Related Manuals for CURIOSIS Celloger Mini
Summary of Contents for CURIOSIS Celloger Mini
- Page 1 Automated live cell imaging system Celloger Mini Instruction manual www.curiosis.com...
- Page 2 Developed and manufactured by CURIOSIS Inc. Email: info@curiosis.com Website: www.curiosis.com CURIOSIS Inc. 4F, 10 Teheran-ro 38-gil, Gangnam-gu, Seoul 06221, South Korea Tel: +82-2-508-5237 Fax: +82-2-508-5246 Copyright © 2021, by CURIOSIS Inc. All rights reserved. Published in Korea.
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Page 3: Table Of Contents
Table of contents Package contents Device layout Getting started Installation Network setup Control panel guide Basic operation 1. Device connection 2. Cell monitoring (Setup mode) 3. Details Focusing Manual adjustment Autofocusing Plate panel Features on display Time-lapse imaging 1. Scan position setup (Setup mode) 2. -
Page 4: Package Contents
• Check that all items listed above are included in your package. • Examine the device carefully for any damage during shipping. • Contact your local distributor or info@curiosis.com if any items are missing or damaged. • Any loss or damage claims must be filed with the carrier. -
Page 5: Device Layout
Device layout... -
Page 6: Getting Started
Getting started Installation 1. Remove the product from the box. When taking out the device from the box, ensure to hold the bottom of the device. Brightfield lamp is located at the center of the device and it is not a handle. 2. - Page 7 B. Connect the device to PC using the connection cable and PoE (DATA IN / Left side) 3-2. Connect to power cable (Current version). A. Plug the power cable into PoE B. Connect the device to PC using the connection cable and PoE...
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Page 8: Network Setup
Network setup 1. Click the “Start” button or the “Window” icon. 2. Click the “Setting” button. 3. Click the “Network & Internet” button. 4. Click the “Ethernet” button. 5. Click the “Network and Sharing Center” button. 6. Click the “Change adapter settings” button on the left menu. 7. - Page 9 ⑥ ⑨ ⑧ ⑦...
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Page 10: Control Panel Guide
Control panel guide ① ⑭ ② ⑬ ○ ⑱ ③ ⑨ ③ ② ⑧ ⑩ ④ ⑪ ⑤ ④ ⑫ ⑥ ⑦ ⑭ ⑮ ① APP version : Application version of the software ⑯ ② Start : Device connection ③ Status (Condition or Status) : Preview : Scanning is underway ④... - Page 11 Album mode : Mode to show the files taken with Celloger Mini and video can be made. ✓ Analysis mode : It calculates the confluence of images taken by Celloger Mini and growth curves can be drawn for time-lapse images.
- Page 12 Exchange : Function to make the stage come out of the device for easy mounting of vessel or vessel adapter. ⑯ ✓ Connection status: Showing device connection status ✓ Device IP address : IP address of connected device Device version : Firmware version of connected device ✓...
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Page 13: Basic Operation
Basic operation 1. Device connection 1. Run CellogerMiniApp. 2. After a window pops up, select the icon of the device to be connected in Connect Device and click OK and then the device is connected. ① ② Note : If the device icon is not shown on the window, please check the followings. ①... -
Page 14: Cell Monitoring (Setup Mode)
2. Cell monitoring Positioning (position selection) and light intensity control] 1. Put the device in the incubator. 2. When the stage comes out by pressing , install the vessel. (In case of dishes or flasks, install the vessel adapter first, then the vessel. Refer to Appx B for how to insert a vessel or vessel adapter (page 32). - Page 15 6. Use Jog or Manual position to adjust the position precisely. Rough adjustment is possible with Plate panel (page 17) ① ② ① Jog : Move to the center of the stage. : Move left and right. Using arrow button on the keyboard to move step by step. : Move up and down.
- Page 16 7. Adjust focus manually or using AutoFocus . Autofocus works depending on the AF set range so perform this job after adjusting AF with Jog (page 17). 8. On the Imaging control panel, adjust LED intensity, brightness and contrast by using scroll bar. ✓...
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Page 17: Details
3. Details Focusing ➢ Manual adjustment Adjust focus in Jog by using . Possible to adjust focus by using “ + ”, “ - ” on the number pad of the keyboard. ➢ Auto focusing The Celloger’s Autofocus algorithm scans within a certain depth and selects the area where cells are clearly shown. Therefore, location should be set manually first and execute Autofocusing function. -
Page 18: Features On Display
✓ Is show ‘No’ : If it is checked, scanning order is displayed on the plate panel and if it is not selected, the number disappears. ✓ Is snap to well : After selecting this, it is possible to move from one well to another well using arrow keys on the keyboard. - Page 19 ➢ Zoom, Pan, Capture, Scale bar, Histogram, Center mark, Video area Grid line (Using right-click of the mouse on Display) ✓ Reset Zoom & Pan : Reset after using Zoom, Pan functions ✓ Zoom : Click Ctrl + Mouse wheel to magnify images ✓...
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Page 20: Time-Lapse Imaging
Time-lapse imaging 1. Scan position setup (Setup mode) 1. Place the device into an incubator. 2. Mount vessel when the stages comes to the front after pressing . (Install vessel after installing vessel adapter first in case of dish or flask.) 3. -
Page 21: Time Setup
Time setup 1. Set total imaging time and interval. Minimum interval is 1 minute and total time can be set for up to 60 days. (HH:MM -> Hour:Minute, DD:HH:MM -> Day:Hour:Minute) Check whether storage is enough before starting scanning. Important! It is recommended to set recommended interval time below depending on the number of scan position. -
Page 22: Movie Maker (Album Mode)
➢ How to open the file directly Select the Scan file in the CellogerApp folder or designated folder and go into project name folder. You will find the images saved every cycle by Set position. 4. Movie maker (Album mode) 1. -
Page 23: Image Analysis (Analysis Mode)
Image analysis 1. Confluency (Analysis mode) 1. Select Analysis mode. 2. On Source, select one from Current Image, Local Image, Time-Lapse Images. ✓ Current Image : Image that is shown on display now ✓ Local Image : Select image from images saved in a computer ✓... -
Page 24: Growth Curve
Note : It is possible to see the image of the cycle you want by double clicking in the Time-Lapse Images, but cell mark does not follow. 2. Growth curve 1. Select Analysis mode. 2. On Source, select Time-Lapse Images and open the file from which you want to get the result. 3. -
Page 25: Safety Information
Install the device on a rigid and level places. Operate the device in conditions described in the operating condition. Use only the components provided and authorized by Curiosis. Ensure the input voltage matches with the device’s power supply voltage. ... -
Page 26: Cleaning And Maintenance
Cleaning and Maintenance When cleaning optical elements, use only a damp cloth to avoid scratching soft lens coatings. Lightly wipe working surfaces of the CellogerMini with soft cloth dampened with 70 % ethanol or hydrogen peroxide(H ). Do not pour or spray liquids directly anywhere on the instrument. ... -
Page 27: Trouble Shooting
Trouble shooting Installation Device does not power up ✓ Check power source or contact your distributor. ✓ Replace power cable if it is in poor condition. Cannot connect with CellogerMini device ✓ Make sure the device is connected to the computer. ✓... - Page 28 Reduce interval time or cancel align image setting. (page Others The values in Temperature & Humidity of Celloger Mini are different from those of incubator or separately installed thermometer and hydrometer. ✓ The temperature and humidity sensors of Celloger Mini are not calibrated and used only for the purpose of checking changes in value.
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Page 29: Product Specifications
Accessories PoE adapter, ethernet cable, USB memory Warranty 1 year Ordering information Catalog No. Product Name Description CRCLG-MB01 CELLOGER Mini Live cell imaging system (Bright Field) CRCLG-MBTF25 Vessel holder T-Flask A25cm CRCLG-MBPD35 Vessel holder Petri dish 35mm CRCLG-MBPD60 Vessel holder... -
Page 30: Appendix A. Connecting Multiple Devices
Appendix A. Connecting multiple devices Curiosis assigns the same IP address to Celloger Mini. Therefore, when connecting multiple devices in one PC, device IP change is necessary. In addition, switching hub or router with multiple LAN ports are necessary. PoE Injector... - Page 31 2. Click Device IP and Read to bring up the Device IP. 3. Change the IP Address to 192.168.2.## and press Change. Put the numbers from 2 to 254 except for 10 in ##. <Example> Note : If connection with PC is not possible because Device IP address is lost, there is no way to know the IP address.
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Page 32: Appendix B. How To Insert Vessel Or Vessel Holder
Appendix B. How to insert Vessel or Vessel Holder Select the adapter suitable for the vessel type. As shown in the figure below, tilt and insert the adapter or well plate. At this time, insert the area where it contacts with a ball plunger and insert it on the other side. Area where it contacts with a ball plunger Adapter or... -
Page 33: Appendix C. Correct Focus And Light Intensity Selection
Appendix C. Correct focus and light intensity selection <1> When it is in focus: Most of the cells look clearly. Raw image Image with cell mark <2> Out of focus: Some cells are difficult to see as they are separated from the background and are not visible. Raw image Image with cell mark <3>... - Page 34 Curiosis Inc. HEADQUARTERS 4F, 10 Teheran-ro 38-gil Gangnam-gu, Seoul 06221 South Korea TEL +82-2-508-5236 FAX +82-2-508-5246 Email: info@curiosis.com FACTORY 400 Wonam-ro, Namsa-myeon Cheoin-gu, Yongin-si, Gyeonggi-do 17123 South Korea TEL +82-31-339-6404 FAX +82-31-339-6409 www.curiosis.com...
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