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Related Manuals for THORLABS Telesto Series
Summary of Contents for THORLABS Telesto Series
- Page 1 SD-OCT Base Unit Spectral Domain OCT System Base Units: Telesto, Ganymede, and Callisto Series User Manual...
- Page 2 Original User Manual – not translated...
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Page 3: Table Of Contents
Chapter 3 Description ........................ 1 6 3.1. Tutorial ........................16 Theory ........................... 17 Thorlabs SD-OCT System Technology ................. 18 Polarization-Sensitive Optical Coherence Tomography ..........19 Nomenclature in OCT imaging ..................20 ... - Page 4 8.3. Warranty Exclusions ....................58 Chapter 9 Specifications ....................... 5 9 Chapter 10 Mechanical Drawings .................... 6 1 Chapter 11 Regulatory ........................ 6 2 11.1. Waste Treatment is Your Own Responsibility ............62 11.2. Ecological Background ..................62 Chapter 12 Thorlabs Worldwide Contacts .................. 6 3 ...
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Page 5: Chapter 1 Introduction
Laser Safety symbol indicates that laser radiation is present. This manual describes the SD-OCT base units of the Telesto, Ganymede, and Callisto Series. If not specified, the base units of the Telesto-PS Series are incorporated in the Telesto Series. ATTENTION Check the supply voltage of the system before plugging in the computer. -
Page 6: Safety
Make sure that the line voltage rating agrees with your local supply and that the appropriate fuses are installed. Fuses should only be changed by qualified service personnel. Contact Thorlabs for assistance. Do not operate without cover installed. Refer servicing to qualified personnel. -
Page 7: Care And Maintenance
If the system is mishandled during shipment, the optical components may become misaligned, which could lead to a decrease in image quality. If this occurs, the system will need to be realigned by qualified personnel. Please contact Thorlabs technical support for more information. ... -
Page 8: Optical Cleaning
Good performance and image quality of the OCT imaging system relies on clean optical connections. Whenever using the Thorlabs OCT system, the following guidelines for optical fiber connection should be followed: 1) Always make sure that the light source is switched off when you clean the fiber. - Page 9 SD-OCT Base Unit Chapter 1: Introduction Cleaning Fiber Bulkheads Figure 3 Cleaning Fiber Bulkheads Remove the guide cap completely from the device, and insert the tip of the cleaner into the bulkhead connector. Push the case to start the cleaning process; a click indicates that the cleaning is complete. Cleaning Fiber Connectors Figure 4 Cleaning Fiber Connectors Open the cover on the guide cap, and insert the fiber connector over the guide cap.
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Page 10: Service
Thorlabs provides. Any modification or maintenance by unqualified personnel will render the warranty null and void, leaving Thorlabs free of liability. Please contact Thorlabs technical support for questions on customization. -
Page 11: Chapter 2 Setup
C. Fiber Connection to the Imaging Scanner (FC/APC) In Telesto-PS Series base units a fiber cable is permanently installed D. Auxiliary Fiber Connection to Imaging Scanner (FC/APC) – Not in Telesto Series and some Ganymede Series base units E. Probe Connection Port (LEMO, 19 Pin) F. -
Page 12: Electrical Interfaces To Probe
Electrical Interfaces to Probe For the connection to a scanner application there are two different interfaces available. The probe connection port is intended to be used together with dedicated Thorlabs imaging scanners OCTG and OCTP. The auxiliary connection port is intended to be used together with dedicated Thorlabs imaging scanner OCTH and furthermore allows the use of a custom scanner. -
Page 13: System Installation
SD-OCT Base Unit Chapter 2: Setup 2.4. System Installation ATTENTION Make sure the included power cords for the base unit, computer and monitor are connected to a properly grounded outlet (100 – 240 VAC; 50 – 60 Hz). Transportation and delivery may cause the OCT base unit to be warm or cool upon receipt. Please wait for the system to reach room temperature before attempting to operate. - Page 14 SD-OCT Base Unit Chapter 2: Setup 4) Connect the CameraLink and the SMB cables between the base unit and the PC (not for CAL-Series base units). a. In the Telesto-PS Series base units connect the long SMB cable between the base unit and the SMB tee connector which is plugged into the PC.
- Page 15 SD-OCT Base Unit Chapter 2: Setup 7) Attach the electric connection cable to the imaging scanner. Align the red dot of the plug to the alignment mark of the electric connection port of the scanner (e.g.: OCTG). Figure 8 Plugging the Electric Connector into the Scanner Figure 9 Electric Connector Plugged into the Scanner Push the connector into the receptacle until a “click”...
- Page 16 SD-OCT Base Unit Chapter 2: Setup 8) Connect the fiber to the imaging scanner. ATTENTION When installing the fiber, make sure that the fiber tip does not get contaminated by dust. Do not touch the fiber tip! Remove the dust caps from one fiber end and from the FC/APC fiber connection at the imaging scanner.
- Page 17 SD-OCT Base Unit Chapter 2: Setup 9) Attach the electric connection cable to the base unit. Align the red dot upwards, facing the alignment mark in the base unit. Figure 11 Installing the Electric Connection Cable at the Base Unit Push the connector into the receptacle until a “click”...
- Page 18 SD-OCT Base Unit Chapter 2: Setup 10) Connect the fiber to the base unit. (Not in Telesto-PS Series base units.) ATTENTION When installing the fiber, make sure that the fiber tip does not get contaminated by dust. Do not touch the fiber tip! Remove the dust caps from the fiber end and from the FC/APC fiber connection at the base unit.
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Page 19: Enable External Trigger
SD-OCT Base Unit Chapter 2: Setup 11) Pull the protective cap off the scan objective. Do not touch the optical surface of the lens. Figure 13 Protective Cap Removal Enable External Trigger To enable the option to use an external trigger, a tee connector must be inserted into the trigger connection to allow inserting the trigger signal. -
Page 20: Chapter 3 Description
Spatially-encoded Frequency Domain OCT (seFD-OCT), also named Fourier Transform Domain OCT (FD-OCT) and Spectral Domain OCT (SD-OCT). Thorlabs uses the abbreviations SD-OCT for the spatially-encoded camera-based OCT systems and SS-OCT for the time-encoded systems. In both types of systems, light is split by a fiber coupler into the sample and reference arms of an interferometer setup. -
Page 21: Theory
SD-OCT Base Unit Chapter 3: Description Theory The interference equation for the cross-correlated interference term is ~2 ∙ ∙ ∙ cos Δ With the phase difference Δ being a function of the optical path length difference and the wavenumber Δ ∙... -
Page 22: Thorlabs Sd-Oct System Technology
To gain access to this back-scattered light, it is necessary to insert a splitting device into the optical path. In Thorlabs' SD-OCT systems, this device could be either a fiber coupler or a circulator. -
Page 23: Polarization-Sensitive Optical Coherence Tomography
SD-OCT Base Unit Chapter 3: Description Polarization-Sensitive Optical Coherence Tomography A standard OCT system is able to measure a depth scan of the sample using the phase and intensity of the backscattered light from different positions in depth. Nevertheless, it does not account for polarization effects or birefringence in the sample which might be interesting if the sample shows different birefringent properties locally. -
Page 24: Nomenclature In Oct Imaging
SD-OCT Base Unit Chapter 3: Description Based on the intensity and phase differences in the interference patterns detected by both sensors, net polarization changes as a function of depth in the sample are determined with the typical spatial resolution of an SD-OCT system. - Page 25 SD-OCT Base Unit Chapter 3: Description When scanning both galvanometer mirrors, a volume can be acquired. This can be imaged by movable sections through the volume or by 3D rendering. Please refer to the OCT Software Manual for all features available. Figure 21 Rendered Volumetric Data Set Figure 22 En-Face View or C-Scan When displaying a plane with both scan directions as axes, an en-face image is created.
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Page 26: Oct Base Unit Components
A scanner as well as the OCT-STAND are not included in the base unit and must be ordered separately. When ordered together with a scanner, the system will be pre-assembled at Thorlabs and the PC settings will already be calibrated and optimized for the usage of the delivered OCT scanner. -
Page 27: Sdk
Hardware control through the SDK ranges from low-level functions such as setting the galvo scanners to a desired position, to very powerful functions such as initiating full 3D measurements. The programmer can: • Define either a standard scanner provided by Thorlabs or create a software representation of a custom- built device. •... -
Page 28: Imaging Scanner (Accessory)
Chapter 3: Description Imaging Scanner (Accessory) Thorlabs SD-OCT systems use a common-path OCT setup in which the interferometer is located within the imaging scanner. This integration of the interferometer eliminates the problems associated with chromatic or polarization mode dispersion that are introduced by differences between individual fibers in the sample and reference arms. - Page 29 SD-OCT Base Unit Chapter 3: Description Figure 26 OCTP-PS User-Customizable PS OCT Scanner Figure 27 OCTH Handheld Scanner Rev E, November 7, 2018 Page 25...
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Page 30: Oct-Stand (Accessory)
SD-OCT Base Unit Chapter 3: Description OCT-STAND (Accessory) The Thorlabs OCTG and OCTP scanners can be mounted to an OCT-STAND. For this OCT-STAND, the rotation-and-translation stage OCT-XYR1 is available. Figure 28 OCT-STAND with OCT-XYR1 Figure 29 OCT-STAND Adjuster Page 26... - Page 31 SD-OCT Base Unit Chapter 3: Description Figure 30 OCT-XYR1 Sample Rotation Stage For further details on the OCT-STAND and the OCT-XYR1 please refer to the Thorlabs web site. Rev E, November 7, 2018 Page 27...
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Page 32: Chapter 4 System Operation
Wait for 30 seconds until the PC has recognized the hardware. 2) Start the Thorlabs OCT software (see software user’s manual for details). The system will be switched on via remote control from the computer. After starting the software, the green “SYS OK” LED on the base unit will illuminate. -
Page 33: Adjusting The Reference Intensity
SD-OCT Base Unit Chapter 4: System Operation Adjusting the Reference Intensity For optimum imaging quality it is necessary to ensure that the reference intensity is set into the correct range. The reference intensity is displayed by the OCT software, as shown below. For optimizing the reference intensity pull out the reference intensity adjustment knob (see Figure 24). - Page 34 SD-OCT Base Unit Chapter 4: System Operation Figure 34 B-Scan of a Fingertip Out of Focus and In Focus In Figure 34, two images of a fingertip are shown: the left is out of focus and the right is in focus. The focus can usually be identified by one of more of the following features: ...
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Page 35: Advanced Adjustments
Figure 35 OCT B-Scans of Scattering Particles taken with LSM02, LSM03, and LSM04 Thorlabs offers three scan lens kits for different purposes. Figure 35 shows the difference in lateral resolution and depth of focus for the OCT-LK02 (high resolution imaging), OCT-LK3 (general purpose), and OCT-LK4 (long depth of focus). -
Page 36: Imaging Through Refractive Media
Thorlabs offers a special reference arm adapter to further increase the reference arm length, please contact Thorlabs for more information. -
Page 37: Optimizing The Sensitivity
SD-OCT Base Unit Chapter 4: System Operation Optimizing the Sensitivity In Frequency-Domain OCT systems, a fall-off of the sensitivity is observed at larger measurement depths. This is especially obvious in SD-OCT systems where this so-called roll-off starts to affect measurements already at around 30% of the maximum measurement depth. -
Page 38: Rough Surfaces
(e.g. a glass slide) to reduce scattering. Thorlabs offers sample z-spacers that provide a glass plate at a fixed distance to reduce scattering effects and to keep the sample in focus, please contact Thorlabs for more information. -
Page 39: Example Images
SD-OCT Base Unit Chapter 4: System Operation 4.5. Example Images Frequency Domain OCT can be used for a wide range of real-time monitoring applications in biological and clinical fields as well as in manufacturing and materials science. This technology is ideal for in-line industrial imaging applications ranging from laminated packaging films to 3D visualization of mechanical parts. - Page 40 SD-OCT Base Unit Chapter 4: System Operation Material Imaging SD-OCT can also be used for non-biological material science applications. Such systems are ideal for monitoring surface topography and layered structures. Figure 42 B-Scan of a Semi-Transparent Molded Plastic Cap Figure 43 B-Scan of a Laminated IR Card Figure 44 B-Scan of plastic.
- Page 41 SD-OCT Base Unit Chapter 4: System Operation Biological Imaging Figure 45 B-Scan of a Section of a Grape Figure 46 B-Scan of carious tooth. Top – Intensity-Based OCT image, Bottom - Degree Of Polarization Uniformity (DOPU)-Based OCT image Rev E, November 7, 2018 Page 37...
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Page 42: Saturation And Non-Linearity
SD-OCT Base Unit Chapter 5: Imaging Artifacts Chapter 5 Imaging Artifacts 5.1. Saturation and Non-Linearity The OCT A-scan data is created by frequency analysis of the spectral data generated by the spectrometer. Intense reflection from the sample can saturate the sensor of the spectrometer or illuminate very close to saturation. -
Page 43: Wrong Reference Intensity Setting
SD-OCT Base Unit Chapter 5: Imaging Artifacts When operating with a wedge, the image will be tilted in the direction of the wedge angle. When scanning in the orthogonal direction, no tilt occurs. 5.2. Wrong Reference Intensity Setting The OCT image is created by interferometry as shown in Figure 17. For good image acquisition, the intensity of the reference light needs to be well above noise level and well below saturation. -
Page 44: Autocorrelation
SD-OCT Base Unit Chapter 5: Imaging Artifacts 5.3. Autocorrelation The fundamental principle of SD-OCT is a frequency analysis of an interference signal entering the spectrometer. In the usual case, this interference signal is created by photons returned from the sample interfering with photons returned from the reference arm. -
Page 45: Multiple Scattering
SD-OCT Base Unit Chapter 5: Imaging Artifacts 5.4. Multiple Scattering When imaging highly scattering material, a large portion of the photons returned to the detection system have been scattered multiple times from travelling into the sample until exiting. Since OCT visualizes the relative travelled path lengths of photons, signals from multiple scattered photons are shown deeper in the image than physically present. -
Page 46: Phase Wrapping And Fringe Washout
SD-OCT Base Unit Chapter 5: Imaging Artifacts 5.5. Phase Wrapping and Fringe Washout The A-scan data created by the SD-OCT system is produced from spectral information of an optical interference. Depending on the system setting, a certain integration time is applied for acquisition of each A-scan. Certain movement of the sample or parts of it can well be detected by comparing the phase information of adjacent A- scans. -
Page 47: Flipped Image
SD-OCT Base Unit Chapter 5: Imaging Artifacts 5.6. Flipped Image Without the introduction of additional techniques not provided by the standard SD-OCT system, there is no distinguishing between photons that traveled a distance d shorter or longer from the beam splitter to the sample compared to the reference arm length. -
Page 48: Shadowing
SD-OCT Base Unit Chapter 5: Imaging Artifacts 5.7. Shadowing Since the SD-OCT imaging uses light for detection of depth information, one can only see information from regions in the sample, where photons are transmitted to and allowed back into the sampling aperture. Reflections, strong scattering and absorption lead to shadows in the depth distribution of the data acquired. -
Page 49: Image Distortion By Refractive Media
SD-OCT Base Unit Chapter 5: Imaging Artifacts 5.8. Image Distortion by Refractive Media OCT images display path length differences in between reference arm length and sample arm length (distance from the beam splitter to the scattering or reflecting object). These path lengths are optical path lengths, calculated from the physical path length multiplied by the group refractive index. -
Page 50: The Group Refraction Index
SD-OCT Base Unit Chapter 5: Imaging Artifacts The Group Refraction Index The principle of optical coherence tomography is the detection of optical path length differences between the two arms of an interferometer. The optical paths within these arms are defined by the mechanical path lengths and the refractive indices of the materials. -
Page 51: Measurement Depth In Oct Systems
SD-OCT Base Unit Chapter 5: Imaging Artifacts Measurement Depth in OCT Systems The spectral resolution of a frequency domain OCT system defines its possible measurement depth. This depth is the maximum detectable optical path length difference limited by the Nyquist criteria. In real materials the measurement depth of OCT systems as well as the axial resolution is reduced. -
Page 52: Distortions In The Image
SD-OCT Base Unit Chapter 5: Imaging Artifacts Distortions in the Image In complex structures, distortions occur in the OCT image which require a close look to be understood. Figure 61 Different Materials in One Measurement The loss of imaging depth depends on the amount of material through which the beam passes. As a result the measured depth in the sample changes throughout the scan. - Page 53 SD-OCT Base Unit Chapter 5: Imaging Artifacts As an example, a material with wedge is analyzed: Figure 62 Complex Structure in Image The block shows “standard” behavior on the right side where the surface is perpendicular to the incoming beam. In the chamfered area there is diffraction and the beam travels under an angle through the block.
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Page 54: Chapter 6 Troubleshooting
OCT image Adjust reference intensity Reference intensity too high or too low knob Other reason Call Thorlabs Reference length set incorrectly Adjust reference length Flipped Image Table 3 Troubleshooting Please refer to Chapter 12 for Thorlabs contact information. Page 50 MTN012389-D02... -
Page 55: Changing The Input Fuses
SD-OCT Base Unit Chapter 6: Troubleshooting 6.1. Changing the Input Fuses If for some reason you need to replace an open fuse in the base unit, you must perform the following procedure: Remove the AC input cable that may be connected to the unit. ... -
Page 56: Chapter 7 Certifications And Compliance
SD-OCT Base Unit Chapter 7: Certifications and Compliance Chapter 7 Certifications and Compliance 7.1. Declaration of Conformity GAN2x0 Series Base Units Page 52 MTN012389-D02... -
Page 57: Declaration Of Conformity Gan6X0 Series Base Units
SD-OCT Base Unit Chapter 7: Certifications and Compliance 7.2. Declaration of Conformity GAN6x0 Series Base Units Rev E, November 7, 2018 Page 53... -
Page 58: Declaration Of Conformity Tel2X0 Series Base Units
SD-OCT Base Unit Chapter 7: Certifications and Compliance 7.3. Declaration of Conformity TEL2x0 Series Base Units Page 54 MTN012389-D02... -
Page 59: Declaration Of Conformity Tel2X0Ps Series Base Units
SD-OCT Base Unit Chapter 7: Certifications and Compliance 7.4. Declaration of Conformity TEL2x0PS Series Base Units Rev E, November 7, 2018 Page 55... -
Page 60: Declaration Of Conformity Tel3X0 Series Base Units
SD-OCT Base Unit Chapter 7: Certifications and Compliance 7.5. Declaration of Conformity TEL3x0 Series Base Units Page 56 MTN012389-D02... -
Page 61: Declaration Of Conformity Cal1X0 Series Base Units
SD-OCT Base Unit Chapter 7: Certifications and Compliance 7.6. Declaration of Conformity CAL1x0 Series Base Units Rev E, November 7, 2018 Page 57... -
Page 62: Chapter 8 Warranty
Warranty 8.1. Lasers and Imaging Systems Thorlabs offers a one year warranty on all lasers and imaging systems, with the exceptions of laser diodes. 8.2. Non-Warranty Repairs Products returned for repair that are not covered under warranty will incur a standard repair charge in addition to all shipping expenses. -
Page 63: Chapter 9 Specifications
SD-OCT Base Unit Chapter 9: Specifications Chapter 9 Specifications General Performance Specifications – SD-OCT Base Unit 100 V – 240 V / AC Supply Voltage for Base Unit 150 W Maximum Power Consumption 12.5 kg Weight Base Unit 10 °C to 35 °C Storage/Operating Temperature 206 mm x 305 mm x 248 mm Dimensions of OCT-STAND (L x W x H) - Page 64 SD-OCT Base Unit Chapter 9: Specifications Optical Performance Specifications – Callisto Series Base Unit CAL110 Base Unit 930 nm Central Wavelength 1.2 KHz Axial Scan Rate 1.7 mm / 1.3 mm Maximum Imaging Depth Air/Water (typical) 7.0 µm / 5.3 µm Axial Resolution Air/Water (typical) 8.0 µm (OCT-LK3-BB)
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Page 65: Chapter 10 Mechanical Drawings
SD-OCT Base Unit Chapter 10: Mechanical Drawings Chapter 10 Mechanical Drawings Figure 64 Base Unit Dimensions Rev E, November 7, 2018 Page 61... -
Page 66: Chapter 11 Regulatory
11.1. Waste Treatment is Your Own Responsibility If you do not return an “end of life” unit to Thorlabs, you must hand it to a company specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site. -
Page 67: Chapter 12 Thorlabs Worldwide Contacts
SD-OCT Base Unit Chapter 12: Thorlabs Worldwide Contacts Chapter 12 Thorlabs Worldwide Contacts For technical support or sales inquiries, please visit us at www.thorlabs.com/contact for our most up-to- date contact information. USA, Canada, and South America UK and Ireland Thorlabs, Inc. - Page 68 M0009-510-1071-D...
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