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Summary of Contents for biochrom WPA Lightwave 3
- Page 1 Lightwave 3 & Lightwave 3+ Spectrophotometers Users Manual 5061-084-REV-1...
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Page 2: Table Of Contents
TABLE OF CONTENTS WARRANTY AND REPAIR Warranty Policy SAFETY INFORMATION Hazards and Warnings INTRODUCTION The Biochrom WPA Spectrophotometers INSTALLATION Unpacking Positioning Installing INSTRUMENT OVERVIEW Scope Spectrophotometer Principle and Intended Use Hardware Technical Specifications Display and Keypad Instrument Connections PVC PC Software... - Page 3 TABLE OF CONTENTS Kinetics Standard Curve Multi Wavelength Absorbance Ratio Favourites, Methods, and USB Memory Stick Settings Date and Time Regional Printer Preferences Contrast About the Instrument Additional Options Status Bar Icons USEFUL CALCULATIONS Beer-Lambert Law TROUBLESHOOTING BUILT-IN PRINTER Built-in Printer Accessory Part Numbers Printer Installation Guide Refilling the Printer Paper ORDERING INFORMATION...
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Page 4: Warranty And Repair
Warranty Policy Goods will not be accepted for return unless the RMA (Return Materials Authorization) number has been IN NO EVENT SHALL BIOCHROM BE LIABLE issued. The unit must be returned with the completed FOR INCIDENTAL OR CONSQUENTIAL DAMAGES RMA forms and the Decontamination checklist. The... -
Page 5: Safety Information
SAFETY INFORMATION Hazards and Warnings • Use, store and dispose of any chemicals in accordance with manufacturer’s guidelines This section describes potential hazards which may and local safety regulations. exist in the operation of these units. A number of • Provide suitable ventilation when working warning labels and symbols are affixed to your with volatile solvents or toxic substances. -
Page 6: Introduction
INTRODUCTION Biochrom WPA Spectrophotometers Spectrophotometers are ubiquitous among modern laboratories. Ultraviolet (UV) and Visible (VIS) spectrophotometry has become the method of choice in most laboratories concerned with the identification and quantification of organic and inorganic compounds across a wide range of products and processes. -
Page 7: Installation
INSTALLATION Unpacking • The unit weighs less than 4 kg. No special handling is required. • Please keep the original packaging for transport for service or repair as it has been specifically designed to protect the unit from damage during transit. •... -
Page 8: Instrument Overview
80-3007-42 WPA Lightwave 3 80-3007-43 WPA Lightwave 3 with Printer 80-3007-44 WPA Lightwave 3 with Bluetooth 80-3007-45 WPA Lightwave 3 with Printer and Bluetooth 80-3007-47 WPA Lightwave 3+ 80-3007-48 WPA Lightwave 3+ with Printer 80-3007-49 WPA Lightwave 3+ with Bluetooth... -
Page 9: Technical Specifications
INSTRUMENT OVERVIEW Technical Specifications Wavelength Range 190 to 1100 nm Monochromator Flat grating Wavelength Calibration Automatic upon switch on Beam Height 15 mm Spectral Bandwidth 5 nm or 3 nm for ‘+’ models Wavelength Accuracy ±2 nm Wavelength Reproducibility ±1 nm Light Sources Xenon flash lamp Detector... -
Page 10: Pvc Pc Software
(.rtf), tab-separated value (.tsv) or in a native PVC format file. Biochrom Resolution PC Software When connected to a PC the spectrophotometers can be controlled using the Biochrom Resolution PC software packages. Operation using Biochrom Resolution PC software is described in the Resolution user manual or Resolution help file. -
Page 11: User Interface
USER INTERFACE Keypad The instrument is controlled using the snap-dome switch-keys of the membrane keypad, offering tactile feedback during operation. Instrument Firmware The instrument firmware uses an intuitive menu arrangement that is navigated using the membrane keypad. Upon powering on the instrument, and after completion of the internal calibration, the home screen is displayed. LIGHTWAVE 3 &... -
Page 12: Home Screen
USER INTERFACE Home Screen The instrument home screen is the first screen displayed after initialisation of the instrument, and all applications and settings can be accessed from here. Home screen for the Home screen for the Lightwave 3 spectrophotom- Lightwave 3 spectrophotometer eter displaying the USB memory Stick application, made available when a USB Flash drive is inserted Applications... -
Page 13: Single Wavelength
USER INTERFACE Single Wavelength The Single Wavelength application is accessed from the Applications screen using the ‘1’ key. It can be used to performs simple absorbance (A) or % transmission (%T) measurements. Step 1 Set the wavelength, 190 to 1100 nm, using the left and right arrow or alphanumeric keys. -
Page 14: Concentration
USER INTERFACE Step 6 Replace the previous sample with a test sample then take a sample measurement using the confirm key. Repeat for all samples. WARNING! Previous measurements cannot be recalled so should be printed or output to a USB flash drive before any subsequent samples are measured. - Page 15 USER INTERFACE Step 3 Press the down arrow, enter up to 8-digits to define the units that the measurements will be reported in. Open the Units option using the options key: Select one of the predefined units; “µg/ml”, “µg/µl”, “pmol/µl”, “mg/dl”, “mmol/l”, “µmol/l”, “g/l”, “mg/l”, “µg/l”, “U/l”, “%”, “ppm”, “ppb”, or “conc”, using the left and right arrow keys.
- Page 16 USER INTERFACE Step 6 Insert the reference sample then take a reference measurement using the reference key. The acquired reference sample baseline will be applied to all subsequent sample measurements. Step 7 For “Standard” mode, replace the previous sample with a standard sample then take a standard measurement using the confirm key.
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Page 17: Wavescan
USER INTERFACE Step 8 Replace the previous sample with a test sample then take a sample measurement using the confirm key. Repeat for all samples. WARNING! Previous measurements cannot be recalled so should be printed or output to a USB flash drive before any subsequent samples are measured. - Page 18 USER INTERFACE Step 5 Proceed to the measurement screen by selecting “OK” using the confirm key. Return to the Applications screen by selecting “Cancel” using the escape key. Step 6 Insert the reference sample then take a reference measurement using the reference key. The acquired reference sample baseline will be applied to all subsequent sample measurements.
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Page 19: Kinetics
USER INTERFACE Kinetics The Kinetics application is accessed from the Applications screen using the ‘4’ key. It can be used to perform a series of absorbance (A) measurements over a defined timeframe creating a time-course trace. Step 1 Set the wavelength, 190 to 1100 nm, using the left and right arrow or alphanumeric keys. - Page 20 USER INTERFACE Step 7 Press the down arrow, enter up to 8-digits to define the units that the final result will be reported in. Open the units options using the options key: Select one of the predefined units; “µg/ml”, “µg/µl”, “pmol/µl”, “mg/dl”, “mmol/l”, “µmol/l”, “g/l”, “mg/l”, “µg/l”, “U/l”, “%”, “ppm”, “ppb”, or “conc”, using the left and right arrow keys.
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Page 21: Standard Curve
USER INTERFACE Step 11 Insert the reference sample then take a reference measurement using the reference key. The acquired reference sample baseline will be applied to all subsequent sample measurements. Step 12 Replace the previous sample with a test sample then take a sample measurement using the confirm key. - Page 22 USER INTERFACE Step 1 Set the wavelength, 190 to 1100 nm, using the left and right arrow or alphanumeric keys. Step 2 Press the down arrow, set the number of standard samples of known concentration, 1 to 9, using the left and right arrow or alphanumeric keys.
- Page 23 USER INTERFACE Step 5 Proceed to the second parameters screen by selecting “Next” using the confirm key. Return to the Applications screen by selecting “Cancel” using the escape key. Step 6 Select the curve fit, “Zero Regression”, “2nd Order Polynomial”, “Regression”, “Interpolation”, or “Cubic Spline”, using the left and right arrow keys.
- Page 24 USER INTERFACE Step 10 Proceed to the Calibration screen by selecting “Next” using the confirm key. Return to the previous parameters screen by selecting “Back” using the escape key. Step 11 Insert the reference sample then take a reference measurement using the reference key. The acquired reference sample baseline will be applied to all subsequent standard and test sample measurements.
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Page 25: Multi Wavelength
USER INTERFACE Step 14 Insert a test sample then take a sample measurement using the confirm key. Repeat for all samples. WARNING! Previous measurements cannot be recalled so should be printed or output to a USB flash drive before any subsequent samples are measured. Results The results are displayed on the screen. - Page 26 USER INTERFACE Step 3 Proceed to the second parameters screen by selecting “OK” using the confirm key. Return to the Applications screen by selecting “Cancel” using the escape key. Step 4 Press the down arrow, set the sample prompt, “Yes” or “No”, to bring up the sample number option before each measurement, using the left and right arrow keys.
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Page 27: Absorbance Ratio
USER INTERFACE Step 7 Replace the previous sample with a test sample then take a sample measurement using the confirm key. Repeat for all samples. WARNING! Previous measurements cannot be recalled so should be printed or output to a USB flash drive before any subsequent samples are measured. - Page 28 USER INTERFACE Step 4 Proceed to the parameter screen by selecting “OK” using the confirm key. Return to the Applications screen by selecting “Cancel” using the escape key. Step 5 Enter any dilution factor to be applied to the absorbance measurement of a value of up to four significant figures, using the alphanumeric keys.
- Page 29 USER INTERFACE Step 8 Proceed to the measurement screen by selecting “OK” using the confirm key. Return to the wavelengths screen by selecting “Back” using the escape key. Step 9 Insert the reference sample then take a reference measurement using the reference key. The acquired reference sample baseline will be applied to all subsequent sample measurements.
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Page 30: Favourites, Methods, And Usb Memory Stick
USER INTERFACE Favourites, Methods, and USB Memory Stick The Favourites, Methods, and USB Memory Stick screens are accessed from the home screen using the ‘5’, ‘6’, or ‘8’ key respectively. They are directories to save custom methods to, using the options menu from the results screen (see the “Additional Options”... - Page 31 USER INTERFACE 3. To lock protect a saved custom method application, use the options key then press the ‘2’ key. 4. To unlock a protected saved custom method application, use the options key then press the ‘3’ key. 5. To back up or restore custom method applications, insert a USB flash drive then use the options key then press the ‘4’...
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Page 32: Settings
USER INTERFACE Settings The Settings screen is accessed from the home screen using the ‘7’ key. It can be used to access application to adjust the instrument settings, such as date, time, language, and number format. Default Settings screen displaying Settings screen with the Games application hidden the Games application Date and Time... -
Page 33: Regional
USER INTERFACE Regional The Regional application is accessed from the Settings screen using the ‘2’ key. It can be used to change the language and decimal separator number format. Step 1 Set the language using the left and right arrow keys. Step 2 Press the down arrow, set the number format decimal separator using the left and right arrow keys. -
Page 34: Preferences
USER INTERFACE Preferences The Preference application is accessed from the Settings screen using the ‘4’ key. It can be used to set the access to the games application, the menu layout theme, parameter history, and standby settings. Step 1 Set access to the games application or not, using the left and right arrow keys. -
Page 35: Additional Options
USER INTERFACE About The About pop-up window is opened from the Settings screen using the ‘6’ key. The pop-up window displays information about the instrument, including its serial number, firmware version, and build. Step 1 Set the brightness using the left and right arrow keys. Step 2 Press the down arrow, set the contrast using the left and right arrow keys. - Page 36 USER INTERFACE Print Graph – Print graph via selected setting. Run Standard – Re-run and standard sample measurement. Peak Detection – Open the peak detection parameters to define the auto-peak detection settings. Step 1 Set the method to auto-detect peaks or not using the left and right arrow keys.
- Page 37 USER INTERFACE Graph Scale – Open the graph scale parameters Step 1 Set the zoom mode using the left and right arrow keys. Step 2 Press the down arrow, apply x axis limits or not using the left and right arrow keys. For “On”...
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Page 38: Status Bar Icons
USER INTERFACE Sample Number… Open save method options. Selection the folder location in which to save the method using the left and right arrow keys. Press the down arrow, Insert up to a 25-digit method name using the alphanumeric keys. Save the method and return to the measurement screen by selecting OK using the confirm key. -
Page 39: Useful Calculations
USEFUL CALCULATIONS Beer-Lambert Law A = cεl A is the absorbance, which although unit-less is usually described as A or AU (absorbance units). c is the concentration in molar units (M). ε is the molar extinction coefficient in per molar unit per cm (M-1cm-1). l is the pathlength in centimetres (cm). -
Page 40: Troubleshooting
TROUBLESHOOTING Negative absorbance • Sample measurements will be negative absorbance reading if the absorbance value of the reference is higher than the sample. readings • Negative readings can also result if reference and sample are interchanged or if the sample is very dilute and close to the absorbance of the reference. Unexpected results •... -
Page 41: Built-In Printer
BUILT-IN PRINTER Built-in Printer Accessory Part Numbers 80-3003-84 – Built-in Printer accessory 80-3004-07 – Spare paper for printer (20 rolls) Printer Installation Guide Step 1 Invert the instrument and remove the screws from positions A and B. Step 2 Return the instrument to its upright position and lift the accessory cover vertically upwards. -
Page 42: Refilling The Printer Paper
BUILT-IN PRINTER Step 4 Lower the printer onto the locating bosses and push down firmly. Step 5 Invert the instrument and replace the accessory cover screws at A and B. Refilling the Printer Paper Step 1 Lift off the printer cover using the tabs and discard the spent roll. -
Page 43: Ordering Information
80-3007-42 WPA Lightwave 3 80-3007-43 WPA Lightwave 3 with Printer 80-3007-44 WPA Lightwave 3 with Bluetooth 80-3007-45 WPA Lightwave 3 with Printer and Bluetooth 80-3007-47 WPA Lightwave 3+ WPA Lightwave 3+ with Printer 80-3007-48 WPA Lightwave 3+ with Bluetooth 80-3007-49... - Page 44 Contact Us Biochrom Ltd. Biochrom U.S. Harvard Bioscience (Shanghai) Co., Ltd. Building 1020, Cambourne Business Park 84 October Hill Road Room 8C, Zhongxi Tower, 121 Jiangsu Road, Cambourne, Cambridge, UK, CB23 6DW Holliston, Massachusetts 01746 USA Changning District, Shanghai, China, 200050...
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