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Summary of Contents for Motic B1 Series
- Page 1 B1 SERIES Biological Microscope Instruction Manual If the equipment is used in a manner not specified by the manufacturer, the protection provided by the Note equipment may be impaired. WWW.MOTIC.COM MOTIC INCORPORATION LTD. E250223...
- Page 2 We are constantly endeavouring to improve our instruments and to adapt them to the requirements of modern research techniques and testing methods. This involves modification to the mechanical structure and optical design of our instruments. Therefore, all descriptions and illustrations in this instruction manual, including all specifications are subject to change without notice.
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Page 3: Table Of Contents
TABLE OF CONTENTS MICROSCOPE TERMINOLOGY Safety Instructions 1.1 General safety instructions 1.2 Instrument safety 1.3 Unpacking, transportation & storage 1.4 Waste disposal 1.5 Operation 1.6 Quality Warranty Nomenclature SETTING UP THE INSTRUMENT 3.1 Operating environment 3.2 Verifying input voltage Assembling The Microscope 4.1 Illumination 4.2 Specimen holder 4.3 Objectives... - Page 4 Microscope Handling 5.1 Illumination brightness adjustment 5.2 Interpupillary distance adjustment 5.3 Diopter adjustment 5.4 Beam splitter lever (trinocular version) 5.5 Coarse and fine focusing 5.6 Coarse focus torque adjustment 5.7 Stage Upper Limit Stop adjustment: 5.8 Use of aperture diaphragm Photomicrographic Procedure Using Oil Immersion Objective Troubleshooting Table...
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Page 5: Microscope Terminology
MICROSCOPE TERMINOLOGY Depth of Focus Abbe Condenser The axial depth of the space on both sides of the A two-lens sub-stage condenser located below image plane within which the image is sharp. The the stage of a microscope and functions to collect larger the N.A. - Page 6 Diopter adjustment Nanometer (nm) The adjustment of the eyepiece of an instrument A unit of length in the metric system equal to 10 to provide accommodation for the eyesight meters. differences of individual observers. Working Distance Real field of view This is the distance between the objective front The diameter in millimetres of the object field.
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Page 7: Safety Instructions
1.SAFETY INSTRUCTIONS 1.1 General safety instructions Please be sure to read these instructions before using microscope. Additional information is available upon request from our maintenance department or authorized agency. To ensure safe operation and guarantee good performance of the microscope please pay attention to the precautions and warnings specified in the Operation Instructions. -
Page 8: Operation
If there is any electrical failure (of the fuse system, ground protection or transformer), turn off and unplug the unit immediately. Make sure the microscope is set aside so it will not be used again and contact the Motic service department or a Motic microscope repair agency to have it repaired. -
Page 9: Quality Warranty
1.6 Quality Warranty The microscope and the attached accessories are only allowed to be used for microscope examination as described in this manual. The manufacturer takes no responsibility for any other use. ● The manufacturer guarantees that the product is free from any defect in material or workmanship on the date of delivery. -
Page 10: Nomenclature
2.NOMENCLATURE MOTIC B1 (Binocular) -
Page 11: Setting Up The Instrument
3.SETTING UP THE INSTRUMENT The microscope shall be placed only on a firm, flat workbench for operation. Avoid placing the instrument in locations exposed to direct sunlight, dust, vibration, high temperature, high humidity and where it is difficult to unplug the power supply cord. Please do not touch the optical lens surfaces. -
Page 12: Assembling The Microscope
4.ASSEMBLING THE MICROSCOPE The components for microscopes are shipped detached for protection. Open the Styrofoam packing with care and do not leave any components attached to the packing being removed. Do not discard any of the packing materials until all of the components have been identified. If any damage occurs during transit, contact both the carrier and your supplier immediately. - Page 13 ● Disconnect the LED connection cables from the power supply printed circuit board. ● Loosen LED board locating ring, take away LED board locating ring. Install the new LED ● Take out a qualified LED groups with power and PIN, nest the pressure ring and reverse action steps as above.
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Page 14: Specimen Holder
4.2 Specimen holder Attach the specimen holder, using the two mounting holes. 4.3 Objectives Lower the stage completely by turning the coarse focus knob. Screw the objectives into the revolving nosepiece so that clockwise rotation of the nosepiece brings the next higher magnification objective into position. -
Page 15: Microscope Handling
5.MICROSCOPE HANDLING 5.1 Illumination brightness adjustment Turn the brightness adjustment knob fully clockwise to the low brightness position. Set the power switch to “I” (ON). The green line control lamp in the switch must light up. When the Brightness adjustment knob is turned counterclockwise to the high brightness position, the light intensity increases. -
Page 16: Beam Splitter Lever (Trinocular Version)
● Correct the focus for the second eye by using only the diopter ring, do not use the coarse/fine focusing knob! ● Change to a higher magnification to verify the result and if necessary repeat the procedure to match the sharpness for higher magnification. ●... -
Page 17: Coarse Focus Torque Adjustment
Never attempt either of the following actions, since doing so will damage the focusing mechanism: ● Rotate the left and right knob while holding the other. ● Turning the coarse and fine focus knobs further than their limit. 5.6 Coarse focus torque adjustment To increase the torque, turn the torque adjustment ring located behind the left-hand coarse focus knob in the direction indicated by the arrow. -
Page 18: Use Of Aperture Diaphragm
5.8 Use of aperture diaphragm ● The condenser aperture diaphragm is provided for adjusting the numerical aperture (N.A.) of the illuminating system of the microscope, it decides the resolution of the image, contrast, depth of focus and brightness. ● Stopping down will lower the resolution and brightness but increase the contrast and depth of focus. -
Page 19: Photomicrographic Procedure
6.PHOTOMICROGRAPHIC PROCEDURE To ensure vibration free operation, set the microscope on a sturdy vibration free table or a bench with a vibration proof device. ● Pull the bean splitter lever of the trinocular eyepiece tube all of the way out to the limit. ○... -
Page 20: Using Oil Immersion Objective
Oil immersion objectives are labelled with the additional engraving “Oil” and are to be immersed in oil between the specimen and the front of the objective. The immersion oil supplied by Motic is synthetic, non-fluorescing and non-resining oil, with a refractive index of 1.515 Normally, cover glass must be used with oil immersion objectives with a few exceptions. -
Page 21: Troubleshooting Table
8.TROUBLESHOOTING TABLE As you use your microscope, you may occasionally experience a problem. The troubleshooting table below contains the majority of frequently encountered problems and the possible causes. 8.1 Optical Problem Possible Cause Bulb not installed properly Condenser not mounted correctly Condenser is set too low Vignetting or uneven brightness in the field Aperture diaphragm closed too far... -
Page 22: Electrical
Dried stain or oil on objective lens. Lens must be removed from the microscope and examined with a Poor image (low contrast or resolution) magnifier (an inverted eyepiece can be used) to check for dirt Stage installed on inclined plane Specimen holder not fixed securely on stage Specimen not secured in position Uneven focus... -
Page 23: Care And Maintenance
Proper handling of the microscope will ensure years of trouble free service. ● If repair becomes necessary, please contact your Motic agency or our Technical Service direct. Note: If equipment is used in a manner not specified by the manufacturer, the warranty may be void. -
Page 24: Warning Labels
Don’t pick the microscope up from the bottom during equipment operation. Proper handling of the microscope will ensure years of trouble free service. If repair become necessary, please contact your Motic agency or our Technical Service directly... - Page 25 Fax: 86-0592-562 7855 © 2007-2016 Motic China Group Co., Ltd. All rights reserved. Motic is a registered trademark and service mark of Motic China Group Co., Ltd. Microsoft Windows logo is a registered trademark of Microsoft Corporation. All other trademarks are the property of their respective owners.
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