Shodex Asahipak NH2P-50 10E Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)

Important Handling Instructions

Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.

Before Using the Column

(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.

1. Introduction

Thank you for purchasing the Shodex product. Shodex Asahipak NH2P series is polymer-based
chromatography columns modified with amino group. The column is suitable for the analysis that requires
hydrophilic chromatography (HILIC) or ion exchange chlromatography .

2. Column Components

Please refer to the Shodex website: https://www.shodex.com/en/da/07.html

3. Column Specifications

Product
Product Name
Code
F6830001 Asahipak NH2P-50 10E
F6710016 Asahipak NH2P-50G 4A
Base Material : Spherical porous particles of polyvinyl alcohol modified with amino group
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Acetonitrile

4. Usable Conditions

Product Name
Asahipak NH2P-50 10E
Asahipak NH2P-50G 4A
Usable solvents are listed below.
(1) A mixture of water and acetonitrile or water and ethanol of any ratio can be used. Up to 100 % water,
acetonitrile, and ethanol can be used. Use of organic solvents other than acetonitrile or ethanol is not
warrantied.
Operation Manual
Shodex Asahipak NH2P-50 10E
Column Size (mm)
I.D.
10.0
4.6
Flow Rate (mL/min)
Recommended Maximum
2 - 3
-
Resonac Corporation (https://www.shodex.com/)
Particle Size
(μm)
Length
250 5
10 5
Maximum
Pressure
(MPa/column)
5 15
- -
ver.20221202E
Theoretical Plate Number
(Per Column)
≥ 10,000
(Guard Column)
pH Temperature
Range Range (°C)
2 - 13 4 - 50
1