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Libra S21 and S22
User Manual
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Summary of Contents for biochrom Libra S21

  • Page 1 Libra S21 and S22 User Manual English Deutsch Français Español Italiano...
  • Page 2 Libra S21 Visible and Libra 22 UV/Visible Spectrophotometers Part number 80-2115-25 and 80-2115-20 Serial number 81000 onwards manufactured by Biochrom Ltd. conforms to the requirements of the following Directives-: 73/23/EEC & 89/336/EEC Standards to which conformity is declared EN 61 010-1: 2001 Safety requirements for electrical equipment for measurement, control and laboratory use.
  • Page 5: Table Of Contents

    Other Accessories, consumables etc Acquire Applications Software MAINTENANCE After Sales Support Fuse Replacement Cleaning and General Care APPENDIX Text entry Good Laboratory Practice Least squares regression analysis and linearity SPECIFICATION AND WARRANTY ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 6: Unpacking, Positioning And Installation

    If this equipment is used in a manner not specified or in environmental conditions not appropriate for safe operation, the protection provided by the equipment may be impaired and instrument warranty withdrawn. ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 7: Essential Safety Notes

    Ensure that the cell compartment lid is closed when operating cell changers and the sipper. • It is essential that the baseplate plug supplied with single cell accessories is fitted to optimise air flow and to prevent light ingress. ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 8: Operation

    Self test diagnostics for GLP purposes A range of accessories further enhances the capability of the instrument. The home page provides access to user modes, system utilities and accessory identification and set-up. ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 9: Keypad And Display

    “escape mechanism” (red stop key) Press the corresponding number on the keypad to enter the user mode choices; for example 1 followed by 1 is Absorbance mode, whereas 2 followed by 4 is Standard Curve Mode. ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 10: Basic Modes

    This reference value is used for subsequent samples until changed • Insert samples as required and press (repeat as necessary) • To go back and change the wavelength or factor press Method (F1) ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 11: Ratio (1.4)

    This reference value is used for subsequent samples until changed • Insert samples as required and press (repeat as necessary) • To go back and change the wavelength or factor press Method (F1) ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 12: Applications

    For rapid movement, press 4 / 6 to go to left / right side of the graph, or 5 to go the centre • Press 2 to zoom in (8 to zoom out) • To go back and change the parameters press Method (F1) ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 13: Simple Kinetics (2.2)

    To go back and change the parameters press Method (F1) NOTE This mode should be used to check sample stabilisation prior to kinetics studies, for example, since the xenon lamp is not a continuous output source (unlike deuterium and tungsten lamps). ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 14: Reaction Rate (2.3)

    To see the assay on the whole display, press Graph (F3); to return press OK (F3) • Data points can be viewed by pressing Data (F1) moving the cursor (F2 and F1) • To go back and change the parameters press Method (F1) ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 15: Standard Curve (2.4)

    • This reference value is used for subsequent samples until changed • Insert samples as required and press (repeat as necessary) • To go back and change the parameters press Method (F1) ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 16: Multiwave And Equation Entry (2.5)

    • This reference value is used for subsequent samples until changed • Insert samples as required and press (repeat as necessary) • To go back and change the parameters press Method (F1) ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 17: Methods A (4), B (5) And C (6

    (eg 00), press > (F2) and then the appropriate key again. ← • Delete incorrect characters using • Complete entry by pressing OK (F3) • An example of name entry is given in the Appendix. ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 18: System Utilities

    • Default graph scale (0 – 3, 0 – 2, 0 – 1, 0 – 0.5 and Autoscale) • Confirm exit from application no / yes • Key click on / off ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 19 220 nm (<0.05%) 0.021 PASS Language (5) Select language for the display and print out. Service (6) This is for accredited service engineers only and requires the entry of a pass code. ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 20: Output To Printer

    For use with Epson 24 pin dot matrix printers and older inkjet printers such as the Stylus 400. Output is automatic when the key is pressed and auto-print (in Preferences) is on. If auto-print is off, results can be printed on demand using the key. ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 21: Download To Spreadsheet

    Failed to calibrate properly; contact local service engineer Failed to align filters Failed to calibrate properly; contact local service engineer Failed to align grating Failed to calibrate properly; contact local service engineer ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 22: Accessories

    6 position Peltier heated 80-2106-04 Requires Temperature Control Unit cell changer (80-2112-49). Insert into socket 1. 8 position cell changer 80-2108-01 Spare, if required ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 23: Single Cell Holder Accessories

    Peltier cell holder 80-2106-13 Set required temp in range 20-49 °C. Insert into socket 2. Set required temperature: off, 25, 30, Electrical cell holder 80-2106-12 37 °C. Insert into socket 2. ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 24: Other Accessories, Consumables Etc

    Centronics parallel printer interface cable 80-2071-87 Separate information giving details on serial and parallel interface connections, if required, is available from a Service Engineer with your local supplier, whom you should contact for further details. ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 25: Acquire Applications Software

    8MB RAM, 200Mb hard disk, a 1.44 MB 3.5 inch floppy disk drive, a serial mouse installed, and free COMMS serial port and VGA graphics. Any printer supported by Microsoft Windows 95 can be used. Contact your supplier for further information. ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 26: Maintenance

    Place fuses (1.0A, 5mm x 20mm, FST) into the fuse holder and slide back into position. Reconnect the power supply cord and switch on the instrument. Fuses are not normally consumed in an instrument's lifetime. If they blow repeatedly contact your supplier. ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 27: Cleaning And General Care

    Remove the cell holder and clean separately. • Use a soft dry cloth to mop out the sample compartment. Replace the cell holder. • Reconnect the power cord and switch on the instrument ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 28: Appendix

    Press F3 to confirm the equation is correct • The two wavelengths for A1 and A2 now have to be defined, enter 511 and 720 when prompted The dilution factor (*100) now has to be entered; enter 100 ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 29: Good Laboratory Practice

    If you are working in a regulated environment such as a drug discovery laboratory that generates data for GLP/GMP activities or reports, you should not use the instrument and contact your local service engineer. ___________________________________________________________________ Issue 02 - 04/2004 Libra S21/S22, English...
  • Page 30: Least Squares Regression Analysis And Linearity

    100%. It is used in both the Reaction Rate and Standard Curve modes, and is expressed by a coefficient of determination (r ), calculated using the following equation: ∑ ∑ ∑ − Quality ∑ ∑ ∑ ∑ − − ___________________________________________________________________ Libra S21/S22, English Issue 02 - 04/2004...
  • Page 31: Specification And Warranty

    They can accept no liability for loss or damage, however caused, arising from the faulty or incorrect use of this product. This product has been designed and manufactured by Biochrom Ltd, 22 Cambridge Science Park, Milton Road, Cambridge CB4 0FJ, UK.

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