Olympus U-CBE Instructions Manual page 20

U-CSE
2
Specimen Placement
1. Position the specimen on the rotatable stage (U-SRP), in such a manner that the specimen center portion
coincides with the intersection of the eyepiece cross lines, and focus on the specimen.
3
Adjusting the Fiducial Point
1. Rotate the stage and adjust so that the measured portion on the specimen becomes dark.
2. Rotate the stage by +45° and then clamp it.
3. Pull out the U-CSE from the test plate adapter (U-TAD) or polarization attachment (U-PA), push the test
plate (U-TP530) into the U-TAD or U-PA and confirm that the interference color of the measured portion
changes from the sensitive color to blue. If the color changes to red-orange-yellow instead of blue, rotate
the stage by -90° and then clamp it. Now pull out the test plate from the U-TAD or U-PA.
4
Adjusting the Fiducial Point
1. Place an interference filter in the filter holder on the light exit of the microscope.
2. Rotate the analyzer dial and adjust so that the measuring point on the specimen becomes dark.
3. At this point, read the analyzer's angle. → θ
4. Find the retardation by using the following formula:
17
546 X
Retardation (nm) =
θ – θ
│ │
0
180°