Multi Laser Measurements - Partec CyFlow space Instrument Operating Manual

Operating Basics
DANGER
Warning: Biohazards!
The waste may contain biohazardeous and cancerogenic material from the samples (infectious cells, dyes).
To minimize biohazards, fill about 25 ml of Partec Hypochlorite Solution into the waste container. When
emptying the container, be sure not to come in contact with the fluid. In case of accidental contact, wash your
skin throughoutly with soap and disinfectant.

Multi laser Measurements

Notes
Alignment of the lasers
should only be done by
Partec representatives
or by your local
distributor.
Rev. 007 Date 2010-01-13
®
The CyFlow space Flow Cytometer can be equipped
with up to 3 laser light sources according to individual
customer demands. Actual setup of your instrument may
therefore vary. For questions concerning the laser setup
of this instrument please contact Partec or your local
distributor.
In order to minimize crosstalk between multiple light
sources, laser beams from different lasers are focussed
on different positions (spots) of the flow cuvette. The
®
CyFlow space Flow Cytometer supports either 2 laser
spots (when employing FloMax version 2.6 or 2.7) or 3
laser spots (when employing FloMax version 3.0). The
blue 488 nm laser which is the leading laser of the
®
CyFlow space is always located at spot 1. Additional
lasers if present are located on spot 2 and spot 3.
While the blue 488 nm laser is switched on with the
button on the left side of the instrument all other lasers
are controlled by software:
Select "Acquisition" from the upper command line and
click on Laser No. 2 and / or Laser No. 3 as required. The
tick indicates that a laser is switched on. Laser no. 2
would be a red laser (if present), laser no. 3 would be a
UV or violet laser (if present).
Laser status will automatically be saved upon creation of
Instrument settings files (please refer to the manual
®
– Acquisition and Instrument Control).
FloMax
In the standard configuration all particles pass through
laser spot no. 1 of the 488nm laser – the leading laser –
first and then through the laser(s) at spot no. 2 and no. 3
(if present).
Therefore, all parameters derived from a laser located at
a spot other than no. 1 require a time delay. Please refer
to Parameter Setup Dialog Box for details (Fig. 5, page
13).
®
CyFlow space Instrument Operating Manual
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