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Summary of Contents for CURIOSIS Celloger Mini
- Page 1 Automatic Live Cell Imaging System Quick Manual...
- Page 2 Table of contents Device Layout Basic operation 1) Installation 2) Set up 3) Starting Description 1) Set up mode 2) Scan mode 3) Album mode 4) Analysis mode Operation 1) Set up 2) Vessel Setting & Focusing 3) Image Scan & Time lapse image capture 4) Video recording 5) Confluency &...
- Page 3 Device Layout...
- Page 4 Front Layout Bright field lamp X-Axis Motor Temperature and humidity sensor Objective Lens Vessel holder X-Y Stage...
- Page 5 Rear Layout Side Layout Bright field lamp Vessel holder X-Axis Motor X-Axis Motor X-Y Stage LAN & Power cable connector LAN & Power cable connector...
- Page 6 Basic Operation...
- Page 7 1. Installation LAN & Power Cable Connect the device to PC Step 1. Remove the product from the box. Step 2. Plug the power cable into device Step 3. Connect the device to PC using the connection cable.
- Page 8 2. Set up Cell seeding Step 1. Prepare a sample. Step 2. Put the device inside incubator. Step 3. Place the sample on the stage. Note: Check the connection between the PC and the device. ü Whole procedure should be done in clean area to avoid to decrease accuracy of captured imaging due to dust. ü...
- Page 9 3. Starting Click! Step 1. Click a Celloger Mini App Step 2. Select the file to save and Connect device Step 3. Press Start streaming button to display the current plate. Note: If the device icon does not appear, enter the IP address directly.
- Page 10 Description...
- Page 11 1. Set up mode Current X-Y-Z position Live cell monitoring Current temperature & humidity Connection with Celloger Mini X-Y-Z Control panel Selectable resolution • Speed • Normal • Step[mm] • High • Set position Move to absolute X-Y-Z position in mm unit...
- Page 12 1. Set up mode Vessel Type Image saving formats Autofocusing Minimum / maximum range for autofocusing Auto ROI : The clearest place among the 9 areas is selected automatically Used to exchange the plate Device Light up...
- Page 13 2. Scan mode X-Y-Z position Start & Stop & Pause Current progress of the work Schedule • Period • Total time • Total cycle Current Image location information Refresh Scale bar Display...
- Page 14 3. Album mode Captured image play Video speed control Stop Video recording Import file X-Y position Captured image history Measure cell length Cell detection Light Scale bar Refresh...
- Page 15 4. Analysis mode Import image Confluency Adjust Color settings Display Scale bar...
- Page 16 Operation...
- Page 17 1. Set up mode Imaging set intensity Camera brightness Camera contrast Autofocusing Frame Resolution Result Step 1. Press Start streaming button, then image is displayed. Step 2. To adjust illumination, use the LED intensity, camera brightness, camera contrast on the imaging tap.
- Page 18 2. Vessel Setting & Focusing Step 1. Select the well plate of the Vessel type and the options such as image saving format. Step 2. Specify where to take the picture. Step 3. Adjust the focus using the autofocusing button or jog tap. Step 4.
- Page 19 3. Image Scan & Time lapse image capture Step 1. Press the scan mode. Step 2. Select a file to save the captured image. Step 3. Set up total time and period in the schedule, the total cycle is entered automatically. Step 4.
- Page 20 ü Select the name of image in the data list. The preview window will show the image and movie you selected. ü Warm up Celloger Mini for 1 hour in incubator before making a movie. If not, it might be the cause of uncertain data or unclear videos.
- Page 21 5. Confluency & Growth Curve confluency & growth curve Cell line : HeLa 30.9% Step 1. Press the analysis mode Step 2. Import an image or folder Select Live image (1image) / Local image (1image) / Time-Lapse images (folder) in source menu Step 3.
- Page 22 6. Additional setting Measure cell length Other settings < Grid line > < Histogram > < Center mark > Step 1. Click the right button of the mouse on display Step 2. Click “Histogram” or “Center mark” or “Measure” or “Grid line” Note : Grid line can be set according to user's convenience (default : 3 x 3)
- Page 23 Copyright ã2020, by CURIOSIS, Inc. All rights reserved. Published in Korea. Revision History: V.0.0 Date: Dec 2019 V.0.1 Date: Feb 2020...
- Page 24 4F 10, Teheran-ro 38-gil, Gangnam-gu, Seoul 06221, South Korea Curiosis Inc. T: 82 2 508 5236 F: 82 2 508 5246 www.curiosis.co.kr...
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