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Teledyne 340CF ELSD Installation And Operation Manual

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340CF ELSD
Installation and Operation Guide
Part #69-5243-095
Copyright © 2006
Revision G, July 25, 2016

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Summary of Contents for Teledyne 340CF ELSD

  • Page 1 340CF ELSD Installation and Operation Guide Part #69-5243-095 Copyright © 2006 Revision G, July 25, 2016...
  • Page 3 être affaiblie; cela augmentera votre risque de blessure. WARNING If this system uses flammable organic solvents, Teledyne Isco recommends that you place this system in a well-ventilated environment, designed for these types of materials.
  • Page 4 AVERTISSEMENT Ce système peut utiliser des dissolvants organiques inflammables. Pour réduire le péril qui peut être causé par l'accumulation des vapeurs explosives, Teledyne Isco recommande que vous installez ce système dans un environnement bien-aéré qui est conçu pour les matières hasardeuses.
  • Page 5 ELSD Safety The equipment and this manual use symbols used to warn of Hazard Symbols hazards. The symbols are explained below. Hazard Symbols Warnings and Cautions The exclamation point within the triangle is a warning sign alerting you of important instructions in the instrument’s technical reference manual. The lightning flash and arrowhead within the triangle is a warning sign alert- ing you of “dangerous voltage”...
  • Page 6 ELSD Safety...

  • Page 7: Table Of Contents

    3.2.8 Nebulization Chamber Drain ........3-2 3.3 Connection to Teledyne Isco Instruments ....... . 3-3 3.3.1 Connecting to the CombiFlash®...
  • Page 8 2-1 340CF ELSD Specifications ........

  • Page 9: Connecting To The Combiflash® Rf200

    340CF ELSD Section 1 Quick Start Guide This section provides information specific to connecting the ELSD unit to a Teledyne Isco instrument. For general infor- mation about installing the ELSD, refer to Section 3.2. 1.1 Connecting to the The CombiFlash Rf200 uses RFID (Radio Frequency Identifi- CombiFlash®...

  • Page 10: Connecting The Rf Ports To The Elsd

    340CF ELSD Section 1 Quick Start Guide 183cm 60cm Figure 1-2 Connecting the Rf ports to the ELSD 4. Connect a 65 ±5psi inert gas supply (such as nitrogen) to the brass fitting on the lower left rear panel of the ELSD, with "...

  • Page 11: Rf External Detector Cable Connections

    340CF ELSD Section 1 Quick Start Guide Ground ELSD Connection Rf200 Connection Ground (CU) Figure 1-4 Rf external detector cable connections...

  • Page 12: Connecting To The Combiflash® Torrent

    340CF ELSD Section 1 Quick Start Guide 1.2 Connecting to the The CombiFlash Torrent system is designed to handle a variety of large-scale purification needs, with various methods of sample CombiFlash® Torrent introduction and collection. Connection of the Torrent to the ELSD requires the connection package, part #60-5247-021.
  • Page 13 340CF ELSD Section 1 Quick Start Guide Disconnect Figure 1-6 Torrent: disconnect tubing from bottom column connection 5. Locate the black PTFE tubing that is swaged on both ends. Connect one end to the Torrent bottom column connection. Connect the other end to the metal tee fitting on the back of the ELSD.

  • Page 14: Nebulizationchamber Drain

    340CF ELSD Section 1 Quick Start Guide 1.3 NebulizationChamber Attach the stainless steel drain tube to the nebulization chamber drain port located on the front of the instrument. Direct the Drain outlet of the tube to a collection vessel and empty the vessel when full.

  • Page 15: Section 2 Introduction

    MS systems. 2.2 Servicing A periodic maintenance kit is available from Teledyne Isco (part #60-5247-024). The kit contains instructions, parts, and equipment for servicing the ELSD. Contact the factory for more information.

  • Page 16: Nebulization

    340CF ELSD Section 2 Introduction 2.3.1 Nebulization Nebulization transforms the liquid phase leaving the column into an aerosol cloud of fine droplets. The size and uniformity of the droplets are extremely important in achieving sensitivity and reproducibility. The ELSD uses a special concentric flow nebu- lizer and a constant flow of an inert gas to ensure a narrow droplet size distribution.

  • Page 17: Evaporation

    340CF ELSD Section 2 Introduction 2.3.2 Evaporation After passing through the nebulization chamber, the aerosol cloud is propelled through the heated evap- oration tube, assisted by the carrier gas. In the e v a p o r a t i o n t u b e , t h e...

  • Page 18: Specifications

    340CF ELSD Section 2 Introduction 2.4 Specifications Table 2-1 340CF ELSD Specifications Dimensions 10" W x 18.5" D x 11.5" H Weight 25 lbs Display 2 Line x 20 Character per line VFD User Interface Four multi-function keys Evaporative Zone Temperature Ambient to 120 °C...

  • Page 19: Section 3 Installation

    3.2 Connections The following sections provide general connection information for the ELSD unit. For information about connecting the ELSD to Teledyne Isco instruments, refer to Section 3.3. 3.2.1 Power The ELSD operates at 120V or 234V, 50/60 Hz. Confirm that the ELSD is configured for the correct voltage before plugging it into line voltage.

  • Page 20: Digital I/O Connections

    340CF ELSD Section 3 Installation 3.2.5 Digital I/O Pin 1 and Pin 2: Input for remote control of pump (refer to Connections Section 4.3). Also a contact closure output. An error conditions exists when pins are open. Pin 3 and pin 4: A contact closure input to turn on/off gas remotely.

  • Page 21: Connection To Teledyne Isco Instruments

    340CF ELSD Section 3 Installation 3.3 Connection to This section provides information specific to connecting the ELSD unit to a Teledyne Isco instrument. Teledyne Isco Instruments 3.3.1 Connecting to the The CombiFlash Rf200 uses RFID (Radio Frequency Identifi- CombiFlash® Rf200 cation) technology to automate setting the parameters for purifi- cation runs and fraction collection.

  • Page 22: Connecting Gas Supply To Elsd

    340CF ELSD Section 3 Installation 4. Connect an inert gas supply (such as nitrogen) to the brass fitting on the lower left rear panel of the ELSD, with " O.D. line. Figure 3-4 Connecting gas supply to ELSD 5. Connect the external detector cable (Isco part #250-0000-20) to the Rf200 (Figure 3-5).

  • Page 23: Connecting To The Combiflash® Rf200 With 4X Module

    340CF ELSD Section 3 Installation 3.3.2 Connecting to the 1. Place the ELSD next to the Rf200, opposite the 4X module, CombiFlash® Rf200 as shown in Figure 3-6. with 4X Module 2. Connect the 4X tubing to the Rf200, except for the tubing marked "D,"...

  • Page 24: Connecting To The Combiflash® Torrent Module

    Module introduction and collection. The Torrent can be configured for use with Teledyne Isco’s 340CF ELSD. The data connection between the Torrent and the ELSD must be made via the DETECTOR port on the rear panel of either a Teledyne Isco Foxy R2 fraction collector or a Teledyne Isco frac- tionation valve.

  • Page 25: Elsd Interior

    340CF ELSD Section 3 Installation PTFE tubing Drift tube Pump motor Tape (cut) PTFE tubing pump Liquid Inlet connection plug (hidden behind gusset) 4" nuts (4) Figure 3-9 ELSD Interior 2. Remove the four " nuts holding the gusset in place.
  • Page 26 340CF ELSD Section 3 Installation Figure 3-11 Disconnect PTFE line from pump 6. Route the line from the pump out through the Liquid Inlet opening in the front panel. 7. Disconnect the harness from the pump motor. Figure 3-12 Disconnect pump harness...

  • Page 27: Operating Temperatures

    340CF ELSD Section 3 Installation 3.4.1 Operating Temperatures Table 3-1 Suggested Operating Temperatures for HPLC Gradient Separations Solvent @ 1.0mL/min. Drift Tube Temperature, °C Spray Chamber Temperature, °C 90/10 Water/METHANOL 90/10 Methanol/WATER 50/50 Methanol/Water Acetonitrile Methanol Water 3.4.2 Shut Down Procedure For HPLC applications, the ELSD must count down to the standby state.

  • Page 28: Installation Qualification Checklist

    340CF ELSD Section 3 Installation 3.5 Installation Table 5-1 may be completed to verify and document the installation procedures contained in this section of the manual. Qualification Checklist Table 5-1 Installation Qualification Checklist Installer Operator Step Description Initials Initials Unpacking...

  • Page 29: Section 4 Operation

    340CF ELSD Section 4 Operation 4.1 Instrument Controls The ELSD is controlled via four multi-function keys on the front ▲ ▼ panel labeled , and . The ELSD MENU POWER AUTOZERO features a 2 line vacuum florescent display and a row of status lights to aid in the operation of the detector.

  • Page 30: Autozero Key

    340CF ELSD Section 4 Operation other keys. Once the ELSD is powered up the MENU POWER is used to view error conditions, cycle through the menu, accept changes to set points, enter standby mode, and turn the power off. Error condition exist when the ready light is not illuminated and when the * appears in the upper right corner of the display.
  • Page 31 340CF ELSD Section 4 Operation When the ELSD finishes the start-up process it enters the stand-by mode. Press the key to exit and enter the MENU POWER HOME Screen. ELSD is in Standby Mode Push PWR to exit Diagnostics Complete...
  • Page 32 340CF ELSD Section 4 Operation RST/BLN Screen ELS Detection ELS Detection 20.000mV Baseline 20.000mV 221.22 When RST is selected from the menu screen, it will display “RST Baseline” for 5 seconds. Pushing key during this MENU POWER period will reset the ELS Detection signal to about 20mV. [Using key can also reset the baseline.] After 5 seconds,...
  • Page 33 340CF ELSD Section 4 Operation Spray Chamber Page SPRAY CHAMBER 10.0°C 10°C The reading under SPRAY CHAMBER is the current temper- ature. The reading under SET is the setpoint. ▲ ▼ Pushing keys to change a setpoint at 1°C increment.
  • Page 34 340CF ELSD Section 4 Operation ready or from ready to not ready. The alarm is 10 short beeps repeated every 30 seconds. Pressing the key resets the AUTOZERO alarm. Timer Page STANDBY COUNTDOWN DEPRESS PWR button 5 Set: 05min 05:00...
  • Page 35 340CF ELSD Section 4 Operation Load Method Page LOAD METHOD: 0 1 2 3 4 5 6 7 8 9 If the number selected has no method saved, M ETHOD will be displayed. If the non-volatile memory IC chip VAILABLE is damaged, the same message will be displayed.
  • Page 36 340CF ELSD Section 4 Operation Detector Gain Page AMPLIFIER GAIN: HPLC Prep ▲ ▼ keys to select an action. Push key to take an action and go back Home MENU POWER screen. Push key to keep the current state and go back Home AUTOZERO screen.

  • Page 37: Operation

    340CF ELSD Section 4 Operation 4.2 Operation If this is the first time you have operated the detector, please refer to the Quick Start Guide. 4.2.1 Start Up Procedure It is very important that you reproduce the QC tests found in the Quick Start Guide before beginning your analysis.

  • Page 38: Spray Chamber Temperature Effects - Sub-Ambient

    340CF ELSD Section 4 Operation When setting the ELSD temperatures for a new method, select 25°C for spray chamber temperature and 55°C for drift tube tem- perature. These temperatures should then be adjusted for the best signal to noise ratio during method optimization. For the...

  • Page 39: Spray Chamber Temperature Effects - Elevated

    340CF ELSD Section 4 Operation As the Spray Chamber temperature increases from 0° to 15°C, more of the vapor phase goes to Drift Tube and not to the drain and the signal becomes larger. When the Spray Chamber is heated from 15°C to 25°C the signal decreases because the vapor is partially evaporated in the spray chamber.

  • Page 40: Operating Modes

    340CF ELSD Section 4 Operation Figure 4-3 Drift Tube Temperature Effects The effect of temperature on signal between 45°C and 50°C is small, but as the drift tube temperature increased above 60°C the signal height decreased. A drift tube temperature setting that is too high could vaporize the analyte and cause a loss of sensitivity.

  • Page 41: Automatic Operation (Default)

    340CF ELSD Section 4 Operation 7. Press to return to the home screen. MENU POWER If temperature setpoints are reentered or operating conditions change, the pump stops automatically in either mode. The screen will display the message . Press NOT READY...

  • Page 42: Default Method

    340CF ELSD Section 4 Operation Column Pre-Treatment Chromatographic columns may introduce particles into the mobile phase, which may lead to increased noise and blocked fluid paths. It is recommended that the chromatographic column be flushed with at least 10 column volumes before it is connected to the ELSD.
  • Page 43 4700 Superior, Lincoln, Nebraska 68504 USA Mailing Address: P.O. Box 82531, Lincoln, NE 68501 Equipment Type/Environment: Laboratory Equipment for Light Industrial/Commercial Environments Trade Name/Model No: 330 ELSD or 340CF ELSD Year of Issue: 2010 Standards to which Conformity is Declared: EN 61010-1 2...
  • Page 45 * This warranty applies to the USA and countries where Teledyne Isco does not have an authorized dealer. Customers in countries outside the USA, where Teledyne Isco has an authorized dealer, should contact their Teledyne Isco dealer for warranty service.

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