bottles on each side of the instrument.
Page 127 Sipper Pump Buffer Pump 1 BLUE C1B Pump C1A Pump Pumps – Right side Figure 9.8 C2A Pump Buffer Pump 2 BLUE C2B Pump Buffer Pump 3 BLUE C3B Pump C3A Pump Pumps – Left side Figure 9.9...
Page 128: Pump Tubing Replacement
9.4.7 Pump Tubing Replacement Tubing life depends on instrument usage. The buffer and calibrator pump tubing should be replaced at least every 6 months or 1000 hours sample ready. NOTE: The buffer pump tubing and calibrator pump tubing each require a different type of grease. It is important to apply the correct grease to each type of tubing.
Page 129 Figure 9.11 Remove the pump tubing from each pump. Install Buffer Tubing Apply plenty of buffer grease (included with the new buffer pump tubing) to the new buffer pump tubing. Apply the grease to the large diameter section of each tubing. Do not apply calibrator pump grease to the buffer pump tubing. Insert the new pump tubing around the pump roller assembly and into each buffer pump.
Page 130 Apply plenty of grease (included with the new pump tubing) to the longer section of new calibrator pump tubing. Insert the new pump tubing around the pump roller assembly and into calibrator pump C2A. Make sure the short section of tubing is on the left side of the pump. The tubing attached this fitting connects to the left fitting on waste module 2. Place a red pump head cover onto the cal pump and press until it snaps into place.
Page 131: Install Waste Modules
Apply plenty of grease (included with the new pump tubing) to the longer section of new calibrator pump tubing. Insert the new pump tubing around the pump roller assembly and into calibrator pump C3A. Make sure the short section of tubing is on the left side of the pump. The tubing attached this fitting connects to the left fitting on waste module 3. Place a red pump head cover onto the cal pump and press until it snaps into place.
Page 132: Install Sample Modules
Connect waste tubing 2 to fittings Connect waste tubing 3 to fittings Calibrator and Waste Tubing (Modules 2 and 3) Figure 9.14 9.4.10 Install Sample Modules After cleaning, flush the sample modules with copious amounts of warm water, then rinse with distilled water to remove any traces of the disinfecting agent.
Page 133: Calibrate Sipper
Unscrew the sipper cone, slide it down and remove it from the sipper. Loosen the two needle mount screws two turns, then remove the sipper by sliding it straight out of the mounts. Carefully insert the long thin end of the new sipper and slide it into the mounts. Slide the sipper cone up the sipper and screw it into the sipper arm.
Page 134: Fuse Replacement
Fuse Replacement It may be necessary to replace the fuse in the back of the 2900 Series. New fuses may be purchased from YSI or obtained from many local electrical component suppliers. Be sure to obtain the correct fuse rating as indicated below.
Page 135 halves of the pump body. Then remove the roller assembly, noting the slot in one end of the center shaft of the assembly. This slot must engage with the pump motor shaft on the 2960. Be certain to orient the "slot end" correctly on reassembly. Remove the old tubing, removing tubing retainers as required.
Page 136: Monitor Sample Cup
Repower the instrument, if necessary. From the Service menu, Monitor tab, check pump function for proper flow direction. Also listen for any unusual sounds that suggest strain on the pump motor. This may indicate binding or twisting of the newly installed tubing. 9.6.1.2 Solenoid Valve Tubing Replacement Before changing solenoid valve tubing first study the tubing placement carefully.
Page 137: Storage
Instrument Storage If the 2900 Series is not going to be used for 2 weeks or longer, remove the buffer and calibrator solutions from the bottles and replace them with deionized or distilled water. Flush the water through the system thoroughly, empty the bottles, reinstall them in the instrument and prime the system with air.
Page 138 • Review Section 7.2, to learn more about how you can test individual components of the 2900 Series. • Use the troubleshooting chart in this section to assist you in identifying the problem, then use the chart to guide you to a corrective action.
Page 139: Printout Information
25.9 ----------------------- ----------------------- Volume (uL) 1B:Lactate 0.50 g/L Dilution Factor IB nA 0.88 Fri 9/9/2015 10:59:37 NPL nA 9.81 YSI 2950 – 15F000025 FB nA 0.76 ======================= PL Slope nA/m 0.42 IB Shift -1.95% NPL Shift 1.64% ======================= Temp (C) 25.9...
Page 140 The default setting is 2%. That is, if the shift is greater than 2%, the instrument performs another calibration. Note that the 2900 Series attempts to calibrate each sensor up to 5 times before aborting calibration for that sensor. You may select Cal shift values that better suit your application.
Page 141: Ion Selective Electrodes
IB(mV): 28.63 27.02 NPL(mV): 6.09 37.42 Avg: 6.12 37.43 Std Dv: 0.17 0.02 End Point 30 sec Sample size 25 uL Temperature 21.50 C --------------- ->Coef: 0.19 0.02 ->Slp: 57.18 60.05 --------------- Tue 10/25/2015 11:08:32 YSI 2950 – 13J000024 ==========================...
Page 142 ======================== Temp (C) 25.33 Sample Results Report Volume (uL) ======================== Dilution Factor Batch: TestBatch-1 Wed 10/04/2015 1:31:09 Analyte: P96_B02 YSI 2950D – 15J000024 ------------------------ ======================== 3B: Potassium 193 mg/L Wed 10/04/2015 1:31:09 ======================== ======================== Sample Results Report ======================== Batch: P96-1...
Page 143 10° and 50°C. As the 2900 Series is calibrating the ISEs, it prints out information for each sensor as shown below. Calibrations are repeated until the standard deviation of both sensors is less than 0.20 (or the Change Ratio is less than or equal to 1.2%) for each calibration solution.
Page 144: Troubleshooting Chart
11.2 Troubleshooting Chart SYMPTOM: MEASUREMENT ERROR: IB Level Error POSSIBLE CAUSE: Pinched, leaking or disconnected tube. ACTION: Fix or replace tubing. SECTION: 9.4.7 Pump Tubing Replacement POSSIBLE CAUSE: Sipper misaligned. ACTION: Check Sipper alignment. SECTION: 4.5 Align Sipper POSSIBLE CAUSE: Sipper pump not operating properly.
Page 145 POSSIBLE CAUSE: Newly installed enzyme Membrane. ACTION: Enter probe service and check probe currents. SECTION: 7.2.3 Modules POSSIBLE CAUSE: Newly installed probe. ACTION: Enter probe service and check probe currents. SECTION: 7.2.3 Modules POSSIBLE CAUSE: Calibrator solution out of spec: contaminated or installed for more than 30 days.
Page 146 ACTION: Empty waste bottle. SECTION: 9.2.1 Empty the Waste Bottle POSSIBLE CAUSE: Bubbles in buffer or calibrator bottle tubing. ACTION: Prime bottle. SECTION: 4.10 Prime the Fluid System SYMPTOM: INTERNAL FAILURE: Unable to Calibrate POSSIBLE CAUSE: Air bubbles in calibrator tubing. ACTION: Prime calibrator bottles.
Page 147 SECTION: 4.5 Align Sipper POSSIBLE CAUSE: Calibrator bottle not primed. ACTION: Prime calibrator bottle. SECTION: 4.10 Prime the Fluid System POSSIBLE CAUSE: Low calibrator solution. ACTION: Install new calibrator. SECTION: 4.8 Install Calibrator Solution(s) POSSIBLE CAUSE: Pinched, blocked, leaking or disconnected tube. ACTION: Fix or install new tubing.
Page 148: 2960 Online Monitor
SECTION: 9.6.1 Tubing Replacement, Pump Tubing Replacement SYMPTOM: Monitor sample results appear to be lower than expected and excess air is observed in the 2900 Series sample chamber. POSSIBLE CAUSE: Sipper depth is not set properly at Monitor Cup. ACTION: Set sipper depth at Sintini Cup.
Page 149 SYMPTOM: Sipper aspirates antiseptic instead of sample (sample results read approximately zero) POSSIBLE CAUSE: Tubing routed incorrectly in 2960 solenoid valve. ACTION: Check 2960 valve tubing installation. SECTION: 9.6.1Tubing Replacement, Solenoid Valve Tubing Replacement...
Page 150: Principles Of Operation
12.1 Enzyme Sensor Technology The enzyme sensor technology of the 2900 Series is based on the principles conceived by Dr. Leland Clark, formerly of Children’s Hospital Foundation, Cincinnati, Ohio. The immobilized enzyme membrane was invented by YSI and is covered by U.S. Patent 4,073,713. This sensor technology has been used successfully since 1975.
Page 151: Ion Selective Electrode
12.3 Measurement Methodology The 2900 Series employs a steady state measurement methodology. A typical enzyme sensor response is shown in Error! Reference source not found.. Typical Enzyme Sensor Response Figure 12.2 When sample or calibration standard is dispensed into the sample module, it is diluted into 600 microliters of buffer.
Page 152: Baseline Stability
The sensors’ net value for a calibration (PL) is displayed and printed. An unstable calibration is displayed and printed as a "PL shift". While establishing a stable calibration, the 2900 Series will run 5 calibrations before aborting calibration for a sensor. The flexible parameter selection allows the user to disable this error mode.
Page 153: Temperature Compensation
13. Warranty and Repair YSI 2900 Series Biochemistry Analyzers are warranted for one year from date of purchase by the end user against defects in materials and workmanship, exclusive of batteries. Within the warranty period, YSI will repair or replace, at its sole discretion, free of charge, any product that YSI determines to be covered by this warranty.
Page 154: Cleaning Instructions
Disposition costs will be the responsibility of the sender. When service is required, either at the user's facility or at YSI, the following steps must be taken to insure the safety of our service personnel.
Page 155: Ysi Factory Authorized Service Centers
13.2 YSI Factory Authorized Service Centers United States Europe YSI Incorporated YSI Life Sciences Repair Center Xylem 1725 Brannum Lane Longfield Road Yellow Springs, OH 45387 Tunbridge Wells Phone: 937 767-7241 Kent TN2 3EY Fax: 937 767-9353 Phone: 44 1892 500400...
Page 156: Notices
YSI Incorporated declares that the instrument specified above conforms to the essential requirements of the Directives and Standards specified above when installed and operated in accordance with specifications as set forth by YSI. This product has been tested to the requirements of CAN/CSA-C22.2 No. 61010-1, third edition, or a later version of the same standard, incorporating the same level of testing requirements.
Page 157: Declaration Of Conformity - Australian And New Zealand Electromagnetic Compatibility
Australian and New Zealand Electromagnetic Compatibility Manufacturer: YSI Incorporated 1725 Brannum Lane Yellow Springs, OH 45387 USA Product Name: YSI Model 2900 Series Biochemistry Analyzer Model Number(s): YSI 2900D, YSI 2950D-x Conforms to the following: Standards: Australian and New Zealand AS/NZS 61000.3.2:2007+A1:2009...
Page 158: Radio And Television Interference Notice
14.3 Radio and Television Interference Notice This equipment generates and uses radio frequency energy and if not installed and used properly, may cause interference to radio and television reception. There is no guarantee that interference will not occur in a particular installation. If this equipment does cause interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures: •...
Page 159: Appendix A - Software Flowchart
15. Appendix A – Software Flowchart The software flow chart for the 2900 Series is shown below in Figure 15-1. The main screen has six icons that control all instrument functions (shown at the top of the flowchart). Main Menu...
Page 160: Appendix B - Concentration Unit Conversion
16. Appendix B – Concentration Unit Conversion In the 2900 Series Configure menu, you have the option to assign units of concentration. There are default values set based on calibration solutions offered by YSI. Below is a table of unit conversions for these calibration solutions and other concentrations of interest.
Page 161: Linearity Test. Concentration Unit Conversion
16.1 Linearity Test. Concentration Unit Conversion mg/L Chemistry (ppm) mg/dL (w/v) mmol/L (g/mole) 0.47 0.047 4.54 Choline 0.45 0.045 4.32 (104) 0.43 0.043 4.10 9.45 9,450 0.945 52.5 Glucose (Dextrose) 9.00 9,000 0.900 50.0 (180) 8.55 8,550 0.855 47.5 3.36 3,360 0.34 73.1...
Page 162: Fcn Membrane Integrity Test. Concentration Unit Conversion
0.33 (150) NOTE: Use the values from the preceding tables only when calibrating with the appropriate YSI calibrator solution Choline (2772), Glucose (2776 or 2747), Ethanol (2790), Ethanol-HC (7151), Sucrose (2780), L-Glutamate (2755), L-Glutamine (2736), Methanol (2726), Xylose (2767) and L-Lactate (2776).
Page 163: Appendix C - Line Power Cord And Plug Wiring
17. Appendix C - Line Power Cord and Plug Wiring United Kingdom United States Europe Figure 17.17-1...
Page 164: Appendix D - Reagents And Accessories
18. Appendix D - Reagents and Accessories YSI Number Description Comments 1579 Carbonate Buffer For use with Ethanol or Methanol membranes 2357 Buffer Kit (8 packs of Buffer Concentrate) For use with Glucose, L-Lactate, Sucrose, L- Glutamate and L-Glutamine membranes.
Page 165 R8 12mm Test Tube Rack 2948 R4 16mm Test Tube Rack 2960 Online Monitor & Control Accessory 2988 Preventive Maintenance Kit, 2900 Tubing, Sipper, and O-rings for 6 month maintenance 2990 Preventive Maintenance Kit, 2960 Tubing and O-rings for 6 month maintenance of...
Page 166 1725 Brannum Lane Yellow Springs, Ohio 45387 USA 937 767-2769 800 659-8895 Fax 937 767-8058 527626 Support@ysi.com A527626H www.ysi.com 2016-06 2016 Xylem Inc. YSI is a trademark of Xylem Inc. or one of its subsidiaries.

YSI 2900 Operation And Maintenance Manual

1 Introduction

2 Safety

3 Getting Started

4 Basic Setup

5 Running the Instrument

6 Online Monitor and Control

7 System Configuration

8 Chemistry Setup

9 Operational Checks and Maintenance

10 Storage

11 Troubleshooting

12 Principles of Operation

13 Warranty and Repair

14 Notices

15 Appendix A - Software Flowchart

16 Appendix B - Concentration Unit Conversion

17 Appendix C - Line Power Cord and Plug Wiring

18 Appendix D - Reagents and Accessories

Quick Links

Troubleshooting

Need help?

Questions and answers

Related Manuals for YSI 2900

Summary of Contents for YSI 2900

Table of Contents