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® HG-400 MiniG HG-400 MiniG® Tissue and Cell Lyser for Biological Applications Operation Manual For 115V/230V (04500-08) Cole-Parmer® Page 1 of 20 1300-87041-1M, REV 5...
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Metuchen, NJ 08840. SPEX SamplePrep is now part of Cole-Parmer®. The Cole-Parmer® HG-400 MiniG was formerly known as SPEX 1600 MiniG. Over the years, we’ve acquired many high-quality and reputable brands. After many years of continual growth, we realize our brands are all as brilliant as each other. Rather than have a portfolio of complementary brands, we felt consolidating them under one world- class brand name enabled us to offer a single and significant brand experience.
® HG-400 MiniG 1.0 INTRODUCTION The HG-400 MiniG® is a mechanical disrupter that is designed to effectively disrupt cellular materials by oscillating one or two deep-well titer plates vertically. This motion allows the unit to be used to prepare sample tissue for extractions of nucleic acid, protein, and other constituents by shaking the tissue, steel balls and a buffering agent together in each well of the titer plate.
Capacity: Maximum sample load 1.2 lb. (0.54 kg) NOTE: Operating with loads that exceed the recommended maximum weight limits can result in damage to the HG-400 MiniG®. Therefore, warranty restrictions or invalidation may apply. Typical Load: 1 or 2 titer plates (stacked)
® HG-400 MiniG 3.0 UNPACKING Carefully inspect the exterior of the packing box. Any visible damage should immediately be reported to the carrier. Remove all packing documents from the exterior of the box and file in your records. Remove the top of the shipping box, the foam packaging material, and the HG-400 MiniG®...
To install the clamp, use the 3/16 in. Allen wrench (supplied in bag with power cords and operating manual) and the three bolts and lock washers (used to secure shipping block to shaft) supplied with the HG-400 MiniG®. Open the MiniG®...
Note: To avoid injury, follow the safety information in this section. Keep these instructions for future reference. Caution: Every effort has been made to ensure the HG-400 MiniG operates at a moderate noise level. The intensity of noise is directly impacted by the runtime, rate, container type, as well as the amount of balls.
HG-400 MiniG 6.0 CONTROLS The control panel is located on the front of the HG-400 MiniG® (Figure 2). Upon start-up, “COLE-PARMER” and “HG-400 MiniG” will appear briefly on the display screen followed by the run time. Display Increase (Time/Rate) Decrease...
® HG-400 MiniG 6.2 Rate Control To adjust or display the operating rate press the Time/Rate button located below the display. The display will then display the operating rate. Then use the plus and minus buttons to increase or decrease the operating rate in increments of 25 rpm. The minimum rate is 500 rpm;...
HG-400 MiniG 7.0 CLAMP The clamp is the most critical component of the HG-400 MiniG®, as it must be carefully adjusted to hold the titer plates or vials firmly in place. The up-and-down motion of the clamp is extremely vigorous. Sample holders must be held securely in the clamp during operation to prevent damage and leakage.
The sample load must be evenly distributed across the clamp to avoid excess wear to the HG-400 MiniG®. Also, the clamp lid must have an even surface upon which to rest. If running less than a full holder of vials, be sure to place vials at the 4 corners and 2 in the center (Figure 4) to support the clamp lid.
WARNING: Due to the weight of the cryo accessories, only one cryo-block or one cryo- adapter may be run. Do not attempt to stack cryo-blocks or cryo-adapters!! Excess weight can cause severe damage to the HG-400 MiniG®. 7.4 Releasing the Clamp 1.
® HG-400 MiniG 8.0 OPERATION Before operating the HG-400 MiniG®, it is advisable to perform several “dry runs” to become familiar with the clamp and controls. The following steps outline a standard operating cycle: 1. Load and cap the titer plates or vials.
For example, do not use glass vials in the HG-400 MiniG®. Impact of the grinding media may cause the vials to break, resulting in broken glass and spilled samples.
® HG-400 MiniG 11.0 MAINTENANCE The HG-400 MiniG® has been designed to provide trouble-free operation over a long period of time. To assure proper performance, the most important factor is cleanliness. Any spills should be wiped up immediately. The HG-400 MiniG®...
® HG-400 MiniG 12.0 PARTS LIST Item Part Number Description 1690 Complete Clamp Assembly 1690T Nesting Plate (sold separately) 1691 Clamp Lid 51209 Locking Lever (2 per Lid required) Figure 6 93850 Fuse, 8 amp, 250V slow-blow, (2 per unit required) Cole-Parmer®...
® HG-400 MiniG 13.0 WARRANTY Cole-Parmer® guarantees its products against defects in materials or workmanship for three years from the date of original shipment. Repairs, replacements, or parts are guaranteed for 30 days or for the remaining original warranty period (whichever is greater) for the item that was repaired or replaced.
® HG-400 MiniG 14.0 INSTRUMENT DISPOSAL In accordance to the EU Directive 2012/19/EU covering Waste Electrical and Electronic Equipment, all equipment with the disposal symbol must not be disposed of with general waste. (See Figure 7) Disposal Label is located on the back of unit.
® HG-400 MiniG 15.0 CONTACT US Repair Service Phone: 1.732.623.0465 Cole-Parmer 65 Liberty St Metuchen, NJ 08840 Attn: Service and Repair Please include RA Number on the shipping label. Germany T: +1.800.323.4340 or T: +49 (0) 9377 92030 +1.800.323.4340 E: de.sales@antylia.com E: sales@antylia.com...
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