Shodex IEC SP-FT 4A Operation Manual

(Please read this manual carefully to achieve the best and consistent column performance for a long time)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex product. Shodex  IEC SP-FT 4A is a strong cation exchange
column filled with non-porous hydrophilic polymer-based gel modified with sulfopropyl group. Its small
hydrophobic adsorption makes the column suitable for analyzing various proteins, peptides, and nucleic
acids. The polymer-based gel allows its use at a wide pH range condition.

2. Column Components

3. Column Specifications

Product
Product Name
Code
F6113100 IEC SP-FT 4A
Base Material : Non-porous particles of polyhydroxymethacrylate modified with sulfopropyl group
Column Housing : PEEK
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : 20 mM MES
Operation Manual

Shodex IEC SP-FT 4A
Column Size
(mm)
I.D. Length
4.6 10
*
Buffer (pH5.6)
SHOWA DENKO K.K. (https://www.shodex.com/)
Particle Theoretical Plate
Size Number
(μm) (Per Column)
2.7 ≥ 20,000
*2-(N-Morpholino)ethanesulfonic acid
ver.211110E
Ion Exchange
Capacity
(meq/g)
0.2
1