Forma Scientific 3158 Manual
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Forma Scientific 3158 Manual

Water jacketed incubator
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Forma Scientific, Inc.
P.O. Box 649
Marietta, Ohio 45750
Telephone: (740) 373-4763
________________________________________
Single Chamber 3158, 3164, 3154, 3166
Failure to read, understand and follow the instructions in this manual may result in
damage to the unit, injury to operating personnel and poor equipment performance.
Caution: All Internal Adjustments And Maintenance Must Be Performed By
Qualified Service Personnel.
Telefax: (740) 373-4189
Models:
Dual Chamber 3326, 3336, 3354
Water Jacketed Incubator
Manual No. 7063158
Important!
Read this instruction manual.

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  • Page 1 P.O. Box 649 Marietta, Ohio 45750 Telephone: (740) 373-4763 Telefax: (740) 373-4189 ________________________________________ Models: Single Chamber 3158, 3164, 3154, 3166 Dual Chamber 3326, 3336, 3354 Water Jacketed Incubator Manual No. 7063158 Important! Read this instruction manual. Failure to read, understand and follow the instructions in this manual may result in damage to the unit, injury to operating personnel and poor equipment performance.
  • Page 2 Forma Scientific, Inc. makes no representations or warranties with respect to this manual. In no event shall Forma Scientific, Inc. be held liable for any damages, direct or incidental, arising out of or related to the use of this manual.
  • Page 3 Forma Scientific, Inc. GENERAL SAFETY NOTES The Occupational Safety and Health Administration (O.S.H.A.) has revised Section 1910-147, The Control Of Hazardous Energy (Lockout/Tagout). Hazardous energy may be: electric, air, hydraulic, water, steam, gravity, spring & all other equally hazardous energy.

  • Page 4: Table Of Contents

    Forma Scientific, Inc. Table of Contents Page Section 1 - Receiving 1.1 Preliminary Inspection ................. 1-1 1.2 Visible Loss or Damage ............... 1-1 1.3 Responsibility for Shipping Damage ............ 1-1 Section 2 - Unpacking List 2.1 Unpacking List..................2-1 Section 3 - Introduction 3.1 The Water Jacket: Key Features ............
  • Page 5 Forma Scientific, Inc. Page 4.12 Connecting the Gas Supply without the Gas Guard ......4-10 4.13 Filling the Humidity Reservoir or Pan..........4-11 4.14 Setting the Chamber Temperature ............4-12 4.15 Setting the Over Temp Safety Thermostat ........... 4-13 4.16 Zeroing the CO 2 Controller..............4-14 4.17 Setting the CO 2 Content ..............
  • Page 6 Forma Scientific, Inc. Page b. Over Temperature Alarm and Pilot Light ........5-9 c. Add Water Pilot Light and Audible Alarm ........5-9 5.7 Remote Alarm Relay System..............5-9 5.8 Recorder Output................... 5-11 Section 6 - Routine Maintenance 6.1 Installing the Decontamination Kit............6-1 6.2 Disinfecting the Incubator Interior ............
  • Page 7 Forma Scientific, Inc. Section 8 - Specifications Section 9 - Auxiliary Equipment Section 10 - Parts List Section 11 - Schematics Section 12 - Warranty Information...

  • Page 8: Section 1 - Receiving

    Inc. ends when the merchandise is loaded onto the carrier's vehicle. On F.O.B. Destination shipments, Forma Scientific's and the carrier's responsibility ends when your Receiving Department personnel sign a free and clear delivery receipt. Whenever possible, Forma Scientific, Inc. will assist in settling claims for loss or in- transit damage.

  • Page 9: Section 2 - Unpacking List

    Forma Scientific, Inc. __________________________________________________________________________ SECTION 2 - UNPACKING LIST 2.1 UNPACKING LIST Remove the packing box from the incubator. If the unit is to be moved by fork lift, leave the incubator on the skid until it has been moved to its designated location. A plastic bag containing the following accessories is packed inside the incubator: Qty.

  • Page 10: Section 3 - Introduction

    _________________________________________________________________________ SECTION 3 - INTRODUCTION 3.1 THE WATER JACKET: KEY FEATURES The Forma Scientific Model 3158 water jacketed incubator is designed to meet the stringent requirements of today's modern laboratory and is a combination of economy, accuracy and reliability. The Model 3158 Incubator features: •...
  • Page 11 Forma Scientific, Inc. _________________________________________________________________________ • No cracks or crevices in, or around, the chamber walls to harbor hidden or hard-to-reach bacterial growth. • Stainless steel shelves, shelf channels and duct sheets which are easily removed without tools for cleaning and disinfecting the entire interior.

  • Page 12: Section 4 - Installation And Start-Up

    Before installing the duct sheets and the shelves, remove the clear plastic film from the shelf brackets and duct sheets. Forma Scientific, Inc. recommends disinfecting all interior surfaces (including both door gaskets). Rinse the surfaces with sterile distilled water (50K Ohm to 1 Meg Ohm).

  • Page 13: Installing The #6 Neoprene Stopper (Access Port)

    Forma Scientific, Inc. ____________________________________________________________________________ 4.3 INSTALLING THE #6 NEOPRENE STOPPER (ACCESS PORT) Open the incubator outer door and inner glass door. Locate the opening in the top left corner of the interior chamber. Place the beveled end of the stopper in the opening.
  • Page 14 Forma Scientific, Inc. ____________________________________________________________________________ Installing the Shelf Brackets and Duct Sheets Figure 4-1...

  • Page 15: Installing The Duct Sheets

    Forma Scientific, Inc. ____________________________________________________________________________ 4.5 INSTALLING THE DUCT SHEETS (Refer to Figure 4-1) Note: The left duct sheet has large notches in both top and bottom edges. The right duct sheet has a single notch in the top edge only.

  • Page 16: Connecting The Incubator To A Power Source

    Refer to the serial tag located on the back of the unit or see Section 8.10 of this manual for specific power requirements. Note: Forma Scientific, Inc. recommends that the incubator be connected to a separate electrical circuit. 4.9 PREPARING THE INCUBATOR FOR FILLING 450 ML of rust inhibitor was placed in the water jacket before the incubator was shipped.
  • Page 17 Forma Scientific, Inc. ____________________________________________________________________________ 4. Turn the incubator power switch ON. The ADD WATER warning light and audible alarm will come on. Pressing the SET/SILENCE button on the alarm panel will silence the audible alarm 5. Remove the adjusting screwdriver from the control panel. The black knob is the handle of the adjusting screwdriver.

  • Page 18: Filling The Water Jacket

    Forma Scientific, Inc. ____________________________________________________________________________ 4.10 FILLING THE WATER JACKET There are two methods of filling the water-jacket. a. Funnel Fill Method b. Tap Fill Method Caution: Purity of the distilled water used in the water jacket and humidifier must be within the 50K ohm to 1 Meg ohm range to protect and prolong the life of the stainless steel water jacket.

  • Page 19: Tap Fill Method

    Forma Scientific, Inc. ____________________________________________________________________________ 2. Attach the funnel into one end of the 3/8" I.D. vinyl tubing. 3. Remove the plastic protective cap from the fill fitting. 4. Attach the free end of the funnel tubing to the fill fitting.

  • Page 20: Connecting The Optional Gas Guard

    The low pressure gauge should have an indicating range of 0 to 30 PSIG to monitor actual input pressure to the incubator injection system. A suitable two-stage pressure regulator is available from Forma Scientific, Inc., Stock #965010. Supply pressure lower than 13.5 PSIG will cause improper operation of the gas guard and may cause the occurrence of nuisance alarms.

  • Page 21: Connecting The Gas Supply Without The Gas Guard

    The low pressure gauge should have an indicating range of 0 to 30 PSIG to monitor actual input pressure to the incubator injection system. A suitable two-stage pressure regulator is available from Forma Scientific, Inc., Stock #965010. 4-10...

  • Page 22: Filling The Humidity Reservoir Or Pan

    Forma Scientific, Inc. ____________________________________________________________________________ Securely attach the gas lines from the supply tank to the desired barbed inlet fitting(s) located on the back of the unit. The fittings are labeled. Refer to Figure 4-6. Check the connections for leaks. Rear view of the control panel, stacked model without Gas Guard Figure 4-6 4.13 FILLING THE HUMIDITY RESERVOIR OR PAN...

  • Page 23: Setting The Chamber Temperature

    Forma Scientific, Inc. ____________________________________________________________________________ a. Reservoir The reservoir in the bottom of the incubator may be filled with at least 3/4" of sterile distilled (50K ohm to 1 Meg ohm) water. b. Humidity Pan The optional humidity pan (Forma Stock #237001) will hold 1.6 gallons (6 liters) of sterile distilled (50K ohm to 1 Meg ohm) water.

  • Page 24: Setting The Over Temp Safety Thermostat

    Forma Scientific, Inc. ____________________________________________________________________________ 4.15 SETTING THE OVER TEMP SAFETY THERMOSTAT (See Figure 4-7) Once the chamber temperature has stabilized (as indicated by the digital display), the Over Temp Safety should be set as follows: 1. Push the SET/SILENCE button on the Alarm Monitor Module.

  • Page 25: Zeroing The Co 2 Controller

    Forma Scientific, Inc. ____________________________________________________________________________ 4.16 ZEROING THE CO 2 CONTROLLER (See Figure 4-8) Important! This adjustment is made using the CO 2 gas content of ambient air (0.03%), the most accurate standard available. Tools Required: Adjustment screwdriver (provided on the panel) FYRITE CO 2 Analyzer (use only for checking) or other CO 2 measuring device.

  • Page 26: Setting The Co 2 Content

    Forma Scientific, Inc. ____________________________________________________________________________ 2. Check the CO 2 level with a FYRITE until two consecutive readings agree. If the FYRITE and display are not within plus or minus 1.0%, consult the factory. Note: After proper zeroing, the CO 2 display will be more accurate than the FYRITE because the zero adjustment was accomplished using absolute.

  • Page 27: Section 5 - Operation

    Forma Scientific, Inc. _________________________________________________________________________ SECTION 5 - OPERATION 5.1 OPERATION The water jacket is filled with approximately 13 gallons (49 liters) of water through the fill fitting located on the front of the unit. The water is then warmed by the chamber heater, providing very stable heating of the incubator chamber.

  • Page 28: Control Panel

    Forma Scientific, Inc. _________________________________________________________________________ When monitoring the effects of CO 2 , absolute humidity must be held constant so any change in thermal conductivity is caused only by a change in the CO 2 concentration. Under the worst circumstances, a change in absolute humidity could cause such a significant change in thermal conductivity that the controller could shift the CO 2 content by as much as 4%.

  • Page 29: Power Switch And Pilot Light

    Forma Scientific, Inc. _________________________________________________________________________ Incubator Control Panel Figure 5-2 b. Power Switch and Pilot Light The main power switch controls the ON/OFF power to the unit. The power pilot light is activated when the power switch is on, and the unit is receiving power.

  • Page 30: Gas Sample Port

    5.4 (FACTORY-INSTALLED OPTION) GAS GUARD STOCK #190410 (Refer to Figure 5-3) Forma Scientific's stock # 190410 built-in Gas Guard is a monitoring and switching device for incubator gas supplies. The unit monitors a two cylinder gas supply. When the active supply tank is depleted,...

  • Page 31: Control Switch

    Forma Scientific, Inc. _________________________________________________________________________ a. Control Switch The CONTROL switch controls power to the gas guard control circuit board. The yellow LEDs on the front panel designate which tank is active and indicate the unit is receiving power. b. Silence Switch The SILENCE switch silences the alarm and selects the alternate tank.

  • Page 32: Pressure Low Indicating Lights

    Forma Scientific, Inc. _________________________________________________________________________ e. Pressure Low Indicating Lights The two red indicating lights, labeled "Pressure Low," indicate when a supply tank is depleted. If both tanks are depleted both red indicating lights will be lit. f. Start-Up Procedure 1. Turn the CONTROL switch on.

  • Page 33: Controller And Digital Display

    Forma Scientific, Inc. _________________________________________________________________________ b. CO 2 Controller and Digital Display The LCD digital readout on the CO 2 module continually displays the percent of CO 2 in the chamber. The setpoint is displayed when the CO 2 SET/SILENCE button is pushed.

  • Page 34: Co Inject Light

    Forma Scientific, Inc. _________________________________________________________________________ Alarm Disable Switch on the CO 2 Control Module Figure 5-5 f. CO 2 Inject Light The CO 2 inject light is activated whenever there is a demand for CO 2 to meet setpoint requirements. Since the CO 2 inject light is independent of the CO 2 alarm, it will continue to signal a need for CO 2 when the CO 2 alarm is set to either the defeat or silence position.

  • Page 35: Add Water Pilot Light And Audible Alarm

    Forma Scientific, Inc. _________________________________________________________________________ b. Over temp Alarm and Pilot Light The over temperature audible alarm and pilot light will come on in the event of an over temp condition. The audible alarm can be silenced for approximately 30 minutes by pressing the SET/SILENCE button.
  • Page 36 When the CONTROL switch on the gas guard is turned off the indicator light will be extinguished and the audible alarm will be silenced. The built-in alarm relay on the Model 3158, however, remains active. A SPDT relay is provided for monitoring alarm conditions on the incubator.

  • Page 37: Recorder Output

    Forma Scientific, Inc. _________________________________________________________________________ Remote Alarm Relay Wiring Diagram Figure 5-7 5.8 RECORDER OUTPUT Incubator temperature and CO 2 information may be directed to a recorder or other data storage device from a four-pin connector located on the back of the control panel.

  • Page 38: Section 6 - Routine Maintenance

    Forma Scientific, Inc. _________________________________________________________________________ SECTION 6 - ROUTINE MAINTENANCE 6.1 INSTALLING THE DECONTAMINATION KIT...

  • Page 41: Disinfecting The Incubator Interior

    Forma Scientific, Inc. _________________________________________________________________________ 6.2 DISINFECTING THE INCUBATOR INTERIOR Use an appropriate disinfectant. All articles and surfaces to be disinfected must be thoroughly cleaned and rough dried. Danger: Alcohol, even a 70% solution, is volatile and flammable. Use it only in a well ventilated area that is free from open flame.

  • Page 42: Cleaning The Cabinet Exterior

    Forma Scientific, Inc. _________________________________________________________________________ 9. Operate incubator for 24 hours to assure removal of trace vapors. Caution: After complete decontamination, it is recommended that the incubator be run and tested before placing any valuable contents inside the incubator chamber. 6.3 CLEANING THE CABINET EXTERIOR The incubator exterior may be cleaned with soap and water and a general use laboratory disinfectant.

  • Page 43: Changing The Co 2 Filter

    Forma Scientific, Inc. _________________________________________________________________________ 6.5 CHANGING THE CO 2 FILTER (For Qualified Service Personnel Only) Note: Under conditions of normal use, and dependent upon the purity of gas being used, the CO 2 filter should be last for about five years.

  • Page 44: Section 7 - Service

    Forma Scientific, Inc. SECTION 7 - SERVICE 7.1 GENERAL TROUBLESHOOTING Symptom Possible Cause • Problem 1: Pilot lights not on. Readouts Unit unplugged, or main power switch not are dark. CO 2 power switch is on. • Circuit breaker tripped.
  • Page 45 Forma Scientific, Inc. Symptom Possible Cause • Problem 7: Digital CO 2 display and Alarm is disabled. • FYRITE read more than 1% different from Defective alarm circuit. • setpoint. CO 2 alarm not on. Defective CO 2 sampler or control.
  • Page 46 Forma Scientific, Inc. Symptom Possible Cause • Problem 12: CO 2 setpoint cannot be Defective CO 2 control. changed. • Problem 13: Unit cannot be zeroed. Defective CO 2 potentiometer. • Defective zero control. • Defective CO 2 Sensor. •...

  • Page 47: Co 2 Control Module Calibration

    7.2 CO 2 CONTROL MODULE CALIBRATION Figure 7-1 Calibration frequency is dependent upon use, environmental conditions, and accuracy required. Forma Scientific recommends a three to four month calibration cycle as a starting point. Before calibrating or making any adjustments to the incubator, it is important that all reference instruments are properly calibrated.

  • Page 48: Control Calibration

    Forma Scientific, Inc. 2. Adjust the CO setpoint to 0% by turning the CO set screw on the control module. (Refer to Figure 7-1) This will prevent CO being injected while the chamber is being calibrated. 3. Turn the CO zero adjust screw on the control panel to match the digital display with the reading of the test instrument.
  • Page 49 Forma Scientific, Inc. 2. Remove the CO2 Control Module by pulling out the black plastic button locking button on the bottom of the module. (Figure 7-3.) 3. Firmly pull the button rearward until the circuit card releases from the internal connector strip.
  • Page 50 Forma Scientific, Inc. 9. Close the doors to the chamber and allow the temperature and humidity to stabilize: - 24 hours if calibrating the unit from a cold start. - 24 hours if the CO sensor has been replaced. - 2 hours if the humidity pan was just filled.
  • Page 51 Forma Scientific, Inc. 13. Allow the chamber to fill and stabilize for at least 15 minutes. 14. Using a Fyrite or other calibrated test instrument, check the CO level in the chamber until two consecutive readings agree. 15. Turn the CO screw to 00.0% to prevent CO...

  • Page 52: Calibrating The Temperature Monitor

    Forma Scientific, Inc. 7.3 37°C CONTROL CALIBRATION If the digital display indicates a temperature other than 37°C when the chamber temperature has stabilized (temperature select switch set to 37°C and it has been at least 45 minutes since the last door opening), it may be necessary to re-calibrate.

  • Page 53: Replacing The Co 2 Sensor

    Forma Scientific, Inc. Extend the control panel out only far enough to access the circuit board and limit activity to the board. 3. Locate the variable resistor identified as “zero” among the circuit board components. The resistor is a long, narrow device with a slotted, brass screw on the top.

  • Page 54: Replacing The Co 2 Solenoid

    Forma Scientific, Inc. 4. Remove the two mounting screws which secure the triac. 5. Carefully disconnect the wires to the triac and note their connections. 6. Remove the triac from the unit. Note the thermal compound between the base of the triac and the floor of the control panel housing. If necessary, reapply more thermal compound before installing the new triac.

  • Page 55: Replacing The Pilot Lights

    Forma Scientific, Inc. 7.9 REPLACING PILOT LIGHTS 1. Loosen the two 1/4-turn fasteners on the front of the control panel and pull the panel out. 2. Disconnect the wiring behind the defective light and break the retaining clip loose. Remove the pilot light.

  • Page 56: Replacing The Blower Motor

    Forma Scientific, Inc. 2. Loosen the 1/4-turn fasteners on the front of the control panel and pull the panel out until the fuse holder in the center of the tray is accessible. 3. Use a fuse puller or a small screwdriver to lift one end of the fuse. Remove the fuse and discard it.

  • Page 57: Stacked Units

    Forma Scientific, Inc. 9. Remove and save the two #6 nuts, washers and nylon spacers that secure the blower motor. Discard the old blower motor. Save the #6 nuts, nylon spacers and washers. 10. Remove the snap ring from the new blower motor shaft.
  • Page 58 Forma Scientific, Inc. 6. Remove the screws securing the base of the front cover plate from the top unit. Let cover plate hang on the left side of the cabinet. 7. Locate and disconnect the electrical connectors to the top unit's floor heater.
  • Page 59 Forma Scientific, Inc. SECTION 8 - SPECIFICATIONS Models 3158/3164 and 3326/3336 Specifications: Models 3158 (3164) Single Chamber 3154 Single Chamber Right hand door swing 3326 (3336) Dual Chamber 3354 Dual Chamber Right Hand Door Swing Temperature ±0.1 o C @ +37 o C (98.6 o F)
  • Page 60 Forma Scientific, Inc. Readability 0.1% Setability 0.1% Alarm Differential and Delay ±1.0% (nominal) for approx. 4 min. Humidity Ambient or 95% RH @ +37 o C (98.6 o F) Humidity Control Humidity Reservoir 1.6 gal. (6 liters) Humidity Pan 1.6 gal. (6 liters) optional...
  • Page 61 Forma Scientific, Inc. Inner Door 1/4" (0.62cm) Fully tempered safety glass with cam action latch Inner Door Gasket Non-porous feather edge silicone Outer Door Gasket Four sided molded magnetic vinyl Finish Powder coated for a durable, easily maintained surface Electrical 3154/3158 90-130 Volts 50/60HZ, 1PH, 4.2 FLA...
  • Page 62 Forma Scientific, Inc. Weights Single Dual 250 lbs. (114 Kg) 500 lbs. (228 Kg) Net Operational 357 lbs. (162 Kg) 714 lbs. (324 Kg) Shipping Motor 280 lbs. (127 Kg) 600 lbs. (272 Kg) 345 lbs. (156 Kg) 700 lbs. (318 Kg) Ocean 400 lbs.
  • Page 63 Forma Scientific, Inc. SECTION 9 - AUXILIARY EQUIPMENT 9.1 AUXILIARY EQUIPMENT 1. Electropolished Stainless Steel Shelf The perforated shelf is square for easy installation. 22 shelf capacity. Catalog # 224140 2. Tissue Culture Shelves These 11" x 16" stainless steel shelves for culture dishes are designed for stacking to increase incubator storage space.
  • Page 64 Forma Scientific, Inc. 6. Two Stage Pressure Regulator Controls CO 2 cylinder gas pressure. First stage reduces tank pressure to pre- set intermediate level. Second stage reduces pressure to recommended incubator inlet pressure. Permits stable CO 2 flow on high or low demand through the entire cylinder supply.
  • Page 65 Forma Scientific, Inc. 11. Hygrometer For measuring chamber humidity. This instrument has a 4" dial and a range of 0 to 100% rh. Catalog # 155010 12. Glass Thermometer For independently measuring chamber temperature. Range from 0°C to 100°C. Catalog # 285722...
  • Page 66 Forma Scientific, Inc. SECTION 10 - PARTS LIST STOCK # DESCRIPTION 770001 CO 2 Disposable Filter 250085 CO 2 Solenoid Valve 12VDC, 3W 300164 Over Temp Relay 431142 Four Sided Molded Magnetic Gasket 400296 Silicon Feather Gasket 230094 Glass Fuse .3A (Slow-Blow Type)
  • Page 67 Forma Scientific, Inc. 10-2...
  • Page 68 Forma Scientific, Inc. 10-3...
  • Page 69 Forma Scientific, Inc. SECTION 11 - SCHEMATICS 11-1...

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