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Summary of Contents for Ecom ECD2800
- Page 1 User’s Guide ECD2800 / ECD2600 UV-VIS DETECTOR ECOM spol. s r.o., Trebonicka 239, 252 19 Chrastany u Prahy, Czech Republic tel: +420 221 511 310, fax: +420 242 498 212, info@ecomsro.cz, http://www.ecomsro.com/ IČO: 411 92 192, DIČ: CZ411 92 192...
- Page 2 ECD2800 / ECD2600 Document properties File: Manual-ECD2000-en-03 Number of pages: File size: 5729 kB Last save: 11/6/2019 8:59:00 AM Dominika Pavlová Ver. Date Author Approved by Description 01.09.2013 Jaroslav Formánek Jaroslav Formánek First issue. 15.04.2015 Jaroslav Formánek Jaroslav Formánek Second issue.
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Page 3: Table Of Contents
ECD2800 / ECD2600 CONTENT: 1. G ....................6 ENERAL INFORMATION 1.1. Product Function ..........................6 1.2. Produced Fersions ..........................6 1.3. Accessories ............................6 2. G ................. 8 ENERAL OPERATING INSTRUCTIONS 2.1. Safety Symbols on Unit ........................8 3. D ........................ - Page 4 14.2. Control of Cell Purity and Absorbing Mobile Phase ..............37 14.3. Replacement of Cell Windows ...................... 38 14.4. Cleaning Cell Windows ........................38 15. T ..................... 39 ECHNICAL ARAMETERS 16. A 1 – ECD2600 CE / ECD2800 CE .... 40 PPENDIX FOR CAPILLARY ELECTROPHORESIS 4 / 47...
- Page 5 ECD2800 / ECD2600 17. A 2 – ECD2600 EX / ECD2800 EX ....... 43 PPENDIX WITH OPTICAL CONNECTORS 18. A 3 – ES D ............46 PPENDIX ECLARATION OF CONFORMITY 5 / 47...
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Page 6: General Information
190 up to 800 nm (600 nm). As a light source is used deuterium lamp combined with tungsten lamp. 1.2. Produced Fersions ECD2800 UV - VIS Detector Cat.: ADA0000X ECD2600 UV - VIS Detector Cat.: ADB0000X... - Page 7 ECD2800 / ECD2600 Picture Description ADA99200 Capillary 0.25 mm 300 mm ECD ADA99400 Hydrodynamic resistor with fittings ADA99900 Waste set ECD2600/2800 00000121 PEEK One-Piece Handtight Nut 1/16", 10-32 ADA40000 Test cell TC01 18329000 Screwdriver Torx T10 99040300 Open End Wrench 7-5.5 mm...
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Page 8: General Operating Instructions
ECD2800 / ECD2600 2. G ENERAL OPERATING INSTRUCTIONS Caution: The unit may not be used, if it is leaking. Caution: Do not place unit or any other equipment so that disconnecting power cord is difficult. Caution: Never manipulate with the unit, if the door is mounted. There is a danger of its damage. -
Page 9: Description
ECD2800 / ECD2600 3. D ESCRIPTION RONT VIEW Description Front switch. Keyboard. Cell compartment. Drain from cell compartment. Leakage sensor. Drain channel with cone for connecting waste system. Door hinges. Slots for tubing and capillaries. Display. Status LED lights: LAMP (green) indicates light sources (deuterium lamp and tungsten lamp). It blinks during lamp turning on/off and lights when a lamp is turned on. - Page 10 ECD2800 / ECD2600 EYBOARD KEYS AND THEIR FUNCTIONS Symbol Description MENU Enter to unit MENU, fast exit from MENU. LAMP Turn on/off light source. Autozero is performed after turned on. Setting the wavelength. ZERO Zeroing. Confirm the entry to the selected MENU item, confirm the new set value of ENTER the parameter, invoking the QUICK DIAGNOSTICS screen.
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Page 11: Installation And Basic Control Using Keyboard
ECD2800 / ECD2600 4. I NSTALLATION AND BASIC CONTROL USING KEYBOARD Place the unit in a suitable location that satisfies the following conditions: • Horizontal place. • Keep at least 10 cm space behind the rear of the unit. •... -
Page 12: Door Installation
ECD2800 / ECD2600 Note: Side and top slots in front panel of the unit are designed for tubing and capillaries accommodation. FEP tubing with outer diameter 1/8” are fitted by pressing into the slot. Capillaries and tubing with outer diameter 1/16” are fitted using short FEP tubing with outer diameter 1/8”... -
Page 13: Basic Control By Keyboard
ECD2800 / ECD2600 4.5. Basic Control by Keyboard After unit start (Mode START) display shows unit type Then the unit turns to mode IDLE, where the lamps are off. After pushing key LAMP the light sources are turning on in mode LAMPS UPDATE, which is... -
Page 14: Description Of Symbols On The Screen
ECD2800 / ECD2600 4.6. Description of Symbols on the Screen 10 Hz *) Sampling rate. 1s *) Time constant for signal filtering. R52% a S53% Light intensity in reference and measuring channel on set wavelength. If S value is significantly lower, it shows dirty cell or absorbing mobile phase. -
Page 15: Parameters
ECD2800 / ECD2600 ENU FLOW DIAGRAM 6.1. Parameters Here can be set basic parameters of the detector. 6.1.1. Wavelength This screen is identical to the screen displayed after pressing key WL. Wavelength can be set in the range of 190-800(600) nm. Keys with arrows left and right move with cursor and up and down change value. -
Page 16: Sample Rate
ECD2800 / ECD2600 Time Constant Peak half width for narrowest expected peek in analysis 1 default Example: narrowest expected peak half width in analysis is 8 s and therefore recommended time constant is 1 s For standard chromatographic analysis is usually suitable default value of time constant 1 s. -
Page 17: Preferences
In each sub menu can be set used lamps, method of their start and eventual preheating. 6.2.1.1. Lamps Use The unit can be set (only model ECD2800) so, that BOTH LAMPS (deuterium and tungsten lamp) are always on, or only deuterium lamp (ONLY DEUTERIUM (D2)) and/or only tungsten lamp (ONLY TUNGSTEN (W)). -
Page 18: Cooling
ECD2800 / ECD2600 OUND SETTING Setting Description KEYBOARD At each touch of button will be generated short beep when the key does not have any significance at the moment, mid- length beep for value change and moving in menu and longer beep for confirmation of value changes. -
Page 19: Opti Filter
ECD2800 / ECD2600 air-conditioned laboratories the best solution (default value is 40 °C). Recommended setting for surrounding temperature under 25 °C is 36 °C, and 40 °C for surrounding temperature over 25°C. Best method to find suitable temperature is to set constant voltage to default value (8 V) and add 2°C to measured temperature after stabilization. -
Page 20: Control
ECD2800 / ECD2600 6.2.8.2. Autozero After WL Change Here autozero after change wavelength in Menu/Parameters/Wavelength. ENABLE is set (Autozero is allowed) or DISABLE is set (Autozero is not allowed). Default setting is ENABLE. Autozero is not performed after change of wavelength with up/down arrows when ENABLE is set. -
Page 21: Remote Keylock
ECD2800 / ECD2600 Connector for ethernet connection is on unit rear side and has two signalization diodes: Location Description LED1 Meaning: OFF = no connection; orange = 10 Mbps; green = 100 Mbps. LED2 Meaning: OFF = no activity; orange = half-duplex mode; green = fully duplex mode. -
Page 22: Interface
ECD2800 / ECD2600 EYLOCK SETTING Setting Description LOCKED Keypad is fully locked. All keys work only for interrupting repetition of fatal error signal. Default value. ALLOW AUTOZERO Keypad is locked except key ZERO. Other keys work only for interrupting repetition of fatal error signal. - Page 23 ECD2800 / ECD2600 6.4.3.1. Analog Input This input serves as a reserve for eventual further customer requests. 6.4.3.2. Digital Input I DIGITAL INPUT may be used as follows: ETTING OF ACTIONS ON DIGITAL INPUTS Setting Description Switch-over to level L does not evoke any action. Default value.
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Page 24: Analog Output
ECD2800 / ECD2600 Setting Description set on OFF. (DIN1) COPY DIN2 If digital input 2 is on level L, output is connected. Even if the digital input is set on OFF. (DIN2) When the leakage sensor is activated, the output is connected. But only... -
Page 25: Info
ECD2800 / ECD2600 Negative dynamic range setting does not influence output range of analogue output. If there is a value −0.8 AU (neg. dynamic range = HIGHER) on the display, on analogue output will be minimal value for selected range. -
Page 26: Lamps
ECD2800 / ECD2600 6.5.5. Lamps Here can be selected lamp we need information about. 6.5.5.1. Deuterium (D2) Displays: NAME - lamp name, IDF- identification number, TIME- operating hours and CHIP- service statistic data from lamp chip. 6.5.5.2. Tungsten (W) Displays: NAME- lamp name and TIME- operating hours 6.5.6. -
Page 27: Cooling
ECD2800 / ECD2600 Name Description Nominal [V] Min [V] Max [V] Positive Analog power supply +11.60 +11.00 +12.00 Negative Analog power supply -11.80 -11.20 -12.20 Positive Digital power supply +5.10 +4.90 +5.30 Positive Low Digital power supply +3.30 +3.20 +3.40 Positive Low Analog power supply +4.80... -
Page 28: Tests
ECD2800 / ECD2600 spectral line. Calibration of wavelength is performed after first initialization of the lamp or manually in Menu/Control/Optical recalibration. 6.6.8. Tests 6.6.8.1. Lamp Energy This item shows value of voltage on reference and measuring diode in volts (REF and SAM Channel) on wavelength with maximum light. - Page 29 ECD2800 / ECD2600 Caution: UV light is harmful for eyes! During regular operation of the detector is UV light perfectly shielded. In case of any service requiring dismantling of any part covering optical unit and when the lamp is on, it is necessary to protect eyes with glasses absorbing UV light.
- Page 30 ECD2800 / ECD2600 If an error is reported during calibration, original values will stay recorded, which may cause that maximal value may differ from 100 % value. Light intensity is the quantity of light incident on reference (REF, prior to cell) or measuring (SAM, after the cell) sensor.
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Page 31: Working Modes
ECD2800 / ECD2600 Disconnect connectors of tungsten lamp on the lamp cable Loosen the screw on the tungsten lamp holder with an open end wrench 5.5mm. Never touch the quartz bulb with bare hands, because fingerprints will irretrievably damage the lamp, Remove the lamp. -
Page 32: Rs232
ECD2800 / ECD2600 ORKING MODES Mode Description EN name Abbreviation Start Transition stage after unit start. Unit is initialized. START Idle Mode during inactive light sources. It is possible to preset operating parameters and make operations not requiring light IDLE source on. -
Page 33: Replacement Of Line Fuse
ECD2800 / ECD2600 9. R EPLACEMENT OF Disconnect the unit from power source!!! Using a flat-tip screwdriver release the fuse holder from its bottom. Take out the fuse holder. Remove old line fuse Insert in the holder new line fuse noted on the product label, which is a part of detector accessories. -
Page 34: Maintenance
ECD2800 / ECD2600 Problem Caused by / Conditions Solution Warning or error message Try to determine the failure according to chapter 6.6. Try to reset the error and if you are not successful, contact the service center. *Higher noise and drift worsen identification and determination of height peak of analyte. This affect the value of the detection limit (LOD) and quantitation limit (LOQ). -
Page 35: Spare Parts
ECD2800 / ECD2600 12. S PARE PARTS Picture Description Spare parts (basic spare parts) ADA33000 Deuterium lamp for ECD2600 and 2800 2052B900 Halogen lamp with cable and connector (only for version 800) ADA40000 Test cell TC01 ADA70000 Analytical cell AC05 (l=5 mm, 1/16", UNF10-32) ADA60000 Analytical cell AC02 (l=2.0 mm, 1/16", UNF10-32) -
Page 36: Warranty And Post -Warranty Repairs
EPAIRS Warranty and post-warranty repairs are provided by the manufacturer or dealer organization authorized by company ECOM to do this. Repair of products in the warranty period carried out by other person than that authorized by the service organization is a reason for warranty nullification. -
Page 37: Order Of Cell Components
ECD2800 / ECD2600 14.1. Order of Cell Components AC05, AC10 ARTS OF ANALYTICAL CELLS 1. Screw M2x4 07608, 2. Cell lid ADA53020, 3. Cell lid seal ADA53030, 4. Cell window 4mm 78000110, 5. Cell body – PEEK. AC02 ARTS OF ANALYTICAL CELL 1. -
Page 38: Replacement Of Cell Windows
ECD2800 / ECD2600 Note the value of light intensity of measuring (S) channel on set wavelength and reference (R) channel. Calculate the % light intensity of S channel to the R channel. If the value % of light intensity of S channel to the R channel is lower than 50 %, the cell may be contaminated, or the mobile phase may be too absorbing. -
Page 39: Technical Parameters
ECD2800 / ECD2600 15. T ECHNICAL ARAMETERS Parameter Value Wavelength range 190-800 nm (190-600 nm) Spectral half-width 6 nm Accuracy of adjustment ± 1 nm Reproducibility ± 0.5 nm Light source Deuterium discharge lamp and tungsten lamp * Noise (Test cell, 254nm, TC 2 s, 10 Hz) ±... -
Page 40: Appendix 1 - Ecd2600 Ce / Ecd2800 Ce For Capillary Electrophoresis
PPENDIX CAPILLARY ELECTROPHORESIS Detectors ECD2600 and ECD2800 are also produced in version for capillary electrophoresis as ECD2600 CE (Cat.No.: ADBCE00X) and ECD2800 CE (Cat. No.: ADACE00X). In this chapter is described installation of capillary and basic unit differences. Description Front switch. - Page 41 ECD2800 / ECD2600 Capillary installation: Please follow the steps described below for capillary installation: Turn off the lamp!!! Open the door on unit front panel. Loosen the screw on black box and move it to left, as shown on the pictures.
- Page 42 ECD2800 / ECD2600 Picture Description 00051478 Terminal block 3 pins green EKAB-040 LAN cable 1 m K0310000 Analog output 18329000 Screwdriver Torx T10 99040300 Open End Wrench 7-5.5 mm 00016000 Door 2000 DOC00001 User’s guide DOC00002 Production protocol 42 / 47...
- Page 43 WITH OPTICAL CONNECTORS Detectors ECD2600 and ECD2800 are also produced in version with external connection of cell using fiber optic cables, as ECD2600 EX (Cat.No.: ADBEX00X) and ECD2800 EX (Cat. No.: ADAEX00X). These detector versions have differences from basic unit as described in this chapter.
- Page 44 ECD2800 / ECD2600 Fiber optic cables installation: Please follow the steps described below for fiber optic cables installation and external cell connection: Before connecting fiber optic cables, remove the connector cover first and unplug the SMA connector of the optical cable.
- Page 45 ECD2800 / ECD2600 diagnostics when working cell is suspected to be polluted or for lamp intensity measuring. Comparing the signal level noise and drift with connected test cell or working cell shows potential problems. When using real fiber optic cables with working cell, the sample signal is a bit lower.
- Page 46 Application of the product: Equipment is used in the lab for measuring of absorbance in liquid by chromatographic analysis. Manufacturer: ECOM spol. s r.o., Trebonicka 239, CZ- 252 19 Chrastany u Prahy, Co. ID No.: 41192192 The following technical standards were applied when evaluating conformity: EN 55011 ed.
- Page 47 ECD2800 / ECD2600 47 / 47...