Table of Contents
Advertisement
Caution: Ethidium bromide is a
known mutagen. Always wear
gloves when handling.
Fig 2. Gel casting kit.
Approach the foam pad with one
end of the running tray and then
gently press the tray edge against
the pad, compressing it enough
to allow the opposite end of the
running tray to drop fully into the
casting tray before sealing against
the foam pad.
foam pads (2)
p6
•
Prepare solutions
Prepare 250 ml of running buffer. (Refer to p. 12 for
recipes of commonly used electrophoretic running
buffers.)
Prepare the sample loading buffer. (Refer to p. 13 for
a recipe and a table of volume requirements for each
comb size.)
Prepare approximately 7 ml agarose solution per mm
of gel thickness. (For example, a 3-mm gel requires
0.3 cm × 7 cm × 10 cm = 21 ml)
Dissolve agarose in running buffer, heat according
to instructions accompanying the agarose, and allow
the solution to cool to 50 °C before pouring into the
casting tray.
Optional: Add 0.5 µg/ml ethidium bromide to the gel
solution to observe separation during electrophoresis.
UV-transparent running tray
(cast the gel on this tray, then
transfer gel to the horizontal unit
base for electrophoresis.)
gel casting tray
Advertisement
Table of Contents
