Filling The Testing Capillary With A Normal Volume Sample - MES SQA-iO Service Manual

Service Manual Version 187.5.1
APPENDIX 3: Filling the Testing Capillary with a Normal Volume Sample
Sample size, collection and preparation instructions:
1. Sample volume should be at least .5 ml. If sample volume is less than .5 ml
the sample can be run as a short sample following the instructions in
Appendix 2.
2. The sample must be collected without partners, gels or creams and tested
within 1 hour of collection for accurate results.
3. Do not heat or refrigerate the sample, maintain an test at room temperature
4. The semen sample must be completely liquefied and well mixed prior to
aspiration: Gently mix by rotating the sample collection container.
WARNING: Do not shake or use a pipette to mix the sample otherwise air
bubbles will form and test results will be inaccurate.
5. Carefully check that the liquefied, fully mixed specimen is free of air bubbles (or that there is an
adequate amount of sample below the air bubbles) before immersing the capillary into the specimen.
Filling the capillary:
1. Push the syringe pump fully into the syringe. Place only thin part of the
capillary into the bottom of the sample while angling the sample container at
about 45 degrees (Figure 1).
2. Placing two fingers below the syringe pump's head pull the syringe pump back
slowly while keeping the tip of the capillary well below the sample level
and below any surface bubbles (Figure 1). Continue to aspirate the sample
until it appears in the Luer adaptor (Figure 2).
AUGUST 3, 2021 SQA-iO Service Manual 11
Figure 1
Figure 2