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Teledyne Isco recommends that you read this manual completely before placing the equipment in service. Although Teledyne Isco designs reliability into all equipment, there is always the possibility of a malfunction. This manual may help in diagnosing and repairing the malfunction.
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Warnings and Cautions The lightning flash and arrowhead within the triangle is a warning sign alerting you to “dangerous voltage” inside the product. The exclamation point within the triangle is a warning sign alerting you to important instructions in this manual. Symboles de Sécurité...
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Warnungen und Vorsichtshinweise Der gepfeilte Blitz im Dreieck ist ein Warnzeichen, das Sie vor „ “ gefährlichen Spannungen im Inneren des Produkts warnt. Das Ausrufezeichen in Dreieck ist ein Warnzeichen, das Sie darauf aufmerksam macht, daß wichtige Anleitungen zu diesem Handbuch gehören.
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STATE, AND LOCAL REGULATORY LAWS, AND IN COMPLIANCE WITH YOUR COMPANY’S CHEMICAL/ HYGIENE PLAN IN THE EVENT OF A SPILL. IN ALL CASES, WHEN USING TELEDYNE ISCO INC. INSTRU- MENTATION, PRUDENCE AND COMMON SENSE MUST BE USED. CAUTION: If this instrument is used in a manner not specified in this manual, the protection provided by the equipment may be impaired.
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AVIS: LES LIQUIDES QUI SONT ANALYSÉS DANS CET INSTRU- MENT PEUVENT ÊTRE CANCÉRIGÈNES, HASARDS BIOLOGIQUES, INFLAMMABLES, OU RADIOACTIFS. SI VOUS DEVEZ UTILISER CES LIQUIDES HASARDEUX, IL EST TRÈS RECOMMANDÉ QUE VOUS LE FAITES À L'INTÉRIEUR D'UN ENVIRONNEMENT ISOLÉ CONÇU POUR TELS LIQUIDES. CET ENVIRONNEMENT ISOLÉ...
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ADDENDUM The chart recorder of the UA-6 can be set to produce charts based on cm/ml length of chart per unit vol- ume pumped instead of the traditional cm/hr (length of chart per unit time). For volume based charts, plug the square 4-pin connector on the cable (part number 60-1020-163 or 60-1020-228) into the socket labeled REMOTE CHART DRIVE on the rear panel of the UA-6.
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When an application problem arises, contact the laboratory director for assis- tance. Our staff will do its utmost in analyzing and solving any problems. If you have developed a unique application for a Teledyne Isco Instrument or are considering a new use, we would be interested in learning about it.
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COMMONLY ORDERED REPLACEMENT PARTS FOR THE TELEDYNE ISCO UA-6 These replacement parts are often ordered by UA-6 owners. All parts are pictured and identified by number and description in Appendix A Replacement Parts in the back of this manual. Description...
The optical units house the lamp, filters and flow cells and are designed to be plugged into the UA-6 and attached to a ring stand, mast or shelf. 1.1 Optical Units for the UA-6 1.1.1 Type 11...
The UA-6 can operate as a UV/VIS computing colorimeter with optional cuvette holders and 10 mm cuvettes. 1.3 Technical The technical specifications of the UA-6 are listed in Table 1-1. Specifications 1.4 Controls and The controls and indicators utilized in the operation of the UA-6...
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UA-6 Detector Section 1 Introduction Table 1-1 Technical Specifications for the UA-6 Dimensions and Weight Width: 11.2 in (28.4 cm) (Detector cabinet only) Height: 6.5 in (16.5 cm) Depth: 13.0 in (33.0 cm) Weight:14.6 lb (6.6 kg) Power Requirements (Factory Set) Mains voltage line cord is a 100 VAC ±...
UA-6 Detector Section 1 Introduction Table 1-1 Technical Specifications for the UA-6 (Continued) Peak Separator Peak Duration Ranges 0.3, 1, 3, 10, 30+ minutes Example: Long Duration Peak One hour base width with a minimum height of 10% of scale...
UA-6 Detector Section 1 Introduction Figure 1-2 UA-6 Front Panel Table 1-2 Front Panel Controls and Indicators Item in Control or Indicator Description Function Fig. 1-2 STANDBY-OPERATE 2 position selector switch In OPERATE, applies secondary power to the unit. Switch In STANDBY, applies secondary power to heaters.
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RED: Type 11 lamp is warming up. Red after warm up indicates lamp current needs to be reset! GREEN: Type 11 lamp current is proper, ready for operation. Figure 1-3 UA-6 Rear Panel...
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Connectors for an external voltage sensitive 1 V; 10 mV; (common recorder. ground); (chassis ground) INTERNAL RECORDER Banana sockets Input for operation of the UA-6 recorder from a INPUT 1 V separate 0-1V instrument. INTEGRATOR Banana sockets For connection of external integrator computer.
Function Fig 1-4 Detector Connect Cable Six individually shielded pairs Provides interface connection to with a 24-pin connector. a Teledyne Isco absorbance detector control system. BASELINE ADJUST-MIN Rotary Control Moves an aperture plate assem- OPEN-MAX OPEN bly back and forth in the light path to meter the light for zero baseline calibration procedures.
2.2 Preparation for Use (Power) CAUTION Prior to connecting the UA-6 to power, check the serial tag to ensure that the voltage and frequency requirements of the instrument are compatible with the available voltage and fre- quency.
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UA-6 Detector Section 2 Preparation for Use Note If the recorder has been sitting overnight, tear off about three inches of chart paper to preclude the possibility of the paper rolling around the drum. To install the pen (refer to Figure 2-1): 1.
UA-6 Detector Section 2 Preparation for Use 2.4 Attachment of the The optical unit is plugged into the UA-6 “OPTICAL UNIT” Optical Unit to the socket as shown in Figure 2-2. The optical unit can then be posi- UA-6 tioned close to the column while the detector/recorder component remains on the lab bench.
Type 11 Optical Unit filters selected must be of the same wavelength and must match the source screen used. When the UA-6 and Type 11 are shipped, filter and source screen for 254 nm are in place. Figure 2-3 Changing Wavelengths in the Type 11 Optical Unit...
(as recommended) it is not necessary that the two flow cells used be of identical pathlengths or even equal cell types. It is recom- mended that the UA-6 always be operated with flow cells in both reference and measuring compartments. Do not operate without...
As a cross-check, an external standard solution can be used to External Standard calibrate the UA-6. Baseline unit at zero. Prepare a solution of potassium chromate. Use a 0.45 AUFS dilution. Place the external standard solution in measuring cell A and 0.05 N KOH in reference cell B.
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10. The baseline setting may be left at mid scale or moved to any arbitrary baseline by using the recorder offset control of the UA-6. Positioning the baseline will not alter the accuracy of the reading. 11. The AUTO BASELINE function can be activated by an external controller also.
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UA-6 Detector Section 2 Preparation for Use...
UA-6 Detector Section 3 Operation 3.1 Introduction The basic operation of the UA-6 consists of selecting the settings for sensitivity, noise filtering, peak separation (if applicable) and the built-in chart recorder. Once the flow cells and filters have been selected and installed, the following steps should be fol- lowed for starting operation.
3.2 Flow Cell Applications The UA-6 has different types of flow cells for use with the Type 1 1 o p t i c a l u n i t : p r e p a r a t i v e c e l l s f o r l o w p r e s s u r e...
UA-6 Detector Section 3 Operation 3.3.1 Preparative Flow Cells The preparative flow cells (Figures 3-1 and 3-2) consist of a body fabricated from a fluoroplastic (Halar®), two optically polished quartz windows and a lead connector screwed into each end of the body.
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UA-6 Detector Section 3 Operation PREPARATIVE FLOW CELL Figure 3-2 Flow Cell Cross-Section Table 3-2 Flow Cell Specifications Preparative High Flow Rate Pathlength (mm) Illuminated Volume (µ) 37.5 Illuminated Volume (µ per cm/light path) Hold Up Volume (µ) Part Number...
UA-6 Detector Section 3 Operation 3.3.2 High Flow Rate Cell The high flow rate cell with a fixed pathlength (5 mm) has the same construction as the preparative cells but has a inch flow path. This cell is capable of working with flow rates up to 3 liters per minute.
Section 3 Operation 3.4 Flow/Pressure Refer to Figure 3-3 for flow versus pressure characteristics of Characteristics Teledyne Isco flow cells. Note 1. Flow rate vs. pressure drop characteristics were compiled using the lead connector that is packaged with each flow cell on the inlet port and no lead connector on the outlet port.
UA-6 Detector Section 3 Operation Figure 3-4 Medium Pressure (400 psi, 28 kg/cm²) Lead Connector Installation 3.6 Tubing Connections The polypropylene lead connectors provided with the high flow for High Flow Rate rate flow cell will accommodate inch OD and inch OD flexible or rigid plastic tubing.
A UA-6 has a 0.1 nm bandwidth at 254 nm and certain other wavelengths, which is excellent. A UA-6 has a 15 nm bandwidth at 280 nm and certain other wavelengths, which is only fair, so shorter pathlength cells are generally better at these wave- lengths.
UA-6 Detector Section 3 Operation 3.7.2 Flow Rate The Teledyne Isco high flow rate cell is designed to handle liquid flows up to about 3 liters/min with negligible pressure drop. Liquids with viscosities much greater than water may present difficulties at high flow rates, while most cells can handle higher flow rates of water or other non-viscous liquids if some pressure drop across the flow cell is not objectionable.
3.9 Noise Filter The UA-6 includes a built-in noise filter with RISE TIME set- tings of 0.5, 1.5 and 5.0 seconds. (RISE TIME is a response speed measurement defined as the time required to make a 10% to 90% pen deflection.) This filter can be used to reduce the noise level...
This paper is printed with a 10 cm wide grid strip with 2 mm minor and 1 cm major divisions. Increasing absorbance causes the pen to deflect to the left. The internal recorder in the UA-6 may be used as a stand-alone recorder (see Section 3.15). 3.11 Built-In Peak...
3.12 Performance and Use This section covers generalized information on several aspects of the UA-6 that can help you use the instrument more effectively. 3.12.1 Minimizing Trace One source of noise is the solvent itself. Certain organic solvents,...
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Drift performance is directly related to the stability of the ambient temperature. The UA-6 can operate at any temperature between 0°C and 40°C without affecting drift, as long as it remains stable. Temperature changes of 1°C will cause noticeable drift at a SENSITIVITY setting of 0.002 AUFS.
The cables are tagged with a seven-digit number. The input and output signals handled by the UA-6 and its rela- tionship to other instruments in a system are shown in Figure 3-10. These instruments are typical of those that can be used with a UA-6.
Cable #69-2134-173 should be used to connect a Teledyne Isco Fraction Collectors Foxy R1 or R2 to the UA-6 for level sensing, slope detection, and tube change event marks (Figure 3-11). This cable has two con- nectors at the UA-6 end, allowing interface to the recorder output (a dual banana plug) or for peak separator input (a 9-pin plug).
UA-6 Detector Section 3 Operation 3.13.2 Teledyne Isco Use cable 60-1020-217 to connect the UA-6 with a Teledyne Isco Retriever 500 Retriever 500 fraction collector. The information transmitted between the two units consists of tube change and rack change event marks.
UA-6 Detector Section 3 Operation Figure 3-13 Suggested Circuit for a Remote Chart Drive For a 60 Hz recorder, chart travel will be: 216000 pulses per cm = recorder range cm/hr For a 50 Hz recorder chart travel will be:...
UA-6 Detector Section 3 Operation Figure 3-14 Problem Analysis - Drift and Noise 3-20...
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UA-6 Detector Section 3 Operation The following traces represent various difficulties as each is noted. All were run with a chart speed of 1.5 and 3 cm/hr with sensitivity at 0.01 AUFS, unless otherwise noted. See Table 5-5, Item 2.
4.1.1 Optical Unit All current UA-6 optical units are dual light path devices which use two flow cells, as depicted in Figure 4-1. The optical units all operate with a single light source which emits the desired ultra- violet and visible spectrum.
Refer to the UA-6 schematics (available online) during the fol- lowing description. To view the schematic drawings for the UA-6, first find the serial number for your unit. Then go to our Web site at www.isco.com. Select Service and then Fast Thorough Factory Repair Service.
UA-6 Detector Section 4 Theory of Operation Note Auto Baseline repeatability varies depending on the sensitivity range used. The following lists a few examples of Auto Base- line repeatability. Sensitivity Range Repeatability 0.002 ±25% of chart 0.02 ±3% of chart ±0.2% of chart...
4.2.8 Lamp Brightness Located in the left center portion of the schematic, this circuit is Regulator only active when UA-6 is used with a Type 11 optical unit. When active, it performs five functions: • 10 minute warm-up cycle. • Limits lamp current to a maximum of 270 mA.
-15 V, regulated by VR401, supplies power to all analog and digital circuitry (VR401 is a linear regulator). When the UA-6 is connected to an AC power source and is in the standby mode, three heater resistors (R449, 516, and 523) warm the unit.
UA-6 Detector Section 4 Theory of Operation In Operate mode, normal circuit operation creates enough heat to prevent condensation. In addition to the main power supplies, there are 2 precision power supplies +10 V and -10 VDC. U212 is a precision +10 V ref- erence utilized to provide very stable voltages to the optical unit photocells.
UA-6 Detector Section 4 Theory of Operation 4.3 Type 11 Optical Unit This section explains the optical unit theory of operation. To Theory of Operation provide a clear, basic understanding of the optical unit circuitry, equivalent circuits and mechanical functional diagrams are used in the following paragraphs.
UA-6 Detector Section 4 Theory of Operation Light radiating through the reflector aperture, then through the measuring flow cell, is filtered and passed to a photodetector. Both filter assemblies, which are matched in wavelength to the source screen, transmit a narrow spectral region. The resultant...
UA-6 Detector Section 4 Theory of Operation The third element is unique to the Teledyne Isco fluorescence filter assembly and improves performance by blocking violet and red stray light that passes through the first element. CONNECTORPINS CHASSISGROUND REFERENCECELL MA TCHEDP AIR...
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UA-6 Detector Section 4 Theory of Operation Figure 4-5 Type 11/Absorbance Detector Block Diagram 4-11...
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UA-6 Detector Section 4 Theory of Operation 4-12...
The procedures are limited to the replacement of specific parts and troubleshooting the detector. For problems more serious than those listed, contact Teledyne Isco's Service Department for help. 5.1 Removal and Installation of the...
UA-6 Detector Section 5 Maintenance and Adjustment 6. Remove 2 screws from case bottom. 7. Remove 1 screw from the top left corner of the circuit boards (it engages the recorder). 8. Carefully remove the recorder from the detector. CAUTION Extreme care must be taken when removing the recorder not to damage any components within the detector.
UA-6 Detector Section 5 Maintenance and Adjustment 5.2.2 Recorder Drive String Read through the following instructions and refer to Figure 5-2 Installation to replace the recorder drive string. 1. Loosen the two stop bracket securing screws and remove the T-shaped stop bracket.
UA-6 Detector Section 5 Maintenance and Adjustment 3. Loosen the two inch set screws which secure the hub to the metal shaft and lift the hub completely off the metal shaft. 4. Loosen the pen holder screw (marked (A) in Figure 5-2), remove it, and take off the first two of the three washers.
Section 5 Maintenance and Adjustment Figure 5-3 Timing Belt Tension Adjustment 5.4 Cleaning the To clean the enclosures of the UA-6 and the optical units, use a Enclosures mild detergent in water. For stains of an organic nature, use iso- propyl alcohol in a sponge that has been mostly squeezed dry.
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Section 5.6.3, and if required, replace it with a serviceable one. i. Defective photocell in the optical unit. i. Return optical unit to Teledyne Isco for photocell replacement. j. Defective AUTO BASELINE switch in the j. Replace switch.
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Probable Cause Solution b. Optical unit has an imbalance greater than b. Set the UA-6 to “SET LAMP & OPTICS” 0.5 absorbance units. and adjust the baseline control on the optical unit until the chart pen zeros. If the chart pen is below 0% of scale, there is a problem with the reference side of the optical unit.
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UA-6 Detector Section 5 Maintenance and Adjustment DANGER Risk of electric shock. Disconnect the electric power before servicing Only trained service personnel may remove the case top. Table 5-2 Recorder Troubleshooting Problem Probable Cause Solution 1. Chart paper does not a.
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UA-6 Detector Section 5 Maintenance and Adjustment Table 5-2 Recorder Troubleshooting (Continued) Problem Probable Cause Solution 5. Recorder does not a. Defective components in the detector a. Using standard electronic techniques, Event Mark at a fraction Event Mark circuitry. repair or replace as required.
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Defective fluorescent or diffusing light c. Replace (see item 7, Figure 5-4). source slide. d. Defective photocells in the optical unit. d. Return optical unit to Teledyne Isco for photocell replacement. 2. Lamp flickers or a. Defective UV lamp in the optical unit, or a.
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UA-6 Detector Section 5 Maintenance and Adjustment 5.6 Type 11 Optical Unit The following sections present the maintenance procedures that Maintenance may be required in the field. These maintenance procedures are limited to replacement of specific parts and troubleshooting without the requirement of a service person or sp ecial equipment.
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6. Carefully install the replacement UV lamp. Note It is strongly recommended that locally purchased UV lamp replacement not be used. All lamps utilized in Teledyne Isco optical units are subjected to very stringent test procedures and approximately 50% are found to be unusable.
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UA-6 Detector Section 5 Maintenance and Adjustment 14. Turn the optical unit OFF. 15. If required, loosen two photocell adjustment screws and adjust the regulating photocell to point at the UV lamp bright spot with a inch clearance between the regulating photocell and the UV lamp glass surface.
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UA-6 Detector Section 5 Maintenance and Adjustment Figure 5-4 UV Lamp and Regulating Photocell Removal and Installation 5-14...
UA-6 Detector Section 5 Maintenance and Adjustment 5.6.3 Filter Assembly If the optical unit malfunctions and the possible cause is a Inspection defective filter assembly (Figure 5-4) it is possible to visually check the filter assembly using the correct light source intended for the wavelength involved.
Check the filter assembly using the proce- tive. dures specified in Section 5.6.3. g. Defective photocell in the optical unit. g. Return the optical unit to Teledyne Isco for photocell replacement. h. Defective absorbance detector control h. Check the detector control system and system.
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Optical unit filter is defective. b. Check the filter using the procedures speci- fied in Section 5.6.3. c. Photocell is defective. c. Return optical unit to Teledyne Isco for pho- tocell replacement. d. Source screen is defective; the light d. Replace the source screen.
Note Refer to the Replacement Parts List in the back of this manual that is applicable to the flow cell in use to obtain the Teledyne Isco part numbers for the replacement items. 5. Moisten the replacement O-ring with distilled water and carefully install on top of the window.
UA-6 Detector Section 5 Maintenance and Adjustment Figure 5-5 Window and O-Ring Removal and Installation 5.7.2 Correcting for Flow The method for correcting leaks that may occur around the flow Cell Window Leakage cell windows is presented in the following paragraphs. This method is applicable to all flow cells: 1.
UA-6 Detector Section 5 Maintenance and Adjustment 5.7.3 Troubleshooting The most common malfunctions that may occur during normal operation of the flow cells are identified in Table 5-6, which iden- tifies the problem, probable cause, and the solution. Table 5-6 Flow Cell Troubleshooting...
5.7.6 Service Department If you have a problem with the instrument or need parts infor- mation, contact Teledyne Isco's Service Department. If you write, please include all pertinent information that may be helpful in solving your problem. Address your letter to: Teledyne Isco, Service Department P.O.
5.7.8 Purchasing Parts Only the parts listed in the Replacement Parts List of this manual are stocked for immediate delivery by Teledyne Isco. Vir- tually all other parts are also available, but there may be some delay in shipping them because they are not normally stocked.
Replacement parts are called out in the following pages. Refer to the call-out in the adjacent table to determine the part number for the item. Replacement parts can be purchased by contacting Teledyne Isco’s Customer Service Department. Teledyne Isco Customer Service Department P.O.
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UA-6 Detector Appendix A Replacement Parts...
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UA-6 Detector Appendix A Replacement Parts...
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UA-6 Detector Appendix A Replacement Parts MAIN CIRCUIT BOARD...
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UA-6 Detector Appendix A Replacement Parts...
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UA-6 Detector Appendix A Replacement Parts...
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UA-6 Detector Appendix A Replacement Parts...
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UA-6 Detector Appendix A Replacement Parts...
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UA-6 Detector Appendix A Replacement Parts...
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UA-6 Detector Appendix A Replacement Parts A-10...
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UA-6 Detector Appendix A Replacement Parts A-11...
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UA-6 Detector Appendix A Replacement Parts A-12...
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UA-6 Detector Appendix A Replacement Parts A-13...
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UA-6 Detector Appendix A Replacement Parts A-14...
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UA-6 Detector Appendix A Replacement Parts A-15...
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UA-6 Detector Appendix A Replacement Parts A-16...
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UA-6 Detector Appendix A Replacement Parts A-17...
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UA-6 Detector Appendix A Replacement Parts This page intentionally left blank A-18...
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UA-6 Detector Appendix A Replacement Parts A-19...
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UA-6 Detector Appendix A Replacement Parts A-20...
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UA-6 Detector Appendix A Replacement Parts Drawing modified 7-6-11 to remove obsolete flow cells A-21...
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UA-6 Detector Appendix A Replacement Parts A-22...
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UA-6 Detector Appendix A Replacement Parts A-23...
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UA-6 Detector Appendix A Replacement Parts A-24...
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UA-6 Detector Index Applications for the UA-6, 1-2 Leakage, Flow Cell Window, 5-19 Baseline Adjustment, 2-6 Noise and Drift, Minimizing, 3-13 Bubble Formation and Retention, 3-10 Noise Filter, 3-11 Cables, 3-16 Optical Unit Attachment, 2-2 Calibration, 2-6 Optical Units, 1-1...
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UA-6 Detector Index Lamp Replacement, 5-11 Theory of Operation, 4-8 Type 12 Fuses, 2-4 Index-2...
Disregard the following Radio Interference Statement and Declara- tion of Conformity if your instrument does not have a CE label on its rear panel. Isco UA-6 RADIO INTERFERENCE STATEMENT NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.
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Name and amount of Hazardous Substances or Elements in the product Hazardous Substances or Elements Component Name (Pb) (Hg) (Cd) (Cr(VI)) (PBB) (PBDE) UV Lamp Circuit Boards AC Motor DC Motor Wiring Line Cord Transformer Name and amount of Hazardous Substances or Elements in the product O: Represent the concentration of the hazardous substance in this component’s any homogeneous pieces is lower than the ST/ standard limitation.
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Mailing Address: P.O. Box 82531, Lincoln, NE 68501 Equipment Type/Environment: Laboratory Equipment for Light Industrial/Commercial Environments Trade Name/Model No: UA-6 with Type 11 & 12 Optical Units Year of Issue: 2000 Standards to which Conformity is Declared: EN 61326-1998 EMC Requirements for Electrical Equipment for...
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68504, U.S.A. customers facility and the repair facility. * This warranty applies to the USA and countries where Teledyne Isco does not have an authorized dealer. Customers in countries outside the USA, where Teledyne Isco has an authorized dealer, should contact their Teledyne Isco dealer for warranty service.