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Summary of Contents for biochrom Libra S6
- Page 1 Libra S6 User Manual English Deutsch Français Español Italiano...
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Page 2: Declaration Of Conformity
This is to certify that the Libra S6 and S6H Visible Spectrophotometers Part number 80-5000-10 and 80-5000-11 Serial number 88000 onwards manufactured by Biochrom Ltd. conform to the requirements of the following Directives-: 73/23/EEC & 89/336/EEC Standards to which conformity is declared... -
Page 3: Table Of Contents
CONTENTS Unpacking, Positioning and Installation Essential Safety Notes OPERATION Introduction Using the Instrument Absorbance and % Transmission Absorbance Ratio Cell Density Scan Factor Concentration Standard Curve Kinetics To recall a saved method SET UP ACCESSORIES ERROR MESSAGES OUTPUT OF RESULTS Use with serial printer Use with chart recorder USE WITH PC AND THE GRAFICO PC UTILITY... -
Page 5: Unpacking, Positioning And Installation
Before commencing installation, please take time to familiarise yourself with these symbols and their meaning. Caution (refer to accompanying documents). Background colour yellow, symbol and outline black. ___________________________________________________________________ Issue 01 - 12/2004 Libra S6, English... -
Page 6: Operation
• enables export of the output from the instrument to Excel as a text file A tutorial on UV/Visible spectrophotometry is included as part of the Grafico software. ___________________________________________________________________ Libra S6, English Issue 01 – 12/2004... -
Page 7: Using The Instrument
Note that the light beam shines from LEFT to RIGHT through the cell chamber; ensure the cell is inserted in the correct alignment. The optical height is 15mm, and the minimum volume that can be used is approx. 700µl in a semi-micro cell. ___________________________________________________________________ Issue 01 - 12/2004 Libra S6, English... -
Page 8: Absorbance And % Transmission
F1, then 56 Select second wavelength Repeat as necessary All OK Insert reference 0A/100%T Used for subsequent samples until RT on display changed Insert sample Absorbance values are displayed Repeat as necessary To exit ___________________________________________________________________ Libra S6, English Issue 01 – 12/2004... -
Page 9: Absorbance Ratio
Insert reference 0A/100%T Used for subsequent samples until RT on display changed Insert sample Value is displayed; an auto- correction factor is applied to the Absorbance value. Repeat as necessary To exit ___________________________________________________________________ Issue 01 - 12/2004 Libra S6, English... -
Page 10: Scan
To zoom in on a region of interest: Zoom F2, then Move box that appears on display to area 3456 of interest Zoom in Examine detail Zoom out Return to original data To exit ___________________________________________________________________ Libra S6, English Issue 01 – 12/2004... -
Page 11: Factor Concentration
Insert sample Concentration is displayed Repeat as necessary To delete F3, then F1 To exit Note: It is not necessary to enter the name, and this can be omitted for a quick measurement. ___________________________________________________________________ Issue 01 - 12/2004 Libra S6, English... -
Page 12: Standard Curve
Absorbance for Std 1 is measured Std 2 is highlighted Insert Standard 2 Absorbance for Std 2 is measured Std 3 is highlighted Repeat as necessary Incorrect entry? F3, then F1, Clears entry prior to re-entry ___________________________________________________________________ Libra S6, English Issue 01 – 12/2004... - Page 13 0A/100%T Used for subsequent samples until RT on display changed Insert sample Concentration is displayed Repeat as necessary To delete F3, then F1 To exit To delete F2, then F1 To exit ___________________________________________________________________ Issue 01 - 12/2004 Libra S6, English...
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Page 14: Kinetics
F4, then Usually 00m 00s, unless there is a lag Enter Start Time 3456 time Accept Interval is highlighted F4, then Minimum interval is 10 seconds Enter time interval between 3456 each measurement ___________________________________________________________________ Libra S6, English Issue 01 – 12/2004... -
Page 15: To Recall A Saved Method
Press Comment Make a measurement Select a method Select method number Accept Selected method is recalled Run method Insert reference Used for subsequent samples until 0A/100%T RT on display changed Insert sample ___________________________________________________________________ Issue 01 - 12/2004 Libra S6, English... -
Page 16: Set Up
Heater control* May not be available* Heated cell Select on for thermostatting at 37°C Accept To exit *Heated cell holder factory fitted version only. This option cannot be fitted retrospectively to an instrument. ___________________________________________________________________ Libra S6, English Issue 01 – 12/2004... -
Page 17: Accessories
(1 red, 1 black) 4mm banana plugs at the other (depending on the chart recorder) and 2 metres of coaxial cable or screened twin core, with the shield connecting the black plug and pin 5 and the core connecting the red plug and pin 1. ___________________________________________________________________ Issue 01 - 12/2004 Libra S6, English... -
Page 18: Use With Pc And The Grafico Pc Utility Software
(by dragging the icon at the left side of the displayed graph and releasing at the appropriate point). Graphs can be copied and pasted into Word, Excel or powerpoint Graphs can be saved in a format that can be opened directly by Excel ___________________________________________________________________ Libra S6, English Issue 01 – 12/2004... -
Page 19: Menu Descriptions
Toolbar View menu bar as icons Status bar View status bar at bottom of display Help Tutorial View tutorial on UV/Visible spectrophotometry Help topics View help topics About View version number etc ___________________________________________________________________ Issue 01 - 12/2004 Libra S6, English... -
Page 20: Practical Aspects
Select chart type “XY Scatter” and the curved lines (no data points) option • Label the axes etc as required • Double click on the x-axis, select Scale and minimum to 330 and maximum to • Set colour scheme to suit your preferences ___________________________________________________________________ Libra S6, English Issue 01 – 12/2004... -
Page 21: Maintenance
Do not touch the optical surfaces of the lamp with your fingers (use tissue); if touched, the area should be cleaned with iso-propanol. • Instructions for lamp change are provided with the lamp and overleaf. ___________________________________________________________________ Issue 01 - 12/2004 Libra S6, English... -
Page 22: Cleaning And General Care Of The Instrument
3. Place the instrument on its back, insert a small flat headed screwdriver into the potentiometer slot and turn it right or left until a suitable level of brightness is obtained. ___________________________________________________________________ Libra S6, English Issue 01 – 12/2004... -
Page 23: Specification And Warranty
This product has been designed and manufactured by Biochrom Ltd, 22 Cambridge Science Park, Milton Road, Cambridge CB4 0FJ, UK.
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