Buchi Lyovapor L-300 Operation Manual
  1. Manuals
  2. Brands
  3. Buchi Manuals
  4. Dryer
  5. Lyovapor L-300
  6. Operation manual

Buchi Lyovapor L-300 Operation Manual

Hide thumbs Also See for Lyovapor L-300:
Table of Contents

Advertisement

Quick Links

Download this manual
Lyovapor™ L-300/L-300 Pro
Operation Manual

Advertisement

Table of Contents
loading

Related Manuals for Buchi Lyovapor L-300

Summary of Contents for Buchi Lyovapor L-300

  • Page 1 Lyovapor™ L-300/L-300 Pro Operation Manual...
  • Page 2 CH-9230 Flawil 1 E-Mail: quality@buchi.com BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experi- ence, especially with respect to structure, illustrations and technical details. This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for com- petitive purposes, nor made available to third parties.

  • Page 3: Table Of Contents

    BÜCHI Labortechnik AG Table of contents Table of contents About this document...................... 7 Connected devices .......................... 7 Warning notices in this document...................... 7 Symbols ...............................  7 1.3.1 Warning symbols ........................ 7 1.3.2 Mark-ups and symbols...................... 8 Trademarks............................ 8 Safety............................ 9 Proper use ............................ 9 Use other than that intended .......................
  • Page 4 Requirements for local network settings ................ 32 5.4.2 Preparing the instrument for app using..................  33 5.4.3 Enabling BUCHI Cloud access .................... 33 Insert SD card (Pro control panel only).................... 33 Operating the control panel.................... 35 Layout of the control panel ........................  35 Function bar............................
  • Page 5 BÜCHI Labortechnik AG Table of contents Operating Pro control panel ..................... 47 Layout of Pro control panel........................  47 Function bar............................ 47 Other symbols on the control panel .................... 49 Menu bar............................ 49 7.4.1 Start menu .......................... 49 7.4.2 Favourites menu ........................ 50 7.4.3 Method menu .........................
  • Page 6 Table of contents BÜCHI Labortechnik AG Cleaning and servicing ..................... 88 Regular maintenance work ........................  88 Performing a vacuum test........................ 89 Performing a leak test........................ 90 9.3.1 Performing a leak test with a drying chamber................  90 9.3.2 Performing leak test with a manifold drying rack .............. 92 Cleaning the ice condenser .......................

  • Page 7: About This Document

    If you have any questions after reading this operation manual: Contact BÜCHI Labortechnik AG Customer Service. https://www.buchi.com/contact Connected devices In addition to these operating instructions, you should also follow the instructions and specifications in the documentation for the connected devices.

  • Page 8: Mark-Ups And Symbols

    1 | About this document BÜCHI Labortechnik AG Symbol Meaning Explosive substances Dangerous electrical voltage Flammable substances Device damage 1.3.2 Mark-ups and symbols NOTE This symbol draws attention to useful and important information. R This character draws attention to a requirement that must be met before the in- structions below are carried out.

  • Page 9: Safety

    BÜCHI Labortechnik AG Safety | 2 Safety Proper use The Lyovapor™ L-300 is used for freeze-drying solid materials in ampoules, vials, trays, round-neck or wide-neck flasks and is exclusively intended for that purpose. The Lyovapor™ L-300 can be used in laboratories for the following tasks: —...

  • Page 10: Location Of Warning Signs On The Product

    — Safety-related incidents that occur while using the device should be reported to the manufacturer (quality@buchi.com). BUCHI service technicians Service technicians authorized by BUCHI have attended special training courses and are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair measures.

  • Page 11: Location Of Warning Signs On The Ice Condenser

    BÜCHI Labortechnik AG Safety | 2 Fig. 2: Location of warning signs on the product General warning Dangerous electrical voltage Flammable substances 2.4.3 Location of warning signs on the ice condenser Fig. 3: Location of warning signs on the product Hand injuries Hot surface Residual risks The device has been developed and manufactured using the latest technological ad-...

  • Page 12: Faults During Operation

    Technical modifications to the device or accessories should only be carried out with the prior written approval of BÜCHI Labortechnik AG and only by authorized BUCHI technicians. BUCHI accepts no liability whatsoever for damage arising as a result of unauthorized modifications. 12/106...

  • Page 13: Product Description

    BÜCHI Labortechnik AG Product description | 3 Product description Description of function The Lyovapor™ is a freeze-dryer in which frozen samples can be gently dried. The basis of freeze-drying is sublimation. Sublimation refers to the process whereby a substance transforms directly from the solid to the gaseous state. The physical process of sublimation can be explained using the solvent water as an example.

  • Page 14: Secondary-Drying Phase

    3 | Product description BÜCHI Labortechnik AG Controlling the heat transfer prevents the following critical temperatures for amor- phous and crystalline materials being reached: — the glass transition temperature T ' of the frozen sample. — the collapse temperature T —...
  • Page 15 BÜCHI Labortechnik AG Product description | 3 Fig. 4: Front View Connections for heated shelf Intermediate valve (Lyovapor™ L-300 Pro only) Ice condenser Optional mounting position for control panel Ventilation slots Castors Castor brake On/Off master switch User interface Operation Manual Lyovapor™ L-300/L-300 Pro 15/106...

  • Page 16: Rear View

    3 | Product description BÜCHI Labortechnik AG 3.2.2 Rear view Fig. 5: Rear view Power isolating switch Type plate/Refrigerant specifications Connections for pressure sensors Side connections (option) Connection for fresh water Connection for condensate drain hose I Connection for condensate drain Ventilation slots hose II Gas connection for venting valve...

  • Page 17: Control Panel

    (option) (option) Automatic stoppering connection Connection for fresh water sensor Connection for receiving vessel sen- Connection for external pressure sen- sors (optional) LAN port Standard BUCHI communication port (COM) 3.2.4 Control panel Fig. 7: Control panel View Function buttons Navigation control Operation Manual Lyovapor™...

  • Page 18: Pro Control Panel

    Fig. 8: Pro control panel Touch-screen display Navigation control Type plate The type plate identifies the instrument. The type plate is located at the rear of the in- strument. Buchi Operations India Private Limited 394230 Surat / India Type xxxxxxxx Volt xxx-xxx...

  • Page 19: Scope Of Delivery

    BÜCHI Labortechnik AG Product description | 3 Scope of delivery NOTE The scope of delivery depends of the configuration of the purchase order. Accessories are delivered as per the purchase order, order confirmation, and deliv- ery note. Refrigerant Specification 3.5.1 Refrigerant Specification (Low temperature) The refrigerant details are shown on the back of the instrument.

  • Page 20: Lyovapor™ L-300

    3 | Product description BÜCHI Labortechnik AG Specification L-300 for 50Hz L-300 for 60Hz Weight 272 kg 272 kg Minimum clearance on all sides 400 mm 400 mm Power supply 380 – 400 V 3N~ 208 – 220 V 3~ Power consumption 6000 VA 5000 VA (maximum)

  • Page 21: Ambient Conditions

    BÜCHI Labortechnik AG Product description | 3 Specification L-300 for 50Hz L-300 for 60Hz Vacuum generation time to 0.1 Typically ≤ 15 min Typically ≤ 15 min mbar* Volume-based leakage rate* Typically ≤ 0.001 Typically ≤ 0.001 mbar x L/sec mbar x L/sec Minimum system vacuum* Typically ≤...

  • Page 22: Transport And Storage

    4 | Transport and storage BÜCHI Labortechnik AG Transport and storage Transport NOTICE Risk of breakage due to incorrect transportation Make sure that the instrument is fully dismantled. Pack every instrument components properly to prevent breakage. Use the original packaging whenever possible. Avoid sharp movements during transit.

  • Page 23: Installation

    BÜCHI Labortechnik AG Installation | 5 Installation Installation site The installation site must meet the following requirements: DANGER Risk of explosion from flammable gas-and-air mixtures The potential consequences are death or extremely serious injury. Do not damage the refrigerant circuit piping. Store and operate the instrument in a room with a volume of at least 16.6 m that an ignitable gas-and-air mixture cannot develop.

  • Page 24: Putting The Instrument In Operation

    5 | Installation BÜCHI Labortechnik AG Fig. 12: Castor brake NOTE Make sure that the power supply can be disconnected at any time in an emergency. NOTE Depending on the ambient conditions, condensation may form on any cold surface on the unit. Putting the instrument in operation NOTICE Instrument damaged if restarted too early...

  • Page 25: Fitting The Fresh Water Connections

    BÜCHI Labortechnik AG Installation | 5 3. Be equipped with suitable fuses and electrical safety features. 4. Be equipped with a proper earthing. Make sure that all connected devices are earthed. Make sure that the power plug is freely accessible at all times. Insert the power plug into the power socket.

  • Page 26: Fitting Fill-Level Sensor In Water Tank (Optional)

    5 | Installation BÜCHI Labortechnik AG Fit the fresh water level sensor (optional). See Chapter 5.2.4 "Fitting fill-level sen- sor in water tank (optional)", page 26 5.2.4 Fitting fill-level sensor in water tank (optional) Fig. 14: Fitting the fresh water level sensor Switch the On/Off master switch to Off. Fit the sensor on the fresh water container above the inlet tube.

  • Page 27: Fitting The Fill-Level Sensor On The Receiving Vessel (Optional)

    BÜCHI Labortechnik AG Installation | 5 Fig. 15: Fitting the drain tube Side connections Drain tube See Chapter 3.2.3 "Side connec- tions", page 17 Connection for condensate drain tube Switch the On/Off master switch to Off. Fit the drain tube (4) onto the connection marked Drain 1 (5). Fix the drain tube in place with a hose clip.

  • Page 28: Connecting The Coolant

    5 | Installation BÜCHI Labortechnik AG 5.2.7 Connecting the coolant Fig. 17: Connecting the coolant Coolant inlet connection Coolant outlet connection Drain tube Inlet tube Precondition: R The coolant connection complies with the specified parameters. See Chapter 3.6 "Technical data", page 19 Make sure that the instrument is not connected to the power supply. Fit the inlet tube (4) onto the coolant inlet connection (1).
  • Page 29 BÜCHI Labortechnik AG Installation | 5 Fig. 18: Fitting the pressure sensors Pressure sensor Pressure sensor Clamp, ISO-KF 16 Clamp, ISO-KF 16 Seal, ISO-KF 16 Connection for pressure sensor Seal, ISO-KF 16 Connection for pressure sensor Connection 10 Connection Fitting pressure sensor one Switch the On/Off master switch to Off.

  • Page 30: Connecting Inert Gas (Option)

    5 | Installation BÜCHI Labortechnik AG 5.2.9 Connecting inert gas (option) Fig. 19: Connecting inert gas Inert gas tube Tube clip Gas connection for pressure regulat- Gas connection for venting valve ing valve Tube clip Inert gas tube Connecting the inert gas to the pressure regulating valve Precondition: R The gas connection complies with the specified parameters.

  • Page 31: Fitting An Air Filter (Option)

    BÜCHI Labortechnik AG Installation | 5 5.2.10 Fitting an air filter (option) Fig. 20: Fitting an air filter Air filter Tube clip Tubing Tube clip Gas connection for pressure regulat- Gas connection for venting valve ing valve Tube clip Tubing Tube clip 10 Air filter Fitting an air filter for the pressure regulating valve Precondition:...

  • Page 32: Establishing Lan Connection

    5 | Installation BÜCHI Labortechnik AG NOTICE Closed gas ballast valve when using solvent. A closed gas ballast valve when using solvents can cause damage to the instrument. When using solvents, keep the gas ballast valve open. NOTE To increase the service life of the vacuum pump, operate the vacuum pump with an open gas ballast valve.

  • Page 33: Preparing The Instrument For App Using

    BÜCHI Labortechnik AG Installation | 5 In order to use the BUCHI Cloud a DNS server must be configured on the instru- ment. NOTE If there is no DNS server available enter the IP address for the BUCHI Cloud connec- tion manually.
  • Page 34 5 | Installation BÜCHI Labortechnik AG Fold the control panel forward. Insert the SD card on the underside. Switch on the instrument. ð The status bar shows the SD card symbol. The following data is stored on the SD card: —...

  • Page 35: Operating The Control Panel

    BÜCHI Labortechnik AG Operating the control panel | 6 Operating the control panel This section describes the operation of the instrument using the control panel. CAUTION Risk of injury from glass splinters Sharp objects can damage the display. Keep sharp objects away from the display. Layout of the control panel Fig. 22: Layout of the control panel Description...

  • Page 36: Menu Bar

    6 | Operating the control panel BÜCHI Labortechnik AG General function buttons Symbol Description Meaning [Back] The display reverts to the previous view. [Cancel] Cancels an operation. E SC [Add to favourites] Adds the selected item to the [[Favourites]] menu. [Remove from Removes the selected item from the favourites]...

  • Page 37: Start Menu

    BÜCHI Labortechnik AG Operating the control panel | 6 Menu sym- Meaning Submenu/Action — Process control parameters [Start] menu — Bookmarks for individual entry points [Favourites] menu — Process settings [Configuration] menu — Settings — Servicing — Service — System information —...
  • Page 38 Action Option Explanation [Mobile connection View The control panel shows a password password] for entry in the BUCHI Monitor app. [Mobile connection View The control unit shows a QR code QR code] for reading by the BUCHI Monitor app. [Language]...
  • Page 39 Display illumination level in %: 0 - [Network] Enter value The following parameters can be edited: Device name/MAC address/DHCP/ System IP address/Subnet mask/ Gateway/DNS server/BUCHI Cloud/ Server IP address [Delete app con- Confirmation question Resets external connections to the nection] instrument.
  • Page 40 6 | Operating the control panel BÜCHI Labortechnik AG Action Option Explanation [Refrigerant circuit] View The following information on the re- frigerant circuit is available: — Duty hours — Compressor high temperature — Compressor low temperature — Inlet temperature, ice condenser 1 —...
  • Page 41 BÜCHI Labortechnik AG Operating the control panel | 6 Action Option Explanation [Vacuum system] View The following information on the vac- uum system is available: — Pump hours of duty — Pump oil hours of duty — Pressure, ice condenser 1 —...
  • Page 42 6 | Operating the control panel BÜCHI Labortechnik AG Action Option Explanation [[Stoppering Sys- View The following information on the tem]] stoppering system is available: — Number of uses — Start — On — Off — Oil pressure Submenu System Information The submenu [[System information]] contains details of the connected components and information on network connection diagnosis.

  • Page 43: Status Bar

    BÜCHI Labortechnik AG Operating the control panel | 6 Submenu Notifications The submenu [Notifications] shows a list of unacknowledged and unresolved notifica- tions together with date and time in each case. Submenu Logbook The submenu [Logbook] shows the instrument's message history. Logbook: —...

  • Page 44: Carrying Out Freeze-Drying

    The current parameters of the freeze-drying process are being re-established. Symbols on the status bar Symbol Status The instrument is connected to the BUCHI Cloud. The instrument is defrosting. The instrument is starting up. The instrument is in energy-saving mode. Before the freeze-drying process: Load the top-mount drying rack with a frozen sample.

  • Page 45: Starting Freeze-Drying

    BÜCHI Labortechnik AG Operating the control panel | 6 Tap the function [[Start conditioning]] on the function bar. ð The temperature in the ice condenser cools down to operating temperature. ð The vacuum pump is being brought up to operating temperature. ð...

  • Page 46: Shutting Down The Instrument

    6 | Operating the control panel BÜCHI Labortechnik AG Precondition: R The sample is dry. Navigate to the [Start] menu via the navigation path. Tap the function [Aerate] on the function bar. Answer YES to the confirmation question. ð The system is vented. ð...

  • Page 47: Operating Pro Control Panel

    BÜCHI Labortechnik AG Operating Pro control panel | 7 Operating Pro control panel This section describes the operation of the instrument using the Pro control panel. CAUTION Risk of injury from glass splinters Sharp objects can damage the display. Keep sharp objects away from the display. Layout of Pro control panel Fig. 23: Layout of Pro control panel Description...
  • Page 48 7 | Operating Pro control panel BÜCHI Labortechnik AG Symbol Description Meaning [Cancel] Cancels an operation. E SC [Add to favourites] Adds the selected item to the [[Favourites]] menu. [Remove from Removes the selected item from the favourites] [Favourites] menu. [Confirm] Confirms an entry.

  • Page 49: Other Symbols On The Control Panel

    BÜCHI Labortechnik AG Operating Pro control panel | 7 Symbol Description Meaning [Skip] Skips vacuum pump warm-up SKIP phase. The other conditioning phase pro- cesses are carried out. [[Deactivate sample Manually deactivates sample protec- protection]] tion. [Copy] Copies the selected method. C OP Y [Open] Opens the selected valve.
  • Page 50 7 | Operating Pro control panel BÜCHI Labortechnik AG Setting parameters using the navigation control Select a parameter by turning the navigation control. ð The control panel highlights the selected parameter in green. Tap the function [Edit] on the function bar. ð...
  • Page 51 Action Option Explanation [Mobile connection View The control panel shows a password password] for entry in the BUCHI Monitor app. [Mobile connection View The control unit shows a QR code QR code] for reading by the BUCHI Monitor app. [Language]...
  • Page 52 Display illumination level in %: 0 - [Network] Enter value The following parameters can be edited: Device name/MAC address/DHCP/ System IP address/Subnet mask/ Gateway/DNS server/BUCHI Cloud/ Server IP address [Delete app con- Confirmation question Resets external connections to the nection] instrument.
  • Page 53 BÜCHI Labortechnik AG Operating Pro control panel | 7 Action Option Explanation [Refrigerant circuit] View The following information on the re- frigerant circuit is available: — Duty hours — Compressor high temperature — Compressor low temperature — Inlet temperature, ice condenser 1 —...
  • Page 54 7 | Operating Pro control panel BÜCHI Labortechnik AG Action Option Explanation [Vacuum system] View The following information on the vac- uum pump is available: — Pump hours of duty — Pump oil hours of duty — Pressure in the ice condenser —...

  • Page 55: Messages Menu

    BÜCHI Labortechnik AG Operating Pro control panel | 7 Submenu System Information The submenu [[System information]] contains details of the connected devices and in- formation on network connection diagnosis. Action Option Explanation [Control panel] View The following information on the control panel is available: —...

  • Page 56: Status Bar

    7 | Operating Pro control panel BÜCHI Labortechnik AG Symbol Description Meaning Acknowledged The message has been be processed and acknowledged. < Sent The message initiator is no longer present. > Received The display shows a message. Status bar The status bar shows the status of the instrument. The following statuses are possible: Indication on status bar View...

  • Page 57: Editing A Method

    The instrument is starting up. The instrument is in the course of a manual freeze-drying process. The instrument is connected to the BUCHI Cloud. Sample protection is active. Reason: the pressure is outside the pressure lim- its.

  • Page 58: Creating A New Method

    7 | Operating Pro control panel BÜCHI Labortechnik AG Creating a new method Navigation path ➔ Method Navigate to the [Method] menu via the navigation path. Tap the function [New] on the function bar. ð The new method is created. Creating a new method by copying an existing method Navigation path ➔...

  • Page 59: Setting The Gas Type

    BÜCHI Labortechnik AG Operating Pro control panel | 7 Tap the function [Save] on the function bar. ð The setting is saved. ð The dialog box closes. 7.6.4 Setting the gas type Navigation path ➔ Method Navigate to the [Method] menu via the navigation path. Tap the name of the method that you wish to edit.
  • Page 60 7 | Operating Pro control panel BÜCHI Labortechnik AG Navigate to the [Method] menu via the navigation path. Tap the name of the method that you wish to edit. ð The control panel highlights the selected method in green. Tap the action [Steps]. ð...

  • Page 61: Setting The Phases Of A Method

    BÜCHI Labortechnik AG Operating Pro control panel | 7 When asked to confirm, press [OK]. ð The step is deleted. 7.6.7 Setting the phases of a method NOTE The settings in the Phase view affect all steps of a phase. Navigation path ➔...
  • Page 62 7 | Operating Pro control panel BÜCHI Labortechnik AG Phase Setting Option Meaning [[Sec- [[Pressure ac- None/Sample None: no action is carried out. ondary tion]] protection/ Sample protection: if the pressure is drying]] Message too high, heating of the shelves is paused.

  • Page 63: Deleting A Method

    BÜCHI Labortechnik AG Operating Pro control panel | 7 Tap the function [Save] on the function bar. ð The setting is changed. Deleting a method Navigation path ➔ Method Navigate to the [Method] menu via the navigation path. Tap the name of the method that you wish to edit. ð...

  • Page 64: Temperature Difference Test

    7 | Operating Pro control panel BÜCHI Labortechnik AG Setting Option Explanation [[Start time]] Enter value Sets the time from which the pressure difference test is to be performed. The value relates to the time before completion of the primary drying phase or the secondary drying phase.

  • Page 65: Pressure Rise Test

    BÜCHI Labortechnik AG Operating Pro control panel | 7 Tap the name of the method that you wish to edit. ð The control panel highlights the selected method in green. Tap the action [Phase]. ð The control panel shows the action Phase. Tap the setting [End point definition].
  • Page 66 7 | Operating Pro control panel BÜCHI Labortechnik AG Navigate to the [[Method]] menu via the navigation path. Tap the name of the method that you wish to edit. ð The control panel highlights the selected method in green. Tap the action [[Phase]]. ð...

  • Page 67: Performing Freeze-Drying Using A Method [Pro Control Panel]

    BÜCHI Labortechnik AG Operating Pro control panel | 7 Setting Option Explanation [[Message]] Yes/No The control panel shows or does not show a message as soon as the temperature difference test is passed. Performing freeze-drying using a method [Pro control panel] 7.9.1 Preparing the instrument Time...

  • Page 68: Changing Parameters While The Process Is Running

    7 | Operating Pro control panel BÜCHI Labortechnik AG Navigation path ➔ Start If a gas is being used Precondition: R The instrument has been prepared. R A method is selected. Fit a top-mount drying rack. See Chapter 8 "Operating top-mount drying racks", page 73.

  • Page 69: Shutting Down The Instrument

    BÜCHI Labortechnik AG Operating Pro control panel | 7 Remove the dried preparation from the top-mount drying rack. 7.9.6 Shutting down the instrument NOTICE Not completed shut down of the instrument. An interruption while shutting down the instrument can damage the instrument. Shut down the instrument completely.

  • Page 70: Starting Freeze-Drying

    7 | Operating Pro control panel BÜCHI Labortechnik AG Navigate to the [Start] menu via the navigation path. Tap the function [[Start conditioning]] on the function bar. ð The temperature in the ice condenser cools down to operating temperature. ð The vacuum pump is being brought up to operating temperature. ð...

  • Page 71: End Point Definition

    BÜCHI Labortechnik AG Operating Pro control panel | 7 Tap the function [Edit] on the function bar. ð The control panel shows a dialog box with a numeric input box. ð The control panel highlights the selected parameter in white. Enter the value in the numeric input box.

  • Page 72: Switching Off The Instrument

    7 | Operating Pro control panel BÜCHI Labortechnik AG Time 80 min quired: Navigation path ➔ Start Precondition: R The freeze-drying process has ended. R The gas balast on the vacuum pump is open. R Make sure that there is sufficient distilled water available. Navigate to the [Start] menu via the navigation path.

  • Page 73: Operating Top-Mount Drying Racks

    BÜCHI Labortechnik AG Operating top-mount drying racks | 8 Operating top-mount drying racks Operating stoppering acrylic drying chamber Make sure that the groove above the ice con- denser is clean, free of dust and not scratched. Check the O-ring for damage and grease it with vacuum grease.
  • Page 74 8 | Operating top-mount drying racks BÜCHI Labortechnik AG Loosen all fixing screws. Align the slots for the shelves. Tighten the fixing screws. Place the rack on the intermediate plate. Slide the shelves into the rack. 74/106 Operation Manual Lyovapor™ L-300/L-300 Pro...
  • Page 75 BÜCHI Labortechnik AG Operating top-mount drying racks | 8 Press the plug onto the shelf connection and turn the ring counterclockwise at the same time. (option) Turn the temperature sensor electrical con- nector so that the marks on the connector and the heatable shelf are parallel Press the temperature sensor electrical con- nector onto the connection.

  • Page 76: Operating Manifold Acrylic Drying Chamber (Heatable Shelves)

    8 | Operating top-mount drying racks BÜCHI Labortechnik AG Precondition: R The status bar shows the status Stoppering. Turn the hand wheel, until all samples are sealed. Confirm the verification question on the control panel. Operating manifold acrylic drying chamber (heatable shelves) Make sure that the groove above the ice con- denser is clean, free of dust and not scratched.
  • Page 77 BÜCHI Labortechnik AG Operating top-mount drying racks | 8 Loosen all fixing screws. Align the slots for the shelves. Tighten the fixing screws. Place the rack on the intermediate plate. Slide the shelves into the rack. Operation Manual Lyovapor™ L-300/L-300 Pro 77/106...
  • Page 78 8 | Operating top-mount drying racks BÜCHI Labortechnik AG Press the plug onto the shelf connection and turn the ring counterclockwise at the same time. (option) Turn the temperature sensor electrical con- nector so that the marks on the connector and the heatable shelf are parallel Press the temperature sensor electrical con- nector onto the connection.

  • Page 79: Operating Manifold Acrylic Drying Chamber (Non-Heatable Shelf)

    BÜCHI Labortechnik AG Operating top-mount drying racks | 8 Carry out freeze-drying. Operating manifold acrylic drying chamber (non-heatable shelf) Make sure that the groove above the ice con- denser is clean, free of dust and not scratched. Check the O-ring for damage and grease it with vacuum grease.
  • Page 80 8 | Operating top-mount drying racks BÜCHI Labortechnik AG Place the rack on the intermediate plate. Locate the cylinder in the groove above the main plate. Fit manifold valve onto the connection on the top-mount drying rack. Check the O-ring for damage and grease it with vacuum grease.

  • Page 81: Operating Acrylic Drying Chamber (Heatable Shelf)

    BÜCHI Labortechnik AG Operating top-mount drying racks | 8 Carry out freeze-drying. Operating acrylic drying chamber (heatable shelf) Make sure that the groove above the ice con- denser is clean, free of dust and not scratched. Check the O-ring for damage and grease it with vacuum grease.
  • Page 82 8 | Operating top-mount drying racks BÜCHI Labortechnik AG Loosen all fixing screws. Align the slots for the shelves. Tighten the fixing screws. Place the rack on the intermediate plate. Slide the shelves into the rack. 82/106 Operation Manual Lyovapor™ L-300/L-300 Pro...

  • Page 83: Operating Acrylic Drying Chamber (Non-Heatable Shelf)

    BÜCHI Labortechnik AG Operating top-mount drying racks | 8 Press the plug onto the shelf connection and turn the ring counterclockwise at the same time. (option) Turn the temperature sensor electrical con- nector so that the marks on the connector and the heatable shelf are parallel Press the temperature sensor electrical con- nector onto the connection.
  • Page 84 8 | Operating top-mount drying racks BÜCHI Labortechnik AG Place the main plate on the ice condenser. Make sure that the groove on the ice con- denser is clean, free of dust and not scratched. Check the 300 mm dia. O-ring for damage and grease it with vacuum grease.

  • Page 85: Operating Manifold Drying Rack

    BÜCHI Labortechnik AG Operating top-mount drying racks | 8 Check the O-ring for damage and grease it with vacuum grease. Place the O-ring into the groove of the cover. Place the cover on the cylinder. Carry out freeze-drying. Operating manifold drying rack Make sure that the groove above the ice con- denser is clean, free of dust and not scratched.

  • Page 86: Determining The Number Of Flasks

    8 | Operating top-mount drying racks BÜCHI Labortechnik AG Place the base plate on the main plate. Place the seal on the connection. Fit the manifold drying rack onto the seal and fix it with the clamp. Carry out freeze-drying. Determining the number of flasks NOTE Do not overfill flasks...

  • Page 87: Operating Manifold Valves

    BÜCHI Labortechnik AG Operating top-mount drying racks | 8 Flask volume 1000 mL Flask volume 500 mL Rotating freezing Max. 22 flasks Max. 22 flasks Static freezing Max. 36 flasks Max. 36 flasks Operating manifold valves The lever on a manifold valve can be set to the following positions: Position Function A: Lever pointing up...

  • Page 88: Cleaning And Servicing

    Users may only carry out the servicing and cleaning operations described in this sec- tion. Any servicing and repair work which involves opening up the casing may only be car- ried out by BUCHI service technicians. Regular maintenance work Component...

  • Page 89: Performing A Vacuum Test

    BÜCHI Labortechnik AG Cleaning and servicing | 9 Component Action Interval View Wipe down the display with a damp Monthly cloth. Ice condenser Clean the ice condenser. See Chap- Annually ter 9.4 "Cleaning the ice condenser", page 93 Performing a vacuum test The vacuum test checks the performance capacity of the vacuum system.

  • Page 90: Performing A Leak Test

    9 | Cleaning and servicing BÜCHI Labortechnik AG Possible cause Action The pump connected is not delivering Carry out vacuum test with a different sufficient performance vacuum pump. Performing a leak test 9.3.1 Performing a leak test with a drying chamber The leak test checks the vacuum system for possible leaks.
  • Page 91 BÜCHI Labortechnik AG Cleaning and servicing | 9 Ice condenser 2 24.4L 47.2L 54.1L Acrylic drying chamber Acrylic drying chamber (with 4 drying shelves) (with 6 drying shelves) Both ice condensers 33.5L 56.3L 63.2L Acrylic drying chamber Acrylic drying chamber (with 4 drying shelves) (with 6 drying shelves) Tap the function [Start] on the function bar.

  • Page 92: Performing Leak Test With A Manifold Drying Rack

    9 | Cleaning and servicing BÜCHI Labortechnik AG Possible cause Action Pump oil dirty Service according to manufacturer's in- structions. Leaking manifold valves Replace leaking manifold valve. (manifold acrylic drying chamber only) Locate leaking manifold valve. See Chapter 10.3 "Locating leaking manifold valve", page 96 9.3.2 Performing leak test with a manifold drying rack...

  • Page 93: Cleaning The Ice Condenser

    BÜCHI Labortechnik AG Cleaning and servicing | 9 ð The leak test is passed if the measured leakage rate is less than the pre-set rate of 10.10 mbar*L/h. NOTE To test the leak-tightness of the manifold drying rack, carry out another leak test with the manifold drying rack installed.
  • Page 94 9 | Cleaning and servicing BÜCHI Labortechnik AG Tap the action [Drain valve 1]. ð The control panel shows the drain valve highlighted in green. Tap the function [Open] on the function bar. Tap the action [Drain valve 2]. ð The control panel shows the drain valve highlighted in green. Tap the function [Open] on the function bar.

  • Page 95: Help With Faults

    Drain valve is leaking. Check whether closed drain tube contracts. If it does, contact BUCHI Ser- vice. Instrument is not The connection for the gas Make sure that gas is able vented supply is blocked.

  • Page 96: Locating Leaking Manifold Valve

    10 | Help with faults BÜCHI Labortechnik AG 10.3 Locating leaking manifold valve Navigation path ➔ Start Precondition: R The system does not evacuate to less than 0.1 mbar. Navigate to the Start view via the navigation path. Set the vacuum to 0.5 mbar. Tap the function [Start] on the function bar.

  • Page 97: Taking Out Of Service And Disposal

    Dispose of the appliance properly, if necessary using a professional disposal ser- vice. When disposing of equipment observe the local regulations and statutory require- ments regarding waste disposal. 11.2 Returning the instrument Before returning the instrument, contact the BÜCHI Labortechnik AG Service Depart- ment. https://www.buchi.com/contact Operation Manual Lyovapor™ L-300/L-300 Pro 97/106...

  • Page 98: Appendix

    Appendix 12.1 Spare parts and accessories Use only genuine BUCHI consumables and spare parts in order to ensure correct, safe and reliable operation of the system. NOTE Any modifications of spare parts or assemblies are only allowed with the prior written permission of BUCHI.

  • Page 99: Spare Parts

    BÜCHI Labortechnik AG Appendix | 12 Description Order no. Image Air filter 11057925 SD-Card 1 GB 11064730 Connection cable, for vacuum pump 11064934 12.1.3 Spare parts Description Order no. Image User interface 11063580 User interface Pro 11063581 O-Ring Ø 300 mm 11065367 O-Ring Ø...
  • Page 100 12 | Appendix BÜCHI Labortechnik AG Description Order no. Image Vacuum clamp KF 16 11064939 Vacuum clamp KF 25 11063662 Vacuum clamp, KF 40 11063663 Vacuum seal KF 16 11063455 Vacuum seal KF 25 11063457 Vacuum seal KF 40 11063659 O-Ring, Silicone, 94.61 x 6.99 mm 11062420 O-Ring, Silicone, 161.3 x 5.33 mm...

  • Page 101: Top-Mount Drying Rack Accessories

    BÜCHI Labortechnik AG Appendix | 12 Description Order no. Image Blind flange KF 16 11064902 Blind flange KF 25 11063660 Blind flange KF 40 11063661 12.1.4 Top-mount drying rack accessories Description Order no. Image Drying chamber tube, PMMA, for 4 11063278 shelves L 368 mm, Ø...
  • Page 102 12 | Appendix BÜCHI Labortechnik AG Description Order no. Image Rack for 4 heatable shelves 11065102 H 356 mm, Ø 265 mm , shelf distance 30-75 Rack for 6 heatable shelves 11065103 H 468 mm, Ø 265 mm, shelf distance 30-75 Heatable shelf, aluminum coated, with 11064095 connection cable...
  • Page 103 BÜCHI Labortechnik AG Appendix | 12 Description Order no. Image Baseplate, PMMA, for manifold rack 11065733 Suction nipple 11065819 For manifold application to create vacuum in sample flasks Ampoule adapter for manifold 11065725 with 19 ampoule connections and cap adapter Manifold valve, natural ruber, with SJ 11062300 29/32...

  • Page 104: Software

    12 | Appendix BÜCHI Labortechnik AG Description Order no. Image Manifold flask adapter set 11066144 with 12 adapters, incl. filter paper Manifold flask adapter set 11067334 with 6 adapters, incl. filter paper Manifold flask adapter set, US joint size 11066171 with 12 adapters, incl.
  • Page 106 T +971 4 313 2860 T +52 55 9001 5386 F +66 2 862 08 54 F +971 4 313 2861 latinoamerica@buchi.com bacc@buchi.com middleeast@buchi.com www.buchi.com/es-es www.buchi.com/th-th www.buchi.com We are represented by more than 100 distribution partners worldwide. Find your local representative at: www.buchi.com...

This manual is also suitable for:

Lyovapor l-300 pro

Table of Contents