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Summary of Contents for Cole Parmer Freezer/Mill CG-400
- Page 1 CG-400/CG-450 Freezer/Mill® Cryogenic Grinder Designed for Milling Tough and Temperature Sensitive Samples Operation Manual For 115V (04500-40,04500-41) and 230V (04577-92, 04577-93) 1300-87051-1M, REV 6...
- Page 2 CG-400/CG-450 Freezer/Mill Copyright 2023 by Cole-Parmer® All rights reserved. No part of the work may be reproduced or transmitted in any form or by any means, electronic or mechanical; including photocopying and recording, except as may be expressly permitted by the 1976 Copyright Act or in writing from the publisher. Requests for permission should be addressed in writing to Cole-Parmer®, 65 Liberty Street, Metuchen, NJ 08840.
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Page 3: Table Of Contents
CG-400/CG-450 Freezer/Mill TABLE OF CONTENTS SECTION DESCRIPTION PAGE Introduction ........................5 Specifications ........................6 Unpacking ........................... 7 Setting Up ..........................8 Electrical Connection ......................9 Power Switch and Controller ....................9 Lid, Coil Assembly, and LN Sensor ..................10 Grinding Vial Sizes, Adapters, and Vial Openers………………………………………………………….11 Touchscreen Display ...................... - Page 4 CG-400/CG-450 Freezer/Mill TABLE OF CONTENTS SECTION DESCRIPTION PAGE 12.0 Warranty ........................... 31 12.1 Product Specifications ...................... 31 12.2 To Arrange a Return Shipment ..................31 13.0 Instrument Disposal ......................32 14.0 Contact Us ......................... 33 Cole-Parmer® Page 4 of 33 1300-87051-1M, REV 6...
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Page 5: Introduction
CG-400/CG-450 Freezer/Mill 1.0 INTRODUCTION Cole-Parmer Freezer/Mills are laboratory mills that cool unconventional materials to cryogenic temperatures and pulverize them to a powder form without thermal degradation. Our proven technology uses a dual electromagnetic grinding chamber that rapidly drives a steel impactor back and forth against the two end plugs of the sample vial. -
Page 6: Specifications
CG-400/CG-450 Freezer/Mill 2.0 CG-400/CG-450 FREEZER/MILL SPECIFICATIONS Type of Mill: Cryogenic impact mill Controller: Touchscreen Data Transfer: Dual USB Port (back of controller) Grinding Mechanism: Steel impactor driven by dual electromagnets Coolant: Liquid nitrogen (LN) Dimensions: 20½ in. (52 cm) x 21½ in. (55 cm) x 18 in. (46 cm) Weight: (empty, without vial or coolant) approx. -
Page 7: Unpacking
CG-400/CG-450 Freezer/Mill 3.0 UNPACKING Inspect the exterior of the packing box and report any visible damage to the carrier. Remove all packing documents from the exterior of the box, and save them for your records. Open the top of the shipping box. Remove the packing material and accessories, and gently remove the CG- 400/CG-450 Freezer/Mill. -
Page 8: Setting Up
CG-400/CG-450 Freezer/Mill 3.0 UNPACKING (Cont’d) TOUCHSCREEN CONTROLLER [Dual USB PORT BACK OF CONTROLLER] Figure 2 – CG-400 Freezer/Mill, Rear View 4.0 SETTING UP The CG-400/CG-450 Freezer/Mill weighs 44 pounds (20 Kg) empty. The lid opens from the front and the lid latch is in the center. The AC Input module and the ON/OFF switch (which also serves as a circuit breaker) are on the back of the cabinet. -
Page 9: Electrical Connection
CG-400/CG-450 Freezer/Mill 4.1 Electrical Connection Plug the electrical cord into the inlet on the back of the Freezer/Mill cabinet, and then into a standard 3-prong grounded electrical outlet. The 230V/50HZ, CG-400/CG-450 Freezer/Mill is supplied with a standard European 2-prong plug with cord. For the 230V/50HZ version, make sure the cord and plug conform to local electrical codes. -
Page 10: Lid, Coil Assembly, And Ln Sensor
CG-400/CG-450 Freezer/Mill 4.2 Power Switch and Controller (Cont’d) Grasp controller from the rear and gently pull forward to USB Port adjust viewing angle (back of controller) Figure 3 - Adjusting the Viewing Angle of the Controller 4.3 Lid, Coil Assembly, and LN Sensor The lid of the CG-400/CG-450 Freezer/Mill supports the coil (dual electromagnet assembly) and coil housing. -
Page 11: Grinding Vial Sizes, Adapters, And Vial Openers
CG-400/CG-450 Freezer/Mill 4.3 Lid, Coil Assembly, and LN Sensor (Cont’d) The liquid nitrogen (LN) sensor is the small tube mounted on the left wall of the LN tub. If there is not enough liquid nitrogen in the tub to start the mill when the run button is touched, LN LEVEL LOW screen (Section 5.4) will display LN LEVEL LOW. -
Page 12: Control Panel
CG-400/CG-450 Freezer/Mill 5.1 Logo Screen (Cont’d) The Home Screen Icon can be found on the following screens (e.g. Control Panel, Saved Protocols, Run History, Settings) positioned at the top right of the screen. Touching the Home Icon allows the users to return to Home Screen. Figure 4 –... - Page 13 CG-400/CG-450 Freezer/Mill 5.2 Control Panel (Cont’d) Figure 5 – Control Panel The following sequence of events will occur from the current settings in Figure 5 1. Precool - the coil is inactive while the vial cools in liquid nitrogen for 1 minute. Samples must be precooled before grinding (10 minutes is recommended).
- Page 14 CG-400/CG-450 Freezer/Mill 5.2 Control Panel (Cont’d) Open the lid and place the loaded sample vial in Coil Vial Opening, then SLOWLY close the lid to keep the liquid nitrogen from splashing or spilling out the tub. Two additional loaded sample vials can be placed in the precooling basket while running the sample.
- Page 15 CG-400/CG-450 Freezer/Mill 5.2 Control Panel (Cont’d) During the PRECOOL stage the TIME REMAINING line counts down the time for that stage in 1-second increments. To pause the program, touch the pause button. To continue the program touch the RESUME button. To abort the program, touch the stop button. To start a new program, touch the run button.
- Page 16 CG-400/CG-450 Freezer/Mill 5.2 Control Panel (Cont’d) Figure 9 – Run Complete Screen 5.2.2 Adjusting the Grinding Rate While in the CONTROL PANEL screen, the only parameter that can be modified during grinding is the RATE. It is adjusted by touching the minus (-) and plus (+) buttons next to the Rate field. Touching the minus (-) button increases the rate by 1 cps at a time, while touching the plus (+) button decreases the rate by 1 cps.
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Page 17: Changing Settings
CG-400/CG-450 Freezer/Mill 5.3 Changing Settings To change parameters (precool time, grinding time, cool down time, cycles, and rate), touch the plus (+) and minus (-) buttons on the CONTROL PANEL screen. The changes will appear in the gray area between the (-) and (+), as shown in Figure 10. The CONTROL PANEL allows the user to change the parameters by touching the minus (-) and plus (+) buttons next to the field. - Page 18 CG-400/CG-450 Freezer/Mill 5.4 Saved Protocols (Cont’d) Touch inside the Label Box Figure 11 – Saved Protocols Screen 5.4.1 Storing a new program To store the new program or to recall a stored program, touch the store/recall button on the Control Panel screen (Figure 5). In the SAVED PROTOCOLS screen touch inside the Label Box, as shown in Figure 12.
- Page 19 CG-400/CG-450 Freezer/Mill 5.4 Saved Protocols (Cont’d) The CANCEL key does not change anything on the screen, but returns the display to the SAVED PROTOCOLS screen. To run the newly saved protocol, touch the protocol to highlight the name. Then touch the LOAD button to send protocol to the CONTROL PANEL screen.
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Page 20: Operation
CG-400/CG-450 Freezer/Mill 5.4.3 Edit Saved Protocols To edit a saved protocol, touch the protocol to highlight the name. Then touch the EDIT button. From the Edit Protocol screen change parameters and/or name, then touch the DONE button. 5.4.4 Recalling a program In the SAVED PROTOCOLS screen, touch the protocol to highlight the name. -
Page 21: Loading Samples Into Freezer/Mill Vials
CG-400/CG-450 Freezer/Mill 6.1 Adding Liquid Nitrogen Coolant (Cont’d) If the Freezer/Mill tub is empty, pour liquid nitrogen (LN) into the tub until it is approximately on third full. Initially LN will boil off vigorously, but as the tub cools the boiling will subside. Add more LN gradually, to avoid splashing and boiling over, until the LN level is approximately 2 inches below the rim of the tub. - Page 22 CG-400/CG-450 Freezer/Mill Table 1. Typical Sample Sizes Sample Large Vial Mid-Size Vial Small Vial Microvial 6801, 6803, 6881, 6883, 6885 6751, 6761, 6757 6871 6771, 6781S Plant tissue, bone, 20-50 g 5-20 g 2-5 g 100-300 mg muscle, etc. Polymer pellet 10-20 g 2-10 g 1-2 g...
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Page 23: Loading A Vial Into The Freezer/Mill
CG-400/CG-450 Freezer/Mill 6.2 Loading Samples into Freezer/Mill Vials (Cont’d) CAUTION: Never force an end plug (warm or cold) into a polycarbonate cylinder (warm or cold. Polycarbonate shrinks when chilled, and can crack if stressed. If the cylinder has cooled to the point that the end plug fit is too tight, allow the cylinder to warm up before inserting the end plug. -
Page 24: Opening, Emptying, And Cleaning Freezer/Mill Vials
CG-400/CG-450 Freezer/Mill 6.5 Opening, Emptying, and Cleaning Freezer/Mill Vials 6.5.1 Opening a Vial To open the large vial, slip the open end of the 6804 Extractor/Vial Opener over the flanged end- plug, align the pegs in the end plug with the slots in the Extractor, and turn the knob clockwise until the end plug is drawn out. - Page 25 CG-400/CG-450 Freezer/Mill 6.5 Opening, Emptying, and Cleaning Freezer/Mill Vials (Cont’d) The impactor will have to be separated from the sample and can be handled with gloved fingers, a strong magnet, tongs, or pliers. Tools without a good gripping surface are likely to slip off the impactor.
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Page 26: Checking The Liquid Nitrogen Level
CG-400/CG-450 Freezer/Mill 6.5 Opening, Emptying, and Cleaning Freezer/Mill Vials (Cont’d) The steel parts of the chromium-free 6771, 6871, and 6883 vials can also be washed, disinfected, or autoclaved. Do not wash with bleach or chemical cleaners. Chromium-free steel is not rust- resistant and must always be dried immediately after washing. -
Page 27: Liquid Nitrogen Connection
CG-400/CG-450 Freezer/Mill Note: LN tanks come in two types, high-pressure and low-pressure. Some LN tanks have valve systems to switch from low pressure to high pressure. Always use a low-pressure LN supply, with a delivery pressure of 20-22 psi. 7.2 CG-450 Liquid Nitrogen Connection Install the safety valve on the outlet of the tank or LN line, with the gooseneck tilted up and the valve outlet down (see Figure 14). -
Page 28: Run History
CG-400/CG-450 Freezer/Mill 8.0 Run History To recall Run History touch the Run History button. The Date, Time, and Run Protocol data are stored on this screen, as shown in Figure 15. The run information can be exported to other computer devices via the USB Port located on the back of the Controller. To export run data touch the Export History button at the bottom right of the screen. -
Page 29: Maintenance
CG-400/CG-450 Freezer/Mill 10.0 MAINTENANCE The CG-400/CG-450 Freezer/Mill has been designed to provide trouble-free operation over a long period of time. To ensure proper performance it is very important to keep the unit clean. When the Freezer/Mill is at room temperature, any spilled powders or liquids should be wiped up immediately. -
Page 30: Trouble-Shooting Guide
CG-400/CG-450 Freezer/Mill 11.0 TROUBLE-SHOOTING GUIDE PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION No current. Make sure power cord is plugged into outlet and inlet. No current. Check outlet for power cord and correct as The unit does not turn on. required. Power switch not turned on. Press power switch to ON position. -
Page 31: Warranty
CG-400/CG-450 Freezer/Mill 12.0 WARRANTY Cole-Parmer® guarantees its products against defects in materials or workmanship for three years from the date of original shipment. Repairs, replacements, or parts are guaranteed for 30 days or for the remaining original warranty period (whichever is greater) for the item that was repaired or replaced. -
Page 32: Instrument Disposal
CG-400/CG-450 Freezer/Mill 13.0 INSTRUMENT DISPOSAL In accordance to the EU Directive 2012/19/EU covering Waste Electrical and Electronic Equipment, all equipment with the disposal symbol must not be disposed of with general waste. (See Figure 17) Disposal Label is located on the back of unit. Figure 17 –... -
Page 33: Contact Us
CG-400/CG-450 Freezer/Mill 14.0 CONTACT US Repair Service Phone: 1.732.623.0465 Cole-Parmer 65 Liberty St Metuchen, NJ 08840 Attn: Service and Repair Please include RA Number on the shipping label. Germany T: +1.800.323.4340 or T: +49 (0) 9377 92030 +1.800.323.4340 E: de.sales@antylia.com E: sales@antylia.com W: coleparmer.de W: coleparmer.com...
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