Buchi Sepiatec SFC-660 Operation Manual
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Buchi Sepiatec SFC-660 Operation Manual

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Sepiatec SFC-660
Operation Manual

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Summary of Contents for Buchi Sepiatec SFC-660

  • Page 1 Sepiatec SFC-660 Operation Manual...
  • Page 2 CH-9230 Flawil 1 E-Mail: quality@buchi.com BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experience, especially with respect to structure, illustrations and technical details. This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for competitive purposes, nor made available to third parties.

  • Page 3: Table Of Contents

    Electric and electronic connections ................  17 3.2.3 CO₂- and coolant connections ................ 19 3.2.4 Exhaust connections .................... 19 Scope of delivery ......................... 20 Technical data ........................ 20 3.4.1 Sepiatec SFC-660 .................... 20 3.4.2 Ambient conditions .................... 21 3.4.3 Solvents........................ 21 3.4.4 Detectors ........................  22 3.4.5 Pumps ........................
  • Page 4 Using the emergency switch.................... 58 7.10 Monitoring the system functionality .................. 58 Cleaning and servicing ..................... 59 Cleaning the UV detector flow cell.................. 59 Removing the UV detector flow cell.................. 59 Back pressure regulator valve ..................... 60 Fractionation valve ...................... 60 Gas-liquid-separators (GLS).................... 60 Operation Manual Sepiatec SFC-660...
  • Page 5 Table of contents Help with faults ........................ 62 Troubleshooting........................ 62 Taking out of service and disposal..................  63 10.1 Taking out of service ...................... 63 10.2 Disposal.......................... 63 10.3 Returning the instrument ..................... 63 Appendix .......................... 64 11.1 Spare parts and accessories .................... 64 Operation Manual Sepiatec SFC-660...

  • Page 6: About This Document

    Connected instruments In addition to this operation manual, follow the instructions and specifications in the documentation for the connected instruments. 6/66 Operation Manual Sepiatec SFC-660...

  • Page 7: Safety

    — Safety-related incidents that occur while using the instrument should be reported to the manufacturer (quality@buchi.com). BUCHI service technicians Service technicians authorized by BUCHI have attended special training courses and are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair measures.

  • Page 8: Personal Protective Equipment

    The instrument has been developed and manufactured using the latest technological advances. Nevertheless, risks to persons, property or the environment can arise if the instrument is used incorrectly. Appropriate warnings in this manual serve to alert the user to these residual dangers. 8/66 Operation Manual Sepiatec SFC-660...

  • Page 9: Faults During Operation

    Do not continue to use glass components that are damaged. Always wear protective gloves when disposing of broken glass. 2.7.6 Malfunction of a connected instrument (option) A malfunction on a connected instrument can cause poisoning or death. Operation Manual Sepiatec SFC-660 9/66...

  • Page 10: Malware Infection Due To Connections With Other Devices Or Network

    Unauthorized modifications can affect safety and lead to accidents. Use only genuine BUCHI accessories, spare parts and consumables. Carry out technical changes only with prior written approval from BUCHI. Only allow changes to be made by BUCHI service technicians. BUCHI accepts no liability for damage, faults and malfunctions resulting from unauthorized modifications.
  • Page 11 300 bar. NOTE The system my be pressurized after switching off. Keep in mind, the escape of pressurized gases and solvents are possible. Operation Manual Sepiatec SFC-660 11/66...

  • Page 12: Product Description

    With the optional solvent selection valve, the solvent can be chosen from four different solvents. — Injection of liquid or solid sample — Separation on a column — Detection of the components by using a UV, ELSD or MS detector. — Collecting the desired fractions 12/66 Operation Manual Sepiatec SFC-660...
  • Page 13 The Detection and Fraction valve position decides in which GLS the stream is going. The GLS separates most of the CO and removes it from the system by an exhaust pipe and fed into the ventilation system. Operation Manual Sepiatec SFC-660 13/66...

  • Page 14: Injection Procedure

    Injection procedure The pressure in the sample loop can be released into a waste bottle in a controlled manner before loading and the pressure surge into the receiving bottle can be avoided. Injection Decompression Loading Injection 14/66 Operation Manual Sepiatec SFC-660...

  • Page 15: Configuration

    Büchi Labortechnik AG Product description | 3 Configuration 3.2.1 Front view Injection syringe Detector flap Control panel cover Touchscreen and modifier pump Fraction bottles Chiller Column oven Operation Manual Sepiatec SFC-660 15/66...
  • Page 16 3 | Product description Column oven Flow cell with optic fibre cable Injection valve Check valves Heating fan Temperature sensor Modifier filter filter Heating module Leak sensor 10 Mixed stream filter 11 Separation column 12 Column holder 16/66 Operation Manual Sepiatec SFC-660...

  • Page 17: Electric And Electronic Connections

    Büchi Labortechnik AG Product description | 3 3.2.2 Electric and electronic connections Fuses FI-circuit breaker Main switch Operation Manual Sepiatec SFC-660 17/66...
  • Page 18 Büchi Labortechnik AG 3 | Product description USB connection USB connection HDMI connection LAN internal connection LAN external connection Emergency switch connection 18/66 Operation Manual Sepiatec SFC-660...

  • Page 19: Co₂- And Coolant Connections

    — On the left side is the exhaust of the fractionation compartment. — On the right side is the outlet of the CO safety exhaust valve. — On the rear side is the GLS CO exhaust. Operation Manual Sepiatec SFC-660 19/66...

  • Page 20: Scope Of Delivery

    OD: 1/8” ID: 1/16” OD: 1/8” ID: 2.1 mm OD: 1/4” ID: 4.6 mm Pump head temperature control Chiller Method development On analytical columns (ID: 4 − 4.6 mm) Column dimension ID: 4 − 16 mm 20/66 Operation Manual Sepiatec SFC-660...

  • Page 21: Ambient Conditions

    (please see technical specifications of the pump). Check that the following solvents are replenished in sufficient quantity for the desired operating time: — Modifiers — Solvents for flushing the pumps Intended use Solvent Operation Manual Sepiatec SFC-660 21/66...

  • Page 22: Detectors

    Modifier/Add-on pump: 250 ml pump: 500 ml Flow rate range 0.1 − 150 ml/min Flow rate accuracy ± 2% at 2 − 50% of the flow range with methanol (water 80/20 v/v) Pressure Max. 400 bar 22/66 Operation Manual Sepiatec SFC-660...

  • Page 23: Chiller

    Circulation pump max. delivery 29 l/min Min. ambient temperature 5°C Max. ambient temperature 40°C Power supply The power is supplied via the instrument. For further details on the power supply, please refer to the manufacturer's man- ual. Operation Manual Sepiatec SFC-660 23/66...

  • Page 24: Transport And Storage

    The instrument should be transported by two persons at the same time. Lift the instrument at the points indicated. Lift the instrument – this requires two persons each lifting at one of the points indicated on the bottom of the instrument. 24/66 Operation Manual Sepiatec SFC-660...

  • Page 25: Installation

    Make sure that the power supply can be disconnected at any time in an emergency. A BUCHI service engineer or an authorized representative will unpack and check the consignment carefully to ensure that all modules and accessories are in proper condition.
  • Page 26 (laboratory supply, nitrogen generator or cylinder). — It is required to cool the pump head of the Prep SFC 660 CO pump with an air- cooled chiller to a constant temperature of 5°C independent of the ambient temperature. 26/66 Operation Manual Sepiatec SFC-660...

  • Page 27: Before Installation

    Risk of instrument damage because of not suitable power supply cables. Not suitable power supply cables can cause bad performance or an instrument damage Use only BUCHI power supply cables. Precondition: R The electrical installation is as specified on the type plate.

  • Page 28: Software

    ð The system checks if all the functional elements are responding properly. 6.1.2 Entering values Enter numbers Tap on an entry field. ð The display shows a dialog box with a numeric input box. Enter the value. 28/66 Operation Manual Sepiatec SFC-660...

  • Page 29: Exiting The Software

    Exiting the software Precondition: R Run is finished. R Pumps are switched off. Tap the [EXIT] button to exit the software. ð Prep SFC control software closes. Layout Name Description To display the measured values. display Chromatogram Operation Manual Sepiatec SFC-660 29/66...

  • Page 30: Manual Tab

    [Windows] button To exit the control software. Opens the win- dows home screen. MANUAL tab Name Description To start and stop a run manually. MANUAL [FILL SYRINGE] button To rinse the tubing to the syringe. 30/66 Operation Manual Sepiatec SFC-660...

  • Page 31: Rinsing The Tubing And Syringe

    Before or during an isocratic method run in after starting the manual mode. By tapping the [STOP PUMP] button a new window opens and allows you to change the parameters or to stop the pump. Operation Manual Sepiatec SFC-660 31/66...

  • Page 32: Setting Detector Signal To Zero

    COLLECTION tionation parameters can be adjusted for automated further separations. 6.3.3 Setting detector signal to zero Navigation path: ➔ [SERVICE] MANUAL ➔ Tap the [AUTO ZERO] button. ð The detector signal is set to zero. 32/66 Operation Manual Sepiatec SFC-660...

  • Page 33: Changing Co₂ Recycling Module

    The parameter menu has two isocratic modes depending on the configuration: — UV detector — DAD detector (optional) Name Description To set, load and edit parameter. PARAMETER Equilibration Column equilibration time in minutes. Run Time Run time for the separation in minutes. Operation Manual Sepiatec SFC-660 33/66...

  • Page 34: Setting New Methods

    ð A new window opens to choose a solvent from a list or add a new one. Set the parameters on the left part of the window. Optional detector Tap the WAVELENGTH [...] button to set the number of wavelengths. 34/66 Operation Manual Sepiatec SFC-660...

  • Page 35: Setting Gradient Mode

    Tap the [OK] button to confirm and return to parameter menu. Tap the [CANCEL] button to discard the changes and return to parameter menu. ð The programmed gradient is displayed. Operation Manual Sepiatec SFC-660 35/66...

  • Page 36: Loading Saved Methods

    Tap the [Load] button to open the selected method. 6.4.4 Editing saved methods A method that has been saved and opened can be processed as described under Chapter 6.4.1 "Setting new methods", page 34. This method must be saved again before starting it. 36/66 Operation Manual Sepiatec SFC-660...

  • Page 37: Collection Tab

    To start and stop stack injection. Stack time The time between one injection and the next. Start peak detect time To starts the detection mode and control the peaks. Peak control To set parameters for multiple injections. Operation Manual Sepiatec SFC-660 37/66...
  • Page 38 Up to eight fractions in total can be collected. All parts of a run that are not to be collected will be captured in the waste container. Peak control window Name Description Use Peak Control Switch peak control on and off. Controlled Peak Number of the controlled peak. 38/66 Operation Manual Sepiatec SFC-660...
  • Page 39 If the peak is smaller, the run is automatically stopped. The minimum height is in percentage. If the peak is smaller, the run is automatically stopped. Operation Manual Sepiatec SFC-660 39/66...
  • Page 40 Chromatogram of the first injection. Multiple injections Chromatogram of multiple injections. Stack injections Chromatogram of stack injection. Stack time The time between one injection and the next. Start second injection Time when the second injection takes place. 40/66 Operation Manual Sepiatec SFC-660...

  • Page 41: Setting Peak Control

    Tap the [OK] button to confirm. 6.5.2 Setting stack injection The stack injection in isocratic mode allows time saving fractionation of large samples. The sample portions are injected after the elution of a peak. Navigation path: COLLECTION ➔ Operation Manual Sepiatec SFC-660 41/66...

  • Page 42: System Tab

    Back Pressure Indicates the back pressure in the system in bar. Thermostat Temperature in the heat exchanger in °C. Preheater Temperature in the preheater in °C. 42/66 Operation Manual Sepiatec SFC-660...

  • Page 43: Controlling The Back Pressure

    The white field shows the status of the CO valve. Navigation path: SYSTEM ➔ Precondition: R Displayed CO VALVE status: Closed. Tap the [OPEN] button. ð The valve opens. CO VALVE status changes to Open. Operation Manual Sepiatec SFC-660 43/66...

  • Page 44: Controlling The Purge Pump

    Select the modifier inlet and the flow rate. Tap the [Pump Start] button to start the process. Tap the [Pump Stop] button to close the purge valve at the pump and exit purge. 44/66 Operation Manual Sepiatec SFC-660...

  • Page 45: Run Method Tab

    Peak control is on. The peak being monitored ange) has moved and the collection window was adjusted automatically. [PEAK CONTROL] button Peak control is on. The peak being monitored (red) is outside the collection range and the run was stopped. Operation Manual Sepiatec SFC-660 45/66...

  • Page 46: Before Starting A Run

    The button allows changing parameters dur- ing the run. 6.7.1 Before starting a run Navigation path: RUN METHOD ➔ Tap the [Start] button. ð The display shows a dialog with an alphanumeric input box. Enter sample name. 46/66 Operation Manual Sepiatec SFC-660...

  • Page 47: Changing During A Run

    6.8.1 Change No. of Injections Navigation path: ➔ [Change] RUN METHOD ➔ NOTICE! This window close automatically after 10 seconds if no selection is made. Operation Manual Sepiatec SFC-660 47/66...

  • Page 48: Change Injection And Run Time Parameter

    RUN METHOD ➔ NOTICE! This window close automatically after 10 seconds if no selection is made. Tap the [Injection] or [Run Time] button to change parameters. ð The display shows a dialog with a numeric input box. 48/66 Operation Manual Sepiatec SFC-660...

  • Page 49: Change Fraction Parameter

    NOTICE! The method for the fractionation (time and/or peak based) as well as the Start peak detect time can not be changed after the run is started. These buttons are just for information. Operation Manual Sepiatec SFC-660 49/66...

  • Page 50: Co₂ Recycling Module

    ➔ [SERVICE] MANUAL ➔ Name Description To start and stop a run manually. MANUAL [EMERGENCY STOP] button To perform the EMERGENCY STOP. [BACK TO MAIN VIEW] but- To go back to the MANUAL tab view. 50/66 Operation Manual Sepiatec SFC-660...
  • Page 51 [Close all Fraction Valves] This button closes all fractional valves for a button service on the recycling module. Software Version Displays the current software version. Operation Manual Sepiatec SFC-660 51/66...
  • Page 52 This value indicates that fractionation is in progress and how long the fraction valve in is still open. Functional description If the Recycling Inlet Pressure and Recycling Outlet Pressure are approximately equal, the CO pump is supplied with recycled CO 52/66 Operation Manual Sepiatec SFC-660...
  • Page 53 It will close and open the lower valve (fraction valve out) to transfer the fractionated liquid to the collection container. Operation Manual Sepiatec SFC-660 53/66...

  • Page 54: Operation

    Tap the [Verify] button to ensure that all modules are communicating correctly. Positioning of the sample NOTE The capillary tubes should descend in a straight line and without tension. Place the sample in the sample tray. 54/66 Operation Manual Sepiatec SFC-660...

  • Page 55: Co₂ Exhaust Air Control (Collection Bottle)

    In tandem mode, GLS 1 and 2, 3 and 4, 5 and 6 as well as 7 and 8 are connected in parallel. In tandem mode, the fraction tubes that belong together (e.g. GLS 1 and 2 for fraction 1) must be connected to a common fractionation bottle. Operation Manual Sepiatec SFC-660 55/66...

  • Page 56: Method Selection

    15 minutes has been reached. If a stable temperature profile has not been established after 15 minutes, the run is automatically stopped. Navigation path: PARAMETER ➔ 56/66 Operation Manual Sepiatec SFC-660...

  • Page 57: Finishing A Run

    ð All injections that are already on the column (stack injection) will be finished. Then the system stops. The caption of the automatic start button changes to stopped. Tap the [Immediately] button. ð The run stops immediately. Operation Manual Sepiatec SFC-660 57/66...

  • Page 58: Shutting Down The System

    If the system is idle the watchdog time is 10 minutes, if the pumps are running this time is reduced to 2 minutes. During normal shutdown of the software, the heating elements are switched off, but the emergency power circuit is not switched off. 58/66 Operation Manual Sepiatec SFC-660...

  • Page 59: Cleaning And Servicing

    Do not carry out any servicing and cleaning operations that involve opening the housing. Use only genuine BUCHI spare parts in order to ensure correct operation and preserve the warranty. Carry out the service and cleaning operations described in this section to extend the lifetime of the instrument.

  • Page 60: Back Pressure Regulator Valve

    Remove the detector from the detector compartment. Back pressure regulator valve Please contact the BUCHI Customer Service if any problems occur with the back pressure regulator valve. Fractionation valve Please contact the BUCHI Customer Service if any problems occur with the fraction valve.
  • Page 61 (e.g. acetone). Be careful not to bend the input capillary of the GLS. Place the GLS cups. Screw the GLS cups counter-clockwise with your hands. Tight the GLS cups hand-tight with a 16 mm wrench. Operation Manual Sepiatec SFC-660 61/66...

  • Page 62: Help With Faults

    See pump manual. Pressure fluctuation Check valves are dirty or Clean or replace check valves. worn out See pump manual. Set temperatures Defective heating elements Contact BUCHI Customer are not reached Service Defective temperature con- trol 62/66 Operation Manual Sepiatec SFC-660...

  • Page 63: Taking Out Of Service And Disposal

    When disposing, observe the disposal regulations of the materials used. Materials used see Chapter 3.4 "Technical data", page 20. 10.3 Returning the instrument Before returning the instrument, contact the BÜCHI Labortechnik AG Service Department. https://www.buchi.com/contact Operation Manual Sepiatec SFC-660 63/66...

  • Page 64: Appendix

    Appendix 11.1 Spare parts and accessories Use only genuine BUCHI consumables and spare parts in order to ensure correct, safe and reliable operation of the system. NOTE Any modifications of spare parts or assemblies are only allowed with the prior written permission of BUCHI.
  • Page 66 We are represented by more than 100 distribution partners worldwide. Find your local representative at: www.buchi.com...

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