Bibliography - Hoefer HE33 User Manual

H2O) for 15 to 60 minutes and then view or
photograph the sample on a UV transilluminator.
To photograph the gel, either place the running
tray on the transilluminator surface or slide
the gel onto the surface for maximum expo-
sure. (The running tray is 95% transparent to
302-nm light and 40% transparent to 254 nm
light.) View the sample under 366-nm UV light
or reduced intensity 302-nm UV light to reduce
photonicking.
To reduce the background fl uorescence of
unbound ethidium bromide, the gel can be
destained by soaking it for 5 minutes in 0.01
m MgCl , or for 1 hour in 0.001 M MgSO .
2 4
Destaining makes it easier to detect small quan-
tities (less than 10 ng) of DNA. (Sambrook,
section 6.15)

Bibliography

Ausubel, et al., (eds). Current Protocols in
Molecular Biology. Greene Publishing and
Wiley-Interscience. New York (1993).
Sambrook, J., Fritsch, E.F., and Maniatis, T.,
Molecular Cloning:
A Laboratory Manual. Cold Spring Harbor
Laboratory Press (1989).
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