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Cast the gel
Install the running tray.
Firmly grasp the casting tray with one hand. With
the other hand, place one end of the running tray
against the foam pad at the bottom edge, press the
tray against the pad, and then lower it to rest on the
bottom of the casting tray, seating the other end of
the tray against the opposite foam pad.
Prepare the comb(s).
Fit the two slots in the comb between the
(loosened) thumb screw heads and the comb back.
Tighten the screws until the comb is just supported.
Seat the comb assembly on the rim of the casting
tray and adjust the bottom of the comb so that it
is about 1.0 mm from the running tray. Tighten the
screws to secure the comb. To run twice as many
samples, prepare two combs.
Remove the comb assembly. Place the casting
assembly on a leveling surface and level, using the
spirit level on the running tray as a guide.
Pour the agarose solution (cooled to 50 °C) into
the casting tray. Orient the comb assembly so that
the comb faces the nearest foam pad and seat it
on the tray rim. Check that the comb is vertical to
prevent well shape distortions. To run twice as many
samples, place the second comb assembly in the
center of the tray. Allow a minimum of 30 minutes
for the gel to set.
Once the gel is set, remove the comb carefully.
Partially lift and slightly tilt the comb at one end
and then slowly withdraw it from the gel. (Pulling
the comb straight up creates a vacuum in the wells
that may lift the gel out of the tray.)
p7
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