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Important! Do not fill the base
with commercial antifreeze,
organic solvents, or pure
water.
Note: It is not necessary to
replace the coolant.
Operating instructions
Agarose gels are fi rst prepared using the gel cast-
ing kit. Samples are then loaded into wells and
electrophoretically separated. The fl uorescent
dye ethidium bromide can be added to the gel or
electrophoresis buffer or both to track separa-
tion progress. After electrophoresis, the gel may
be stained and photographed, blotted, or dried
for autoradiography.
Fill the base with coolant
Even if no cooling is required, it is important to fill
the base with the proper coolant solution before the
first use because the solution provides a necessary
heat sink.
Prepare 600 ml of 50/50 ethylene glycol/water.
Optional: To help see wells more clearly while loading
the sample, add a drop or two of soluble dye or food
color to the coolant solution.
Locate the two inlet holes in the top edge of the base.
Fill the base cavity as full as possible with coolant
using a 50-ml syringe or a pump.
Push a gray rubber plug into each hole, taking care
that the plug is securely seated.
Place the prepared base in an ice bucket or into a
refrigerator or freezer set no lower than -20 °C for
about an hour before use. (The base will always be
ready if you store it in the refrigerator or freezer.)
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