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Operating instructions
Gel casting and electrophoresis procedures
follow. Included are instructions for polyacryl-
amide gels (used with continuous or discon-
tinuous buffer systems) and gradient gels. See
page 31 for bibliography.
Prepare the gel sandwich
Glass plates, spacers, and clamp sets are sized
so that the assembled sandwich can be easily
aligned to create the seal required first to cast the
gel and then to run it. For best results take extra
care to align all components when assembling
sandwiches. One to four gels (18 × 16 cm) can
be assembled and run in the SE 600 Chroma.
Both precast gels and self-cast gels can be used.
To self-cast multiple gels, kits can be ordered
separately: The SE 615 Multiple Gel Caster Kit
holds up to 10 single gel sandwiches, and the SE
675 Gel Caster Kit holds up to four sandwiches.
(See the accompanying gel caster User Manual
for complete instructions.) To run four gels con-
currently, two accessory notched divider plates
and two additional pairs of spacers are required.
Construct the gel sandwich and insert into
caster
Prepare the caster and clamps
Place the spirit level into the caster center and adjust the leveling
feet. Loosen all clamp screws and make space for the sandwich by
sliding the pressure plates toward the screws.
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