Optical Standard Setup - The Parameters - Partec CyFlow space Instrument Operating Manual

Operating Basics
Optical Standard Setup – The Parameters
Note
If required by a specific application, the optical
standard setup can be optimized by exchanging
preassembled removable mirror/filter blocks. This
is a matter of seconds and does not require
readjustments.
Laser 638 nm
SSC
D
FL1
D
FL4
D
D
FL2
D
FL3
Laser 638 nm
FL7
SSC
D
D
FL1
D
FL4
D
D
FL2
D
FL3
Fig. 2: Examplary demonstration of the optical
®
bench of a CyFlow
parameters (top) and 3 lasers and 9 parameters
(bottom). Other laser light sources and parameter
allocations possible. D detector (photo multiplier)
Rev. 007 Date 2010-01-13
Laser
488 nm
M
Laser
CCD
optic
Flow cell
D M
FL5
D
FSC
D
FL6
Laser
488 nm
Laser
405
nm
M
M
Laser
CCD
optic
Flow cell
D M
FL5
D
FSC
D
FL6
space with 2 lasers and 8
®
The CyFlow space flow cytometer can be
equipped with various laser light sources (1, 2 or 3
lasers), different laser power intensities and up to
nine optical parameters.
Due to its modular
configuration can be adopted to many different
clinical and scientific
standard configurations include:
2 Laser instrument: This instrument is equipped
with a blue diode pumped solid-state Laser (20
mW) at 488 nm and a red laser diode at 638 nm
(25 mW). Up to eight optical parameters can be
included (colours reflect laser origin of the signal).
FSC: forward scatter
SSC: side scatter
FL1: green fluorescence (FITC)
FL2: orange fluorescence (PE)
FL3: red fluorescence I (PE-Cy5)
FL4: far red fluorescence I (PE-Cy7)
FL5: red fluorescence II (APC)
FL6: far red fluorescence II (APC-Cy7)
Other parameter allocations possible
3 Laser instrument, high power laser 488 nm:
This instrument is equipped with a blue diode
pumped solid-state Laser (200 mW) at 488 nm, a
violet laser diode at 405 nm (100 mW) and a red
laser diode at 638 nm (25 mW). Up to nine optical
parameters can be included (colours in the table
below reflect laser origin of the signal).
FSC: forward scatter
SSC: side scatter
FL1: green fluorescence (FITC)
FL2: orange fluorescence (PE)
FL3: red fluorescence I (PE-Cy5)
FL4: far red fluorescence I (PE-Cy7)
FL5: red fluorescence II (APC)
FL6: far red fluorescence II (APC-Cy7)
FL7: blue fluorescence (CFP, Alexa 405)
Other parameter allocations possible
Other lasers available:
532 nm green solid state laser (50 or 100 mW)
375 nm UV laser diode (16 mW)
More lasers on request
The instrument is equipped with a CCD camera in
order to monitor the particle flow and to check the
focus of the objective.
®
CyFlow space Instrument Operating Manual
concept the optical
purposes. The main
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