GE AKTA pilot 600 User Manual page 62

4 Operation
4.1 Sample application
4.1.1 Background considerations
Option
Apply sample from
a Superloop
Sample application phase
A Sample Application phase is included in all predefined chromatography methods. The
sample will be automatically applied to the column when the method is run. Selections
in the Sample Application phase determine how the sample will be applied. The illustra-
tion below shows the phase in a method outline.
The following sections describe how to use the settings in the Phase Properties for the
Sample Application phase. For detailed information on each setting, press the F1 key
to display the UNICORN help.
62
When?
Recommended for sam-
ple volumes in the range
10 to 150 mL.
Description
A Superloop (sold separately) is
manually filled with sample and
connected to one of the two col-
umn positions. The sample is inject-
ed onto the column by directing
the system flow via the Superloop,
and does not pass through any
other modules in the system.
Sample volumes applied from a
Superloop are limited to the vol-
ume of the Superloop (150 mL).
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