Thermo Scientific CRYO Plus 1 Operating And Maintenance Manual page 58

Liquid nitrogen storage system
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Never use hollow rods or tubes as dipsticks.
When a warm tube is inserted into liquid nitrogen, liquid will spout
from the top of the tube due to gasification and rapid expansion of liq-
uid inside the tube.
Warning Nitrogen gas can cause suffocation without warning!
Store and use liquid nitrogen only in a well-ventilated place.
As the liquid evaporates, the resulting gas tends to displace the normal
air from the area. In closed areas, excessive amounts of nitrogen gas
reduce the concentration of oxygen and can result in asphyxiation.
Because nitrogen gas is colorless, odorless and tasteless, it cannot be
detected by the human senses and will be breathed as if it were air.
Breathing an atmosphere that contains less than 18% oxygen can cause
dizziness and quickly result in unconsciousness and death.
Note The cloudy vapor that appears when liquid nitrogen is exposed to the
air is condensed moisture; not the gas itself. The issuing gas is invisible.
Never dispose of liquid nitrogen in confined areas or places where others
may enter.
Disposal of liquid nitrogen should be done outdoors in a safe place.
Pour the liquid slowly on gravel or bare earth where it can evaporate
without causing damage. Do not pour the liquid on pavement.
First Aid
If a person seems to become dizzy or loses consciousness while working
with liquid nitrogen, move to a well-ventilated area immediately. If
breathing has stopped, apply artificial respiration. If breathing is difficult,
give oxygen. Call a physician. Keep warm and at rest.
If exposed to liquid or cold gas, restore tissue to normal body temperature
(98.6°F) as rapidly as possible, followed by protection of the injured tissue
from further damage and infection.
Remove or loosen clothing that may constrict blood circulation to the
frozen area. Call a physician. Rapid warming of the affected part is best
achieved by using water at 108°F. Under no circumstance should the
water be over 112°F, nor should the frozen part be rubbed either before or
after rewarming. The patient should neither smoke nor drink alcohol.
Section 9
Appendix
7007400 Cryoplus
9-3

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