Calibration; Ph Calibration - Fisher Scientific accumet AE150 User Manual

Calibration

pH Calibration

The Fisher Scientific accumet AE150 pH benchtop meter is capable of performing a one to
five point pH calibration for accuracy across the pH measurement range.
The multi-point calibration offers flexibility of calibrating using either USA or NIST buffer sets
with their fixed calibration points:
•
USA Standard Buffer Option: pH 1.68, 4.01, 7.00, 10.01 and 12.45 buffers
•
NIST Standard Buffer Option: pH 1.68, 4.01, 6.86, 9.18 and 12.45 buffers
For ATC temperature measurements, attach the temperature probe to the meter. The "ATC"
icon will be shown on the display. Insert the temperature probe into the solution to be
measured so that the sample temperature can be recorded and pH readings automatically
temperature compensated. If manual temperature compensation is preferred, do not plug a
temperature probe into the meter.
DO NOT REUSE SOLUTIONS AFTER CALIBRATION. Contaminants in the solution can
affect the calibration, and eventually the accuracy of the measurements.
A one point calibration (offset calibration) must be performed using pH 7.00 buffer or pH 6.86
buffer; otherwise calibration will not be accepted.
For slope-to-slope calibration (two point calibration), the first calibration point will only be
successfully registered in the meter after the second point calibration has been completed.
It is recommended that you perform at least a two point calibration using buffers that bracket
the expected sample range (one buffer above the sample pH and one buffer below the
sample pH), starting with the pH 7.00 buffer or pH 6.86 buffer first.
All new calibrations will replace the existing stored calibration data.
1. To power the meter on, press the ON/OFF key. All the display segments will be
displayed briefly and then the pH measurement mode will be displayed.
a. If necessary press the MODE key to select the pH measurement mode.
2. The display will indicate the following:
a. "MEAS" icon indicates the meter is in the measurement mode
b. "pH" icon indicates the pH measurement mode is selected
c. "°C" icon indicates the measured temperature reading
d. "ATC" icon indicates automatic temperature compensation is active when a
temperature probe is connected
3. Rinse the pH electrode with deionized water or rinse solution. If using a separate
temperature probe, thoroughly rinse the temperature probe as well.
a. Do not wipe the pH electrode or temperature probe, as this may cause a
build-up of electrostatic charge that may cause measurement instability.
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accumet AE150 pH Benchtop Meter
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