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6 OPERATION
Colorimetry is a relative method, therefore an initial calibration is needed before measurement
can be performed. This is done using standard solutions and analyzing them in the same way as
the sample.
In order to ensure correct measurement performances, the analyzer should be calibrated
periodically. The blank is taken with DI water. The system slope is controlled by a comparison to
a standard solution of known concentration.
Calibration allows the analyzer to adjust:
Blank of the system
Slope of the system (factor calibration)
The calibration can be done manually or automatically.
REAGENTS PRIME
6.1
Prior to the initial calibration of the analyzer, the reagent lines must be primed. This procedure
should also be performed every time the reagents are refilled or replaced.
1. Login with the 1st level service password (1111).
2. Press the DISPLAY button on the touchscreen and then press the button MANUAL
STEP.
3. Select SAMPLE function for 20 seconds.
4. Select ADD REAG (1,2) functions for 40 seconds each in order to prime the three
reagent lines. Visually confirm that reagent is being drawn into the lines and into the
flowcell. If no reagent is seen, it may be necessary to repeat the ADD REAG function.
5. Select DRAIN function for 5 seconds.
6. Select SAMPLE function for 20 seconds.
7. Repeat steps 5 and 6 to rinse the flowcell.
8. Check the drain tube of the pinch valve is correctly positioned and select the DRAIN
function for 5 seconds.
BLANK CALIBRATION
6.2
A blank calibration should be performed after reagent replacement and every time a manual
calibration is required.
1. Login with the 1st level service password (1111).
2. Disconnect the sample tubing from the fast-loop reservoir and connect it to a DI water
source.
3. Perform an analysis cycle by pressing the RUN Menu and selecting CH 1.
It is recommended to proceed with a blank refreshment after performing three
analysis cycles with DI water to flush the system.
The absorbance detected by the analyzer is assumed to be absorbance produced by
reagent(s) and DI water.
4. To store this value as the blank, from the PROGRAM menu, select the CALIBRATION
Waltron User Manual
3051 Hardness Analyzer
Operation
40
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