Table of Contents
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Table of Contents
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Summary of Contents for biochrom Libra S4
- Page 1 Libra S4 User Manual English Deutsch Français Español Italiano...
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Page 2: Declaration Of Conformity
This is to certify that the Libra S4 Visible Spectrophotometer Part number 80-5000-00 Serial number 88000 onwards manufactured by Biochrom Ltd. conform to the requirements of the following Directives-: 73/23/EEC & 89/336/EEC Standards to which conformity is declared EN 61 010-1: 2001 Safety requirements for electrical equipment for measurement, control and laboratory use. -
Page 3: Table Of Contents
CONTENTS Unpacking, Positioning and Installation Essential Safety Notes OPERATION Introduction Sample handling tips Using the Instrument Absorbance and % Transmission Concentration Rate Factor (time and date) Use with serial printer Use with chart recorder USE WITH PC AND THE GRAFICO PC UTILITY SOFTWARE Installation Introduction... -
Page 5: Unpacking, Positioning And Installation
Before commencing installation, please take time to familiarise yourself with these symbols and their meaning. Caution (refer to accompanying documents). Background colour yellow, symbol and outline black. ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 6: Operation
Note that test tubes do not last forever, and that the surface gets scratches and blemishes through repetitive use; if this is the case they should be replaced. ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 7: Using The Instrument
Error messages may appear on the display and mean the following: FAIL flashing Can carry on using; refer to error messages section FAIL constant Cannot use; refer to error messages section and contact your supplier ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 8: Absorbance And % Transmission
Insert sample to measure sample Concentration relative to standard is displayed Repeat as necessary To set a factor manually for use in concentration measurements, go to Factor mode (see later in manual). ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... - Page 9 Measures standard and entered concentration is displayed (factor is calculated). Wavelength appears when measurement finished Insert sample to measure sample Concentration relative to standard is displayed Repeat as necessary ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English...
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Page 10: Rate
Keeps measuring until the key is pressed or until 1000 measurements are made Repeat as necessary Note that there is no t = 0 reading; the first readings are those after 10 seconds. ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 11: Factor
Moves to Concentration mode Insert reference Used for subsequent samples until 0A/100%T to set reference changed Insert sample to measure sample Concentration calculated from factor and absorbance is displayed Repeat as necessary ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 12: (Time And Date)
(1 red, 1 black) 4mm banana plugs at the other (depending on the chart recorder) and 2 metres of coaxial cable or screened twin core, with the shield connecting the black plug and pin 5 and the core connecting the red plug and pin 1. ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 13: Use With Pc And The Grafico Pc Utility Software
(by dragging the icon at the left side of the displayed graph and releasing at the appropriate point). Graphs can be copied and pasted into Word, Excel or powerpoint Graphs can be saved in a format that can be opened directly by Excel ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 14: Menu Descriptions
Toolbar View menu bar as icons Status bar View status bar at bottom of display Help Tutorial View tutorial on UV/Visible spectrophotometry Help topics View help topics About View version number etc ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 15: Practical Aspects
Select chart type “XY Scatter” and the curved lines (no data points) option • Label the axes etc as required • Double click on the x-axis, select Scale and minimum to 330 and maximum to • Set colour scheme to suit your preferences ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 16: Accessories
Pixel clock too high, contact your local distributor Constant Pixel clock too low, contact your local distributor Constant Pixel clock unstable, contact your local distributor Constant PDA failure, contact your local distributor ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 17: Maintenance
• Note that as well removal for cleaning, spillages in the cell holder can be flushed through using water from a squeeze bottle in order to prevent crystallisation / fermentation of residues. ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 18: Lamp Replacement
3. Place the instrument on its back, insert a small flat headed screwdriver into the potentiometer slot and turn it right or left until a suitable level of brightness is obtained. ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 19: Student Experiments
(preferably sealed). Other equipment Beakers or conical flasks for distilled water, wash bottle and supply of distilled water, pipette filler bulb, graph paper. Chemicals required (general purpose reagent grade) Potassium dichromate K ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 20: Calculation Of Λ Max, Extinction Coefficient And Measurement Of Natural Bandwidth
Note that the absorbance plot is linear to about 1.5A and that the transmission plot is exponential. The flattening of the absorbance plot at higher values is ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 21: Measurement Of Stray Light
Therefore any transmission recorded at 340 nm will be a direct measurement of the stray light of the instrument. The value should be in accordance with the manufacturer’s specification. ___________________________________________________________________ Issue 02 - 03/2005 Libra S4, English... -
Page 22: Specification And Warranty
This product has been designed and manufactured by Biochrom Ltd, 22 Cambridge Science Park, Milton Road, Cambridge CB4 0FJ, UK.
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